BR112017024649A2 - métodos para a transformação in planta de plantas e processos de fabricação e produtos com base nelas e obteníveis a partir delas - Google Patents

métodos para a transformação in planta de plantas e processos de fabricação e produtos com base nelas e obteníveis a partir delas

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Publication number
BR112017024649A2
BR112017024649A2 BR112017024649A BR112017024649A BR112017024649A2 BR 112017024649 A2 BR112017024649 A2 BR 112017024649A2 BR 112017024649 A BR112017024649 A BR 112017024649A BR 112017024649 A BR112017024649 A BR 112017024649A BR 112017024649 A2 BR112017024649 A2 BR 112017024649A2
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BR
Brazil
Prior art keywords
methods
plant
plants
transformation
plant transformation
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Application number
BR112017024649A
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English (en)
Inventor
Harling Hinrich
Niessen Markus
Martin-Ortigosa Susana
Original Assignee
Kws Saat Se & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from DE102015006335.9A external-priority patent/DE102015006335A1/de
Priority claimed from DE102015014252.6A external-priority patent/DE102015014252A1/de
Application filed by Kws Saat Se & Co Kgaa filed Critical Kws Saat Se & Co Kgaa
Publication of BR112017024649A2 publication Critical patent/BR112017024649A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/04—Stems
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

a presente invenção refere-se a métodos de transformação in planta para plantas ou materiais vegetais com um constructo genético, em que pelo menos uma célula meristemática de um tecido meristemático de uma inflorescência imatura capaz de se diferenciar em um gameta de um pólen ou de um óvulo é exposta e, em seguida, transformada, em que a transformação pode ser realizada para gerar uma integração estável ou para gerar uma introdução transiente de um constructo genético de interesse. são ainda providos métodos para fabricação de uma planta transgênica ou métodos para fabricação de uma planta geneticamente manipulada com base nos métodos de transformação in planta de acordo com a presente invenção. além disso, é provida uma planta ou uma progênie desta fabricada de acordo com os métodos de transformação e/ou fabricação de acordo com a presente invenção.
BR112017024649A 2015-05-19 2016-05-19 métodos para a transformação in planta de plantas e processos de fabricação e produtos com base nelas e obteníveis a partir delas BR112017024649A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE102015006335.9A DE102015006335A1 (de) 2015-05-19 2015-05-19 Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen
DE102015014252.6A DE102015014252A1 (de) 2015-11-05 2015-11-05 Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen
EP15202060.8A EP3095870A1 (en) 2015-05-19 2015-12-22 Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
PCT/EP2016/061338 WO2016184989A1 (en) 2015-05-19 2016-05-19 Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom

Publications (1)

Publication Number Publication Date
BR112017024649A2 true BR112017024649A2 (pt) 2018-07-24

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
BR112017024754A BR112017024754A2 (pt) 2015-05-19 2016-05-19 método e construtos para a edição de ácido nucléico direcionada em plantas
BR112017024649A BR112017024649A2 (pt) 2015-05-19 2016-05-19 métodos para a transformação in planta de plantas e processos de fabricação e produtos com base nelas e obteníveis a partir delas

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BR112017024754A BR112017024754A2 (pt) 2015-05-19 2016-05-19 método e construtos para a edição de ácido nucléico direcionada em plantas

Country Status (10)

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US (3) US20180142248A1 (pt)
EP (3) EP3095870A1 (pt)
CN (2) CN107849582A (pt)
AU (1) AU2016265197A1 (pt)
BR (2) BR112017024754A2 (pt)
CA (2) CA2989368A1 (pt)
DK (1) DK3298148T5 (pt)
EA (1) EA201792542A1 (pt)
ES (1) ES2953919T3 (pt)
WO (2) WO2016184989A1 (pt)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3613852A3 (en) 2011-07-22 2020-04-22 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
IL258821B (en) 2015-10-23 2022-07-01 Harvard College Nucleobase editors and their uses
JP6967217B2 (ja) 2016-05-13 2021-11-17 株式会社カネカ 形質転換植物の作製方法
JP2017205104A (ja) 2016-05-13 2017-11-24 株式会社カネカ 植物のゲノム編集方法
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant
SG11201900907YA (en) 2016-08-03 2019-02-27 Harvard College Adenosine nucleobase editors and uses thereof
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
GB2573062A (en) 2016-10-14 2019-10-23 Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US11168319B1 (en) 2017-02-28 2021-11-09 Inari Agriculture Technology, Inc. Plant cell culture
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
EP3663310A4 (en) 2017-08-04 2021-08-11 Peking University TALE-RVD WITH SPECIFIC DETECTION OF A DNA BASE MODIFIED BY METHYLATION AND APPLICATION OF IT
US11624077B2 (en) 2017-08-08 2023-04-11 Peking University Gene knockout method
CN111263810A (zh) 2017-08-22 2020-06-09 纳匹基因公司 使用多核苷酸指导的核酸内切酶的细胞器基因组修饰
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
CN108004266B (zh) * 2017-12-11 2021-05-04 西北大学 一种蓝猪耳遗传转化方法
US11634722B2 (en) 2018-01-22 2023-04-25 Inari Agriculture Technology, Inc. Plant gene editing systems, methods, and compositions
EP3546582A1 (en) * 2018-03-26 2019-10-02 KWS SAAT SE & Co. KGaA Promoter activating elements
CN110343724B (zh) * 2018-04-02 2021-10-12 北京大学 用于筛选和鉴定功能性lncRNA的方法
JP6614622B2 (ja) * 2018-04-17 2019-12-04 国立大学法人名古屋大学 植物ゲノム編集方法
CN112203505A (zh) * 2018-05-24 2021-01-08 孟山都技术公司 植物基因组编辑
EP3829292A4 (en) * 2018-06-01 2022-06-08 Pioneer Hi-Bred International, Inc. BRASSICA GENOME EDITING SYSTEMS AND METHODS
CN108998469A (zh) * 2018-08-02 2018-12-14 天津大学 一种快速的水稻成熟胚原位转基因方法
CN108950047B (zh) * 2018-08-08 2020-11-13 华南农业大学 一种CsTTG1基因与茶叶茸毛生长发育线性关系的鉴定方法
CN110468147B (zh) * 2018-10-24 2021-02-09 中国农业大学 一种基于大麦条纹花叶病毒的基因编辑载体系统
CN109456990B (zh) * 2018-10-24 2022-01-07 湖南杂交水稻研究中心 一种利用基因组编辑技术提高叶绿体遗传转化效率的方法
CN113874501A (zh) * 2018-11-01 2021-12-31 中国科学院遗传与发育生物学研究所 使用碱基编辑器进行靶向诱变
US20220145312A1 (en) * 2019-02-25 2022-05-12 Syngenta Crop Protection Ag Compositions and methods for driving t1 event diversity
WO2020191153A2 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
JP7334898B2 (ja) * 2019-04-12 2023-08-29 国立大学法人東海国立大学機構 花粉への物質導入方法
EP3757219A1 (en) * 2019-06-28 2020-12-30 KWS SAAT SE & Co. KGaA Enhanced plant regeneration and transformation by using grf1 booster gene
EP3999103A4 (en) 2019-07-19 2023-11-22 Inari Agriculture Technology, Inc. GENOMIC EDITING FOR ENHANCED HOMOLOGY-DIRECTED REPAIR
WO2021046154A1 (en) * 2019-09-06 2021-03-11 James Berman Genetic modification of plants
CN112779266A (zh) * 2019-11-06 2021-05-11 青岛清原化合物有限公司 在生物体内创制新基因的方法及应用
CA3157622A1 (en) * 2019-11-26 2021-06-03 Heng Zhong Methods of transformation
CN115087739A (zh) * 2020-01-03 2022-09-20 株式会社钟化 植物的修饰方法
US20230081632A1 (en) * 2020-02-28 2023-03-16 KWS SAAT SE & Co. KGaA Immature inflorescence meristem editing
WO2021170787A1 (en) * 2020-02-28 2021-09-02 KWS SAAT SE & Co. KGaA Method for rapid genome modification in recalcitrant plants
CN111454984A (zh) * 2020-03-04 2020-07-28 深圳大学 一种植物基因转化方法及其应用
KR20230019843A (ko) 2020-05-08 2023-02-09 더 브로드 인스티튜트, 인코퍼레이티드 표적 이중 가닥 뉴클레오티드 서열의 두 가닥의 동시 편집을 위한 방법 및 조성물
CA3191387A1 (en) 2020-09-30 2022-04-07 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
JP7345760B2 (ja) * 2020-10-07 2023-09-19 国立大学法人東海国立大学機構 花粉への物質導入方法
WO2022226107A1 (en) * 2021-04-20 2022-10-27 Texas Tech University System Tissue-culture independent gene editing of cells by a long-distance rna transport system
CN113234720B (zh) * 2021-04-22 2023-08-18 河南城建学院 小麦长链非编码RNAlncR156及其在调控小麦响应干旱胁迫中的应用
CN113693086A (zh) * 2021-09-08 2021-11-26 山西省农业科学院园艺研究所 一种鸢尾植株专用抗旱剂
CN114375716A (zh) * 2022-01-19 2022-04-22 青海省农林科学院 一种青稞条纹病菌液体侵染鉴定方法
WO2023147222A1 (en) 2022-01-25 2023-08-03 Living Carbon PBC Compositions and methods for enhancing biomass productivity in plants
WO2023172875A1 (en) * 2022-03-07 2023-09-14 Inedita Bio, Inc. Methods and compositions for intron mediated- expression of regulatory elements for trait development
WO2024030442A1 (en) * 2022-08-01 2024-02-08 Purdue Research Foundation Efficient genotype-independent in planta transformation of cereals
WO2024054536A2 (en) * 2022-09-08 2024-03-14 Paul Westmeyer Nanoscopic projectiles
CN116584376A (zh) * 2023-06-28 2023-08-15 湖北省农业科学院经济作物研究所 一种桑树快速杂交育种方法

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1288073C (en) * 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US5910631A (en) 1993-09-03 1999-06-08 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Middle chain-specific thioesterase genes from Cuphea lanceolata
US5736369A (en) * 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US6583335B1 (en) 1997-04-18 2003-06-24 Texas Tech University Direct transformation of higher plants through pollen tube pathway
AU760113C (en) 1997-11-18 2004-04-22 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
MXPA01010930A (es) 1999-04-29 2003-06-30 Syngenta Ltd Plantas resistentes a herbicidas.
US6846970B1 (en) 1999-07-19 2005-01-25 Plant Bioscience Limited Transformation method and transgenic plants produced thereby
US6603061B1 (en) 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
JP2004528808A (ja) 2000-09-29 2004-09-24 シンジェンタ リミテッド 除草剤抵抗性植物
US20030135891A1 (en) 2001-12-04 2003-07-17 The Texas A&M University System Method of transforming intact plants
AU2003247962B2 (en) 2002-07-18 2008-06-12 Monsanto Technology Llc Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
CN1175724C (zh) * 2002-11-28 2004-11-17 上海交通大学 超声波辅助大豆原位丛生芽转化的方法
US20050039228A1 (en) * 2003-06-19 2005-02-17 The Samuel Roberts Noble Foundation Methods and compositions for analysis of plant gene function
CN1286978C (zh) * 2004-11-08 2006-11-29 安徽农业大学 简便高效的农杆菌介导玉米转基因方法
CN100451122C (zh) * 2006-03-07 2009-01-14 中国农业科学院油料作物研究所 油菜转基因的方法
DK2205749T3 (en) 2007-09-27 2016-08-22 Dow Agrosciences Llc MODIFIED PROTEINS zinc finger, which target the 5-enolpyruvylshikimate-3-phosphate synthase genes
JP2011120478A (ja) * 2008-03-31 2011-06-23 Japan Tobacco Inc アグロバクテリウム菌による形質転換植物の作成方法
EP2510096B2 (en) 2009-12-10 2018-02-07 Regents of the University of Minnesota Tal effector-mediated dna modification
CN102812034B (zh) 2010-01-22 2016-08-03 陶氏益农公司 靶向基因组改造
CN103842511A (zh) 2011-03-23 2014-06-04 先锋国际良种公司 产生复合转基因性状基因座的方法
US20130145488A1 (en) 2011-12-06 2013-06-06 Iowa State University Research Foundation, Inc. Mesoporous silica nanoparticles suitable for co-delivery
WO2013096567A2 (en) 2011-12-21 2013-06-27 Duke University The hsf-like transcription factor, tbf1, is a major molecular switch for growth-to-defense transition in plants
CN102533851A (zh) * 2012-01-31 2012-07-04 安徽农业大学 一种高通量农杆菌介导的玉米基因原位转化方法
CN102558309B (zh) 2012-02-10 2014-09-17 浙江大学 一对转录激活子样效应因子核酸酶及其编码基因与应用
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
US9523098B2 (en) 2012-05-02 2016-12-20 Dow Agrosciences Llc Targeted modification of malate dehydrogenase
WO2013169802A1 (en) 2012-05-07 2013-11-14 Sangamo Biosciences, Inc. Methods and compositions for nuclease-mediated targeted integration of transgenes
PE20150336A1 (es) 2012-05-25 2015-03-25 Univ California Metodos y composiciones para la modificacion de adn objetivo dirigida por arn y para la modulacion de la transcripcion dirigida por arn
EP3808844A1 (en) 2012-07-25 2021-04-21 The Broad Institute, Inc. Inducible dna binding proteins and genome perturbation tools and applications thereof
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
US20140096284A1 (en) 2012-10-01 2014-04-03 Iowa State University Research Foundation, Inc. Method for the delivery of molecules lyophilized onto microparticles to plant tissues
CN116064533A (zh) 2012-10-23 2023-05-05 基因工具股份有限公司 用于切割靶dna的组合物及其用途
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
CN103898099B (zh) 2012-12-25 2017-08-25 袁晶 一种实现基因组定点修饰的蛋白表达载体的方法
ES2705033T3 (es) 2012-12-27 2019-03-21 Keygene Nv Método para eliminar un ligamiento genético en una planta
WO2014130955A1 (en) * 2013-02-25 2014-08-28 Sangamo Biosciences, Inc. Methods and compositions for enhancing nuclease-mediated gene disruption
US20140273235A1 (en) 2013-03-15 2014-09-18 Regents Of The University Of Minnesota ENGINEERING PLANT GENOMES USING CRISPR/Cas SYSTEMS
CN105121650A (zh) 2013-04-02 2015-12-02 拜尔作物科学公司 真核生物中的靶向基因组工程
WO2014194190A1 (en) * 2013-05-30 2014-12-04 The Penn State Research Foundation Gene targeting and genetic modification of plants via rna-guided genome editing
AU2014279694B2 (en) 2013-06-14 2020-07-23 Cellectis Methods for non-transgenic genome editing in plants
CN103343120B (zh) 2013-07-04 2015-03-04 中国科学院遗传与发育生物学研究所 一种小麦基因组定点改造方法
CN103382468B (zh) 2013-07-04 2015-04-29 中国科学院遗传与发育生物学研究所 一种水稻基因组定点改造方法
US20160264982A1 (en) 2013-07-16 2016-09-15 Shanghai Institutes For Biological Sciences, Chinese Academy Of Sciences Method for plant genome site-directed modification
CN103555711A (zh) 2013-07-22 2014-02-05 安徽省农业科学院水稻研究所 一种主要农作物非转基因的基因组定向分子改良方法和应用
LT3036327T (lt) * 2013-08-22 2019-06-25 Pioneer Hi-Bred International, Inc. Genomo modifikacijos, panaudojant nukreipiančias polinukleotido/cas endonukleazės sistemas, ir panaudojimo būdai
BR102014027448A8 (pt) 2013-11-04 2021-08-24 Dow Agrosciences Llc cassete de doador de polinucleotídeo, uso de uma planta, de uma parte de planta, ou de uma célula vegetal compreendendo o referido cassete, e método para integração direcionada do mesmo
US10787684B2 (en) 2013-11-19 2020-09-29 President And Fellows Of Harvard College Large gene excision and insertion
CN103667338B (zh) 2013-11-28 2016-01-27 中国科学院遗传与发育生物学研究所 一种玉米基因组定点改造方法
CN103952405B (zh) 2014-05-13 2016-02-10 扬州大学 一种山羊mstn基因定点修饰系统及其应用
CN104212778A (zh) 2014-06-26 2014-12-17 绍兴市人民医院 基于TALEN和pMD18载体的定点突变系统及应用
CN113789317B (zh) 2014-08-06 2024-02-23 基因工具股份有限公司 使用空肠弯曲杆菌crispr/cas系统衍生的rna引导的工程化核酸酶的基因编辑
CN104293827A (zh) * 2014-09-24 2015-01-21 华南农业大学 一种定点突变RNase ZS1获得温敏不育系的方法
CA2968808A1 (en) 2014-11-27 2016-06-02 Danziger Innovations Ltd. Nucleic acid constructs for genome editing
DE102015004187A1 (de) 2015-04-02 2016-10-06 Kws Saat Se Männlich sterile Pflanze der Gattung Triticum
CN105087641A (zh) * 2015-07-31 2015-11-25 贵州省园艺研究所 一种新的农杆菌介导的大白菜原位转基因方法
CN106811479B (zh) 2015-11-30 2019-10-25 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用

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