ES2175097T3 - Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde. - Google Patents

Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde.

Info

Publication number
ES2175097T3
ES2175097T3 ES96917989T ES96917989T ES2175097T3 ES 2175097 T3 ES2175097 T3 ES 2175097T3 ES 96917989 T ES96917989 T ES 96917989T ES 96917989 T ES96917989 T ES 96917989T ES 2175097 T3 ES2175097 T3 ES 2175097T3
Authority
ES
Spain
Prior art keywords
detection
mold
formation
nucleic acids
diana
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96917989T
Other languages
English (en)
Inventor
Edwin F Ullman
Linda Marie Western
Samuel J Rose
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Dade Behring Marburg GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Behring Marburg GmbH filed Critical Dade Behring Marburg GmbH
Application granted granted Critical
Publication of ES2175097T3 publication Critical patent/ES2175097T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE REVELA UN METODO PARA LA DETECCION DE UNA SECUENCIA POLINUCLEOTIDICA DIANA. EL METODO COMPRENDE LA INCUBACION DE UN OLIGONUCLEOTIDO CON LA SECUENCIA POLINUCLEOTIDICA DIANA Y UNA POLIMERASA DE NUCLEOTIDOS BAJO CONDICIONES ISOTERMICAS DONDE AL MENOS UN NUCLEOTIDO SE AÑADE AL EXTREMO 3 -TERMINAL DEL OLIGONUCLEOTIDO PARA PROPORCIONAR UN OLIGONUCLEOTIDO EXTENDIDO QUE TIENE LOS NUCLEOTIDOS ADICIONALES. LA PRESENCIA DE OLIGONUCLEOTIDOS EXTENDIDOS SE DETECTA COMO UNA INDICACION DE LA PRESENCIA DE LA SECUENCIA POLINUCLEOTIDA DIANA. EL METODO TIENE PARTICULAR APLICACION PARA LA DETECCION DE ADN.
ES96917989T 1995-06-07 1996-06-05 Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde. Expired - Lifetime ES2175097T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/486,301 US5882867A (en) 1995-06-07 1995-06-07 Detection of nucleic acids by formation of template-dependent product

Publications (1)

Publication Number Publication Date
ES2175097T3 true ES2175097T3 (es) 2002-11-16

Family

ID=23931346

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96917989T Expired - Lifetime ES2175097T3 (es) 1995-06-07 1996-06-05 Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde.

Country Status (7)

Country Link
US (1) US5882867A (es)
EP (1) EP0857218B1 (es)
JP (1) JPH11510369A (es)
CA (1) CA2223774A1 (es)
DE (1) DE69620903T2 (es)
ES (1) ES2175097T3 (es)
WO (1) WO1996040999A1 (es)

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Also Published As

Publication number Publication date
WO1996040999A1 (en) 1996-12-19
CA2223774A1 (en) 1996-12-19
EP0857218A1 (en) 1998-08-12
JPH11510369A (ja) 1999-09-14
US5882867A (en) 1999-03-16
EP0857218B1 (en) 2002-04-24
DE69620903T2 (de) 2002-11-21
DE69620903D1 (de) 2002-05-29

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