CA2396879A1 - Microbial 9a-hydroxylation of steroids - Google Patents

Microbial 9a-hydroxylation of steroids Download PDF

Info

Publication number
CA2396879A1
CA2396879A1 CA002396879A CA2396879A CA2396879A1 CA 2396879 A1 CA2396879 A1 CA 2396879A1 CA 002396879 A CA002396879 A CA 002396879A CA 2396879 A CA2396879 A CA 2396879A CA 2396879 A1 CA2396879 A1 CA 2396879A1
Authority
CA
Canada
Prior art keywords
gene
steroid
dione
androstene
kstd2
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002396879A
Other languages
English (en)
French (fr)
Inventor
Robert Van Der Geize
Gerda Hessels
Lubbert Dijkhuizen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Organon NV
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2396879A1 publication Critical patent/CA2396879A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002396879A 1999-10-22 2000-10-17 Microbial 9a-hydroxylation of steroids Abandoned CA2396879A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP99203470 1999-10-22
NL99203470.2 1999-10-22
NL99204449.5 1999-12-22
EP99204449 1999-12-22
PCT/EP2000/010223 WO2001031050A1 (en) 1999-10-22 2000-10-17 MICROBIAL 9α-HYDROXYLATION OF STEROIDS

Publications (1)

Publication Number Publication Date
CA2396879A1 true CA2396879A1 (en) 2001-05-03

Family

ID=26153382

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002396879A Abandoned CA2396879A1 (en) 1999-10-22 2000-10-17 Microbial 9a-hydroxylation of steroids

Country Status (11)

Country Link
EP (1) EP1232278A1 (cs)
CN (1) CN1224716C (cs)
AR (1) AR026186A1 (cs)
AU (1) AU775476B2 (cs)
CA (1) CA2396879A1 (cs)
CZ (1) CZ20021784A3 (cs)
IL (1) IL149715A0 (cs)
NO (1) NO20022449L (cs)
NZ (1) NZ519039A (cs)
RU (1) RU2268935C2 (cs)
WO (1) WO2001031050A1 (cs)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE465247T1 (de) 2002-02-21 2010-05-15 Organon Nv Identifikation von 3-ketosteroid 9-alfa- hydroxylase genen und blockierte mikroorganismen in 3-ketosteroid 9-alfa-hydroxylase aktivität
WO2004055189A1 (en) * 2002-12-03 2004-07-01 Akzo Nobel N.V. New expression system from rhodococcus
AT503486B1 (de) * 2006-04-11 2008-05-15 Iep Gmbh Verfahren zur enantioselektiven reduktion von steroiden
RU2351645C1 (ru) * 2007-07-20 2009-04-10 Наталия Евгеньевна Войшвилло ШТАММ Rhodococcus erythropolis ВКПМ Ac-1740 ДЛЯ ПОЛУЧЕНИЯ 9 АЛЬФА-ГИДРОКСИСТЕРОИДОВ
WO2009024572A1 (en) * 2007-08-21 2009-02-26 N.V. Organon Method for the production of modified steroid degrading microorganisms and their use
RU2011138954A (ru) 2009-02-23 2013-03-27 Рейксюниверситейт Гронинген Фармацевтическая композиция и способы лечения туберкулеза
CN103361394B (zh) * 2013-08-07 2016-08-17 中国科学院上海高等研究院 利用微生物转化制备9α-羟基-雄烯二酮的方法
CN103805577A (zh) * 2013-08-14 2014-05-21 济南环亿生物科技有限公司 一种采用睾丸酮丛毛单胞菌高效生产羟基类固醇脱氢酶的方法
WO2015128534A1 (es) 2014-02-27 2015-09-03 Consejo Superior De Investigaciones Científicas MUTANTES RECOMBINANTES SELECTIVOS DE MYCOBACTERIUM SMEGMATIS mc2 155 Y SU USO PARA LA PRODUCCIÓN DE 1,4-ANDROSTADIEN-3,17-DIONA O 4-ANDROSTEN-3,17-DIONA A PARTIR DE ESTEROLES NATURALES
CN107586762A (zh) * 2017-09-18 2018-01-16 天津科技大学 一种3‑甾酮‑δ1‑脱氢酶突变体及其应用
CN107955827B (zh) * 2017-12-15 2019-07-02 江南大学 一种酶法转化生产9α-羟基雄甾-4-烯-3,17-二酮的方法
US11001871B2 (en) 2017-12-15 2021-05-11 Jiangnan University Method for producing 9alpha-hydroxy androstane-4-alkene-3,17-diketone by enzymatic conversion
GB2577037A (en) * 2018-08-09 2020-03-18 Cambrex Karlskoga Ab Genetically-modified bacteria and uses thereof
AU2020219424A1 (en) * 2019-02-08 2021-08-26 Euroapi France Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids
CN110229838B (zh) * 2019-05-28 2021-01-08 浙江理工大学 一种生物转化类固醇类化合物获取羟基化化合物的方法
CN114621965B (zh) * 2022-02-15 2023-10-03 复旦大学 一种3-甾酮-δ1-脱氢酶突变体及应用
CN120366249A (zh) * 2025-04-23 2025-07-25 浙江工业大学 一种3-甾酮-δ1-脱氢酶突变体及在催化甾体化合物脱氢中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU196627B (en) * 1986-11-18 1988-12-28 Gyogyszerkutato Intezet Microbiological process for producing y alpha-hydroxy-4-androstene-3,17-dione
RU2039824C1 (ru) * 1993-06-22 1995-07-20 Институт биохимии и физиологии микроорганизмов РАН Способ получения андроста-1,4-диен-3,17-диона

Also Published As

Publication number Publication date
AR026186A1 (es) 2003-01-29
RU2002113373A (ru) 2004-02-27
CN1224716C (zh) 2005-10-26
CZ20021784A3 (cs) 2002-08-14
EP1232278A1 (en) 2002-08-21
IL149715A0 (en) 2002-11-10
NO20022449D0 (no) 2002-05-23
WO2001031050A1 (en) 2001-05-03
AU1514501A (en) 2001-05-08
AU775476B2 (en) 2004-08-05
CN1413260A (zh) 2003-04-23
NZ519039A (en) 2004-09-24
NO20022449L (no) 2002-06-19
RU2268935C2 (ru) 2006-01-27

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued