CA2233042C - Specific binding members for human transforming growth factor beta; materials and methods - Google Patents
Specific binding members for human transforming growth factor beta; materials and methods Download PDFInfo
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- CA2233042C CA2233042C CA002233042A CA2233042A CA2233042C CA 2233042 C CA2233042 C CA 2233042C CA 002233042 A CA002233042 A CA 002233042A CA 2233042 A CA2233042 A CA 2233042A CA 2233042 C CA2233042 C CA 2233042C
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
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- C—CHEMISTRY; METALLURGY
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- C07K—PEPTIDES
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- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
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Landscapes
- Health & Medical Sciences (AREA)
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- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002599488A CA2599488A1 (en) | 1995-10-06 | 1996-10-07 | Specific binding members for human transforming growth factor beta; materials and methods |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9520486.3A GB9520486D0 (en) | 1995-10-06 | 1995-10-06 | Specific binding members for human transforming growth factor beta; materials and methods |
| GB9520486.3 | 1996-01-19 | ||
| GBGB9601081.4A GB9601081D0 (en) | 1995-10-06 | 1996-01-19 | Specific binding members for human transforming growth factor beta;materials and methods |
| GB9601081.4 | 1996-01-19 | ||
| PCT/GB1996/002450 WO1997013844A1 (en) | 1995-10-06 | 1996-10-07 | Specific binding members for human transforming growth factor beta; materials and methods |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002599488A Division CA2599488A1 (en) | 1995-10-06 | 1996-10-07 | Specific binding members for human transforming growth factor beta; materials and methods |
Publications (2)
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| CA2233042A1 CA2233042A1 (en) | 1997-04-17 |
| CA2233042C true CA2233042C (en) | 2007-12-18 |
Family
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
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| CA002233042A Expired - Fee Related CA2233042C (en) | 1995-10-06 | 1996-10-07 | Specific binding members for human transforming growth factor beta; materials and methods |
| CA002599488A Abandoned CA2599488A1 (en) | 1995-10-06 | 1996-10-07 | Specific binding members for human transforming growth factor beta; materials and methods |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002599488A Abandoned CA2599488A1 (en) | 1995-10-06 | 1996-10-07 | Specific binding members for human transforming growth factor beta; materials and methods |
Country Status (12)
| Country | Link |
|---|---|
| EP (2) | EP0853661B1 (enExample) |
| JP (1) | JP4387458B2 (enExample) |
| AT (2) | ATE199091T1 (enExample) |
| AU (1) | AU702049B2 (enExample) |
| CA (2) | CA2233042C (enExample) |
| DE (2) | DE69611766T2 (enExample) |
| DK (2) | DK0945464T3 (enExample) |
| ES (2) | ES2146020T3 (enExample) |
| GB (2) | GB9601081D0 (enExample) |
| GR (2) | GR3033436T3 (enExample) |
| PT (2) | PT945464E (enExample) |
| WO (1) | WO1997013844A1 (enExample) |
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| US6025154A (en) | 1995-06-06 | 2000-02-15 | Human Genome Sciences, Inc. | Polynucleotides encoding human G-protein chemokine receptor HDGNR10 |
| US7368111B2 (en) | 1995-10-06 | 2008-05-06 | Cambridge Antibody Technology Limited | Human antibodies specific for TGFβ2 |
| US7888466B2 (en) | 1996-01-11 | 2011-02-15 | Human Genome Sciences, Inc. | Human G-protein chemokine receptor HSATU68 |
| WO2000001410A1 (en) | 1998-07-06 | 2000-01-13 | Beth Israel Deaconess Medical Center | Methods of inhibiting proliferative diseases by inhibiting tgf-beta mediated angiogenesis |
| EP1166112A4 (en) * | 1999-04-09 | 2004-11-10 | Hanmi Pharm Ind Co Ltd | METHOD FOR QUANTIFYING BETA-1 TRANSFORMING GROWTH FACTOR AND METHOD FOR DETECTING CANCER USING THE SAME |
| US6492497B1 (en) | 1999-04-30 | 2002-12-10 | Cambridge Antibody Technology Limited | Specific binding members for TGFbeta1 |
| EP1486560A3 (en) * | 1999-04-30 | 2005-02-09 | Cambridge Antibody Technology LTD | Specific antibodies and antibody fragments for TGFBETA1 |
| US20040001826A1 (en) | 1999-06-30 | 2004-01-01 | Millennium Pharmaceuticals, Inc. | Glycoprotein VI and uses thereof |
| US7291714B1 (en) | 1999-06-30 | 2007-11-06 | Millennium Pharmaceuticals, Inc. | Glycoprotein VI and uses thereof |
| AU7825500A (en) * | 1999-10-07 | 2001-04-23 | Pharmacia Corporation | Inhibition enzyme linked immunosorbent assay (iELISA) for the detection and quantification of antibodies in biological samples |
| SE9903895D0 (sv) | 1999-10-28 | 1999-10-28 | Active Biotech Ab | Novel compounds |
| EP2281842A1 (en) | 2000-06-16 | 2011-02-09 | Human Genome Sciences, Inc. | Antibodies that immunospecifically bind to BLyS |
| EP1345625B1 (en) | 2000-11-28 | 2013-07-17 | MedImmune, LLC | Methods of administering/dosing anti-rsv antibodies for prophylaxis and treatment |
| WO2002044379A2 (en) * | 2000-11-28 | 2002-06-06 | Amgen, Inc. | Transforming growth factor-beta-related molecules and uses thereof |
| US7175988B2 (en) | 2001-02-09 | 2007-02-13 | Human Genome Sciences, Inc. | Human G-protein Chemokine Receptor (CCR5) HDGNR10 |
| WO2002064612A2 (en) * | 2001-02-09 | 2002-08-22 | Human Genome Sciences, Inc. | Human g-protein chemokine receptor (ccr5) hdgnr10 |
| AU2002257142B2 (en) | 2001-04-13 | 2008-09-04 | Human Genome Sciences, Inc. | Vascular endothelial growth factor 2 |
| PT1572874E (pt) | 2001-05-25 | 2013-12-11 | Human Genome Sciences Inc | Anticorpos que se ligam imunoespecificamente aos receptores de trail |
| US7393934B2 (en) | 2001-12-21 | 2008-07-01 | Human Genome Sciences, Inc. | Human G-protein chemokine receptor (CCR5) HDGNR10 |
| WO2003104428A2 (en) | 2002-01-21 | 2003-12-18 | Vaccinex, Inc. | Gene differentially expressed in breast and bladder cancer and encoded polypeptides |
| DE10210427A1 (de) | 2002-03-09 | 2003-10-09 | Hans Konrad Mueller-Hermelink | Humaner monoklonaler Antikörper |
| WO2003086458A1 (en) | 2002-04-12 | 2003-10-23 | Medimmune, Inc. | Recombinant anti-interleukin-9 antibodies |
| US7425618B2 (en) | 2002-06-14 | 2008-09-16 | Medimmune, Inc. | Stabilized anti-respiratory syncytial virus (RSV) antibody formulations |
| US7772372B2 (en) | 2002-07-04 | 2010-08-10 | Patrys Limited | Neoplasm specific antibodies and uses thereof |
| SI1551876T1 (sl) | 2002-10-16 | 2011-07-29 | Purdue Pharma Lp | Protitelesa, ki se vežejo na celično povezan CA 125/0722P, in postopki za njihovo uporabo |
| GB0224436D0 (en) * | 2002-10-21 | 2002-11-27 | Univ Cambridge Tech | Polypetides methods and means |
| BR0316880A (pt) | 2002-12-23 | 2005-10-25 | Wyeth Corp | Anticorpos contra pd-1 e usos dos mesmos |
| DE10311248A1 (de) | 2003-03-14 | 2004-09-30 | Müller-Hermelink, Hans Konrad, Prof. Dr. | Humaner monoklonaler Antikörper |
| CA2521826C (en) | 2003-04-11 | 2013-08-06 | Jennifer L. Reed | Recombinant il-9 antibodies and uses thereof |
| JP2007525188A (ja) | 2003-05-16 | 2007-09-06 | インターミューン インコーポレイテッド | 合成ケモカイン受容体リガンドおよびその使用方法 |
| EP1646655A2 (en) * | 2003-07-09 | 2006-04-19 | Eli Lilly And Company | Tgf-beta1 ligands |
| EP1644408A1 (en) | 2003-07-15 | 2006-04-12 | Barros Research Institute | Eimeria tenella antigen for immunotherapy of coccidiosis |
| EP1648935A2 (en) | 2003-07-25 | 2006-04-26 | Amgen Inc. | Antagonists and agonists of ldcam and methods of use |
| AU2004263896A1 (en) | 2003-08-08 | 2005-02-17 | Genenews Inc. | Osteoarthritis biomarkers and uses thereof |
| EP1531162A1 (en) | 2003-11-14 | 2005-05-18 | Heinz Vollmers | Adenocarcinoma specific antibody SAM-6, and uses thereof |
| DE10353175A1 (de) | 2003-11-14 | 2005-06-16 | Müller-Hermelink, Hans Konrad, Prof. Dr. | Humaner monoklonaler Antikörper mit fettsenkender Wirkung |
| WO2005105841A2 (en) | 2004-03-12 | 2005-11-10 | Human Genome Sciences, Inc. | Human g-protein chemokine receptor (ccr5) hdgnr10 |
| AU2005230848B9 (en) | 2004-03-31 | 2011-06-02 | Genentech, Inc. | Humanized anti-TGF-beta antibodies |
| US7597884B2 (en) | 2004-08-09 | 2009-10-06 | Alios Biopharma, Inc. | Hyperglycosylated polypeptide variants and methods of use |
| EP3073267A1 (en) | 2004-09-21 | 2016-09-28 | Medimmune, Inc. | Antibodies against and methods for producing vaccines for respiratory syncytial virus |
| JP2008518023A (ja) | 2004-10-27 | 2008-05-29 | メディミューン,インコーポレーテッド | 同族抗原に対する親和性を改変することによる抗体特異性の調節 |
| CA2599589A1 (en) | 2005-02-07 | 2006-08-17 | Genenews,Inc. | Mild osteoarthritis biomarkers and uses thereof |
| MX2007009545A (es) | 2005-02-08 | 2008-03-11 | Genzyme Corp | Anticuerpos para tgfbeta. |
| AU2006214121B9 (en) | 2005-02-15 | 2013-02-14 | Duke University | Anti-CD19 antibodies and uses in oncology |
| JP2008531730A (ja) | 2005-03-04 | 2008-08-14 | キュアーディーエム、インク. | I型真性糖尿病及び他の症状を治療するための方法及び薬学的組成物 |
| ES2361269T3 (es) | 2005-04-22 | 2011-06-15 | Eli Lilly And Company | Anticuerpos especificos de tgf beta 1. |
| AU2006244445B2 (en) | 2005-05-05 | 2013-04-18 | Duke University | Anti-CD19 antibody therapy for autoimmune disease |
| US7393919B2 (en) | 2005-05-25 | 2008-07-01 | Cure Dm, Inc. | Peptides, derivatives and analogs thereof, and methods of using same |
| EP1893647A2 (en) | 2005-06-23 | 2008-03-05 | MedImmune, Inc. | Antibody formulations having optimized aggregation and fragmentation profiles |
| EP1945816B1 (en) | 2005-10-21 | 2011-07-27 | GeneNews Inc. | Method and apparatus for correlating levels of biomarker products with disease |
| WO2007050793A2 (en) | 2005-10-25 | 2007-05-03 | The Johns Hopkins University | Methods and compositions for the treatment of marfan syndrome and associated disorders |
| WO2007103469A2 (en) | 2006-03-06 | 2007-09-13 | Aeres Biomedical Ltd. | Humanized anti-cd22 antibodies and their use in treatment of oncology, transplantation and autoimmune disease |
| EP2230252A1 (en) | 2006-03-13 | 2010-09-22 | The Johns Hopkins University | Augmentation of endothelial thromboresistance |
| AU2007260687B2 (en) | 2006-06-14 | 2013-12-12 | Provention Bio, Inc. | Methods for the treatment of autoimmune disorders using monoclonal antibodies with reduced toxicity |
| ES2439994T3 (es) | 2006-08-28 | 2014-01-27 | Kyowa Hakko Kirin Co., Ltd. | Anticuerpos antagonistas monoclonales humanos específicos de LIGHT humano |
| EP2918288B1 (en) | 2006-10-03 | 2017-08-16 | Genzyme Corporation | Use of TGF beta antagonists to treat infants at risk of developing bronchopulmonary dysplasia |
| US8785400B2 (en) | 2006-11-22 | 2014-07-22 | Curedm Group Holdings, Llc | Methods and compositions relating to islet cell neogenesis |
| EP2687232A1 (en) | 2006-12-06 | 2014-01-22 | MedImmune, LLC | Methods of treating systemic lupus erythematosus |
| CN101678103A (zh) | 2007-03-30 | 2010-03-24 | 米迪缪尼股份有限公司 | 抗体制剂 |
| BRPI0811466A2 (pt) | 2007-05-07 | 2014-10-14 | Medimmune Llc | Anticorpo anti-icos isolado, ácido nucleico, vetor, célula isolada, métodos para produzir um anticorpo, para tratar uma doença ou distúrbio, para tratar ou prevenir a rejeição em um paciente de transplante humano, para tratar uma malignidade de célula t em um ser humano, para esgotar células t que expressam icos em um paciente humano, para romper a arquitetura do centro germinal em um órgão linfóide secundário de um primata, para esgotar células b centrais germinais de órgão linfóide secundário de um primata, e para esgotar células b comutadas em classes circulantes em um primata, e, composição farmacêutica. |
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| US9308257B2 (en) | 2007-11-28 | 2016-04-12 | Medimmune, Llc | Protein formulation |
| JP2011509675A (ja) | 2008-01-18 | 2011-03-31 | メディミューン,エルエルシー | 部位特異的コンジュゲーションのためのシステイン操作抗体 |
| PL2250279T3 (pl) | 2008-02-08 | 2016-11-30 | Przeciwciała anty-ifnar1 o zmniejszonym powinowactwie do liganda fc | |
| CN103408663B (zh) | 2008-10-31 | 2016-01-27 | 东丽株式会社 | 抗人cxcl1单克隆抗体或其片段 |
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| EP2387584A1 (en) | 2009-01-14 | 2011-11-23 | IQ Therapeutics BV | Combination antibodies for the treatment and prevention of disease caused by bacillus anthracis and related bacteria and their toxins |
| US8852608B2 (en) | 2009-02-02 | 2014-10-07 | Medimmune, Llc | Antibodies against and methods for producing vaccines for respiratory syncytial virus |
| EP2711018A1 (en) | 2009-06-22 | 2014-03-26 | MedImmune, LLC | Engineered Fc regions for site-specific conjugation |
| KR101778317B1 (ko) | 2009-08-13 | 2017-09-13 | 얀센 백신스 앤드 프리벤션 비.브이. | 사람 호흡기 세포융합 바이러스(rsv)에 대한 항체 및 이용 방법 |
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| EP2737083A1 (en) | 2011-07-27 | 2014-06-04 | INSERM (Institut National de la Santé et de la Recherche Scientifique) | Methods for diagnosing and treating myhre syndrome |
| US9468612B2 (en) | 2011-10-26 | 2016-10-18 | Seattle Children's Hospital | Cysteamine in the treatment of fibrotic disease |
| WO2013078286A1 (en) | 2011-11-22 | 2013-05-30 | Cornell University | Methods for stimulating hematopoietic recovery by inhibiting tgf beta signaling |
| CA3111357A1 (en) | 2011-12-23 | 2013-06-27 | Pfizer Inc. | Engineered antibody constant regions for site-specific conjugation and methods and uses therefor |
| EP2799537B1 (en) | 2011-12-28 | 2021-09-22 | Kyoto Prefectural Public University Corporation | Normalization of culture of corneal endothelial cells |
| WO2014055442A2 (en) | 2012-10-01 | 2014-04-10 | The Trustees Of The University Of Pennsylvania | Compositions and methods for targeting stromal cells for the treatment of cancer |
| DK2953634T3 (da) | 2013-02-07 | 2021-08-30 | Massachusetts Gen Hospital | Fremgangsmåder til udvidelse eller udtømning af regulerende t-celler |
| WO2014129895A1 (en) | 2013-02-19 | 2014-08-28 | Stichting Vu-Vumc | Means and method for increasing the sensitivity of cancers for radiotherapy |
| EP2971048B1 (en) | 2013-03-11 | 2018-10-31 | Genzyme Corporation | Engineered anti-tgf-beta antibodies and antigen-binding fragments |
| LT2970890T (lt) | 2013-03-14 | 2020-07-10 | The Brigham And Women`S Hospital, Inc. | Kompozicijos ir būdai, skirti epitelinių kamieninių ląstelių padauginimui ir kultivavimui |
| SG11201509982UA (enExample) | 2013-06-06 | 2016-04-28 | Igenica Biotherapeutics Inc | |
| CA2914924A1 (en) | 2013-06-13 | 2014-12-18 | Fast Forward Pharmaceuticals B.V. | Cd40 signalling inhibitor and a further compound, wherein the further compound is a bile acid, a bile acid derivative, an tgr5-receptor agonist, an fxr agonist or a combination thereof, for the treatment of chronic inflammation, and the prevention of gastrointestinal cancer or fibrosis. |
| AU2014332442B2 (en) | 2013-08-26 | 2019-12-19 | Biontech Research And Development, Inc. | Nucleic acids encoding human antibodies to Sialyl-Lewis |
| US20160245815A1 (en) | 2013-10-01 | 2016-08-25 | Toray Industries, Inc. | Method for detecting pancreatic tumor, antibodies, and kit for the detection of pancreatic tumor |
| PL3064222T3 (pl) | 2013-10-31 | 2021-04-19 | Kyoto Prefectural Public University Corporation | Lek terapeutyczny zawierający inhibitor sygnału tgf-beta w chorobach związanych z retikulum endoplazmatycznym i śmiercią komórki w śródbłonku rogówki |
| GB201403775D0 (en) | 2014-03-04 | 2014-04-16 | Kymab Ltd | Antibodies, uses & methods |
| HUE053857T2 (hu) | 2014-05-28 | 2021-07-28 | Agenus Inc | Anti-GITR antitestek és eljárások azok elõállítására |
| KR102614642B1 (ko) | 2014-06-04 | 2023-12-19 | 바이오엔테크 리서치 앤드 디벨롭먼트 인코포레이티드 | 강글리오사이드 gd2에 대한 사람 단클론 항체 |
| KR102493376B1 (ko) | 2014-09-03 | 2023-01-27 | 더 브리검 앤드 우먼즈 하스피털, 인크. | 청력 손실의 치료를 위해 내이 털세포를 생성하기 위한 조성물, 시스템, 및 방법 |
| CN113956361B (zh) | 2014-10-01 | 2025-06-17 | 免疫医疗有限公司 | 替格瑞洛的抗体及其使用方法 |
| ES2836550T3 (es) | 2014-12-11 | 2021-06-25 | Pf Medicament | Anticuerpos anti-C10orf54 y utilizaciones de los mismos |
| CA2972048C (en) | 2014-12-22 | 2023-03-07 | The Rockefeller University | Anti-mertk agonistic antibodies and uses thereof |
| US11231423B2 (en) | 2015-01-26 | 2022-01-25 | Toray Industries, Inc. | Method and kit for the detection of biliary tract cancer |
| DK3267197T3 (da) | 2015-03-02 | 2020-10-12 | Toray Industries | Fremgangsmåde og kit til detektionen af pankreatisk dysfunktion |
| EP4137157A1 (en) | 2015-03-03 | 2023-02-22 | Kymab Limited | Antibodies, uses and methods |
| AU2016256911B2 (en) | 2015-05-07 | 2022-03-31 | Agenus Inc. | Anti-OX40 antibodies and methods of use thereof |
| CN107849142B (zh) | 2015-05-15 | 2022-04-26 | 综合医院公司 | 拮抗性抗肿瘤坏死因子受体超家族抗体 |
| WO2016187312A1 (en) | 2015-05-18 | 2016-11-24 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | Methods and compositions for treating an alphavirus infection |
| PT3303394T (pt) | 2015-05-29 | 2020-07-01 | Ludwig Inst For Cancer Res Ltd | Anticorpos anti-ctla-4 e métodos de uso dos mesmos |
| EP3340999A4 (en) | 2015-08-28 | 2019-06-12 | The General Hospital Corporation | AGONISTIC ANTI-TUMOR NECROSIS RECEPTOR 2 ANTIBODIES |
| CN107949573B (zh) | 2015-09-01 | 2022-05-03 | 艾吉纳斯公司 | 抗-pd-1抗体及其使用方法 |
| US11021687B2 (en) | 2016-01-08 | 2021-06-01 | The Brigham And Women's Hospital, Inc. | Production of differentiated enteroendocrine cells and insulin producing cells |
| AU2017219254B2 (en) * | 2016-02-17 | 2019-12-12 | Novartis Ag | TGFbeta 2 antibodies |
| US10213511B2 (en) | 2016-03-02 | 2019-02-26 | Frequency Therapeutics, Inc. | Thermoreversible compositions for administration of therapeutic agents |
| US10201540B2 (en) | 2016-03-02 | 2019-02-12 | Frequency Therapeutics, Inc. | Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using GSK3 inhibitors: I |
| US11260130B2 (en) | 2016-03-02 | 2022-03-01 | Frequency Therapeutics, Inc. | Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using a GSK3 inhibitor: IV |
| KR20180122397A (ko) * | 2016-03-11 | 2018-11-12 | 스칼러 락, 인크. | TGFβ1-결합 이뮤노글로불린 및 그의 용도 |
| KR102410778B1 (ko) | 2016-05-13 | 2022-06-21 | 더 제너럴 하스피탈 코포레이션 | 길항성 항-종양 괴사 인자 수용체 슈퍼패밀리 항체 |
| KR20190007026A (ko) | 2016-05-17 | 2019-01-21 | 제넨테크, 인크. | 면역요법에서의 진단 및 사용을 위한 기질 유전자 특질 |
| MY195089A (en) | 2016-05-27 | 2023-01-10 | Agenus Inc | Anti-Tim-3 Antibodies and Methods of use thereof |
| KR20190039937A (ko) | 2016-07-08 | 2019-04-16 | 스태튼 바이오테크놀로지 비.브이. | 항-ApoC3 항체 및 이의 사용 방법 |
| KR20230133934A (ko) | 2016-10-11 | 2023-09-19 | 아게누스 인코포레이티드 | 항-lag-3 항체 및 이의 사용 방법 |
| US11779604B2 (en) | 2016-11-03 | 2023-10-10 | Kymab Limited | Antibodies, combinations comprising antibodies, biomarkers, uses and methods |
| CA3042989A1 (en) | 2016-11-07 | 2018-05-11 | Junho Chung | Anti-family with sequence similarity 19, member a5 antibodies and method of use thereof |
| MA50949B1 (fr) | 2016-12-07 | 2023-12-29 | Memorial Sloan Kettering Cancer Center | Anticorps anti-ctla-4 et leurs procédés d'utilisation |
| MA50948A (fr) | 2016-12-07 | 2020-10-14 | Agenus Inc | Anticorps et procédés d'utilisation de ceux-ci |
| US11066419B2 (en) | 2016-12-30 | 2021-07-20 | Frequency Therapeutics, Inc. | 1H-pyrrole-2,5-dione compounds and methods of using same |
| TWI856437B (zh) | 2017-01-20 | 2024-09-21 | 法商賽諾菲公司 | 抗TGF-β抗體及其用途 |
| AR110755A1 (es) | 2017-01-20 | 2019-05-02 | Genzyme Corp | Anticuerpos dirigidos a hueso |
| JP7297672B2 (ja) | 2017-04-13 | 2023-06-26 | アジェナス インコーポレイテッド | 抗cd137抗体およびその使用方法 |
| WO2018193427A1 (en) | 2017-04-21 | 2018-10-25 | Staten Biotechnology B.V. | Anti-apoc3 antibodies and methods of use thereof |
| TW202402800A (zh) | 2017-05-01 | 2024-01-16 | 美商艾吉納斯公司 | 抗tigit抗體類和使用彼等之方法 |
| JOP20190256A1 (ar) | 2017-05-12 | 2019-10-28 | Icahn School Med Mount Sinai | فيروسات داء نيوكاسل واستخداماتها |
| BR112019027729A2 (pt) | 2017-06-27 | 2020-08-18 | Neuracle Science Co., Ltd | anticorpos anti-fam19a5 e seus usos |
| MX2020002076A (es) | 2017-08-25 | 2020-03-24 | Five Prime Therapeutics Inc | Anticuerpos de b7-h4 y metodos para usarlos. |
| EP3694889A1 (en) | 2017-10-13 | 2020-08-19 | Boehringer Ingelheim International GmbH | Human antibodies to thomsen-nouvelle (tn) antigen |
| SG11202003980PA (en) | 2017-10-31 | 2020-05-28 | Staten Biotechnology B V | Anti-apoc3 antibodies and methods of use thereof |
| CN111213059B (zh) | 2017-11-06 | 2024-01-09 | 豪夫迈·罗氏有限公司 | 用于癌症的诊断和治疗方法 |
| MX2020005473A (es) | 2017-11-27 | 2020-08-27 | Purdue Pharma Lp | Anticuerpos humanizados que se dirigen al factor tisular humano. |
| CN111741978A (zh) | 2018-02-21 | 2020-10-02 | 戊瑞治疗有限公司 | B7-h4抗体制剂 |
| CN111971308A (zh) | 2018-03-02 | 2020-11-20 | 戊瑞治疗有限公司 | B7-h4抗体及其使用方法 |
| WO2019215702A1 (en) | 2018-05-10 | 2019-11-14 | Neuracle Science Co., Ltd. | Anti-family with sequence similarity 19, member a5 antibodies and method of use thereof |
| US12006357B2 (en) | 2018-06-26 | 2024-06-11 | Mor Research Applications Ltd. | Transthyretin antibodies and uses thereof |
| EP3824287A1 (en) | 2018-07-20 | 2021-05-26 | Pierre Fabre Médicament | Receptor for vista |
| CN113195707A (zh) | 2018-08-17 | 2021-07-30 | 频率治疗公司 | 用于通过上调jag-1来生成毛细胞的组合物和方法 |
| WO2020037326A1 (en) | 2018-08-17 | 2020-02-20 | Frequency Therapeutics, Inc. | Compositions and methods for generating hair cells by downregulating foxo |
| EP3837277A4 (en) | 2018-08-17 | 2022-05-11 | Icahn School of Medicine at Mount Sinai | RECOMBINANT NEWCASTLE DISEASE VIRUSES AND THEIR USES IN THE PREVENTION OF RSV DISEASE OR HUMAN METAPNEUMOVIRUS INFECTION |
| TW202019958A (zh) | 2018-09-28 | 2020-06-01 | 日商協和麒麟股份有限公司 | Il-36抗體及其用途 |
| JP2022504287A (ja) | 2018-10-03 | 2022-01-13 | スターテン・バイオテクノロジー・ベー・フェー | ヒト及びカニクイザルapoc3に特異的な抗体、並びにその使用の方法 |
| JP7652690B2 (ja) | 2018-11-15 | 2025-03-27 | ザ ジェネラル ホスピタル コーポレイション | アゴニスト性腫瘍壊死因子受容体スーパーファミリーポリペプチド |
| US12234292B2 (en) | 2018-12-20 | 2025-02-25 | Kyowa Kirin Co., Ltd. | FN14 antibodies and uses thereof |
| KR102656738B1 (ko) | 2019-01-02 | 2024-04-16 | 주식회사 뉴라클사이언스 | 항-서열 유사성 19를 가진 패밀리, 멤버 a5 항체 및 이의 사용 방법 |
| US11242407B2 (en) | 2019-02-26 | 2022-02-08 | Inspirna, Inc. | High-affinity anti-MERTK antibodies and uses thereof |
| CN113874392B (zh) | 2019-03-28 | 2025-10-21 | 丹尼斯科美国公司 | 工程化抗体 |
| EP3947737A2 (en) | 2019-04-02 | 2022-02-09 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods of predicting and preventing cancer in patients having premalignant lesions |
| JP7691138B2 (ja) | 2019-08-12 | 2025-06-11 | アプティーボ リサーチ アンド デベロップメント エルエルシー | 4-1bbおよび0x40結合タンパク質ならびに関連する組成物および方法、4-1bbに対する抗体、0x40に対する抗体 |
| PE20221151A1 (es) | 2019-08-30 | 2022-07-18 | Agenus Inc | Anticuerpos anti-cd96 y sus metodos de uso |
| WO2021044014A1 (en) | 2019-09-04 | 2021-03-11 | Y-Biologics Inc. | Anti-vsig4 antibody or antigen binding fragment and uses thereof |
| CN112852738B (zh) * | 2019-11-27 | 2023-02-28 | 四川省肿瘤医院 | 一种前列腺癌细胞膜片的制备方法 |
| CA3166155A1 (en) | 2020-02-18 | 2021-08-26 | Spencer LIANG | Pilra antibodies and methods of use thereof |
| AU2021232158A1 (en) | 2020-03-06 | 2022-09-29 | Ona Therapeutics, S.L. | Anti-CD36 antibodies and their use to treat cancer |
| EP4356924A3 (en) | 2020-03-26 | 2024-06-12 | Vanderbilt University | Human monoclonal antibodies to severe acute respiratory syndrome coronavirus 2 (sars-cov-2) |
| WO2021202473A2 (en) | 2020-03-30 | 2021-10-07 | Danisco Us Inc | Engineered antibodies |
| JP2023528235A (ja) | 2020-05-17 | 2023-07-04 | アストラゼネカ・ユーケイ・リミテッド | SARS-CoV-2抗体、並びにこれを選択及び使用する方法 |
| KR20230038783A (ko) | 2020-07-20 | 2023-03-21 | 아스트라제네카 유케이 리미티드 | Sars-cov-2 단백질, 항-sars-cov-2 항체, 및 이를 사용하는 방법 |
| SI4200018T1 (sl) | 2020-08-18 | 2025-07-31 | Cephalon Llc | Protitelesa proti-PAR-2 in postopki njihove uporabe |
| EP4229081A1 (en) | 2020-10-15 | 2023-08-23 | The United States of America, as represented by The Secretary, Department of Health and Human Services | Antibody specific for sars-cov-2 receptor binding domain and therapeutic methods |
| WO2022087274A1 (en) | 2020-10-21 | 2022-04-28 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Antibodies that neutralize type-i interferon (ifn) activity |
| EP4255574A1 (en) | 2020-12-01 | 2023-10-11 | Aptevo Research and Development LLC | Heterodimeric psma and cd3-binding bispecific antibodies |
| WO2022184853A1 (en) | 2021-03-03 | 2022-09-09 | Pierre Fabre Medicament | Anti-vsig4 antibody or antigen binding fragment and uses thereof |
| CA3214633A1 (en) | 2021-03-24 | 2022-09-29 | Toray Industries, Inc. | Method and kit for assisting in determination of malignant pancreatic cystic tumor |
| CA3220629A1 (en) | 2021-05-28 | 2022-12-01 | Tobin J. CAMMETT | Methods for detecting cm-tma biomarkers |
| IL309349A (en) | 2021-06-14 | 2024-02-01 | argenx BV | Antibodies against interleukin 9 and methods of using them |
| WO2023007472A1 (en) | 2021-07-30 | 2023-02-02 | ONA Therapeutics S.L. | Anti-cd36 antibodies and their use to treat cancer |
| WO2023080900A1 (en) | 2021-11-05 | 2023-05-11 | Genentech, Inc. | Methods and compositions for classifying and treating kidney cancer |
| TW202342510A (zh) | 2022-02-18 | 2023-11-01 | 英商Rq生物科技有限公司 | 抗體 |
| WO2023192436A1 (en) | 2022-03-31 | 2023-10-05 | Alexion Pharmaceuticals, Inc. | Singleplex or multiplexed assay for complement markers in fresh biological samples |
| EP4514842A1 (en) | 2022-04-29 | 2025-03-05 | AstraZeneca UK Limited | Sars-cov-2 antibodies and methods of using the same |
| WO2023240124A1 (en) | 2022-06-07 | 2023-12-14 | Regeneron Pharmaceuticals, Inc. | Pseudotyped viral particles for targeting tcr-expressing cells |
| EP4554969A1 (en) | 2022-07-15 | 2025-05-21 | Janssen Biotech, Inc. | Material and methods for improved bioengineered pairing of antigen-binding variable regions |
| EP4554619A1 (en) | 2022-07-15 | 2025-05-21 | Danisco US Inc. | Methods for producing monoclonal antibodies |
| CA3266977A1 (en) | 2022-09-06 | 2024-03-14 | Alexion Pharmaceuticals, Inc. | DIAGNOSTIC AND PROGNOSIS BIOMARKER PROFILES IN PATIENTS WITH HEMATOPOIETIC STEM CELL TRANSPLANT-ASSOCIATED THROMBOTIC MICROANGIOPATHY (HSCT-TMA) |
| WO2024077166A1 (en) | 2022-10-05 | 2024-04-11 | Genentech, Inc. | Methods and compositions for classifying and treating lung cancer |
| WO2024077095A1 (en) | 2022-10-05 | 2024-04-11 | Genentech, Inc. | Methods and compositions for classifying and treating bladder cancer |
| WO2024194685A2 (en) | 2023-03-17 | 2024-09-26 | Oxitope Pharma B.V. | Anti-phosphocholine antibodies and methods of use thereof |
| KR20250173600A (ko) | 2023-03-17 | 2025-12-10 | 옥시토프 파마 비.브이. | 항-포스포콜린 항체 및 그 사용 방법 |
| AU2024277678A1 (en) | 2023-05-25 | 2025-11-27 | Dispatch Biotherapeutics, Inc. | Synthetic cancer antigens as targets for treating cancers |
| WO2025072888A2 (en) | 2023-09-28 | 2025-04-03 | Novavax, Inc. | Anti-sars-cov-2 spike (s) antibodies and their use in treating covid-19 |
| WO2025133707A1 (en) | 2023-12-19 | 2025-06-26 | Vectory Therapeutics B.V. | Anti-tdp-43 antibodies and uses thereof |
| WO2025171383A2 (en) | 2024-02-09 | 2025-08-14 | Dispatch Biotherapeutics, Inc. | Engineered cancer antigens and related methods and uses |
| WO2025171388A1 (en) | 2024-02-09 | 2025-08-14 | Dispatch Biotherapeutics, Inc. | Engineered cancer antigens with modified domains and related methods and uses |
| US20250361297A1 (en) | 2024-03-12 | 2025-11-27 | Adaptam Therapeutics, S.L. | Anti- siglec-15 binding molecules and methods of use |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1539188A (en) * | 1987-05-04 | 1988-11-10 | Bristol-Myers Squibb Company | TGF-B2 and novel compositions having anti-neoplastic activity |
| US5571714A (en) * | 1988-12-22 | 1996-11-05 | Celtrix Pharmaceuticals, Inc. | Monoclonal antibodies which bind both transforming growth factors β1 and β2 and methods of use |
| DE69030956T2 (de) * | 1989-09-29 | 1998-01-15 | La Jolla Cancer Research Foundation, La Jolla, Calif. | Inhibierung des Transformierenden Wachstumsfaktors zur Verhinderung der Anhäufung Extrazellulärer Matrix |
| GB9106678D0 (en) * | 1991-03-28 | 1991-05-15 | Ferguson Mark W J | Wound healing |
| DK0590058T3 (da) * | 1991-06-14 | 2004-03-29 | Genentech Inc | Humaniseret heregulin-antistof |
| ES2313867T3 (es) * | 1991-12-02 | 2009-03-16 | Medical Research Council | Produccion de anticuerpos anti-auto de repertorios de segmentos de anticuerpo expresados en la superficie de fagos. |
| AU4111893A (en) * | 1992-04-23 | 1993-11-29 | La Jolla Cancer Research Foundation | Methods for treating vascular disorders by inhibiting the endothelin stimulatory activity of TGFbeta |
| DK0672142T3 (da) * | 1992-12-04 | 2001-06-18 | Medical Res Council | Multivalente og multispecifikke bindingsproteiner samt fremstilling og anvendelse af disse |
| DK0754058T3 (da) * | 1994-03-29 | 2000-02-07 | Univ Manchester | Sårheling |
| GB2288118A (en) * | 1994-03-29 | 1995-10-11 | Univ Manchester | Wound healing composition |
-
1996
- 1996-01-19 GB GBGB9601081.4A patent/GB9601081D0/en active Pending
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- 1996-10-07 JP JP51480997A patent/JP4387458B2/ja not_active Expired - Lifetime
- 1996-10-07 DE DE69611766T patent/DE69611766T2/de not_active Expired - Lifetime
- 1996-10-07 PT PT99102166T patent/PT945464E/pt unknown
- 1996-10-07 EP EP96932730A patent/EP0853661B1/en not_active Expired - Lifetime
- 1996-10-07 AT AT99102166T patent/ATE199091T1/de not_active IP Right Cessation
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- 1996-10-07 WO PCT/GB1996/002450 patent/WO1997013844A1/en not_active Ceased
- 1996-10-07 CA CA002599488A patent/CA2599488A1/en not_active Abandoned
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- 1996-10-07 EP EP99102166A patent/EP0945464B1/en not_active Expired - Lifetime
- 1996-10-07 PT PT96932730T patent/PT853661E/pt unknown
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- 2000-05-18 GR GR20000401123T patent/GR3033436T3/el unknown
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2001
- 2001-04-20 GR GR20010400625T patent/GR3035775T3/el not_active IP Right Cessation
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| EP0853661A1 (en) | 1998-07-22 |
| ES2156035T3 (es) | 2001-06-01 |
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| DK0945464T3 (da) | 2001-05-07 |
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| GR3033436T3 (en) | 2000-09-29 |
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| WO1997013844A1 (en) | 1997-04-17 |
| ATE199091T1 (de) | 2001-02-15 |
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| DK0853661T3 (da) | 2000-08-14 |
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| DE69607191T2 (de) | 2000-09-28 |
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| AU7140596A (en) | 1997-04-30 |
| DE69611766D1 (de) | 2001-03-15 |
| GR3035775T3 (en) | 2001-07-31 |
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| JP4387458B2 (ja) | 2009-12-16 |
| EP0945464B1 (en) | 2001-02-07 |
| CA2233042A1 (en) | 1997-04-17 |
| ES2146020T3 (es) | 2000-07-16 |
| EP0853661B1 (en) | 2000-03-15 |
| GB9601081D0 (en) | 1996-03-20 |
| JP2000500643A (ja) | 2000-01-25 |
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