CA2417986C - Single-labeled oligonucleotide probes - Google Patents
Single-labeled oligonucleotide probes Download PDFInfo
- Publication number
- CA2417986C CA2417986C CA2417986A CA2417986A CA2417986C CA 2417986 C CA2417986 C CA 2417986C CA 2417986 A CA2417986 A CA 2417986A CA 2417986 A CA2417986 A CA 2417986A CA 2417986 C CA2417986 C CA 2417986C
- Authority
- CA
- Canada
- Prior art keywords
- probe
- nucleic acid
- target nucleic
- hybridization
- oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108020005187 Oligonucleotide Probes Proteins 0.000 title claims abstract description 32
- 239000002751 oligonucleotide probe Substances 0.000 title claims abstract description 32
- 239000000523 sample Substances 0.000 claims abstract description 293
- 150000007523 nucleic acids Chemical group 0.000 claims abstract description 113
- 238000009396 hybridization Methods 0.000 claims abstract description 96
- 238000000034 method Methods 0.000 claims abstract description 59
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 50
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 50
- 230000003321 amplification Effects 0.000 claims abstract description 41
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 41
- 238000010494 dissociation reaction Methods 0.000 claims abstract description 26
- 230000005593 dissociations Effects 0.000 claims abstract description 26
- 238000012544 monitoring process Methods 0.000 claims abstract description 26
- 239000007850 fluorescent dye Substances 0.000 claims description 78
- 108091034117 Oligonucleotide Proteins 0.000 claims description 70
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 56
- 239000013615 primer Substances 0.000 claims description 52
- 230000000295 complement effect Effects 0.000 claims description 40
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 40
- 239000002773 nucleotide Substances 0.000 claims description 35
- 125000003729 nucleotide group Chemical group 0.000 claims description 35
- 238000000137 annealing Methods 0.000 claims description 27
- 238000010791 quenching Methods 0.000 claims description 26
- 230000000171 quenching effect Effects 0.000 claims description 26
- 239000012472 biological sample Substances 0.000 claims description 24
- 230000001419 dependent effect Effects 0.000 claims description 22
- 239000000975 dye Substances 0.000 claims description 21
- OZFPSOBLQZPIAV-UHFFFAOYSA-N 5-nitro-1h-indole Chemical compound [O-][N+](=O)C1=CC=C2NC=CC2=C1 OZFPSOBLQZPIAV-UHFFFAOYSA-N 0.000 claims description 18
- 239000007983 Tris buffer Substances 0.000 claims description 17
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 17
- 239000000872 buffer Substances 0.000 claims description 16
- LAVZKLJDKGRZJG-UHFFFAOYSA-N 4-nitro-1h-indole Chemical compound [O-][N+](=O)C1=CC=CC2=C1C=CN2 LAVZKLJDKGRZJG-UHFFFAOYSA-N 0.000 claims description 15
- PSWCIARYGITEOY-UHFFFAOYSA-N 6-nitro-1h-indole Chemical compound [O-][N+](=O)C1=CC=C2C=CNC2=C1 PSWCIARYGITEOY-UHFFFAOYSA-N 0.000 claims description 15
- LOJNBPNACKZWAI-UHFFFAOYSA-N 3-nitro-1h-pyrrole Chemical compound [O-][N+](=O)C=1C=CNC=1 LOJNBPNACKZWAI-UHFFFAOYSA-N 0.000 claims description 14
- 229930010555 Inosine Natural products 0.000 claims description 13
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims description 13
- MRWXACSTFXYYMV-UHFFFAOYSA-N Nebularine Natural products OC1C(O)C(CO)OC1N1C2=NC=NC=C2N=C1 MRWXACSTFXYYMV-UHFFFAOYSA-N 0.000 claims description 13
- 229960003786 inosine Drugs 0.000 claims description 13
- MRWXACSTFXYYMV-FDDDBJFASA-N nebularine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC=C2N=C1 MRWXACSTFXYYMV-FDDDBJFASA-N 0.000 claims description 13
- 150000001768 cations Chemical class 0.000 claims description 10
- -1 5-iodo-2'-cytidine Chemical compound 0.000 claims description 9
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine group Chemical group [C@@H]1([C@H](O)[C@H](O)[C@@H](CO)O1)N1C=NC=2C(N)=NC=NC12 OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims description 6
- 108091033319 polynucleotide Proteins 0.000 claims description 6
- 102000040430 polynucleotide Human genes 0.000 claims description 6
- 239000002157 polynucleotide Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical class [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims description 6
- 239000003155 DNA primer Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 claims description 4
- 238000007846 asymmetric PCR Methods 0.000 claims description 4
- 230000001747 exhibiting effect Effects 0.000 claims description 4
- CBTVGIZVANVGBH-UHFFFAOYSA-N aminomethyl propanol Chemical compound CC(C)(N)CO CBTVGIZVANVGBH-UHFFFAOYSA-N 0.000 claims description 3
- 230000001404 mediated effect Effects 0.000 claims description 3
- 229940058020 2-amino-2-methyl-1-propanol Drugs 0.000 claims description 2
- UXFQFBNBSPQBJW-UHFFFAOYSA-N 2-amino-2-methylpropane-1,3-diol Chemical compound OCC(N)(C)CO UXFQFBNBSPQBJW-UHFFFAOYSA-N 0.000 claims description 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 2
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 claims description 2
- 239000007997 Tricine buffer Substances 0.000 claims description 2
- 229960005305 adenosine Drugs 0.000 claims description 2
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 claims description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 claims 7
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 claims 3
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 claims 3
- 229940029575 guanosine Drugs 0.000 claims 3
- 108010066717 Q beta Replicase Proteins 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 23
- 238000004458 analytical method Methods 0.000 abstract description 21
- 238000011880 melting curve analysis Methods 0.000 abstract description 17
- 238000003205 genotyping method Methods 0.000 abstract description 16
- 238000011002 quantification Methods 0.000 abstract description 5
- 244000052769 pathogen Species 0.000 abstract description 4
- 230000001717 pathogenic effect Effects 0.000 abstract description 3
- 230000004044 response Effects 0.000 abstract description 2
- 108020004414 DNA Proteins 0.000 description 83
- 241000282414 Homo sapiens Species 0.000 description 58
- 239000002585 base Substances 0.000 description 51
- 238000003752 polymerase chain reaction Methods 0.000 description 46
- 238000002844 melting Methods 0.000 description 45
- 230000008018 melting Effects 0.000 description 45
- 230000008859 change Effects 0.000 description 36
- 230000035772 mutation Effects 0.000 description 34
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 17
- 238000003556 assay Methods 0.000 description 16
- 230000007704 transition Effects 0.000 description 16
- 241000607149 Salmonella sp. Species 0.000 description 14
- 230000000694 effects Effects 0.000 description 14
- 241000607142 Salmonella Species 0.000 description 13
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 11
- 238000004925 denaturation Methods 0.000 description 10
- 230000036425 denaturation Effects 0.000 description 10
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 10
- 108700028369 Alleles Proteins 0.000 description 9
- 239000012634 fragment Substances 0.000 description 9
- 238000010438 heat treatment Methods 0.000 description 9
- 108010014172 Factor V Proteins 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 8
- 108010091897 factor V Leiden Proteins 0.000 description 8
- 108010094028 Prothrombin Proteins 0.000 description 7
- 102200071038 rs1800562 Human genes 0.000 description 7
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 6
- 108010085686 Hemoglobin C Proteins 0.000 description 6
- 230000003416 augmentation Effects 0.000 description 6
- 229940098773 bovine serum albumin Drugs 0.000 description 6
- 238000001816 cooling Methods 0.000 description 5
- 125000005647 linker group Chemical group 0.000 description 5
- 201000003883 Cystic fibrosis Diseases 0.000 description 4
- 208000018565 Hemochromatosis Diseases 0.000 description 4
- 108010030837 Methylenetetrahydrofolate Reductase (NADPH2) Proteins 0.000 description 4
- 102000005954 Methylenetetrahydrofolate Reductase (NADPH2) Human genes 0.000 description 4
- 230000027455 binding Effects 0.000 description 4
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 4
- 230000001687 destabilization Effects 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 102200071191 rs1799945 Human genes 0.000 description 4
- 101100011886 Arabidopsis thaliana SQE4 gene Proteins 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 102100027378 Prothrombin Human genes 0.000 description 3
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 238000001506 fluorescence spectroscopy Methods 0.000 description 3
- 150000008300 phosphoramidites Chemical class 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 229940039716 prothrombin Drugs 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 102100023340 3-ketodihydrosphingosine reductase Human genes 0.000 description 2
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 2
- 101100533757 Caenorhabditis elegans snf-3 gene Proteins 0.000 description 2
- 102100024533 Carcinoembryonic antigen-related cell adhesion molecule 1 Human genes 0.000 description 2
- 102100024106 Cyclin-Y Human genes 0.000 description 2
- 230000006820 DNA synthesis Effects 0.000 description 2
- 108060002716 Exonuclease Proteins 0.000 description 2
- 206010058279 Factor V Leiden mutation Diseases 0.000 description 2
- 101001050680 Homo sapiens 3-ketodihydrosphingosine reductase Proteins 0.000 description 2
- 101000947157 Homo sapiens CXXC-type zinc finger protein 1 Proteins 0.000 description 2
- 101000981093 Homo sapiens Carcinoembryonic antigen-related cell adhesion molecule 1 Proteins 0.000 description 2
- 101000910602 Homo sapiens Cyclin-Y Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 101000896027 Rattus norvegicus 3-hydroxyacyl-CoA dehydrogenase Proteins 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- 239000010432 diamond Substances 0.000 description 2
- 102000013165 exonuclease Human genes 0.000 description 2
- 101150023545 gel1 gene Proteins 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000002777 nucleoside Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- VGIRNWJSIRVFRT-UHFFFAOYSA-N 2',7'-difluorofluorescein Chemical compound OC(=O)C1=CC=CC=C1C1=C2C=C(F)C(=O)C=C2OC2=CC(O)=C(F)C=C21 VGIRNWJSIRVFRT-UHFFFAOYSA-N 0.000 description 1
- YKBGVTZYEHREMT-KVQBGUIXSA-N 2'-deoxyguanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 YKBGVTZYEHREMT-KVQBGUIXSA-N 0.000 description 1
- CKTSBUTUHBMZGZ-ULQXZJNLSA-N 4-amino-1-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-tritiopyrimidin-2-one Chemical compound O=C1N=C(N)C([3H])=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-ULQXZJNLSA-N 0.000 description 1
- NJYVEMPWNAYQQN-UHFFFAOYSA-N 5-carboxyfluorescein Chemical group C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C21OC(=O)C1=CC(C(=O)O)=CC=C21 NJYVEMPWNAYQQN-UHFFFAOYSA-N 0.000 description 1
- 239000012103 Alexa Fluor 488 Substances 0.000 description 1
- 101100345318 Arabidopsis thaliana MFP2 gene Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 101150092286 CFT2 gene Proteins 0.000 description 1
- 101100328884 Caenorhabditis elegans sqt-3 gene Proteins 0.000 description 1
- 241000193155 Clostridium botulinum Species 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 1
- 241001646719 Escherichia coli O157:H7 Species 0.000 description 1
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 1
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 208000033981 Hereditary haemochromatosis Diseases 0.000 description 1
- 101000587058 Homo sapiens Methylenetetrahydrofolate reductase Proteins 0.000 description 1
- 238000009015 Human TaqMan MicroRNA Assay kit Methods 0.000 description 1
- 108010013563 Lipoprotein Lipase Proteins 0.000 description 1
- 241000186779 Listeria monocytogenes Species 0.000 description 1
- 102100029684 Methylenetetrahydrofolate reductase Human genes 0.000 description 1
- 101000586272 Mus musculus Osteocalcin-2 Proteins 0.000 description 1
- 101100273988 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) paa-3 gene Proteins 0.000 description 1
- 101100076847 Oryza sativa subsp. japonica MFT2 gene Proteins 0.000 description 1
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 1
- 101100345756 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) MMT1 gene Proteins 0.000 description 1
- 101100345757 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) MMT2 gene Proteins 0.000 description 1
- 101100043373 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SQT1 gene Proteins 0.000 description 1
- 241001138501 Salmonella enterica Species 0.000 description 1
- 241000239226 Scorpiones Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 108010016797 Sickle Hemoglobin Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- MZZINWWGSYUHGU-UHFFFAOYSA-J ToTo-1 Chemical compound [I-].[I-].[I-].[I-].C12=CC=CC=C2C(C=C2N(C3=CC=CC=C3S2)C)=CC=[N+]1CCC[N+](C)(C)CCC[N+](C)(C)CCC[N+](C1=CC=CC=C11)=CC=C1C=C1N(C)C2=CC=CC=C2S1 MZZINWWGSYUHGU-UHFFFAOYSA-J 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000607626 Vibrio cholerae Species 0.000 description 1
- GRRMZXFOOGQMFA-UHFFFAOYSA-J YoYo-1 Chemical compound [I-].[I-].[I-].[I-].C12=CC=CC=C2C(C=C2N(C3=CC=CC=C3O2)C)=CC=[N+]1CCC[N+](C)(C)CCC[N+](C)(C)CCC[N+](C1=CC=CC=C11)=CC=C1C=C1N(C)C2=CC=CC=C2O1 GRRMZXFOOGQMFA-UHFFFAOYSA-J 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000005018 aminopurines Chemical class 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000037429 base substitution Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 101150028015 cft1 gene Proteins 0.000 description 1
- WZUDOUFCWWUEPP-ZIYJDXEPSA-N chembl2018414 Chemical compound CC(=O)NC(C)(C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NC(C)(C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NC(C)(C)C(=O)N1[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CSC2C(N(CCCCCC(=O)NCCCCCCOP(O)(=O)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(NC(=O)C(C)=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C4=C(C(NC(N)=N4)=O)N=C3)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(NC(=O)C(C)=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(NC(=O)C(C)=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C4=C(C(NC(N)=N4)=O)N=C3)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C4=C(C(NC(N)=N4)=O)N=C3)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(NC(=O)C(C)=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)OP(O)(=S)OC[C@@H]3[C@H](C[C@@H](O3)N3C(N=C(N)C=C3)=O)O)C(=O)C2)=O)C(N)=O)CCC1 WZUDOUFCWWUEPP-ZIYJDXEPSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 125000006292 cyclic linker group Chemical group 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000005549 deoxyribonucleoside Substances 0.000 description 1
- 238000010511 deprotection reaction Methods 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- OKZIUSOJQLYFSE-UHFFFAOYSA-N difluoroboron Chemical compound F[B]F OKZIUSOJQLYFSE-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001046 green dye Substances 0.000 description 1
- 125000004051 hexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 101150020463 mft1 gene Proteins 0.000 description 1
- 101150014352 mtb12 gene Proteins 0.000 description 1
- HXTXFZYSIAHPTE-UHFFFAOYSA-N n-methoxy-7h-purin-6-amine Chemical compound CONC1=NC=NC2=C1NC=N2 HXTXFZYSIAHPTE-UHFFFAOYSA-N 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 239000002342 ribonucleoside Substances 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- QEMXHQIAXOOASZ-UHFFFAOYSA-N tetramethylammonium Chemical compound C[N+](C)(C)C QEMXHQIAXOOASZ-UHFFFAOYSA-N 0.000 description 1
- GJSGYPDDPQRWPK-UHFFFAOYSA-N tetrapentylammonium Chemical compound CCCCC[N+](CCCCC)(CCCCC)CCCCC GJSGYPDDPQRWPK-UHFFFAOYSA-N 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 229940118696 vibrio cholerae Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22472600P | 2000-08-11 | 2000-08-11 | |
| US60/224,726 | 2000-08-11 | ||
| US24061000P | 2000-10-16 | 2000-10-16 | |
| US60/240,610 | 2000-10-16 | ||
| PCT/US2001/025231 WO2002014555A2 (en) | 2000-08-11 | 2001-08-10 | Single-labeled oligonucleotide probes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2417986A1 CA2417986A1 (en) | 2002-02-21 |
| CA2417986C true CA2417986C (en) | 2013-11-26 |
Family
ID=26918967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2417986A Expired - Lifetime CA2417986C (en) | 2000-08-11 | 2001-08-10 | Single-labeled oligonucleotide probes |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6635427B2 (Direct) |
| EP (1) | EP1307592B1 (Direct) |
| JP (1) | JP5213297B2 (Direct) |
| AT (1) | ATE332398T1 (Direct) |
| CA (1) | CA2417986C (Direct) |
| DE (1) | DE60121342T2 (Direct) |
| ES (1) | ES2267803T3 (Direct) |
| WO (1) | WO2002014555A2 (Direct) |
Families Citing this family (94)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10027113A1 (de) * | 1999-12-23 | 2001-09-27 | Andreas Hoeft | Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens |
| FI20000333A0 (fi) * | 2000-02-16 | 2000-02-16 | Jussi Nurmi | Homogeeninen menetelmä polynukleotidin havaitsemiseksi |
| US7998673B2 (en) | 2000-03-29 | 2011-08-16 | Lgc Limited | Hybridisation beacon and method of rapid sequence detection and discrimination |
| EP1715063B1 (en) * | 2000-03-29 | 2011-01-26 | LGC Limited | Hybridisation beacon and method of rapid sequence detection and discrimination |
| AU2003281275A1 (en) | 2002-04-23 | 2004-01-23 | Idaho Technology, Inc. | Sample withdrawal and dispensing device |
| US7785776B2 (en) * | 2002-05-13 | 2010-08-31 | Idaho Technology, Inc. | Genotyping by amplicon melting curve analysis |
| JP2004024035A (ja) * | 2002-06-21 | 2004-01-29 | Tosoh Corp | 核酸の検出方法 |
| CA2433473A1 (en) * | 2002-07-23 | 2004-01-23 | F. Hoffmann-La Roche Ag | Fluorescent hybridization probes with reduced background |
| US7122384B2 (en) * | 2002-11-06 | 2006-10-17 | E. I. Du Pont De Nemours And Company | Resonant light scattering microparticle methods |
| EP1570079B1 (en) | 2002-12-06 | 2010-07-21 | Roche Diagnostics GmbH | Multiplex assay detection of pathogenic organisms |
| US7718361B2 (en) | 2002-12-06 | 2010-05-18 | Roche Molecular Systems, Inc. | Quantitative test for bacterial pathogens |
| ATE550099T1 (de) | 2003-04-04 | 2012-04-15 | Hoffmann La Roche | Verbessertes system für mehrfarbige echtzeit-pcr |
| JP4454249B2 (ja) | 2003-04-18 | 2010-04-21 | アークレイ株式会社 | 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット |
| US20050053950A1 (en) * | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
| EP1689764B1 (en) * | 2003-11-19 | 2013-01-02 | AlleLogic Biosciences Corporation | Oligonucleotides labeled with a plurality of fluorophores |
| FR2866349B1 (fr) * | 2004-02-12 | 2008-05-16 | Biomerieux Sa | Procede de diagnostic/pronostic d'une thrombose |
| WO2005098036A1 (en) * | 2004-04-12 | 2005-10-20 | Postech Foundation | Oligonucleotide for detecting target dna or rna |
| EP1634965B1 (en) | 2004-09-09 | 2010-01-20 | Roche Diagnostics GmbH | Real time PCR with the addition of pyrophosphatase |
| CA2594223C (en) * | 2005-01-03 | 2016-03-15 | The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Primer for nucleic acid detection |
| ES2284087T3 (es) | 2005-01-18 | 2007-11-01 | F. Hoffmann-La Roche Ag | Generacion de imagenes de señales de fluorescencia mediante opticas telecentricas de excitacion y de generacion de imagenes. |
| DE602005000877T2 (de) | 2005-01-18 | 2007-12-20 | Roche Diagnostics Gmbh | Fluoreszenzabbildung mittels Telezentrizität |
| US20060172324A1 (en) * | 2005-01-28 | 2006-08-03 | Roche Molecular Systems, Inc. | Methods of genotyping using differences in melting temperature |
| JP4398886B2 (ja) * | 2005-03-07 | 2010-01-13 | ソニー株式会社 | 通信端末装置、通信システム、通信方法、およびプログラム |
| EP1700922B1 (en) | 2005-03-10 | 2016-08-24 | Roche Diagnostics GmbH | 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them |
| US7759469B2 (en) | 2005-03-10 | 2010-07-20 | Roche Diagnostics Operations, Inc. | Labeling reagent |
| US7776567B2 (en) | 2005-03-17 | 2010-08-17 | Biotium, Inc. | Dimeric and trimeric nucleic acid dyes, and associated systems and methods |
| US7601498B2 (en) | 2005-03-17 | 2009-10-13 | Biotium, Inc. | Methods of using dyes in association with nucleic acid staining or detection and associated technology |
| GB0514889D0 (en) | 2005-07-20 | 2005-08-24 | Lgc Ltd | Oligonucleotides |
| US7781165B2 (en) | 2005-10-19 | 2010-08-24 | Roche Diagnostics Operations, Inc. | Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification |
| ATE443072T1 (de) | 2005-11-23 | 2009-10-15 | Roche Diagnostics Gmbh | Polynukleotid mit phosphatmimetikum |
| JP4713514B2 (ja) * | 2006-02-20 | 2011-06-29 | エフ．ホフマン−ラ ロシュ アーゲー | 改善された標識試薬 |
| CA2638854C (en) | 2006-02-27 | 2012-05-15 | F. Hoffmann-La Roche Ag | Pcr hot start by magnesium sequestration |
| RU2394915C2 (ru) * | 2006-03-24 | 2010-07-20 | Александр Борисович Четверин | Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления |
| WO2008020090A1 (es) * | 2006-08-18 | 2008-02-21 | Laboratorios Indas, S.A. | Método y kit para diagnosticar el riesgo de desarrollar una enfermedad trombótica venosa |
| BRPI0721095B1 (pt) | 2006-12-13 | 2015-09-29 | Luminex Corporation | Sistemas e métodos para a análise multíplex de pcr em tempo real |
| US8247171B2 (en) * | 2007-02-01 | 2012-08-21 | Abacus Diagnostica Oy | Method for detection of presence of target polynucleotide in samples |
| WO2009011297A1 (ja) * | 2007-07-13 | 2009-01-22 | Arkray, Inc. | Ｊａｋ２遺伝子の変異検出用プローブおよびその用途 |
| US20090181391A1 (en) * | 2007-11-01 | 2009-07-16 | Zymo Research Corporation | Methods for analysis of dna methylation percentage |
| CA2658520C (en) | 2008-03-19 | 2016-11-08 | F. Hoffmann-La Roche Ag | Nucleic acid amplification in the presence of modified randomers |
| EP2297358A2 (en) | 2008-05-30 | 2011-03-23 | Gen-Probe Incorporated | Compositions, kits and related methods for the detection and/or monitoring of salmonella |
| US9393566B2 (en) | 2008-06-23 | 2016-07-19 | Canon U.S. Life Sciences, Inc. | System and method for temperature referencing for melt curve data collection |
| EP2148187A1 (de) | 2008-07-25 | 2010-01-27 | Roche Diagnostics GmbH | Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion |
| WO2010018245A1 (es) * | 2008-08-12 | 2010-02-18 | Biotools Biotechnological & Medical Laboratories, S.A. | Método para la detección y/o cuantificación de un ácido nucleico substrato |
| US7910720B2 (en) | 2008-09-09 | 2011-03-22 | Roche Diagnostics Operations, Inc. | Polyanion for improved nucleic acid amplification |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| US9347092B2 (en) | 2009-02-25 | 2016-05-24 | Roche Molecular System, Inc. | Solid support for high-throughput nucleic acid analysis |
| EP2436777B1 (en) | 2009-05-26 | 2015-08-19 | Xiamen University | Method for detecting variations in nucleic acid sequences |
| WO2010147848A2 (en) | 2009-06-15 | 2010-12-23 | Rd Biosciences, Inc. | Kits and methods for selective amplification and detection of nucleic acid targets |
| US8748095B2 (en) * | 2009-10-30 | 2014-06-10 | Arkray, Inc. | Probe for detecting polymorphism in EGFR gene and use of the probe |
| CN102666852A (zh) * | 2009-11-19 | 2012-09-12 | 爱科来株式会社 | Mthfr基因扩增用引物对、含有其的mthfr基因扩增用试剂及其用途 |
| CN102317454A (zh) * | 2009-12-07 | 2012-01-11 | 爱科来株式会社 | 疾病相关基因的多态性检测用探针及其用途 |
| US8877437B1 (en) | 2009-12-23 | 2014-11-04 | Biotium, Inc. | Methods of using dyes in association with nucleic acid staining or detection |
| US9285319B2 (en) | 2010-04-21 | 2016-03-15 | Riken | Nucleic acid base analogs with quenching and fluorescent activities and applications thereof |
| JP5940771B2 (ja) | 2010-07-12 | 2016-06-29 | アークレイ株式会社 | ａｂｌ遺伝子多型の検出用プローブおよびその用途 |
| GB201017978D0 (en) | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
| US20120276533A1 (en) * | 2011-04-28 | 2012-11-01 | Arkray, Inc. | Method for Simultaneously Detecting Polymorphisms of Acetaldehyde Dehydrogenase 2 and Alcohol Dehydrogenase 2 |
| US10557170B2 (en) | 2011-06-16 | 2020-02-11 | Gendiag.Exe, S.L. | Thromboembolic disease markers |
| DK2535424T3 (en) * | 2011-06-16 | 2015-09-14 | Gendiag Exe Sl | SNPs associated with thromboembolic disease |
| EP2722388B1 (en) * | 2011-06-16 | 2017-08-09 | Nippon Steel & Sumikin Eco-Tech Corporation | Nucleic acid probe for assaying nucleic acids |
| US9366677B2 (en) * | 2011-09-06 | 2016-06-14 | Max-Planck-Gesellshaft Zur Foerderung Der Wissenschaften E.V. | Methods for analyzing biological macromolecular complexes and use thereof |
| JP2013074888A (ja) | 2011-09-15 | 2013-04-25 | Arkray Inc | ＩＬ２８Ｂ（ｒｓ８０９９９１７）とＩＴＰＡ（ｒｓ１１２７３５４）の変異を検出する方法 |
| WO2013112458A1 (en) | 2012-01-24 | 2013-08-01 | Beth Israel Deaconess Medical Center, Inc. | Novel chrebp isoforms and methods using the same |
| AU2013202808B2 (en) | 2012-07-31 | 2014-11-13 | Gen-Probe Incorporated | System and method for performing multiplex thermal melt analysis |
| EP4570924A3 (en) | 2013-01-24 | 2025-09-03 | California Institute of Technology | Chromophore-based characterization and detection methods |
| EP2999796B1 (en) | 2013-05-20 | 2017-10-25 | BioVentures, LLC | Gep5 model for multiple myeloma |
| GB201319180D0 (en) * | 2013-10-30 | 2013-12-11 | Mast Group Ltd | Nucleic acid probe |
| US10640833B2 (en) | 2014-06-26 | 2020-05-05 | University Of Florida Research Foundation, Inc. | Rapid detection of infectious agents |
| JP5813263B1 (ja) * | 2015-03-31 | 2015-11-17 | 日鉄住金環境株式会社 | 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド |
| CN114250274A (zh) | 2015-04-24 | 2022-03-29 | 阿提拉生物系统公司 | 利用有限核苷酸组成的引物扩增 |
| WO2017138484A1 (ja) | 2016-02-09 | 2017-08-17 | 栄研化学株式会社 | 標的核酸を検出する方法およびそれに用いる核酸プローブ |
| CN109804089A (zh) | 2016-07-29 | 2019-05-24 | 朱诺治疗学股份有限公司 | 用于评估复制型病毒存在或不存在的方法 |
| CN111050545A (zh) | 2017-06-29 | 2020-04-21 | 朱诺治疗学股份有限公司 | 评估与免疫疗法相关的毒性的小鼠模型 |
| KR20200064060A (ko) | 2017-07-29 | 2020-06-05 | 주노 쎄러퓨티크스 인코퍼레이티드 | 재조합 수용체를 발현하는 세포 증폭용 시약 |
| MA50057A (fr) | 2017-09-01 | 2020-07-08 | Juno Therapeutics Inc | Expression génique et évaluation d'un risque de développement d'une toxicité suite à une thérapie cellulaire |
| CN107727618B (zh) * | 2017-09-04 | 2019-12-31 | 江苏大学 | 生物芯片和特异性切割位点dna序列检测汞离子的方法 |
| US20210254000A1 (en) | 2017-11-01 | 2021-08-19 | Juno Therapeutics, Inc. | Process for producing a t cell composition |
| CN111918972A (zh) | 2018-01-31 | 2020-11-10 | 朱诺治疗学股份有限公司 | 评估存在或不存在有复制能力的病毒的方法和试剂 |
| GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
| CN113272018A (zh) | 2018-11-06 | 2021-08-17 | 朱诺治疗学股份有限公司 | 产生基因工程化t细胞的方法 |
| EP4438054A3 (en) | 2019-04-17 | 2025-01-08 | Alpine Immune Sciences, Inc. | Methods and uses of variant icos ligand (icosl) fusion proteins |
| KR20220034782A (ko) | 2019-06-12 | 2022-03-18 | 주노 쎄러퓨티크스 인코퍼레이티드 | 세포 매개 세포 독성 요법 및 친생존 ｂｃｌ2 패밀리 단백질 억제제의 병용 요법 |
| KR20220066892A (ko) | 2019-08-22 | 2022-05-24 | 주노 쎄러퓨티크스 인코퍼레이티드 | Ｔ 세포 요법 및 제스트 동족체 2의 인핸서 (ｅｚｈ2) 억제제의 병용 요법 및 관련 방법 |
| JP7697691B2 (ja) * | 2019-09-23 | 2025-06-24 | ユニベルシテイト ゲント | Ｓｔｒジェノタイピングのためのプローブおよび方法 |
| KR102107589B1 (ko) * | 2019-11-20 | 2020-05-07 | 주식회사 유진셀 | 단일 신호 형광 물질을 이용한 실시간 타겟 핵산 검출 및 정량 방법 |
| WO2021140173A1 (en) | 2020-01-10 | 2021-07-15 | Biouniversa S.R.L. | Methods and uses for treating fibrotic solid tumors with bags inhibitors |
| CN115335511A (zh) | 2020-01-13 | 2022-11-11 | 艾斯本神经科学公司 | 使神经细胞分化的方法及相关组合物和使用方法 |
| CN115835873A (zh) | 2020-05-13 | 2023-03-21 | 朱诺治疗学股份有限公司 | 用于产生表达重组受体的供体分批细胞的方法 |
| CN116234558A (zh) | 2020-06-26 | 2023-06-06 | 朱诺治疗学有限公司 | 条件性地表达重组受体的工程化t细胞、相关多核苷酸和方法 |
| WO2023004371A1 (en) | 2021-07-21 | 2023-01-26 | Aspen Neuroscience, Inc. | Methods of differentiating neural cells and predicting engraftment thereof and related compositions |
| AU2022402153A1 (en) | 2021-12-03 | 2024-06-13 | Medicinal Genomics Corporation | Psilocybe assay |
| CA3250467A1 (en) | 2022-04-15 | 2023-10-19 | Aspen Neuroscience, Inc. | METHODS FOR CLASSIFYING THE STATE OF DIFFERENTIATION OF CELLS AND ASSOCIATED COMPOSITIONS OF DIFFERENTIATED CELLS |
| EP4532695A1 (en) | 2022-05-25 | 2025-04-09 | Celgene Corporation | Methods of manufacturing t cell therapies |
| AU2023343062A1 (en) | 2022-09-13 | 2025-04-03 | Medicinal Genomics Corporation | Psilocybe assay |
| WO2025245399A1 (en) | 2024-05-23 | 2025-11-27 | Indapta Therapeutics, Inc. | Method of treating cancer with natural killer cells |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5597696A (en) * | 1994-07-18 | 1997-01-28 | Becton Dickinson And Company | Covalent cyanine dye oligonucleotide conjugates |
| DE69610304T2 (de) | 1995-05-05 | 2001-04-05 | Perkin-Elmer Corp., Foster City | Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay |
| GB9602025D0 (en) * | 1996-02-01 | 1996-04-03 | Amersham Int Plc | Nucleoside analogues |
| US5716784A (en) | 1996-02-05 | 1998-02-10 | The Perkin-Elmer Corporation | Fluorescence detection assay for homogeneous PCR hybridization systems |
| WO1997043451A1 (en) | 1996-05-15 | 1997-11-20 | Biogenex Laboratories | Non-nucleotide linking reagents |
| EP1033411B1 (en) | 1996-06-04 | 2006-02-22 | University of Utah Research Foundation | Fluorescent donor-acceptor pair |
| US5691145A (en) | 1996-08-27 | 1997-11-25 | Becton, Dickinson And Company | Detection of nucleic acids using G-quartets |
| US6451530B1 (en) * | 1996-12-13 | 2002-09-17 | The United States Of America As Represented By The Department Of Health And Human Services | Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization |
| SE522077C2 (sv) | 1997-09-05 | 2004-01-13 | Lightup Technologies Ab | Förfarande att välja sekvens hos sond för nukleinsyrahybridisering |
| US6465175B2 (en) * | 1997-09-04 | 2002-10-15 | Bayer Corporation | Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof |
| DE19858588B4 (de) | 1998-08-22 | 2016-04-07 | Qiagen Gmbh | Farbstoffmarkiertes Oligonukleotid zum Markieren eines Nukleinsäuremoleküls |
| CA2644688C (en) | 1999-04-20 | 2012-11-13 | Kankyo Engineering Co., Ltd. | Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analysing data obtained by the method |
| SE9902565D0 (sv) * | 1999-07-05 | 1999-07-05 | Jonas Karlsson | Probe for analysis of nucleic acids |
| EP1715063B1 (en) * | 2000-03-29 | 2011-01-26 | LGC Limited | Hybridisation beacon and method of rapid sequence detection and discrimination |
-
2001
- 2001-08-10 CA CA2417986A patent/CA2417986C/en not_active Expired - Lifetime
- 2001-08-10 EP EP01959722A patent/EP1307592B1/en not_active Expired - Lifetime
- 2001-08-10 ES ES01959722T patent/ES2267803T3/es not_active Expired - Lifetime
- 2001-08-10 AT AT01959722T patent/ATE332398T1/de not_active IP Right Cessation
- 2001-08-10 WO PCT/US2001/025231 patent/WO2002014555A2/en not_active Ceased
- 2001-08-10 US US09/927,842 patent/US6635427B2/en not_active Expired - Lifetime
- 2001-08-10 JP JP2002519680A patent/JP5213297B2/ja not_active Expired - Lifetime
- 2001-08-10 DE DE60121342T patent/DE60121342T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP5213297B2 (ja) | 2013-06-19 |
| WO2002014555A2 (en) | 2002-02-21 |
| US20030022177A1 (en) | 2003-01-30 |
| CA2417986A1 (en) | 2002-02-21 |
| JP2004506431A (ja) | 2004-03-04 |
| DE60121342T2 (de) | 2007-06-28 |
| EP1307592A2 (en) | 2003-05-07 |
| ES2267803T3 (es) | 2007-03-16 |
| WO2002014555A3 (en) | 2003-02-27 |
| ATE332398T1 (de) | 2006-07-15 |
| EP1307592B1 (en) | 2006-07-05 |
| DE60121342D1 (de) | 2006-08-17 |
| US6635427B2 (en) | 2003-10-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2417986C (en) | Single-labeled oligonucleotide probes | |
| KR102246600B1 (ko) | 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브 | |
| EP1362928B1 (en) | Genotyping by amplicon melting curve analysis | |
| CN101831496B (zh) | 多重定量核酸扩增及解链测定法 | |
| US20030134307A1 (en) | Asymmetric PCR with nuclease-free polymerase or nuclease-resistant molecular beacons | |
| US7504218B2 (en) | Key probe compositions and methods for polynucleotide detection | |
| CN106574304B (zh) | 基于链侵入的dna扩增方法 | |
| US20040259116A1 (en) | Hairpin primer amplification | |
| US20060194222A1 (en) | Triplex probe compositions and methods for polynucleotide detection | |
| Vaughn et al. | Hybridization-induced dequenching of fluorescein-labeled oligonucleotides: a novel strategy for PCR detection and genotyping | |
| AU2006244035A1 (en) | Snapback oligonucleotide probe | |
| CA2733653C (en) | Detection of target variants using a fluorescent label and a soluble quencher | |
| US9260746B2 (en) | Photoinduced electron transfer (PET) primer for nucleic acid amplification | |
| AU2002363464A1 (en) | Asymmetric PCR with nuclease-free polymerase or nuclease-resistant molecular beacons |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20210810 |