BR112015022321A2 - método para purificação de uma proteína - Google Patents

método para purificação de uma proteína

Info

Publication number
BR112015022321A2
BR112015022321A2 BR112015022321A BR112015022321A BR112015022321A2 BR 112015022321 A2 BR112015022321 A2 BR 112015022321A2 BR 112015022321 A BR112015022321 A BR 112015022321A BR 112015022321 A BR112015022321 A BR 112015022321A BR 112015022321 A2 BR112015022321 A2 BR 112015022321A2
Authority
BR
Brazil
Prior art keywords
protein
superantigen
purifying
immobilized
contaminant
Prior art date
Application number
BR112015022321A
Other languages
English (en)
Portuguese (pt)
Inventor
Raul Ubiera Antonio
J Suda Eric
E Goklen Kent
Original Assignee
Glaxosmithkline Ip No 2 Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50391244&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112015022321(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Glaxosmithkline Ip No 2 Ltd filed Critical Glaxosmithkline Ip No 2 Ltd
Publication of BR112015022321A2 publication Critical patent/BR112015022321A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BR112015022321A 2013-03-15 2014-03-13 método para purificação de uma proteína BR112015022321A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361787309P 2013-03-15 2013-03-15
PCT/IB2014/059755 WO2014141150A1 (en) 2013-03-15 2014-03-13 Methods for purifying antibodies

Publications (1)

Publication Number Publication Date
BR112015022321A2 true BR112015022321A2 (pt) 2017-07-18

Family

ID=50391244

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015022321A BR112015022321A2 (pt) 2013-03-15 2014-03-13 método para purificação de uma proteína

Country Status (15)

Country Link
US (3) US10676503B2 (https=)
EP (3) EP2969098B1 (https=)
JP (2) JP6599773B2 (https=)
KR (1) KR102114021B1 (https=)
CN (1) CN105188872B (https=)
AU (1) AU2014229280B2 (https=)
BR (1) BR112015022321A2 (https=)
CA (1) CA2902287A1 (https=)
DK (2) DK3680000T3 (https=)
ES (2) ES2765206T3 (https=)
IL (1) IL240753B (https=)
RU (1) RU2651483C2 (https=)
SG (1) SG11201506494XA (https=)
TW (1) TWI625335B (https=)
WO (1) WO2014141150A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2969098B1 (en) 2013-03-15 2019-10-30 Glaxosmithkline Intellectual Property (No. 2) Limited Methods for purifying antibodies
EP2997036B1 (en) 2013-05-15 2021-03-03 Medimmune Limited Purification of recombinantly produced polypeptides
US20170044210A1 (en) 2014-04-30 2017-02-16 Novo Nordisk A/S Methods for the purification of proteins using caprylic acid
CN108064286A (zh) 2015-01-26 2018-05-22 株式会社钟化 突变型免疫球蛋白κ链可变区结合性肽
WO2016121701A1 (ja) 2015-01-26 2016-08-04 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
WO2017195638A1 (ja) * 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
RU2759909C2 (ru) * 2016-09-07 2021-11-18 Глэксосмитклайн Интеллекчуал Проперти Дивелопмент Лимитед Способы очистки антител
CN106939045B (zh) * 2017-05-01 2020-03-31 深圳市国创纳米抗体技术有限公司 一种单克隆抗体细胞培养液澄清的方法
CN108085358A (zh) * 2018-02-08 2018-05-29 保定冀中药业有限公司 一种犬细小病毒单克隆抗体IgG2b-Fab片段的制备方法
TW202003555A (zh) * 2018-03-07 2020-01-16 英商葛蘭素史克智慧財產發展有限公司 用於純化重組多肽之方法
TW202000891A (zh) 2018-03-07 2020-01-01 英商葛蘭素史克智慧財產發展有限公司 純化抗體之方法
US20230302381A1 (en) * 2020-08-18 2023-09-28 Sartorius Bia Separations D.O.O. Metal-affinity extraction of host cell dna
CN114133446A (zh) * 2021-12-31 2022-03-04 方坦思(上海)生物医药有限公司 单克隆抗体的纯化方法
CN116179582A (zh) * 2022-09-27 2023-05-30 南京医事达生物科技有限公司 一种通用型基因重组蛋白质a亲和纯化填料制备方法和应用

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57106673A (en) 1980-12-24 1982-07-02 Chugai Pharmaceut Co Ltd Dibenzo(b,f)(1,4)oxazepin derivative
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US6955917B2 (en) 1997-06-20 2005-10-18 Bayer Healthcare Llc Chromatographic method for high yield purification and viral inactivation of antibodies
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
DE60203176T2 (de) * 2001-06-01 2006-04-27 Upfront Chromatography A/S, Kopenhagen Fraktionierung von proteinhaltigen mischungen
PT1419179E (pt) 2001-08-10 2010-03-16 Univ Aberdeen Domínios de ligação de antigenes
MXPA04011769A (es) 2002-05-31 2005-07-26 Indian Agricultural Council Deteccion rapida de toxinas cry de bacillus thuringiensis.
CA2557229C (en) 2004-02-27 2012-07-10 Octapharma Ag A method of providing a purified, virus safe antibody preparation
WO2005113604A2 (en) * 2004-05-14 2005-12-01 Hematech, Llc Methods for immunoglobulin purification
WO2007109163A2 (en) * 2006-03-16 2007-09-27 Amgen Inc Wash buffer and method of using
CN101511387A (zh) 2006-09-08 2009-08-19 惠氏公司 使用亲和色谱法纯化蛋白质的精氨酸洗涤液
WO2008085988A1 (en) * 2007-01-05 2008-07-17 Amgen Inc. Methods of purifying proteins
CN107915769A (zh) * 2011-03-29 2018-04-17 葛兰素史密斯克莱有限责任公司 用于蛋白纯化的缓冲液体系
EP2969098B1 (en) * 2013-03-15 2019-10-30 Glaxosmithkline Intellectual Property (No. 2) Limited Methods for purifying antibodies
EP2997036B1 (en) * 2013-05-15 2021-03-03 Medimmune Limited Purification of recombinantly produced polypeptides
CN120904322A (zh) 2013-09-13 2025-11-07 豪夫迈·罗氏有限公司 包含纯化的重组多肽的方法和组合物

Also Published As

Publication number Publication date
JP2016519052A (ja) 2016-06-30
KR102114021B1 (ko) 2020-05-22
US10676503B2 (en) 2020-06-09
CN105188872B (zh) 2019-01-15
EP2969098B1 (en) 2019-10-30
JP6599773B2 (ja) 2019-10-30
WO2014141150A1 (en) 2014-09-18
CN105188872A (zh) 2015-12-23
EP4371996A2 (en) 2024-05-22
EP4371996A3 (en) 2024-08-14
US20200277330A1 (en) 2020-09-03
IL240753B (en) 2021-01-31
US12358945B2 (en) 2025-07-15
DK2969098T3 (da) 2020-01-27
US20250353878A1 (en) 2025-11-20
CA2902287A1 (en) 2014-09-18
US20160024146A1 (en) 2016-01-28
IL240753A0 (en) 2015-10-29
DK3680000T3 (da) 2024-04-02
EP3680000A1 (en) 2020-07-15
RU2651483C2 (ru) 2018-04-19
RU2015136860A (ru) 2017-04-28
EP3680000B1 (en) 2024-03-13
TW201522365A (zh) 2015-06-16
JP2020019795A (ja) 2020-02-06
AU2014229280A1 (en) 2015-10-01
KR20150131235A (ko) 2015-11-24
AU2014229280B2 (en) 2017-01-05
TWI625335B (zh) 2018-06-01
ES2978309T3 (es) 2024-09-10
JP7002511B2 (ja) 2022-02-21
EP2969098A1 (en) 2016-01-20
SG11201506494XA (en) 2015-09-29
ES2765206T3 (es) 2020-06-08

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements