ES2185719T3 - Deteccion de acidos nucleicos por formacion de un producto catalizada por diana. - Google Patents
Deteccion de acidos nucleicos por formacion de un producto catalizada por diana.Info
- Publication number
- ES2185719T3 ES2185719T3 ES95944648T ES95944648T ES2185719T3 ES 2185719 T3 ES2185719 T3 ES 2185719T3 ES 95944648 T ES95944648 T ES 95944648T ES 95944648 T ES95944648 T ES 95944648T ES 2185719 T3 ES2185719 T3 ES 2185719T3
- Authority
- ES
- Spain
- Prior art keywords
- analyte
- polynucleotidic
- fragment
- detection
- oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
Abstract
SE DESCRIBE UN PROCEDIMIENTO PARA MODIFICAR UN OLIGONUCLEOTIDO, TENIENDO DICHO PROCEDIMIENTO UNA APLICACION EN LA DETECCION DE UN ANALITO POLINUCLEOTIDICO. UN OLIGONUCLEOTIDO SE HIBRIDA DE FORMA REVERSIBLE CON UN POLINUCLEOTIDO, POR EJEMPLO, UN ANALITO POLINUCLEOTIDICO, EN PRESENCIA DE UNA 5 CONDICIONES ISOTERMAS. EL ANALITO POLINUCLEOTIDICO ACTUA COMO ELEMENTO DE RECONOCIMIENTO PARA PERMITIR QUE UNA 5 ESCINDA EL OLIGONUCLEOTIDO PARA SUMINISTRAR (I) UN PRIMER FRAGMENTO QUE ES SUSTANCIALMENTE NO HIBRIDABLE CON EL ANALITO POLINUCLEOTIDICO, Y (II) UN SEGUNDO FRAGMENTO QUE SE ENCUENTRA 3 CON RESPECTO AL PRIMER FRAGMENTO (EN EL OLIGONUCLEOTIDO INTACTO) Y QUE ES SUSTANCIALMENTE HIBRIDABLE CON EL ANALITO POLINUCLEOTIDICO. SE OBTIENE AL MENOS UNA EXCESO MOLAR EN 100 VECES DEL PRIMER FRAGMENTO Y/O EL SEGUNDO FRAGMENTO EN RELACION A LA CANTIDAD MOLAR DEL ANALITO POLINUCLEOTIDICO. SE DETECTA LA PRESENCIA DEL PRIMER FRAGMENTO Y/O DEL SEGUNDO FRAGMENTO, INDICANDO SU PRESENCIA LA PRESENCIA DEL ANALITO POLINUCLEOTIDICO. EL PROCEDIMIENTO ENCUENTRA UNA APLICACION PARTICULAR EN LA DETECCION DE UN ANALITO POLINUCLEOTIDICO, TAL COMO EL ADN. TAMBIEN SE DESCRIBEN ESTUCHES DE ENSAYO PARA LLEVAR A CABO LOS PROCEDIMIENTOS SEGUN LA PRESENTE INVENCION.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36316994A | 1994-12-23 | 1994-12-23 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2185719T3 true ES2185719T3 (es) | 2003-05-01 |
Family
ID=23429108
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES95944648T Expired - Lifetime ES2185719T3 (es) | 1994-12-23 | 1995-12-22 | Deteccion de acidos nucleicos por formacion de un producto catalizada por diana. |
Country Status (5)
Country | Link |
---|---|
US (7) | US5792614A (es) |
EP (2) | EP0842294B1 (es) |
DE (1) | DE69528670T2 (es) |
ES (1) | ES2185719T3 (es) |
WO (1) | WO1996020287A2 (es) |
Families Citing this family (103)
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US20030219755A1 (en) * | 2002-05-24 | 2003-11-27 | Nanibhushan Dattagupta | Compositions and methods for performing hybridization assays using target enhanced signal amplification (TESA) |
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-
1995
- 1995-12-22 EP EP95944648A patent/EP0842294B1/en not_active Expired - Lifetime
- 1995-12-22 WO PCT/US1995/016771 patent/WO1996020287A2/en active IP Right Grant
- 1995-12-22 DE DE69528670T patent/DE69528670T2/de not_active Expired - Lifetime
- 1995-12-22 EP EP02008466A patent/EP1260593A3/en not_active Withdrawn
- 1995-12-22 ES ES95944648T patent/ES2185719T3/es not_active Expired - Lifetime
-
1996
- 1996-08-02 US US08/691,627 patent/US5792614A/en not_active Expired - Lifetime
-
1998
- 1998-01-30 US US09/015,949 patent/US6110677A/en not_active Expired - Lifetime
-
1999
- 1999-11-15 US US09/440,363 patent/US6121001A/en not_active Expired - Lifetime
-
2000
- 2000-06-30 US US09/608,721 patent/US6368803B1/en not_active Expired - Fee Related
-
2001
- 2001-11-20 US US09/989,757 patent/US20020155548A1/en not_active Abandoned
-
2005
- 2005-02-09 US US11/054,255 patent/US20050191676A1/en not_active Abandoned
-
2007
- 2007-10-09 US US11/869,054 patent/US20080213767A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US6368803B1 (en) | 2002-04-09 |
DE69528670D1 (de) | 2002-11-28 |
EP0842294A2 (en) | 1998-05-20 |
WO1996020287A3 (en) | 1996-09-06 |
US6110677A (en) | 2000-08-29 |
DE69528670T2 (de) | 2004-03-11 |
US5792614A (en) | 1998-08-11 |
US20080213767A1 (en) | 2008-09-04 |
US6121001A (en) | 2000-09-19 |
US20050191676A1 (en) | 2005-09-01 |
EP0842294B1 (en) | 2002-10-23 |
EP1260593A2 (en) | 2002-11-27 |
EP1260593A3 (en) | 2003-12-03 |
WO1996020287A2 (en) | 1996-07-04 |
US20020155548A1 (en) | 2002-10-24 |
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