BR0003066A - Método e dispositivo para detecção microrganismos à fibra óptica - Google Patents

Método e dispositivo para detecção microrganismos à fibra óptica

Info

Publication number
BR0003066A
BR0003066A BR0003066-0A BR0003066A BR0003066A BR 0003066 A BR0003066 A BR 0003066A BR 0003066 A BR0003066 A BR 0003066A BR 0003066 A BR0003066 A BR 0003066A
Authority
BR
Brazil
Prior art keywords
microorganisms
optical fiber
evanescent field
sample
detected
Prior art date
Application number
BR0003066-0A
Other languages
English (en)
Inventor
Aldo Pacheco Ferreira
Ricardo Marques Ribeiro
Marcelo Martins Werneck
Original Assignee
Fundacao Oswaldo Cruz
Univ Rio De Janeiro
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fundacao Oswaldo Cruz, Univ Rio De Janeiro filed Critical Fundacao Oswaldo Cruz
Priority to BR0003066-0A priority Critical patent/BR0003066A/pt
Priority to US09/629,830 priority patent/US6718077B1/en
Publication of BR0003066A publication Critical patent/BR0003066A/pt
Priority to US10/721,510 priority patent/US20040076358A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7776—Index
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7779—Measurement method of reaction-produced change in sensor interferometric
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/26—Optical coupling means
    • G02B6/27—Optical coupling means with polarisation selective and adjusting means
    • G02B6/2726—Optical coupling means with polarisation selective and adjusting means in or on light guides, e.g. polarisation means assembled in a light guide
    • G02B6/274—Optical coupling means with polarisation selective and adjusting means in or on light guides, e.g. polarisation means assembled in a light guide based on light guide birefringence, e.g. due to coupling between light guides
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/26—Optical coupling means
    • G02B6/28—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals
    • G02B6/293—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals with wavelength selective means
    • G02B6/29346—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals with wavelength selective means operating by wave or beam interference
    • G02B6/29349—Michelson or Michelson/Gires-Tournois configuration, i.e. based on splitting and interferometrically combining relatively delayed signals at a single beamsplitter
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/26—Optical coupling means
    • G02B6/28—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals
    • G02B6/293—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals with wavelength selective means
    • G02B6/29346—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals with wavelength selective means operating by wave or beam interference
    • G02B6/2935—Mach-Zehnder configuration, i.e. comprising separate splitting and combining means
    • G02B6/29352—Mach-Zehnder configuration, i.e. comprising separate splitting and combining means in a light guide
    • G02B6/29353—Mach-Zehnder configuration, i.e. comprising separate splitting and combining means in a light guide with a wavelength selective element in at least one light guide interferometer arm, e.g. grating, interference filter, resonator
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/26—Optical coupling means
    • G02B6/28—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals
    • G02B6/293—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals with wavelength selective means
    • G02B6/29346—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals with wavelength selective means operating by wave or beam interference
    • G02B6/29358—Multiple beam interferometer external to a light guide, e.g. Fabry-Pérot, etalon, VIPA plate, OTDL plate, continuous interferometer, parallel plate resonator

Abstract

"MéTODO E DISPOSITIVO PARA DETECçãO MICRORGANISMOS à FIBRA óPTICA". O objetivo da presente invenção é a detecção/monitoramento de microrganismos presentes no ar, água ou alimentos através do emprego de um biosensor à fibra óptica com campo evanescente. Uma primeira concretização da presente invenção diz respeito a um método para a detecção de contaminação por microrganismos específicos através da aplicação do campo evanescente de uma fibra óptica sensitiva caracterizado pelas etapas de: (a) expor o campo evanescente da fibra óptica sensitiva utilizando uma técnica apropriada baseada em propriedades físico-químicas; (b) permitir o contato íntimo do campo evanescente exposto como obtido na etapa (a) com a amostra a ser examinada, estando a dita amostra em uma forma adequada para obter a geração de um sinal óptico em resposta à presença de microrganismos na amostra; (c) demodular o sinal óptico gerado na etapa (b) e utilizar esse valor na quantificação de microrganismos através de um método apropriado. Numa segunda concretização, a invenção é dirigida a composição para uso na detecção de microrganismos caracterizado por compreender um meio de cultura seletivo para o microrganismo que se deseje detectar e reagentes capazes de alterar as propriedades do meio de forma a permitir que o índice de refração do mensurando seja melhor detectado. Numa terceira concretização a invenção refere-se a um dispositivo para o sensoriamento de microrganismos a partir da inserção de uma fibra óptica sensitiva (11), com campo evanescente adequadamente exposto, na superfície ou em um volume de um meio de cultura biológico específico (12) para o microrganismo à ser detectado compreendendo um sistema de demodulação baseado em circuito de fibras ópticas e componentes relacionados.
BR0003066-0A 2000-07-21 2000-07-21 Método e dispositivo para detecção microrganismos à fibra óptica BR0003066A (pt)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BR0003066-0A BR0003066A (pt) 2000-07-21 2000-07-21 Método e dispositivo para detecção microrganismos à fibra óptica
US09/629,830 US6718077B1 (en) 2000-07-21 2000-07-31 Method and device for the detection of microorganisms by fiber optics
US10/721,510 US20040076358A1 (en) 2000-07-21 2003-11-26 Method for the Detection of Microorganisms by Fiber Optics

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BR0003066-0A BR0003066A (pt) 2000-07-21 2000-07-21 Método e dispositivo para detecção microrganismos à fibra óptica

Publications (1)

Publication Number Publication Date
BR0003066A true BR0003066A (pt) 2002-04-30

Family

ID=3944689

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0003066-0A BR0003066A (pt) 2000-07-21 2000-07-21 Método e dispositivo para detecção microrganismos à fibra óptica

Country Status (2)

Country Link
US (2) US6718077B1 (pt)
BR (1) BR0003066A (pt)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109342327A (zh) * 2018-11-20 2019-02-15 东北大学 新型微纳高双折射Sagnac光纤生物传感器

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7700056B2 (en) * 2006-08-10 2010-04-20 American Sterilizer Company Modular decontamination system
US7919059B2 (en) * 2007-04-27 2011-04-05 American Sterilizer Company Vaporized hydrogen peroxide decontamination system with concentration adjustment mode
US8007717B2 (en) * 2007-08-14 2011-08-30 American Sterilizer Company Method and apparatus for decontaminating a region without dehumidification
US8512975B2 (en) * 2008-07-24 2013-08-20 Biomerieux, Inc. Method for detection and characterization of a microorganism in a sample using time dependent spectroscopic measurements
EP2364356B1 (en) * 2008-10-31 2019-06-19 Biomerieux, Inc Methods for detection and identification of microorganisms
FR2957150A1 (fr) * 2010-03-05 2011-09-09 Nosoco Tech Procede et appareil de detection en continu et en temps sensiblement reel de traces de microbes (bacteries, virus) ou substances dangereuses ou illicites (explosifs, drogues) dans une atmosphere
DE102011101934B4 (de) 2011-05-18 2017-07-06 Christian-Albrechts-Universität Zu Kiel Großflächiger Biofilmsensor
DE102013108189A1 (de) * 2013-07-31 2015-02-05 Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG Anordnung zur optischen Messung einer Prozessgröße und Messgerät umfassend eine solche
US10200625B2 (en) 2014-01-30 2019-02-05 Bd Kiestra B.V. System and method for image acquisition using supervised high quality imaging
AU2016250792B2 (en) 2015-04-23 2021-02-04 Bd Kiestra B.V. Method and system for automatically counting microbial colonies
EP3767586A1 (en) 2015-04-23 2021-01-20 BD Kiestra B.V. Colony contrast gathering

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4242447A (en) 1978-11-29 1980-12-30 Bioresearch Rapid detection of bacteria
US4447546A (en) 1982-08-23 1984-05-08 Myron J. Block Fluorescent immunoassay employing optical fiber in capillary tube
US4558014A (en) 1983-06-13 1985-12-10 Myron J. Block Assay apparatus and methods
GB9212302D0 (en) 1992-06-10 1992-07-22 Applied Research Systems Method for improving measurement precision in evanescent wave optical biosensor assays
US5809185A (en) * 1996-04-26 1998-09-15 Mitchell; Ralph Sensor for detecting microorganisms
US5866430A (en) * 1996-06-13 1999-02-02 Grow; Ann E. Raman optrode processes and devices for detection of chemicals and microorganisms
US6117643A (en) * 1997-11-25 2000-09-12 Ut Battelle, Llc Bioluminescent bioreporter integrated circuit
US6197576B1 (en) * 1998-05-22 2001-03-06 Gideon Eden Instrument for detection of microorganisms
EP1854873A3 (en) * 2000-01-31 2008-03-19 Matsushita Ecology Systems Co., Ltd. Microorganism detecting kit, microorganism counting apparatus, and microorganism counting process

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109342327A (zh) * 2018-11-20 2019-02-15 东北大学 新型微纳高双折射Sagnac光纤生物传感器

Also Published As

Publication number Publication date
US6718077B1 (en) 2004-04-06
US20040076358A1 (en) 2004-04-22

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B08G Application fees: restoration [chapter 8.7 patent gazette]
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