BR112017022806A2 - método e sistema para contagem automatizada de colônias microbianas a partir de amostras semeadas em meio plaqueado - Google Patents

método e sistema para contagem automatizada de colônias microbianas a partir de amostras semeadas em meio plaqueado

Info

Publication number
BR112017022806A2
BR112017022806A2 BR112017022806-8A BR112017022806A BR112017022806A2 BR 112017022806 A2 BR112017022806 A2 BR 112017022806A2 BR 112017022806 A BR112017022806 A BR 112017022806A BR 112017022806 A2 BR112017022806 A2 BR 112017022806A2
Authority
BR
Brazil
Prior art keywords
microbial colonies
colony
automated counting
plated samples
over time
Prior art date
Application number
BR112017022806-8A
Other languages
English (en)
Inventor
M. Wiles Timothy
Rodolphe Marcelpoil Raphael
Original Assignee
Bd Kiestra B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bd Kiestra B.V. filed Critical Bd Kiestra B.V.
Publication of BR112017022806A2 publication Critical patent/BR112017022806A2/pt

Links

Classifications

    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G06T7/0014—Biomedical image inspection using an image reference approach
    • G06T7/0016—Biomedical image inspection using an image reference approach involving temporal comparison
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30242—Counting objects in image

Abstract

trata-se de um sistema de imageamento e método para detecção de crescimento microbiano, contagem ou identificação. uma colônia pode ser contrastada em uma imagem que não seja ideal para outro tipo de colônia. o sistema e método proporcionam contraste a partir de todo o material disponível através do espaço (diferenças espaciais), tempo (diferenças que surgem com o passar do tempo para uma dada condição de captura) e transformação de espaço de cor usando informações de entrada de imagem com o passar do tempo para avaliar se o crescimento microbiano ocorreu para uma dada amostra.
BR112017022806-8A 2015-04-23 2016-04-22 método e sistema para contagem automatizada de colônias microbianas a partir de amostras semeadas em meio plaqueado BR112017022806A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562151688P 2015-04-23 2015-04-23
US62/151,688 2015-04-23
US201662318488P 2016-04-05 2016-04-05
US62/318,488 2016-04-05
PCT/US2016/028919 WO2016172532A2 (en) 2015-04-23 2016-04-22 A method and system for automated microbial colony counting from streaked sample on plated media

Publications (1)

Publication Number Publication Date
BR112017022806A2 true BR112017022806A2 (pt) 2018-07-10

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BR112017022806-8A BR112017022806A2 (pt) 2015-04-23 2016-04-22 método e sistema para contagem automatizada de colônias microbianas a partir de amostras semeadas em meio plaqueado

Country Status (10)

Country Link
US (3) US10696938B2 (pt)
EP (1) EP3286732A2 (pt)
JP (2) JP6845221B2 (pt)
KR (1) KR102643990B1 (pt)
CN (2) CN107580715B (pt)
AU (2) AU2016250792B2 (pt)
BR (1) BR112017022806A2 (pt)
CA (2) CA2985854C (pt)
WO (1) WO2016172532A2 (pt)
ZA (1) ZA201707705B (pt)

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ZA201707705B (en) 2021-05-26
JP7149360B2 (ja) 2022-10-06
US10696938B2 (en) 2020-06-30
US11674116B2 (en) 2023-06-13
CN107580715B (zh) 2022-05-31
JP6845221B2 (ja) 2021-03-17
AU2016250792B2 (en) 2021-02-04
AU2016250792A1 (en) 2017-11-16
WO2016172532A3 (en) 2016-12-08
CA2985854C (en) 2023-10-17
EP3286732A2 (en) 2018-02-28
CA3211036A1 (en) 2016-10-27
JP2021090437A (ja) 2021-06-17
CA2985854A1 (en) 2016-10-27
KR102643990B1 (ko) 2024-03-05
JP2018522572A (ja) 2018-08-16
US20230383232A1 (en) 2023-11-30
AU2021202750A1 (en) 2021-05-27
US20200283719A1 (en) 2020-09-10
CN114723741A (zh) 2022-07-08
KR20170139591A (ko) 2017-12-19
US20180112173A1 (en) 2018-04-26
AU2021202750B2 (en) 2023-11-09
CN107580715A (zh) 2018-01-12
WO2016172532A2 (en) 2016-10-27

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