ES2765206T3 - Procedimientos de purificación de anticuerpos - Google Patents
Procedimientos de purificación de anticuerpos Download PDFInfo
- Publication number
- ES2765206T3 ES2765206T3 ES14713926T ES14713926T ES2765206T3 ES 2765206 T3 ES2765206 T3 ES 2765206T3 ES 14713926 T ES14713926 T ES 14713926T ES 14713926 T ES14713926 T ES 14713926T ES 2765206 T3 ES2765206 T3 ES 2765206T3
- Authority
- ES
- Spain
- Prior art keywords
- protein
- acid
- sodium
- wash buffer
- host cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361787309P | 2013-03-15 | 2013-03-15 | |
| PCT/IB2014/059755 WO2014141150A1 (en) | 2013-03-15 | 2014-03-13 | Methods for purifying antibodies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2765206T3 true ES2765206T3 (es) | 2020-06-08 |
Family
ID=50391244
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES14713926T Active ES2765206T3 (es) | 2013-03-15 | 2014-03-13 | Procedimientos de purificación de anticuerpos |
| ES19198005T Active ES2978309T3 (es) | 2013-03-15 | 2014-03-13 | Métodos para purificar anticuerpos |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES19198005T Active ES2978309T3 (es) | 2013-03-15 | 2014-03-13 | Métodos para purificar anticuerpos |
Country Status (15)
| Country | Link |
|---|---|
| US (3) | US10676503B2 (https=) |
| EP (3) | EP2969098B1 (https=) |
| JP (2) | JP6599773B2 (https=) |
| KR (1) | KR102114021B1 (https=) |
| CN (1) | CN105188872B (https=) |
| AU (1) | AU2014229280B2 (https=) |
| BR (1) | BR112015022321A2 (https=) |
| CA (1) | CA2902287A1 (https=) |
| DK (2) | DK3680000T3 (https=) |
| ES (2) | ES2765206T3 (https=) |
| IL (1) | IL240753B (https=) |
| RU (1) | RU2651483C2 (https=) |
| SG (1) | SG11201506494XA (https=) |
| TW (1) | TWI625335B (https=) |
| WO (1) | WO2014141150A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2969098B1 (en) | 2013-03-15 | 2019-10-30 | Glaxosmithkline Intellectual Property (No. 2) Limited | Methods for purifying antibodies |
| EP2997036B1 (en) | 2013-05-15 | 2021-03-03 | Medimmune Limited | Purification of recombinantly produced polypeptides |
| US20170044210A1 (en) | 2014-04-30 | 2017-02-16 | Novo Nordisk A/S | Methods for the purification of proteins using caprylic acid |
| CN108064286A (zh) | 2015-01-26 | 2018-05-22 | 株式会社钟化 | 突变型免疫球蛋白κ链可变区结合性肽 |
| WO2016121701A1 (ja) | 2015-01-26 | 2016-08-04 | 株式会社カネカ | 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス |
| WO2017195638A1 (ja) * | 2016-05-09 | 2017-11-16 | 株式会社カネカ | 抗体またはκ鎖可変領域含有抗体断片の精製方法 |
| RU2759909C2 (ru) * | 2016-09-07 | 2021-11-18 | Глэксосмитклайн Интеллекчуал Проперти Дивелопмент Лимитед | Способы очистки антител |
| CN106939045B (zh) * | 2017-05-01 | 2020-03-31 | 深圳市国创纳米抗体技术有限公司 | 一种单克隆抗体细胞培养液澄清的方法 |
| CN108085358A (zh) * | 2018-02-08 | 2018-05-29 | 保定冀中药业有限公司 | 一种犬细小病毒单克隆抗体IgG2b-Fab片段的制备方法 |
| TW202003555A (zh) * | 2018-03-07 | 2020-01-16 | 英商葛蘭素史克智慧財產發展有限公司 | 用於純化重組多肽之方法 |
| TW202000891A (zh) | 2018-03-07 | 2020-01-01 | 英商葛蘭素史克智慧財產發展有限公司 | 純化抗體之方法 |
| US20230302381A1 (en) * | 2020-08-18 | 2023-09-28 | Sartorius Bia Separations D.O.O. | Metal-affinity extraction of host cell dna |
| CN114133446A (zh) * | 2021-12-31 | 2022-03-04 | 方坦思(上海)生物医药有限公司 | 单克隆抗体的纯化方法 |
| CN116179582A (zh) * | 2022-09-27 | 2023-05-30 | 南京医事达生物科技有限公司 | 一种通用型基因重组蛋白质a亲和纯化填料制备方法和应用 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57106673A (en) | 1980-12-24 | 1982-07-02 | Chugai Pharmaceut Co Ltd | Dibenzo(b,f)(1,4)oxazepin derivative |
| GB8607679D0 (en) | 1986-03-27 | 1986-04-30 | Winter G P | Recombinant dna product |
| US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| US6955917B2 (en) | 1997-06-20 | 2005-10-18 | Bayer Healthcare Llc | Chromatographic method for high yield purification and viral inactivation of antibodies |
| IL127127A0 (en) | 1998-11-18 | 1999-09-22 | Peptor Ltd | Small functional units of antibody heavy chain variable regions |
| DE60203176T2 (de) * | 2001-06-01 | 2006-04-27 | Upfront Chromatography A/S, Kopenhagen | Fraktionierung von proteinhaltigen mischungen |
| PT1419179E (pt) | 2001-08-10 | 2010-03-16 | Univ Aberdeen | Domínios de ligação de antigenes |
| MXPA04011769A (es) | 2002-05-31 | 2005-07-26 | Indian Agricultural Council | Deteccion rapida de toxinas cry de bacillus thuringiensis. |
| CA2557229C (en) | 2004-02-27 | 2012-07-10 | Octapharma Ag | A method of providing a purified, virus safe antibody preparation |
| WO2005113604A2 (en) * | 2004-05-14 | 2005-12-01 | Hematech, Llc | Methods for immunoglobulin purification |
| WO2007109163A2 (en) * | 2006-03-16 | 2007-09-27 | Amgen Inc | Wash buffer and method of using |
| CN101511387A (zh) | 2006-09-08 | 2009-08-19 | 惠氏公司 | 使用亲和色谱法纯化蛋白质的精氨酸洗涤液 |
| WO2008085988A1 (en) * | 2007-01-05 | 2008-07-17 | Amgen Inc. | Methods of purifying proteins |
| CN107915769A (zh) * | 2011-03-29 | 2018-04-17 | 葛兰素史密斯克莱有限责任公司 | 用于蛋白纯化的缓冲液体系 |
| EP2969098B1 (en) * | 2013-03-15 | 2019-10-30 | Glaxosmithkline Intellectual Property (No. 2) Limited | Methods for purifying antibodies |
| EP2997036B1 (en) * | 2013-05-15 | 2021-03-03 | Medimmune Limited | Purification of recombinantly produced polypeptides |
| CN120904322A (zh) | 2013-09-13 | 2025-11-07 | 豪夫迈·罗氏有限公司 | 包含纯化的重组多肽的方法和组合物 |
-
2014
- 2014-03-13 EP EP14713926.5A patent/EP2969098B1/en active Active
- 2014-03-13 BR BR112015022321A patent/BR112015022321A2/pt not_active Application Discontinuation
- 2014-03-13 EP EP24155364.3A patent/EP4371996A3/en active Pending
- 2014-03-13 AU AU2014229280A patent/AU2014229280B2/en not_active Ceased
- 2014-03-13 CN CN201480016016.XA patent/CN105188872B/zh active Active
- 2014-03-13 DK DK19198005.1T patent/DK3680000T3/da active
- 2014-03-13 KR KR1020157029042A patent/KR102114021B1/ko active Active
- 2014-03-13 RU RU2015136860A patent/RU2651483C2/ru active
- 2014-03-13 TW TW103108898A patent/TWI625335B/zh active
- 2014-03-13 WO PCT/IB2014/059755 patent/WO2014141150A1/en not_active Ceased
- 2014-03-13 ES ES14713926T patent/ES2765206T3/es active Active
- 2014-03-13 DK DK14713926.5T patent/DK2969098T3/da active
- 2014-03-13 US US14/775,868 patent/US10676503B2/en active Active
- 2014-03-13 EP EP19198005.1A patent/EP3680000B1/en active Active
- 2014-03-13 CA CA2902287A patent/CA2902287A1/en not_active Abandoned
- 2014-03-13 JP JP2015562513A patent/JP6599773B2/ja active Active
- 2014-03-13 ES ES19198005T patent/ES2978309T3/es active Active
- 2014-03-13 SG SG11201506494XA patent/SG11201506494XA/en unknown
-
2015
- 2015-08-23 IL IL240753A patent/IL240753B/en active IP Right Grant
-
2019
- 2019-10-03 JP JP2019182562A patent/JP7002511B2/ja active Active
-
2020
- 2020-05-13 US US15/931,062 patent/US12358945B2/en active Active
-
2025
- 2025-06-12 US US19/235,815 patent/US20250353878A1/en active Pending
Also Published As
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| JP7058272B2 (ja) | 抗体を精製するための方法 | |
| CN102712673B (zh) | 用于纯化含fc的蛋白的色谱方法 |