CA2493849A1 - Procedes et systemes de culture in vitro etendue de cellules neuronales - Google Patents
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- CA2493849A1 CA2493849A1 CA002493849A CA2493849A CA2493849A1 CA 2493849 A1 CA2493849 A1 CA 2493849A1 CA 002493849 A CA002493849 A CA 002493849A CA 2493849 A CA2493849 A CA 2493849A CA 2493849 A1 CA2493849 A1 CA 2493849A1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
- A61P27/06—Antiglaucoma agents or miotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/062—Sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/02—Drug screening
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Neurology (AREA)
- Ophthalmology & Optometry (AREA)
- Biochemistry (AREA)
- Neurosurgery (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Acoustics & Sound (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cardiology (AREA)
- Vascular Medicine (AREA)
- Urology & Nephrology (AREA)
- Heart & Thoracic Surgery (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un système de culture cellulaire se rapportant à la culture in vitro étendue de cellules neuronales matures, ainsi que des procédés de préparation de ce système. Dans un mode de réalisation préféré, l'invention concerne un système de culture cellulaire comprenant un mélange de cellules neuronales matures de la rétine et de cellules isolées d'un corps ciliaire. L'invention concerne également des procédés permettant d'identifier des agents bioactifs qui modifient la neurodégénération des cellules neuronales de la rétine.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39597302P | 2002-07-12 | 2002-07-12 | |
| US60/395,973 | 2002-07-12 | ||
| PCT/US2003/021968 WO2004007749A2 (fr) | 2002-07-12 | 2003-07-11 | Procedes et systemes de culture in vitro etendue de cellules neuronales |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2493849A1 true CA2493849A1 (fr) | 2004-01-22 |
Family
ID=30115948
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002493849A Abandoned CA2493849A1 (fr) | 2002-07-12 | 2003-07-11 | Procedes et systemes de culture in vitro etendue de cellules neuronales |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US7312025B2 (fr) |
| EP (1) | EP1551956A4 (fr) |
| JP (1) | JP2006506970A (fr) |
| AU (1) | AU2003249227A1 (fr) |
| CA (1) | CA2493849A1 (fr) |
| WO (1) | WO2004007749A2 (fr) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2006506970A (ja) * | 2002-07-12 | 2006-03-02 | ユニバーシティ オブ ワシントン | 神経細胞の延長されたインビトロ培養のための方法および系 |
| EP1649009A2 (fr) * | 2003-07-30 | 2006-04-26 | Acucela, Inc. | Culture etendue de cellules retiennes primaires et modeles de stress, ainsi que procedes d'utilisation associes |
| WO2005056174A2 (fr) * | 2003-12-02 | 2005-06-23 | Ppg Industries Ohio, Inc. | Sols de particules colloidales et leurs procedes de preparation |
| US7541186B2 (en) * | 2006-02-22 | 2009-06-02 | University Of Washington | Method of generating human retinal progenitors from embryonic stem cells |
| US9061017B2 (en) | 2006-05-03 | 2015-06-23 | The Schepens Eye Research Institute, Inc. | Isolation and therapeutic application of adult retinal stem cells collected from extra-retinal tissues |
| WO2007130060A2 (fr) | 2006-05-03 | 2007-11-15 | Schepens Eye Research | Isolation et application thérapeutique de cellules souches rétiniennes adultes prélevées sur des tissus extra-rétiniens |
| JP5371953B2 (ja) | 2007-04-20 | 2013-12-18 | アキュセラ インコーポレイテッド | 眼の疾患及び障害を治療するスチレニル誘導体化合物 |
| WO2009005794A2 (fr) | 2007-06-29 | 2009-01-08 | Acucela, Inc. | Dérivés d'alcynylphényle pour traiter les maladies et les affections ophtalmiques |
| RU2014151622A (ru) | 2007-10-05 | 2015-08-20 | Акусела Инк. | Композиции и способы лечения нейродегенеративных заболеваний |
| US20120058099A1 (en) * | 2009-03-04 | 2012-03-08 | Children's Medical Center Corporation | Use of hypoxanthine for promotion of neuronal outgrowth |
| ES2712060T3 (es) * | 2010-04-13 | 2019-05-09 | M Lab Gmbh | Métodos de diagnóstico para el glaucoma |
| WO2013086199A1 (fr) * | 2011-12-07 | 2013-06-13 | Huang Lotien R | Procédé et dispositif pour l'isolement des cellules non graisseuses d'un tissu adipeux |
| CN104703598A (zh) | 2012-01-20 | 2015-06-10 | 奥克塞拉有限公司 | 用于疾病治疗的取代的杂环化合物 |
| EP2970099A4 (fr) | 2013-03-12 | 2016-12-21 | Acucela Inc | Dérivés de 3-phénylpropylamine substitués pour le traitement des maladies et des troubles ophtalmiques |
| US11241460B2 (en) | 2013-03-15 | 2022-02-08 | Astellas Institute For Regenerative Medicine | Photoreceptors and photoreceptor progenitors produced from pluripotent stem cells |
| CA2937129A1 (fr) | 2014-01-17 | 2015-07-23 | Sumitomo Chemical Company, Limited | Procede d'obtention de cellules souches de bord ciliaire |
Family Cites Families (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2461002A1 (fr) | 1979-07-13 | 1981-01-30 | Inst Nat Sante Rech Med | Procede pour stimuler la croissance des cellules d'epiderme humain et produits faisant application dudit procede |
| US6048728A (en) | 1988-09-23 | 2000-04-11 | Chiron Corporation | Cell culture medium for enhanced cell growth, culture longevity, and product expression |
| US5851832A (en) | 1991-07-08 | 1998-12-22 | Neurospheres, Ltd. | In vitro growth and proliferation of multipotent neural stem cells and their progeny |
| JPH05123168A (ja) | 1991-10-30 | 1993-05-21 | Green Cross Corp:The | 細胞長期培養方法 |
| US6319687B1 (en) | 1992-09-24 | 2001-11-20 | The United States Of America As Represented By The Department Of Health And Human Services | Pigment epithelium-derived factor: characterization, genomic organization and sequence of PEDF gene |
| US5840686A (en) | 1992-09-24 | 1998-11-24 | The United States Of America As Represented By The Department Of Health And Human Services | Pigment epithelium-derived factor: characterization of its novel biological activity and sequences encoding and expressing the protein and methods of use |
| JP2755880B2 (ja) | 1992-09-11 | 1998-05-25 | 住友ベークライト株式会社 | 培養用器具及びその製造方法 |
| JPH07289249A (ja) | 1994-04-26 | 1995-11-07 | Bio Material Kenkyusho:Kk | 神経細胞用培地及びその培養方法 |
| WO2001009327A2 (fr) | 1999-07-28 | 2001-02-08 | Genentech, Inc. | Procede de prevention de la deterioration ou de la mort des cellules de la retine et de traitement des troubles oculaires |
| JP3093974B2 (ja) | 1995-06-27 | 2000-10-03 | 住友ベークライト株式会社 | 神経細胞用培養液、その製造方法及びこれを用いる神経細胞の培養方法 |
| WO1997004733A1 (fr) | 1995-07-27 | 1997-02-13 | Taisei Kako Co., Ltd. | Doseur buccal |
| US5641750A (en) | 1995-11-29 | 1997-06-24 | Amgen Inc. | Methods for treating photoreceptors using glial cell line-derived neurotrophic factor (GDNF) protein product |
| FR2747690B1 (fr) | 1996-04-19 | 1998-06-12 | Neurotech Sa | Lignees immortalisees de cellules retiniennes et leurs applications au traitement de differents troubles primaires ou secondaires ophtalmologiques ou neurologiques |
| US6878544B2 (en) | 1996-04-19 | 2005-04-12 | Neurotech Sa | Retinal cell lines with extended life-span and their applications |
| US6090624A (en) | 1996-04-19 | 2000-07-18 | Brown University Research Foundation | Immortalized retinal cell lines and their applications |
| JPH09295976A (ja) | 1996-05-01 | 1997-11-18 | Kirin Brewery Co Ltd | 神経保護作用物質ｃｕ３９、その製造法および使用 |
| US5753506A (en) | 1996-05-23 | 1998-05-19 | Cns Stem Cell Technology, Inc. | Isolation propagation and directed differentiation of stem cells from embryonic and adult central nervous system of mammals |
| AUPO235996A0 (en) | 1996-09-16 | 1996-10-10 | Garvan Institute Of Medical Research | Method of cell culture |
| CA2216439A1 (fr) | 1996-09-25 | 1998-03-25 | Derek Van Der Kooy | Produits pharmaceutiques contenant des cellules souches retiniennes |
| US6121231A (en) | 1996-12-06 | 2000-09-19 | Institut Pasteur | Use of the KAL protein and treatment with the KAL protein in treatment of retinal, renal, neuromal and neural injury |
| JPH10257891A (ja) | 1997-03-19 | 1998-09-29 | Taisho Pharmaceut Co Ltd | 神経細胞機能賦活化活性を有する新規蛋白質ｈｕｃｅｐ−１ |
| JPH1132769A (ja) | 1997-07-22 | 1999-02-09 | Taisho Pharmaceut Co Ltd | 新規遺伝子とそれにコードされる蛋白質 |
| US5968829A (en) | 1997-09-05 | 1999-10-19 | Cytotherapeutics, Inc. | Human CNS neural stem cells |
| WO1999013074A1 (fr) | 1997-09-08 | 1999-03-18 | Taisho Pharmaceutical Co., Ltd. | Proteine activant des fonctions de cellules nerveuses |
| AU1996599A (en) | 1997-12-05 | 1999-06-28 | Board Of Trustees Of The Leland Stanford Junior University | Long-term survival and regeneration of central nervous system neurons |
| AU759273B2 (en) | 1998-01-02 | 2003-04-10 | Titan Pharmaceuticals, Inc. | Use of retinal pigment epithelial cells for creation of an immune privilege site |
| JPH11292774A (ja) | 1998-04-08 | 1999-10-26 | Agriculture Forestry And Fisheries Technical Information Society | 神経細胞生存維持剤 |
| US6284245B1 (en) | 1998-08-25 | 2001-09-04 | Diacrin, Inc. | Neural retinal cells and retinal pigment epithelium cells and their use in treatment of retinal disorders |
| US6379882B1 (en) | 1998-09-14 | 2002-04-30 | Elan Pharmaceuticals, Inc. | Method for selecting compounds for treating ischemia-related cellular damage |
| JP3141107B2 (ja) | 1998-11-16 | 2001-03-05 | 工業技術院長 | ヒト由来ブラディオン蛋白質、それをコードするｄｎａ及びそれらの使用 |
| US6610540B1 (en) | 1998-11-18 | 2003-08-26 | California Institute Of Technology | Low oxygen culturing of central nervous system progenitor cells |
| ATE402253T1 (de) | 1999-01-05 | 2008-08-15 | Aventis Pharma Sa | Humane adulte astrozyten, sowie deren herstellung und verwendung |
| JP2002536423A (ja) | 1999-02-11 | 2002-10-29 | ザ スキーペンズ アイ リサーチ インスティテュート インコーポレイテッド | 未成熟及び成熟ジストロフィーレシピエントの神経組織内に移植された神経前駆細胞の統合 |
| US20030003572A1 (en) | 1999-03-05 | 2003-01-02 | David J. Anderson | Isolation and enrichment of neural stem cells from uncultured tissue based on cell-surface marker expression |
| EP1107003A1 (fr) | 1999-12-09 | 2001-06-13 | Trophos | Méthodes pour criblage des composés avec activité sur les neurones |
| US6406840B1 (en) | 1999-12-17 | 2002-06-18 | Biomosaic Systems, Inc. | Cell arrays and the uses thereof |
| JP2003521910A (ja) * | 2000-02-11 | 2003-07-22 | ザ スキーペンズ アイ リサーチ インスティテュート インコーポレイテッド | 網膜幹細胞の分離及び移植 |
| EP1130096A1 (fr) | 2000-03-03 | 2001-09-05 | Institut de la Santé et de la Recherche Médicale | Facteur de transcription (PATF), qui induit l'arret de la proliferation |
| JP4317310B2 (ja) | 2000-03-09 | 2009-08-19 | 三菱化学株式会社 | 神経細胞死抑制作用を有する薬剤及びそのスクリーニング方法 |
| JP2001299334A (ja) | 2000-04-20 | 2001-10-30 | Sumitomo Bakelite Co Ltd | 細胞培養液、細胞培養方法及びバイオアッセイ方法 |
| WO2001083714A2 (fr) | 2000-05-02 | 2001-11-08 | Central Institute For The Deaf | Composition et procedes servant a traiter la degenerescence de photorecepteurs |
| US20020009713A1 (en) | 2000-05-11 | 2002-01-24 | Miller Freda D. | Methods for identifying modulators of neuronal growth |
| FR2815042B1 (fr) | 2000-10-06 | 2005-01-07 | Haguenauer Odile Cohen | Utilisation d'une composition a activite antioxydante pour le conditionnement de cellules souches |
| US6498108B2 (en) | 2001-02-12 | 2002-12-24 | Fairchild Semiconductor Corporation | Method for removing surface contamination on semiconductor substrates |
| WO2002076386A2 (fr) | 2001-03-26 | 2002-10-03 | Zymogenetics, Inc. | Procede permettant d'induire la proliferation de cellules souches retiniennes |
| JP2006506970A (ja) * | 2002-07-12 | 2006-03-02 | ユニバーシティ オブ ワシントン | 神経細胞の延長されたインビトロ培養のための方法および系 |
-
2003
- 2003-07-11 JP JP2004521788A patent/JP2006506970A/ja not_active Ceased
- 2003-07-11 AU AU2003249227A patent/AU2003249227A1/en not_active Abandoned
- 2003-07-11 US US10/618,076 patent/US7312025B2/en not_active Expired - Fee Related
- 2003-07-11 CA CA002493849A patent/CA2493849A1/fr not_active Abandoned
- 2003-07-11 EP EP03764619A patent/EP1551956A4/fr not_active Withdrawn
- 2003-07-11 WO PCT/US2003/021968 patent/WO2004007749A2/fr active Search and Examination
-
2007
- 2007-11-16 US US11/985,856 patent/US20080175826A1/en not_active Abandoned
-
2010
- 2010-08-23 US US12/861,754 patent/US20110044957A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20080175826A1 (en) | 2008-07-24 |
| US20040147019A1 (en) | 2004-07-29 |
| WO2004007749A2 (fr) | 2004-01-22 |
| US20110044957A1 (en) | 2011-02-24 |
| JP2006506970A (ja) | 2006-03-02 |
| AU2003249227A8 (en) | 2004-02-02 |
| AU2003249227A1 (en) | 2004-02-02 |
| US7312025B2 (en) | 2007-12-25 |
| EP1551956A2 (fr) | 2005-07-13 |
| EP1551956A4 (fr) | 2006-12-27 |
| WO2004007749A3 (fr) | 2005-05-06 |
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| EEER | Examination request | ||
| FZDE | Discontinued |