BR112016013866A2 - Dispositivo para a representação por imagens de material biológico - Google Patents

Dispositivo para a representação por imagens de material biológico

Info

Publication number
BR112016013866A2
BR112016013866A2 BR112016013866A BR112016013866A BR112016013866A2 BR 112016013866 A2 BR112016013866 A2 BR 112016013866A2 BR 112016013866 A BR112016013866 A BR 112016013866A BR 112016013866 A BR112016013866 A BR 112016013866A BR 112016013866 A2 BR112016013866 A2 BR 112016013866A2
Authority
BR
Brazil
Prior art keywords
radiation
biological material
calibration
excitation
luminescence
Prior art date
Application number
BR112016013866A
Other languages
English (en)
Other versions
BR112016013866B1 (pt
Inventor
Pannhoff Helge
Feirer Christian
Original Assignee
Euroimmun Medizinische Labordiagnostika Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Euroimmun Medizinische Labordiagnostika Ag filed Critical Euroimmun Medizinische Labordiagnostika Ag
Publication of BR112016013866A2 publication Critical patent/BR112016013866A2/pt
Publication of BR112016013866B1 publication Critical patent/BR112016013866B1/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G01N21/276—Calibration, base line adjustment, drift correction with alternation of sample and standard in optical path
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G01N21/278—Constitution of standards
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/13—Standards, constitution

Abstract

DISPOSITIVO PARA A REPRESENTAÇÃO POR IMAGENS DE MATERIAL BIOLÓGICO. A presente invenção se refere a um padrão de calibração (7) para um dispositivo (1) para a representação por imagens de material biológico (6), que é pelo menos parcialmente excitado para a luminescência durante a análise. O dispositivo (1) apre senta uma unidade de iluminação (2) com uma fonte de radiação, pela qual pode ser emitida uma radiação de excitação (15) eletromagnética. Ainda está previsto um elemento de recepção (3), assegurando que o material biológico (6) disposto em um suporte (5) está posicionado dentro de uma trajetória de feixe da radiação de excitação (15). Ainda, o dispositivo apresenta pelo menos uma unidade de geração de imagens (4), recebendo a radiação de luminescência (16) emitida pelo material biológico (6) em função da excitação pela radiação de excitação (15) e gerando uma imagem pelo menos das regiões do material biológico (6) excitadas para a luminescência. Para a calibração, o dispositivo apresenta um padrão de calibração (7), emitindo uma radiação de calibração em função da excitação pela radiação de excitação (15), registrada pela unidade de geração de imagens (4) e sob consideração da radiação de calibração registrada pode gerar um sinal de calibração em um dispositivo de controle (18). A solução técnica descri ta é caracterizada pelo padrão de calibração (7) apresentar um alojamento (9) com uma substância (12) que pode ser excitada para a luminescência neste e que está fixamente conectado com a unidade de iluminação (2) ou o elemento de recepção (3) com o auxílio de um meio de fixação (19).
BR112016013866-0A 2013-12-18 2014-12-09 dispositivo para a representação por imagens de material biológico BR112016013866B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102013021097.6 2013-12-18
DE102013021097.6A DE102013021097A1 (de) 2013-12-18 2013-12-18 Kalibriernormal für eine Vorrichtung zur bildlichen Darstellung biologischen Materials
PCT/EP2014/003287 WO2015090534A1 (de) 2013-12-18 2014-12-09 Kalibriernormal für eine vorrichtung zur bildlichen darstellung biologischen materials

Publications (2)

Publication Number Publication Date
BR112016013866A2 true BR112016013866A2 (pt) 2017-08-08
BR112016013866B1 BR112016013866B1 (pt) 2020-12-08

Family

ID=52278544

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016013866-0A BR112016013866B1 (pt) 2013-12-18 2014-12-09 dispositivo para a representação por imagens de material biológico

Country Status (7)

Country Link
US (1) US20160313249A1 (pt)
EP (1) EP3084394A1 (pt)
JP (1) JP6695274B2 (pt)
CN (1) CN105917212B (pt)
BR (1) BR112016013866B1 (pt)
DE (1) DE102013021097A1 (pt)
WO (1) WO2015090534A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3396430B1 (en) * 2017-04-27 2023-08-16 Euroimmun Medizinische Labordiagnostika AG Optical scanning arrangement and method
IT201800004132A1 (it) * 2018-03-30 2019-09-30 St Microelectronics Srl Unita' di rilevamento di segnali fluorescenti, apparecchio di analisi di molecole che comprende tale unita' e metodo di rilevamento ed analisi dei segnali fluorescenti
DE102018122391A1 (de) * 2018-09-13 2020-03-19 Sikora Ag Vorrichtung und Verfahren zum Detektieren eines Gegenstandes
WO2020117947A1 (en) * 2018-12-04 2020-06-11 University Of Massachusetts System and methods of fluorescence microscope calibration

Family Cites Families (24)

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DE4311543A1 (de) * 1993-04-07 1994-10-13 Bayerische Motoren Werke Ag Vorrichtung zur Ermittlung der Konzentration einer Prüfflüssigkeit
DE19928056A1 (de) * 1999-06-16 2000-12-21 Friedrich Schiller Uni Jena Bu Verfahren und Testkit zur Bestimmung der Temperatur in den Einzelgefäßen von Multiküvetten mit Fluoreszenzreadern
US6471916B1 (en) * 1999-11-09 2002-10-29 Packard Instrument Company Apparatus and method for calibration of a microarray scanning system
US6635487B1 (en) * 2000-05-17 2003-10-21 Caliper Technologies Corp. Fluorescence standard for use in microfluidic instruments
AU2002247765B2 (en) 2001-03-28 2007-04-26 Clondiag Chip Technologies Gmbh Device for referencing fluorescence signals
US7416701B2 (en) * 2001-09-12 2008-08-26 Ecolab Inc. Calibrator for fluorosensor
JP2003248790A (ja) * 2002-02-25 2003-09-05 Hiroko Ishikawa 識別方法
DE20216998U1 (de) * 2002-11-05 2004-03-18 Evotec Oai Ag Kalibrierungsmittel
JP2005227183A (ja) * 2004-02-13 2005-08-25 National Agriculture & Bio-Oriented Research Organization 品質測定装置
US7480042B1 (en) * 2004-06-30 2009-01-20 Applied Biosystems Inc. Luminescence reference standards
CN1280623C (zh) * 2004-07-16 2006-10-18 北京博奥生物芯片有限责任公司 一种用于荧光仪器校准测量的校准基片及其制备方法
DE102004047593A1 (de) * 2004-09-30 2006-04-13 Carl Zeiss Jena Gmbh Referenzkörper für Fluoreszenzmessungen und Verfahren zur Herstellung desselben
DE102005049364B4 (de) * 2005-03-18 2023-05-25 BAM Bundesanstalt für Materialforschung und -prüfung Multifunktionelle Kalibriereinrichtung und Kit sowie ihre Verwendungen zur Charakterisierung von Lumineszenzmesssystemen
DE102005049365A1 (de) * 2005-03-18 2006-09-21 BAM Bundesanstalt für Materialforschung und -prüfung Kalibriereinrichtung und Farbstoffkit sowie ihre Verwendung zur Charakterisierung von Lumineszenzmesssystemen
US7817273B2 (en) * 2005-06-30 2010-10-19 Life Technologies Corporation Two-dimensional spectral imaging system
US7919328B2 (en) * 2006-03-10 2011-04-05 Corning Incorporated Fluorescent ion doped glass and method for using the fluorescent ion doped glass to enhance fluorescence imaging techniques
JP4196220B2 (ja) * 2006-10-18 2008-12-17 セイコーエプソン株式会社 液体噴射ヘッドのアライメント装置及びそのアラインメント方法
AU2009237945B2 (en) 2008-04-17 2014-11-13 Qiagen Lake Constance Gmbh Fluorescence standard, and the use thereof
US8259170B2 (en) * 2009-08-24 2012-09-04 Cellomics, Inc. Integrated calibration sample bay for fluorescence readers
DE102009041967A1 (de) * 2009-09-17 2011-03-24 Lre Medical Gmbh Vorrichtung zur Analyse von Körperflüssigkeiten
CN101979544A (zh) * 2010-11-02 2011-02-23 中国计量学院 一种基于标准样品的实时荧光pcr相对标定方法
WO2012062884A1 (de) * 2010-11-10 2012-05-18 Nath Guenther Optisches bestrahlungsgerät für dermatologie und kosmetik
DE102011079941A1 (de) * 2011-07-27 2013-01-31 Leica Microsystems Cms Gmbh Mikroskopbeleuchtungsverfahren und Mikroskop
JP2013168533A (ja) * 2012-02-16 2013-08-29 Panasonic Corp 樹脂塗布装置および樹脂塗布方法

Also Published As

Publication number Publication date
JP2017502280A (ja) 2017-01-19
JP6695274B2 (ja) 2020-05-20
US20160313249A1 (en) 2016-10-27
WO2015090534A1 (de) 2015-06-25
DE102013021097A1 (de) 2015-06-18
EP3084394A1 (de) 2016-10-26
BR112016013866B1 (pt) 2020-12-08
CN105917212A (zh) 2016-08-31
CN105917212B (zh) 2020-05-12

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 09/12/2014, OBSERVADAS AS CONDICOES LEGAIS.