CA2270227C - Detection of antigens via oligonucleotide antibody conjugates - Google Patents

Detection of antigens via oligonucleotide antibody conjugates Download PDF

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Publication number
CA2270227C
CA2270227C CA002270227A CA2270227A CA2270227C CA 2270227 C CA2270227 C CA 2270227C CA 002270227 A CA002270227 A CA 002270227A CA 2270227 A CA2270227 A CA 2270227A CA 2270227 C CA2270227 C CA 2270227C
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CA
Canada
Prior art keywords
labeled
antibody
hybridization
polynucleotide
oligonucleotide
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002270227A
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English (en)
French (fr)
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CA2270227A1 (en
Inventor
Thor W. Nilsen
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Genisphere LLC
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Polyprobe Inc
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Publication of CA2270227A1 publication Critical patent/CA2270227A1/en
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Publication of CA2270227C publication Critical patent/CA2270227C/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002270227A 1996-10-29 1997-10-22 Detection of antigens via oligonucleotide antibody conjugates Expired - Fee Related CA2270227C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/738,641 1996-10-29
US08/738,641 US6117631A (en) 1996-10-29 1996-10-29 Detection of antigens via oligonucleotide antibody conjugates
PCT/US1997/019093 WO1998018488A1 (en) 1996-10-29 1997-10-22 Detection of antigens via oligonucleotide antibody conjugates

Publications (2)

Publication Number Publication Date
CA2270227A1 CA2270227A1 (en) 1998-05-07
CA2270227C true CA2270227C (en) 2005-02-08

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ID=24968855

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002270227A Expired - Fee Related CA2270227C (en) 1996-10-29 1997-10-22 Detection of antigens via oligonucleotide antibody conjugates

Country Status (9)

Country Link
US (2) US6117631A (https=)
EP (1) EP0951297B1 (https=)
JP (1) JP3786970B2 (https=)
AT (1) ATE320819T1 (https=)
AU (1) AU718804B2 (https=)
CA (1) CA2270227C (https=)
DE (1) DE69735532T2 (https=)
ES (1) ES2260787T3 (https=)
WO (1) WO1998018488A1 (https=)

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WO1998018488A1 (en) 1998-05-07
EP0951297B1 (en) 2006-03-22
DE69735532T2 (de) 2006-11-23
JP2001503517A (ja) 2001-03-13
ATE320819T1 (de) 2006-04-15
ES2260787T3 (es) 2006-11-01
US6110687A (en) 2000-08-29
DE69735532D1 (de) 2006-05-11
JP3786970B2 (ja) 2006-06-21
EP0951297A4 (en) 2002-01-02
CA2270227A1 (en) 1998-05-07
AU4914097A (en) 1998-05-22
AU718804B2 (en) 2000-04-20
US6117631A (en) 2000-09-12
EP0951297A1 (en) 1999-10-27

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