BRPI0814527B1 - métodos para amplificar uma sequência alvo de ácido nucleico de fita dupla e de fita simples, bem como kit para amplificar uma sequência de ácido nucleico alvo - Google Patents
métodos para amplificar uma sequência alvo de ácido nucleico de fita dupla e de fita simples, bem como kit para amplificar uma sequência de ácido nucleico alvo Download PDFInfo
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- BRPI0814527B1 BRPI0814527B1 BRPI0814527A BRPI0814527A BRPI0814527B1 BR PI0814527 B1 BRPI0814527 B1 BR PI0814527B1 BR PI0814527 A BRPI0814527 A BR PI0814527A BR PI0814527 A BRPI0814527 A BR PI0814527A BR PI0814527 B1 BRPI0814527 B1 BR PI0814527B1
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- nucleic acid
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- single strand
- stranded nucleic
- amplification
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
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- Life Sciences & Earth Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
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| US11/778,018 US9689031B2 (en) | 2007-07-14 | 2007-07-14 | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
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Families Citing this family (130)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7270981B2 (en) | 2002-02-21 | 2007-09-18 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| WO2007096702A2 (en) | 2005-07-25 | 2007-08-30 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
| US8071308B2 (en) | 2006-05-04 | 2011-12-06 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US9689031B2 (en) * | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| WO2010120853A2 (en) * | 2009-04-16 | 2010-10-21 | Padma Arunachalam | Methods and compositions to detect and differentiate small rnas in rna maturation pathway |
| JP5847076B2 (ja) | 2009-05-20 | 2016-01-20 | バイオサイト インコーポレイテッド | Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質 |
| EP2438196B1 (en) | 2009-06-05 | 2016-12-21 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
| EP2443254A2 (en) * | 2009-06-15 | 2012-04-25 | NetBio, Inc. | Improved methods for forensic dna quantitation |
| US20110003703A1 (en) * | 2009-07-01 | 2011-01-06 | Charles Ma | Nucleic Acid Hybridization and Detection Using Enzymatic Reactions on a Microarray |
| EP2287334A1 (de) * | 2009-08-21 | 2011-02-23 | Qiagen GmbH | Verfahren zum Nachweis von Zielnukleinsäuren |
| EP2553124A4 (en) * | 2010-03-30 | 2013-10-02 | Monoquant Pty Ltd | METHOD FOR REGULATING OLIGONUCLEOTIDE FUNCTIONS |
| WO2011137533A1 (en) | 2010-05-05 | 2011-11-10 | The Governing Council Of The University Of Toronto | Method of processing dried samples using digital microfluidic device |
| EP2418286A1 (en) | 2010-08-10 | 2012-02-15 | QIAGEN GmbH | Improved method for isothermal amplification of nucleic acids |
| WO2012021493A2 (en) * | 2010-08-13 | 2012-02-16 | Envirologix Inc. | Compositions and methods for quantifying a nucleic acid sequence in a sample |
| EP2420579A1 (en) * | 2010-08-17 | 2012-02-22 | QIAGEN GmbH | Helicase dependent isothermal amplification using nicking enzymes |
| WO2012038503A1 (en) | 2010-09-23 | 2012-03-29 | Qiagen Gmbh | Method for detecting and/or quantifying human dna |
| SG190377A1 (en) | 2010-11-24 | 2013-06-28 | Kaneka Corp | Amplified nucleic acid detection method and detection device |
| US9845495B2 (en) | 2010-12-10 | 2017-12-19 | Abbott Laboratories | Method and kit for detecting target nucleic acid |
| US9290815B2 (en) | 2011-02-21 | 2016-03-22 | Qiagen Gmbh | Method for quantifying human DNA |
| EP2678443B1 (en) | 2011-02-21 | 2016-11-23 | Qiagen GmbH | Method for quantifying human dna using an internal control |
| SG10201605049QA (en) * | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
| US9352312B2 (en) | 2011-09-23 | 2016-05-31 | Alere Switzerland Gmbh | System and apparatus for reactions |
| US9617590B2 (en) | 2011-09-30 | 2017-04-11 | Stc.Unm | DNA sample preparation and sequencing |
| KR101358416B1 (ko) * | 2012-02-16 | 2014-02-11 | 한국과학기술원 | 리가제 반응과 절단효소 증폭반응을 이용한 표적 유전자 또는 이의 돌연변이 검출방법 |
| RU2601129C2 (ru) | 2012-04-09 | 2016-10-27 | Инвайролоджикс Инк. | Композиции и способы для количественного определения последовательности нуклеиновой кислоты в образце |
| EP2859111B1 (en) * | 2012-06-08 | 2020-07-08 | Ionian Technologies, LLC | Nucleic acid amplifications |
| CN103667252A (zh) * | 2012-09-10 | 2014-03-26 | 思洛生物技术股份有限公司 | 一种核酸扩增方法 |
| CN103173537B (zh) * | 2012-12-21 | 2014-09-10 | 山东出入境检验检疫局检验检疫技术中心 | 大肠埃希氏菌o157:h7的切刻内切酶核酸恒温扩增快速检测试剂盒 |
| CN105143462A (zh) * | 2013-02-05 | 2015-12-09 | 生物纳米基因有限公司 | 用于单分子分析的方法 |
| WO2014164479A1 (en) * | 2013-03-11 | 2014-10-09 | Elitech Holding B.V. | Methods for true isothermal strand displacement amplification |
| US10590474B2 (en) | 2013-03-11 | 2020-03-17 | Elitechgroup B.V. | Methods for true isothermal strand displacement amplification |
| EP3058100A4 (en) * | 2013-10-18 | 2017-04-19 | California Institute of Technology | Enhanced nucleic acid identification and detection |
| WO2015076356A1 (ja) * | 2013-11-22 | 2015-05-28 | 株式会社カネカ | 短鎖ｒｎａの検出方法 |
| JP6739339B2 (ja) | 2014-01-15 | 2020-08-12 | アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ | カバーされた配列変換ｄｎａおよび検出方法 |
| ES2719122T3 (es) | 2014-03-21 | 2019-07-08 | Life Technologies Corp | Ensayo de referencia multi-copia |
| EP3748011B1 (en) | 2014-04-22 | 2024-11-13 | Envirologix Inc. | Compositions and methods for enhancing and/or predicting dna amplification |
| US10100370B2 (en) | 2014-04-30 | 2018-10-16 | Envirologix Inc. | Compositions and methods for detecting huanglongbing |
| ES2759337T3 (es) | 2014-10-14 | 2020-05-08 | Abbott Lab | ADN de conversión de secuencia y ADN amplificador de señal que tiene secuencias espaciadoras de poli-ADN y métodos de detección que los utilizan |
| CN107208137A (zh) * | 2014-10-20 | 2017-09-26 | 龙公司 | 检测rna病毒的组合物和方法 |
| US10604790B2 (en) | 2014-12-24 | 2020-03-31 | Abbott Laboratories | Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same |
| CN105154556B (zh) * | 2015-01-23 | 2018-10-30 | 中国人民解放军第三军医大学第一附属医院 | 实时荧光恒温指数扩增方法 |
| RU2755495C2 (ru) * | 2015-01-30 | 2021-09-16 | Инвайролоджикс Инк. | Молекула субстрата |
| CA2983819A1 (en) | 2015-04-24 | 2016-10-27 | Atila Biosystems Incorporated | Amplification with primers of limited nucleotide composition |
| WO2016197106A1 (en) | 2015-06-05 | 2016-12-08 | Miroculus Inc. | Evaporation management in digital microfluidic devices |
| US10464067B2 (en) | 2015-06-05 | 2019-11-05 | Miroculus Inc. | Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling |
| CN104988235B (zh) * | 2015-07-20 | 2019-01-22 | 北京福德安科技有限公司 | 一种切克酶介导等温扩增小分子信标定量检测技术 |
| CN105154534A (zh) * | 2015-07-30 | 2015-12-16 | 中国人民解放军第三军医大学第一附属医院 | 微小rna的实时恒温指数扩增方法 |
| CN105112507A (zh) * | 2015-07-30 | 2015-12-02 | 中国人民解放军第三军医大学第一附属医院 | 微小rna的数字化恒温检测方法 |
| CN105063190A (zh) * | 2015-07-30 | 2015-11-18 | 中国人民解放军第三军医大学第一附属医院 | 微小rna的固相芯片恒温检测方法 |
| EP3978632A1 (en) | 2015-09-15 | 2022-04-06 | Neogen Corporation | Means for detecting listeria from an environmental sample |
| GB201519565D0 (en) | 2015-11-05 | 2015-12-23 | Alere San Diego Inc | Sample preparation device |
| ES3063374T3 (en) | 2015-10-30 | 2026-04-16 | Abbott Diagnostics Scarborough Inc | Determination of polymorphisms using isothermal nucleic acid amplification |
| US10329601B2 (en) * | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
| WO2017141067A1 (en) | 2016-02-16 | 2017-08-24 | The University Of Tokyo | Method of eliminating background amplification of nucleic acid targets |
| WO2017147486A1 (en) | 2016-02-26 | 2017-08-31 | Alere San Diego Inc. | Redox labeled oligonucleotide probes and their use |
| CA3016582A1 (en) | 2016-03-04 | 2017-09-08 | Alere San Diego, Inc. | Automated nested recombinase polymerase amplification |
| US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US20190176153A1 (en) | 2016-05-18 | 2019-06-13 | Roche Sequencing Solutions, Inc. | Quantitative real time pcr amplification using an electrowetting-based device |
| US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
| KR101820440B1 (ko) * | 2016-06-28 | 2018-01-22 | 한국과학기술원 | 표적핵산의 검출방법 |
| GB201611469D0 (en) * | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| JP2020501107A (ja) | 2016-08-22 | 2020-01-16 | ミロキュラス インコーポレイテッド | デジタルマイクロ流体デバイスにおける並行小滴制御のためのフィードバックシステム |
| US11198910B2 (en) * | 2016-09-02 | 2021-12-14 | New England Biolabs, Inc. | Analysis of chromatin using a nicking enzyme |
| WO2018053174A1 (en) | 2016-09-15 | 2018-03-22 | Abbott Laboratories | Devices and methods for sample analysis |
| US11149304B2 (en) | 2016-09-16 | 2021-10-19 | Qiagen Gmbh | Method for determining nucleic acid degradation in a sample in which at least two overlapping amplicons are produced and two probes are used in the method |
| WO2018054783A1 (en) | 2016-09-16 | 2018-03-29 | Qiagen Gmbh | Method for quantifying and/or detecting human male dna |
| MX2019003674A (es) | 2016-09-30 | 2021-01-08 | Univ California | Enzimas modificadoras de ácido nucleico guiadas por arn y métodos de uso de estas. |
| BR112019006388A2 (pt) | 2016-09-30 | 2019-06-25 | Univ California | enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmas |
| WO2018126082A1 (en) | 2016-12-28 | 2018-07-05 | Miroculis Inc. | Digital microfluidic devices and methods |
| GB201701262D0 (en) | 2017-01-25 | 2017-03-08 | Sense Biodetection Ltd | Nucleic acid detection method |
| BR112019017947A2 (pt) | 2017-02-28 | 2020-05-19 | Alere San Diego Inc | dispositivo microfluídico, leitor configurado para recebê-lo e método |
| US20200017862A1 (en) * | 2017-03-07 | 2020-01-16 | University Of North Carolina Charlotte | Systems and methods for single-strand break signaling and repair in a cell-free system |
| US11623219B2 (en) | 2017-04-04 | 2023-04-11 | Miroculus Inc. | Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets |
| CA3058202A1 (en) * | 2017-04-17 | 2018-10-25 | Montana State University | Switch-like isothermal dna amplification demonstrating a non-linear amplification rate |
| WO2019023133A1 (en) | 2017-07-24 | 2019-01-31 | Miroculus Inc. | DIGITAL MICROFLUIDIC SYSTEMS AND METHODS WITH INTEGRATED PLASMA COLLECTION DEVICE |
| KR20200083444A (ko) * | 2017-08-31 | 2020-07-08 | 아이오니안 테크놀로지스, 엘엘씨 | 호흡기 세포융합 바이러스 종의 니킹 및 연장 증폭 반응(near) |
| JP7341124B2 (ja) | 2017-09-01 | 2023-09-08 | ミロキュラス インコーポレイテッド | デジタルマイクロ流体デバイスおよびその使用方法 |
| WO2019089804A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Casy compositions and methods of use |
| AU2018358051B2 (en) | 2017-11-01 | 2025-01-09 | The Regents Of The University Of California | CasZ compositions and methods of use |
| US11970719B2 (en) | 2017-11-01 | 2024-04-30 | The Regents Of The University Of California | Class 2 CRISPR/Cas compositions and methods of use |
| GB2569965A (en) * | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| ES2937927T3 (es) | 2018-01-29 | 2023-04-03 | St Jude Childrens Res Hospital Inc | Método para la amplificación de ácidos nucleicos |
| GB201804585D0 (en) * | 2018-03-22 | 2018-05-09 | Dnae Diagnostics Ltd | Methods for amplication of nucleic acids with Endonuclease-Mediated shifting equilibrium amplification (EM-SEq) |
| WO2019226919A1 (en) | 2018-05-23 | 2019-11-28 | Miroculus Inc. | Control of evaporation in digital microfluidics |
| AU2019291827A1 (en) * | 2018-06-26 | 2020-12-24 | Massachusetts Institute Of Technology | Crispr double nickase based amplification compositions, systems, and methods |
| CN109055524B (zh) * | 2018-07-24 | 2022-03-15 | 中山大学 | 一种基于DNA组装的恒温条件下同时检测多种microRNA的探针和方法 |
| GB201812149D0 (en) | 2018-07-25 | 2018-09-05 | Sense Biodetection Ltd | Nucleic acid detection method |
| EP3830301B1 (en) | 2018-08-01 | 2024-05-22 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
| CN109136353B (zh) * | 2018-08-21 | 2021-11-16 | 中山大学 | 一种低序列依赖高阶恒温指数扩增检测microRNA的方法 |
| JP7734964B2 (ja) * | 2018-09-26 | 2025-09-08 | ランプロジェン， インコーポレイテッド | タンパク質検出のためのデジタル増幅 |
| CN109321669B (zh) * | 2018-10-29 | 2022-03-15 | 江南大学 | 一种基于嵌合体序列设计和分子信标的荧光检测金黄色葡萄球菌的方法 |
| CN109593863A (zh) * | 2018-11-28 | 2019-04-09 | 江南大学 | 一种链置换型dna聚合酶诱导等温循环扩增反应检测肉类来源的方法 |
| EP3931313A2 (en) | 2019-01-04 | 2022-01-05 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
| CN113543883A (zh) | 2019-01-31 | 2021-10-22 | 米罗库鲁斯公司 | 非结垢组合物以及用于操控和处理包封的微滴的方法 |
| WO2020176816A1 (en) | 2019-02-28 | 2020-09-03 | Miroculus Inc. | Digital microfluidics devices and methods of using them |
| CN119158636A (zh) | 2019-04-08 | 2024-12-20 | 米罗库鲁斯公司 | 多盒式数字微流控装置和使用方法 |
| GB201915346D0 (en) | 2019-06-21 | 2019-12-04 | Lumiradx Tech Ltd | Improvements in or relating to nicking enzymes |
| KR102531590B1 (ko) * | 2019-07-16 | 2023-05-11 | 고려대학교 산학협력단 | 절단효소 인식부위의 미러링 결합에 의한 표적핵산의 검출 방법 및 핵산 검출용 조성물 |
| JP2021019581A (ja) * | 2019-07-25 | 2021-02-18 | 株式会社リコー | 標準核酸試料調製用鋳型核酸分子 |
| WO2021016614A1 (en) | 2019-07-25 | 2021-01-28 | Miroculus Inc. | Digital microfluidics devices and methods of use thereof |
| MX2022001324A (es) | 2019-07-31 | 2022-05-19 | Bioskryb Genomics Inc | Análisis de células individuales. |
| AU2021211931A1 (en) | 2020-01-25 | 2022-07-14 | Sense Biodetection Limited | Virus detection |
| US20230086471A1 (en) * | 2020-02-15 | 2023-03-23 | Agency For Science, Technology And Research | A method to amplify a nucleic acid |
| CN111979303A (zh) * | 2020-08-11 | 2020-11-24 | 上海奕谱生物科技有限公司 | 一种核酸检测试剂盒、方法及其应用 |
| US20240052432A1 (en) | 2020-10-22 | 2024-02-15 | Neogen Corporation | Methods of Detecting Listeria from an Environmental Sample |
| CN112899349B (zh) * | 2020-12-03 | 2022-12-27 | 青岛大学附属医院 | 同时检测一种或多种靶标核酸的可视化核酸检测方法及其应用 |
| CN112646862B (zh) * | 2020-12-28 | 2022-07-15 | 天津市疾病预防控制中心 | 一种新型的自我链置换原理及其多重链置换反应的应用 |
| CN112760362B (zh) * | 2021-02-04 | 2023-07-07 | 中国人民解放军陆军特色医学中心 | 一种用于寡核苷酸扩增的环形信号扩增模板及其应用 |
| CA3218077A1 (en) | 2021-04-29 | 2022-11-03 | Abbott Laboratories | Systems and methods for pooling samples for high-throughput analysis |
| WO2022248874A1 (en) | 2021-05-27 | 2022-12-01 | Lumiradx Uk Ltd | Improvements in or relating to digestion of reaction products |
| WO2022261281A1 (en) * | 2021-06-09 | 2022-12-15 | New England Biolabs, Inc. | Target initiation and amplification of long dna with nuclease and replisome enzymes |
| CN113481283A (zh) * | 2021-06-30 | 2021-10-08 | 上海伯杰医疗科技有限公司北京分公司 | 等温扩增核酸靶序列的方法 |
| WO2023014222A1 (en) | 2021-08-03 | 2023-02-09 | Wageningen Universiteit | Argonaute-based nucleic acid detection system |
| US11857961B2 (en) | 2022-01-12 | 2024-01-02 | Miroculus Inc. | Sequencing by synthesis using mechanical compression |
| EP4565720A1 (en) | 2022-08-04 | 2025-06-11 | Abbott Laboratories | Assays for detecting monkeypox virus |
| WO2024040020A1 (en) | 2022-08-15 | 2024-02-22 | Absci Corporation | Quantitative affinity activity specific cell enrichment |
| WO2025030178A1 (en) | 2023-08-03 | 2025-02-06 | Abbott Laboratories | Isothermal amplification of nucleic acids |
| WO2025049450A1 (en) | 2023-08-28 | 2025-03-06 | Abbott Molecular, Inc. | Assays for detecting bk virus (bkv) |
| WO2025049451A1 (en) | 2023-08-28 | 2025-03-06 | Abbott Molecular, Inc. | Assays for detecting cytomegalovirus (cmv) or epstein-bar virus (ebv) |
| WO2025050131A1 (en) | 2023-09-01 | 2025-03-06 | Abbott Laboratories | Methods of detecting variant nucleic acids |
| WO2025111147A1 (en) | 2023-11-21 | 2025-05-30 | Abbott Laboratories | Two-dimensional matrix droplet array |
| WO2025129195A1 (en) | 2023-12-15 | 2025-06-19 | New England Biolabs, Inc. | Dna polymerases and related methods |
| WO2025137528A1 (en) | 2023-12-20 | 2025-06-26 | Abbott Laboratories | Reducing agent compositions for isothermal amplification of nucleic acids |
| WO2025144700A1 (en) | 2023-12-27 | 2025-07-03 | Absci Corporation | Nanobody library screening using bacterial surface display |
| WO2025171104A1 (en) * | 2024-02-08 | 2025-08-14 | Alere San Diego, Inc. | Universal hairpin probes |
| WO2025240630A1 (en) | 2024-05-14 | 2025-11-20 | Abbott Laboratories | Reagent compositions for isothermal amplification of nucleic acids |
| US20260092310A1 (en) | 2024-09-27 | 2026-04-02 | New England Biolabs, Inc. | Cas12a compositions and methods |
Family Cites Families (116)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6040166A (en) | 1985-03-28 | 2000-03-21 | Roche Molecular Systems, Inc. | Kits for amplifying and detecting nucleic acid sequences, including a probe |
| US5273879A (en) | 1987-07-23 | 1993-12-28 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5455166A (en) * | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| US5556751A (en) | 1991-04-25 | 1996-09-17 | Amoco Corporation | Selective amplification system using Q-β replicase |
| US5846717A (en) | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
| FR2683827B1 (fr) | 1991-11-15 | 1994-03-04 | Institut Nal Sante Recherc Medic | Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique. |
| US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
| US5733733A (en) | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5614389A (en) | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| WO1994003635A1 (en) | 1992-08-04 | 1994-02-17 | Institute Of Molecular Biology & Biotechnology | Rapid amplification and detection of nucleic acids |
| WO1994003624A1 (en) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US5834202A (en) | 1992-08-04 | 1998-11-10 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5457027A (en) | 1993-05-05 | 1995-10-10 | Becton, Dickinson And Company | Internal controls for isothermal nucleic acid amplification reactions |
| US5422252A (en) | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
| US5470723A (en) | 1993-05-05 | 1995-11-28 | Becton, Dickinson And Company | Detection of mycobacteria by multiplex nucleic acid amplification |
| WO1995025180A1 (en) | 1994-03-16 | 1995-09-21 | Gen-Probe Incorporated | Isothermal strand displacement nucleic acid amplification |
| US5547861A (en) | 1994-04-18 | 1996-08-20 | Becton, Dickinson And Company | Detection of nucleic acid amplification |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| US5942391A (en) | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
| ES2185719T3 (es) | 1994-12-23 | 2003-05-01 | Dade Behring Inc | Deteccion de acidos nucleicos por formacion de un producto catalizada por diana. |
| US5556771A (en) | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
| AUPN245295A0 (en) | 1995-04-13 | 1995-05-11 | Johnson & Johnson Research Pty. Limited | Assay for genetic abnormalities |
| AT402203B (de) | 1995-06-13 | 1997-03-25 | Himmler Gottfried Dipl Ing Dr | Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren |
| US5681705A (en) | 1995-08-28 | 1997-10-28 | Schram; James L. | Amplification and detection of mycobacterium avium complex species |
| US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
| US5747255A (en) | 1995-09-29 | 1998-05-05 | Lynx Therapeutics, Inc. | Polynucleotide detection by isothermal amplification using cleavable oligonucleotides |
| US7432048B2 (en) | 1996-11-29 | 2008-10-07 | Third Wave Technologies, Inc. | Reactions on a solid surface |
| US5985557A (en) | 1996-01-24 | 1999-11-16 | Third Wave Technologies, Inc. | Invasive cleavage of nucleic acids |
| CA2249717A1 (en) * | 1996-03-18 | 1997-09-25 | Richard K. Wilkosz | Target nucleic acid sequence amplification |
| US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| JP2000514307A (ja) | 1996-07-16 | 2000-10-31 | ジェン―プローブ・インコーポレーテッド | 増加した標的特異的ｔ▲下ｍ▼を持つ修飾オリゴヌクレオチドを用いた核酸配列の検出および増幅のための方法 |
| US5853990A (en) | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| CA2263815A1 (en) | 1996-08-29 | 1998-03-05 | Koji Kigawa | Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase |
| US6482590B1 (en) | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
| US6197557B1 (en) | 1997-03-05 | 2001-03-06 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
| US6117634A (en) | 1997-03-05 | 2000-09-12 | The Reagents Of The University Of Michigan | Nucleic acid sequencing and mapping |
| EP0975806B1 (en) | 1997-04-03 | 2006-06-21 | Invitrogen Corporation | Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr) |
| WO1998045474A1 (en) | 1997-04-04 | 1998-10-15 | Innogenetics N.V. | Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions |
| US5928869A (en) | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| AR013269A1 (es) | 1997-08-04 | 2000-12-13 | Scras | Producto que contiene por lo menos un rna de doble filamento combinado con por lo menos un agente anti-viral, para la utilizacion terapeutica en eltratamiento de una enfermedad viral, en especial de la hepatitis viral |
| GB9717061D0 (en) | 1997-08-13 | 1997-10-15 | Tepnel Medical Ltd | Amplification of nucleic acids |
| US6077669A (en) | 1997-11-04 | 2000-06-20 | Becton Dickinson And Company | Kit and method for fluorescence based detection assay |
| US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| JP4317953B2 (ja) | 1998-01-22 | 2009-08-19 | 独立行政法人理化学研究所 | Ｄｎａの塩基配列決定方法 |
| US6686150B1 (en) | 1998-01-27 | 2004-02-03 | Clinical Micro Sensors, Inc. | Amplification of nucleic acids with electronic detection |
| GB9827152D0 (en) | 1998-07-03 | 1999-02-03 | Devgen Nv | Characterisation of gene function using double stranded rna inhibition |
| US6287825B1 (en) | 1998-09-18 | 2001-09-11 | Molecular Staging Inc. | Methods for reducing the complexity of DNA sequences |
| WO2000028084A1 (en) | 1998-11-06 | 2000-05-18 | Molecular Biology Resources, Inc. | Isothermal nucleic acid amplification methods |
| CA2361201A1 (en) | 1999-01-28 | 2000-08-03 | Medical College Of Georgia Research Institute, Inc. | Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna |
| DE19956568A1 (de) | 1999-01-30 | 2000-08-17 | Roland Kreutzer | Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens |
| US6316200B1 (en) | 2000-06-08 | 2001-11-13 | Becton, Dickinson And Company | Probes and methods for detection of nucleic acids |
| HK1047109A1 (zh) | 1999-10-15 | 2003-02-07 | University Of Massachusetts | 作为指定基因干预工具的rna干预轨迹基因 |
| US6893819B1 (en) | 2000-11-21 | 2005-05-17 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| GB9927444D0 (en) | 1999-11-19 | 2000-01-19 | Cancer Res Campaign Tech | Inhibiting gene expression |
| US6783934B1 (en) | 2000-05-01 | 2004-08-31 | Cepheid, Inc. | Methods for quantitative analysis of nucleic acid amplification reaction |
| US6191267B1 (en) * | 2000-06-02 | 2001-02-20 | New England Biolabs, Inc. | Cloning and producing the N.BstNBI nicking endonuclease |
| DE10028837A1 (de) | 2000-06-15 | 2001-12-20 | Roche Diagnostics Gmbh | Verfahren zum Nachweis von Influenza A/B-Viren |
| US6350580B1 (en) | 2000-10-11 | 2002-02-26 | Stratagene | Methods for detection of a target nucleic acid using a probe comprising secondary structure |
| WO2002034949A2 (en) | 2000-10-27 | 2002-05-02 | Molecular Staging Inc. | Methods for identifying genes associated with diseases or specific phenotypes |
| WO2002061133A2 (en) | 2000-11-09 | 2002-08-08 | Yale University | Nucleic acid detection using structured probes |
| US7309573B2 (en) | 2000-11-21 | 2007-12-18 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
| US6660475B2 (en) | 2000-12-15 | 2003-12-09 | New England Biolabs, Inc. | Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof |
| US6958217B2 (en) | 2001-01-24 | 2005-10-25 | Genomic Expression Aps | Single-stranded polynucleotide tags |
| CA2444649C (en) | 2001-04-20 | 2012-10-02 | The Penn State Research Foundation | Methods for nucleic acid manipulation |
| US20030138800A1 (en) * | 2001-07-15 | 2003-07-24 | Keck Graduate Institute | Exponential amplification of nucleic acids using nicking agents |
| WO2003008624A2 (en) | 2001-07-15 | 2003-01-30 | Keck Graduate Institute | Nucleic acid amplification using nicking agents |
| WO2003008642A2 (en) | 2001-07-15 | 2003-01-30 | Keck Graduate Institute | Amplification of nucleic acid fragments using nicking agents |
| US6632611B2 (en) | 2001-07-20 | 2003-10-14 | Affymetrix, Inc. | Method of target enrichment and amplification |
| EP1419275A4 (en) | 2001-08-02 | 2007-05-30 | Wayne M Barnes | HOT START METHOD USING MAGNESIUM PRECIPITATE FOR MOLECULAR MANIPULATION OF NUCLEIC ACIDS |
| US6403341B1 (en) | 2001-08-02 | 2002-06-11 | Wayne M. Barnes | Magnesium precipitate hot start method for PCR |
| US20040038256A1 (en) | 2001-10-01 | 2004-02-26 | Keck Graduate Institute | Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization |
| US20040058349A1 (en) | 2001-10-01 | 2004-03-25 | Jeffrey Van Ness | Methods for identifying nucleotides at defined positions in target nucleic acids |
| US6617137B2 (en) | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
| US7297485B2 (en) | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
| US6977148B2 (en) | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US7270981B2 (en) | 2002-02-21 | 2007-09-18 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| GB0207063D0 (en) | 2002-03-26 | 2002-05-08 | Amersham Biosciences Uk Ltd | Immobilised probes |
| CA2498764C (en) | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
| US7662594B2 (en) | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
| US20060160759A1 (en) | 2002-09-28 | 2006-07-20 | Jianzhu Chen | Influenza therapeutic |
| WO2004067726A2 (en) | 2003-01-29 | 2004-08-12 | Keck Graduate Institute | Isothermal reactions for the amplification of oligonucleotides |
| WO2004067764A2 (en) | 2003-01-29 | 2004-08-12 | Keck Graduate Institute | Nucleic acid sequencing using nicking agents |
| US20040209299A1 (en) | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
| CN101410530B (zh) | 2003-04-18 | 2013-03-27 | 贝克顿·迪金森公司 | 免疫-扩增 |
| EP1618214A4 (en) | 2003-04-25 | 2008-02-13 | Becton Dickinson Co | DETECTION OF TYPES 1 AND 2 OF HERPES SIMPLEX VIRUS BY AMPLIFICATION OF NUCLEIC ACID |
| US20050266417A1 (en) | 2003-09-12 | 2005-12-01 | Francis Barany | Methods for identifying target nucleic acid molecules |
| US20050069939A1 (en) | 2003-09-26 | 2005-03-31 | Youxiang Wang | Amplification of polynucleotides by rolling circle amplification |
| US7314714B2 (en) | 2003-12-19 | 2008-01-01 | Affymetrix, Inc. | Method of oligonucleotide synthesis |
| EP2380993B1 (en) | 2004-03-08 | 2015-12-23 | Rubicon Genomics, Inc. | Method for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation |
| US20050233332A1 (en) | 2004-04-14 | 2005-10-20 | Collis Matthew P | Multiple fluorophore detector system |
| EP3985125A1 (en) | 2004-06-01 | 2022-04-20 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification |
| GB2416352B (en) | 2004-07-21 | 2009-01-28 | Bioline Ltd | A method for performing the hot start of enzymatic reactions |
| AU2005286876A1 (en) * | 2004-09-20 | 2006-03-30 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Multiple mode multiplex reaction quenching method |
| EP2471805A3 (en) | 2005-05-06 | 2013-01-16 | Gen-Probe Incorporated | Compositions and assays to specifically detect nucleic acid of influenza virus A or B |
| US20070020639A1 (en) | 2005-07-20 | 2007-01-25 | Affymetrix, Inc. | Isothermal locus specific amplification |
| WO2007096702A2 (en) | 2005-07-25 | 2007-08-30 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
| WO2007018601A1 (en) | 2005-08-02 | 2007-02-15 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| JP5106416B2 (ja) | 2006-01-06 | 2012-12-26 | アジレント・テクノロジーズ・インク | 濃縮（ｐａｃｋｅｄ）ＤＮＡポリメラーゼを含む核酸複製のための反応緩衝液組成物 |
| CA3031887C (en) | 2006-02-13 | 2023-02-07 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Primers and probes for detection and discrimination of types and subtypes of influenza viruses |
| CN101421417B (zh) | 2006-02-27 | 2013-04-03 | 霍夫曼-拉罗奇有限公司 | 经由镁螯合的pcr热启动 |
| CN100489112C (zh) | 2006-03-10 | 2009-05-20 | 杭州优思达生物技术有限公司 | 切口酶扩增靶核酸序列的方法及用于扩增靶核酸序列的试剂盒及其应用 |
| US20090092967A1 (en) * | 2006-06-26 | 2009-04-09 | Epoch Biosciences, Inc. | Method for generating target nucleic acid sequences |
| US20080096257A1 (en) | 2006-08-15 | 2008-04-24 | Zuxu Yao | Methods for Rapid, Single-Step Strand Displacement Amplification of Nucleic Acids |
| WO2009024834A2 (en) | 2006-12-05 | 2009-02-26 | Rosetta Genomics Ltd | Nucleic acids involved in viral infection |
| US8202972B2 (en) * | 2007-01-10 | 2012-06-19 | General Electric Company | Isothermal DNA amplification |
| US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| CA2786473A1 (en) | 2010-01-08 | 2011-07-14 | Qiagen Gaithersburg, Inc. | Materials and methods for isothermal nucleic acid amplification |
| KR101870311B1 (ko) | 2012-03-09 | 2018-06-25 | (주)바이오니아 | 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물 |
| EP3099810B1 (en) | 2014-01-31 | 2019-05-01 | Qiagen GmbH | Cation chelator hot start |
| WO2016004333A2 (en) | 2014-07-02 | 2016-01-07 | Promega Corporation | Reversible metal ion chelators |
| US10329601B2 (en) * | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
| KR20200083444A (ko) * | 2017-08-31 | 2020-07-08 | 아이오니안 테크놀로지스, 엘엘씨 | 호흡기 세포융합 바이러스 종의 니킹 및 연장 증폭 반응(near) |
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