ES2175097T3 - Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde. - Google Patents

Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde.

Info

Publication number
ES2175097T3
ES2175097T3 ES96917989T ES96917989T ES2175097T3 ES 2175097 T3 ES2175097 T3 ES 2175097T3 ES 96917989 T ES96917989 T ES 96917989T ES 96917989 T ES96917989 T ES 96917989T ES 2175097 T3 ES2175097 T3 ES 2175097T3
Authority
ES
Spain
Prior art keywords
detection
mold
formation
nucleic acids
diana
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96917989T
Other languages
English (en)
Inventor
Edwin F Ullman
Linda Marie Western
Samuel J Rose
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Dade Behring Marburg GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Behring Marburg GmbH filed Critical Dade Behring Marburg GmbH
Application granted granted Critical
Publication of ES2175097T3 publication Critical patent/ES2175097T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE REVELA UN METODO PARA LA DETECCION DE UNA SECUENCIA POLINUCLEOTIDICA DIANA. EL METODO COMPRENDE LA INCUBACION DE UN OLIGONUCLEOTIDO CON LA SECUENCIA POLINUCLEOTIDICA DIANA Y UNA POLIMERASA DE NUCLEOTIDOS BAJO CONDICIONES ISOTERMICAS DONDE AL MENOS UN NUCLEOTIDO SE AÑADE AL EXTREMO 3 -TERMINAL DEL OLIGONUCLEOTIDO PARA PROPORCIONAR UN OLIGONUCLEOTIDO EXTENDIDO QUE TIENE LOS NUCLEOTIDOS ADICIONALES. LA PRESENCIA DE OLIGONUCLEOTIDOS EXTENDIDOS SE DETECTA COMO UNA INDICACION DE LA PRESENCIA DE LA SECUENCIA POLINUCLEOTIDA DIANA. EL METODO TIENE PARTICULAR APLICACION PARA LA DETECCION DE ADN.
ES96917989T 1995-06-07 1996-06-05 Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde. Expired - Lifetime ES2175097T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/486,301 US5882867A (en) 1995-06-07 1995-06-07 Detection of nucleic acids by formation of template-dependent product

Publications (1)

Publication Number Publication Date
ES2175097T3 true ES2175097T3 (es) 2002-11-16

Family

ID=23931346

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96917989T Expired - Lifetime ES2175097T3 (es) 1995-06-07 1996-06-05 Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde.

Country Status (7)

Country Link
US (1) US5882867A (es)
EP (1) EP0857218B1 (es)
JP (1) JPH11510369A (es)
CA (1) CA2223774A1 (es)
DE (1) DE69620903T2 (es)
ES (1) ES2175097T3 (es)
WO (1) WO1996040999A1 (es)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6872816B1 (en) * 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6706471B1 (en) * 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US6913881B1 (en) 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
US6090606A (en) * 1996-01-24 2000-07-18 Third Wave Technologies, Inc. Cleavage agents
US7432048B2 (en) * 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US7527928B2 (en) * 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
US7256020B2 (en) * 1996-11-29 2007-08-14 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US7122364B1 (en) 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US7195871B2 (en) * 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US6780982B2 (en) * 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
JP4362150B2 (ja) 1996-11-29 2009-11-11 サード ウェーブ テクノロジーズ，インコーポレーテッド Ｆｅｎ−１エンドヌクレアーゼ、混合物、および開裂方法
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
KR100527265B1 (ko) 1999-09-13 2005-11-09 뉴젠 테크놀로지스 인코포레이티드 폴리뉴클레오티드 서열의 선형 등온 증폭을 위한 방법 및조성물
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US6355438B1 (en) * 1999-11-12 2002-03-12 Isis Pharmaceuticals, Inc. Method for quantitating oligonucleotides
EP1356094B1 (en) * 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
CA2428798C (en) * 2000-11-15 2013-09-03 Third Wave Technologies, Inc. Fen-1 endonucleases from archaeoglobus veneficus
ATE475720T1 (de) * 2000-12-13 2010-08-15 Nugen Technologies Inc Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben
WO2002072773A2 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
DE60220025T2 (de) 2001-03-09 2008-01-17 Nugen Technologies, Inc., San Carlos Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
US6696254B2 (en) * 2001-11-21 2004-02-24 Kimberly-Clark Worldwide, Inc. Detection and identification of enteric bacteria
CA2471198A1 (en) * 2001-12-20 2003-07-03 Merck & Co., Inc. Identification of novel polymorphic sites in the human mglur8 gene and uses thereof
EP1546392A4 (en) * 2002-08-02 2006-05-24 Stratatech Corp DETECTION OF SPECIFIC DNA FROM SPECIES
AU2003297541A1 (en) 2002-12-18 2004-07-14 Third Wave Technologies, Inc. Detection of small nucleic acids
US7851150B2 (en) * 2002-12-18 2010-12-14 Third Wave Technologies, Inc. Detection of small nucleic acids
US8206904B2 (en) * 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
US7297780B2 (en) 2003-01-06 2007-11-20 Third Wave Technologies, Inc. Reactive functional groups for preparation of modified nucleic acid
KR100890885B1 (ko) 2003-03-31 2009-03-31 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
WO2004092418A2 (en) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US7462451B2 (en) * 2004-04-26 2008-12-09 Third Wave Technologies, Inc. Compositions for modifying nucleic acids
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US7939258B2 (en) * 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US20070207455A1 (en) * 2005-11-17 2007-09-06 Third Wave Technologies, Inc. Compositions and methods for detecting an HCV-1 subtype
KR100652903B1 (ko) * 2005-12-21 2006-12-04 한국과학기술연구원 초흡수성 고분자를 함유한 제습제의 제조 방법 및 그 제조장치
US7674924B2 (en) * 2006-05-22 2010-03-09 Third Wave Technologies, Inc. Compositions, probes, and conjugates and uses thereof
WO2007143097A1 (en) 2006-06-01 2007-12-13 Third Wave Technologies, Inc. Detection of nucleic acids
GB0713183D0 (en) * 2007-07-06 2007-08-15 King S College London Method
AU2008296038B2 (en) * 2007-09-07 2012-08-09 Third Wave Technologies, Inc. Methods and applications for target quantification
EP2245461A1 (en) * 2008-01-29 2010-11-03 Victor Manneh High specifity immuno-amplification
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
JP5651022B2 (ja) * 2008-03-15 2015-01-07 ホロジック，インコーポレーテッド 増幅反応中の核酸分子を分析するための組成物及び方法
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
US8208909B2 (en) 2008-06-02 2012-06-26 West Corporation System, apparatus and method for availing a mobile call of address information
US20110091931A1 (en) * 2009-04-24 2011-04-21 Colby Pharmaceutical Company Methods and Kits for Determining Oxygen Free Radical (OFR) Levels in Animal and Human Tissues as a Prognostic Marker for Cancer and Other Pathophysiologies
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US8394589B2 (en) 2009-12-21 2013-03-12 Northwestern University Methods for diagnosing scapuloperoneal spinal muscular atrophy or Charcot-Marie-Tooth disease type 2C by detecting mutations in TRPV4
US10233501B2 (en) 2010-10-19 2019-03-19 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US20150225792A1 (en) 2014-01-17 2015-08-13 Northwestern University Compositions and methods for identifying depressive disorders
US20150218639A1 (en) 2014-01-17 2015-08-06 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US10093981B2 (en) 2010-10-19 2018-10-09 Northwestern University Compositions and methods for identifying depressive disorders
CN110129436A (zh) 2011-02-02 2019-08-16 精密科学公司 Dna甲基化的数字序列分析
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
EP2707510B1 (en) 2011-05-12 2017-07-12 Exact Sciences Corporation Isolation of nucleic acids
US8993341B2 (en) 2011-05-12 2015-03-31 Exact Sciences Corporation Removal of PCR inhibitors
US8808990B2 (en) 2011-05-12 2014-08-19 Exact Sciences Corporation Serial isolation of multiple DNA targets from stool
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
EP2794904B1 (en) 2011-12-22 2017-09-06 Ibis Biosciences, Inc. Amplification of a sequence from a ribonucleic acid
GB2533882B (en) 2012-01-26 2016-10-12 Nugen Tech Inc Method of enriching and sequencing nucleic acids of interest using massively parallel sequencing
AU2013205064B2 (en) 2012-06-04 2015-07-30 Gen-Probe Incorporated Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
US9957549B2 (en) 2012-06-18 2018-05-01 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9212392B2 (en) 2012-09-25 2015-12-15 Exact Sciences Corporation Normalization of polymerase activity
EP4234721A3 (en) 2013-03-14 2023-10-18 Mayo Foundation for Medical Education and Research Detecting neoplasm
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
WO2015042446A2 (en) 2013-09-20 2015-03-26 The Regents Of The University Of Michigan Compositions and methods for the analysis of radiosensitivity
US10253358B2 (en) 2013-11-04 2019-04-09 Exact Sciences Development Company, Llc Multiple-control calibrators for DNA quantitation
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US9909181B2 (en) 2013-12-13 2018-03-06 Northwestern University Biomarkers for post-traumatic stress states
EP3084004A4 (en) 2013-12-19 2017-08-16 Exact Sciences Corporation Synthetic nucleic acid control molecules
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
ES2879964T3 (es) 2014-12-12 2021-11-23 Exact Sciences Dev Co Llc Composiciones y métodos para realizar ensayos de detección de metilación
US10011878B2 (en) 2014-12-12 2018-07-03 Exact Sciences Development Company Compositions and methods for performing methylation detection assays
US10435755B2 (en) 2015-03-27 2019-10-08 Exact Sciences Development Company, Llc Detecting esophageal disorders
EP3368686A1 (en) 2015-10-30 2018-09-05 Exact Sciences Development Company, LLC Multiplex amplification detection assay and isolation and detection of dna from plasma
US20170321286A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by amplification of rna sequences
WO2017192221A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by analysis of methylated dna
WO2018017710A1 (en) 2016-07-19 2018-01-25 Exact Sciences Development Company, Llc Nucleic acid control molecules from non-human organisms
EP3978624A1 (en) 2016-07-19 2022-04-06 Exact Sciences Corporation Methylated control dna
ES2956257T3 (es) 2016-09-02 2023-12-18 Mayo Found Medical Education & Res Detección de carcinoma hepatocelular
AU2018211956A1 (en) 2017-01-27 2019-07-25 Exact Sciences Corporation Detection of colon neoplasia by analysis of methylated DNA
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
JP7348603B2 (ja) 2018-04-02 2023-09-21 エニュメラ・モレキュラー・インコーポレイテッド 核酸分子を計数するための方法、システム、および組成物
US11408030B2 (en) 2018-09-10 2022-08-09 Andy Madrid Test for detecting Alzheimer's disease
EP3947718A4 (en) 2019-04-02 2022-12-21 Enumera Molecular, Inc. METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES
CA3154354A1 (en) 2019-10-31 2021-05-06 William R. Taylor Detecting ovarian cancer
US20230357852A1 (en) 2020-08-19 2023-11-09 Mayo Foundation For Medical Education And Research Detecting non-hodgkin lymphoma
JP2023543424A (ja) 2020-09-21 2023-10-16 ビオラ・セラピューティクス・インコーポレイテッド 無細胞ｄｎａを単離するための組成物および方法
EP4314286A1 (en) 2021-08-06 2024-02-07 Sansure Biotech Inc. Compositions for liquefying a viscous biological sample, combination products, liquefying agents, and kits thereof, and methods and application thereof

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5008182A (en) * 1986-01-10 1991-04-16 Cetus Corporation Detection of AIDS associated virus by polymerase chain reaction
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
EP0295398B1 (de) * 1987-06-15 1991-09-18 Firma Andreas Stihl Startautomatik für einen Verbrennungsmotor, insbesondere für den von Hand zu startenden Motor eines Arbeitsgerätes
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
GB9009903D0 (en) * 1990-05-02 1990-06-27 Ici Plc Assay for specific nucleic acid sequences
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
EP0517361A1 (en) * 1991-05-30 1992-12-09 Amoco Corporation A method for detecting and identifying pathogenic organisms using target sequences as detectors
WO1993022461A1 (en) * 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
AU4770393A (en) * 1992-07-02 1994-01-31 Diagenetics Gamidor Ltd Methods of single nucleotide primer extension to detect specific alleles and kits therefor
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
DE69409646T2 (de) * 1993-06-09 1998-08-06 Gamera Bioscience Corp Magnetische zyklusreaktionen
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification

Also Published As

Publication number Publication date
EP0857218B1 (en) 2002-04-24
EP0857218A1 (en) 1998-08-12
DE69620903T2 (de) 2002-11-21
CA2223774A1 (en) 1996-12-19
JPH11510369A (ja) 1999-09-14
WO1996040999A1 (en) 1996-12-19
DE69620903D1 (de) 2002-05-29
US5882867A (en) 1999-03-16

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