BRPI0517381A - componentes ortogonais de tradução para a incorporação in vivo de aminoácidos não naturais - Google Patents

componentes ortogonais de tradução para a incorporação in vivo de aminoácidos não naturais

Info

Publication number
BRPI0517381A
BRPI0517381A BRPI0517381-7A BRPI0517381A BRPI0517381A BR PI0517381 A BRPI0517381 A BR PI0517381A BR PI0517381 A BRPI0517381 A BR PI0517381A BR PI0517381 A BRPI0517381 A BR PI0517381A
Authority
BR
Brazil
Prior art keywords
amino acids
unnatural amino
orthogonal
vivo incorporation
translation components
Prior art date
Application number
BRPI0517381-7A
Other languages
English (en)
Inventor
Peter Schultz
Lital Alfonta
Jonathan R Chittuluru
Alexander Deiters
Dan Groff
Daniel Summerer
Meng-Lin Tsao
Jiangyun Wang
Ning Wu
Jianming Xie
Huaqiang Zeng
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of BRPI0517381A publication Critical patent/BRPI0517381A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Ecology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

COMPONENTES ORTOGONAIS DE TRADUçãO PARA A INCORPORAçãO IN VIVO DE AMINOáCIDOS NãO NATURAIS. A presente invenção se refere a pares ortogonais de RNAts e aminoacil-RNAt sintetases que podem incorporar aminoácidos não naturais nas proteínas produzidas em células hospedeiras eubacterianas, tais como E. coli, em um hospedeiro eucariótico tal como uma célula de levedura. A invenção propõe, por exemplo, sem limitação, sintetases ortogonais inéditas, métodos para a identificação e preparação de sintetases inéditas, métodos para a produção de proteínas que contêm aminoácidos não naturais e sistemas de tradução.
BRPI0517381-7A 2004-10-27 2005-10-27 componentes ortogonais de tradução para a incorporação in vivo de aminoácidos não naturais BRPI0517381A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62273804P 2004-10-27 2004-10-27
PCT/US2005/039210 WO2006110182A2 (en) 2004-10-27 2005-10-27 Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Publications (1)

Publication Number Publication Date
BRPI0517381A true BRPI0517381A (pt) 2008-10-07

Family

ID=37087466

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0517381-7A BRPI0517381A (pt) 2004-10-27 2005-10-27 componentes ortogonais de tradução para a incorporação in vivo de aminoácidos não naturais

Country Status (10)

Country Link
US (2) US8216804B2 (pt)
EP (3) EP2380982B1 (pt)
JP (2) JP5135504B2 (pt)
CN (2) CN101048506B (pt)
AU (1) AU2005330514B2 (pt)
BR (1) BRPI0517381A (pt)
CA (1) CA2583735A1 (pt)
IL (2) IL182495A0 (pt)
MX (1) MX2007004892A (pt)
WO (1) WO2006110182A2 (pt)

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Also Published As

Publication number Publication date
AU2005330514B2 (en) 2011-05-12
CN101048506A (zh) 2007-10-03
EP1807515A2 (en) 2007-07-18
WO2006110182A2 (en) 2006-10-19
US20130244245A1 (en) 2013-09-19
EP1807515A4 (en) 2008-06-18
EP2383340A2 (en) 2011-11-02
CN102827827A (zh) 2012-12-19
EP2383340B1 (en) 2014-07-16
JP5135504B2 (ja) 2013-02-06
IL211229A0 (en) 2011-04-28
EP2380982A1 (en) 2011-10-26
CA2583735A1 (en) 2006-10-19
JP2008517631A (ja) 2008-05-29
CN101048506B (zh) 2013-06-26
WO2006110182A3 (en) 2006-12-21
US20080233611A1 (en) 2008-09-25
AU2005330514A1 (en) 2006-10-19
US9102932B2 (en) 2015-08-11
IL182495A0 (en) 2007-09-20
JP2012254073A (ja) 2012-12-27
EP2380982B1 (en) 2014-07-16
EP2383340A3 (en) 2012-03-21
JP5539948B2 (ja) 2014-07-02
CN102827827B (zh) 2014-10-29
US8216804B2 (en) 2012-07-10
MX2007004892A (es) 2007-08-16

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