CA2482173C - Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells - Google Patents

Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells Download PDF

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Publication number
CA2482173C
CA2482173C CA2482173A CA2482173A CA2482173C CA 2482173 C CA2482173 C CA 2482173C CA 2482173 A CA2482173 A CA 2482173A CA 2482173 A CA2482173 A CA 2482173A CA 2482173 C CA2482173 C CA 2482173C
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Canada
Prior art keywords
bacteriophage
target bacterium
target
progeny
exposed sample
Prior art date
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Expired - Fee Related
Application number
CA2482173A
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English (en)
French (fr)
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CA2482173A1 (en
Inventor
Angelo J. Madonna
Kent J. Voorhees
Jon C. Rees
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Colorado School of Mines
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Colorado School of Mines
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Publication of CA2482173A1 publication Critical patent/CA2482173A1/en
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Publication of CA2482173C publication Critical patent/CA2482173C/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326Magnetic particles
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911Bacteria

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2482173A 2002-04-12 2003-04-10 Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells Expired - Fee Related CA2482173C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31918402P 2002-04-12 2002-04-12
US60/319,184 2002-04-12
PCT/US2003/011253 WO2003087772A2 (en) 2002-04-12 2003-04-10 Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells

Publications (2)

Publication Number Publication Date
CA2482173A1 CA2482173A1 (en) 2003-10-23
CA2482173C true CA2482173C (en) 2012-10-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2482173A Expired - Fee Related CA2482173C (en) 2002-04-12 2003-04-10 Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells

Country Status (9)

Country Link
US (3) US7166425B2 (enExample)
EP (1) EP1540018B1 (enExample)
JP (2) JP4490112B2 (enExample)
AT (1) ATE469240T1 (enExample)
AU (1) AU2003228505B2 (enExample)
CA (1) CA2482173C (enExample)
DE (1) DE60332740D1 (enExample)
ES (1) ES2346424T3 (enExample)
WO (1) WO2003087772A2 (enExample)

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US20070275370A1 (en) 2007-11-29
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US7972773B2 (en) 2011-07-05
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US20040224359A1 (en) 2004-11-11
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