KR102114021B1 - 항체를 정제하는 방법 - Google Patents

항체를 정제하는 방법 Download PDF

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Publication number
KR102114021B1
KR102114021B1 KR1020157029042A KR20157029042A KR102114021B1 KR 102114021 B1 KR102114021 B1 KR 102114021B1 KR 1020157029042 A KR1020157029042 A KR 1020157029042A KR 20157029042 A KR20157029042 A KR 20157029042A KR 102114021 B1 KR102114021 B1 KR 102114021B1
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protein
acid
delete delete
sodium
wash buffer
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Korean (ko)
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KR20150131235A (ko
Inventor
켄트 이. 고크렌
에릭 제이. 수다
안토니오 라울 우비에라
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글락소스미스클라인 인털렉츄얼 프로퍼티 (넘버 2) 리미티드
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Application filed by 글락소스미스클라인 인털렉츄얼 프로퍼티 (넘버 2) 리미티드 filed Critical 글락소스미스클라인 인털렉츄얼 프로퍼티 (넘버 2) 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020157029042A 2013-03-15 2014-03-13 항체를 정제하는 방법 Active KR102114021B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361787309P 2013-03-15 2013-03-15
US61/787,309 2013-03-15
PCT/IB2014/059755 WO2014141150A1 (en) 2013-03-15 2014-03-13 Methods for purifying antibodies

Publications (2)

Publication Number Publication Date
KR20150131235A KR20150131235A (ko) 2015-11-24
KR102114021B1 true KR102114021B1 (ko) 2020-05-22

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ID=50391244

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KR1020157029042A Active KR102114021B1 (ko) 2013-03-15 2014-03-13 항체를 정제하는 방법

Country Status (15)

Country Link
US (3) US10676503B2 (https=)
EP (3) EP2969098B1 (https=)
JP (2) JP6599773B2 (https=)
KR (1) KR102114021B1 (https=)
CN (1) CN105188872B (https=)
AU (1) AU2014229280B2 (https=)
BR (1) BR112015022321A2 (https=)
CA (1) CA2902287A1 (https=)
DK (2) DK3680000T3 (https=)
ES (2) ES2765206T3 (https=)
IL (1) IL240753B (https=)
RU (1) RU2651483C2 (https=)
SG (1) SG11201506494XA (https=)
TW (1) TWI625335B (https=)
WO (1) WO2014141150A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2969098B1 (en) 2013-03-15 2019-10-30 Glaxosmithkline Intellectual Property (No. 2) Limited Methods for purifying antibodies
EP2997036B1 (en) 2013-05-15 2021-03-03 Medimmune Limited Purification of recombinantly produced polypeptides
US20170044210A1 (en) 2014-04-30 2017-02-16 Novo Nordisk A/S Methods for the purification of proteins using caprylic acid
CN108064286A (zh) 2015-01-26 2018-05-22 株式会社钟化 突变型免疫球蛋白κ链可变区结合性肽
WO2016121701A1 (ja) 2015-01-26 2016-08-04 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
WO2017195638A1 (ja) * 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
RU2759909C2 (ru) * 2016-09-07 2021-11-18 Глэксосмитклайн Интеллекчуал Проперти Дивелопмент Лимитед Способы очистки антител
CN106939045B (zh) * 2017-05-01 2020-03-31 深圳市国创纳米抗体技术有限公司 一种单克隆抗体细胞培养液澄清的方法
CN108085358A (zh) * 2018-02-08 2018-05-29 保定冀中药业有限公司 一种犬细小病毒单克隆抗体IgG2b-Fab片段的制备方法
TW202003555A (zh) * 2018-03-07 2020-01-16 英商葛蘭素史克智慧財產發展有限公司 用於純化重組多肽之方法
TW202000891A (zh) 2018-03-07 2020-01-01 英商葛蘭素史克智慧財產發展有限公司 純化抗體之方法
US20230302381A1 (en) * 2020-08-18 2023-09-28 Sartorius Bia Separations D.O.O. Metal-affinity extraction of host cell dna
CN114133446A (zh) * 2021-12-31 2022-03-04 方坦思(上海)生物医药有限公司 单克隆抗体的纯化方法
CN116179582A (zh) * 2022-09-27 2023-05-30 南京医事达生物科技有限公司 一种通用型基因重组蛋白质a亲和纯化填料制备方法和应用

Citations (2)

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WO2005082937A2 (en) 2004-02-27 2005-09-09 Octapharma Ag A method of providing a purified, virus safe antibody preparation
WO2007109163A2 (en) * 2006-03-16 2007-09-27 Amgen Inc Wash buffer and method of using

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JPS57106673A (en) 1980-12-24 1982-07-02 Chugai Pharmaceut Co Ltd Dibenzo(b,f)(1,4)oxazepin derivative
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US6955917B2 (en) 1997-06-20 2005-10-18 Bayer Healthcare Llc Chromatographic method for high yield purification and viral inactivation of antibodies
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
DE60203176T2 (de) * 2001-06-01 2006-04-27 Upfront Chromatography A/S, Kopenhagen Fraktionierung von proteinhaltigen mischungen
PT1419179E (pt) 2001-08-10 2010-03-16 Univ Aberdeen Domínios de ligação de antigenes
MXPA04011769A (es) 2002-05-31 2005-07-26 Indian Agricultural Council Deteccion rapida de toxinas cry de bacillus thuringiensis.
WO2005113604A2 (en) * 2004-05-14 2005-12-01 Hematech, Llc Methods for immunoglobulin purification
CN101511387A (zh) 2006-09-08 2009-08-19 惠氏公司 使用亲和色谱法纯化蛋白质的精氨酸洗涤液
WO2008085988A1 (en) * 2007-01-05 2008-07-17 Amgen Inc. Methods of purifying proteins
CN107915769A (zh) * 2011-03-29 2018-04-17 葛兰素史密斯克莱有限责任公司 用于蛋白纯化的缓冲液体系
EP2969098B1 (en) * 2013-03-15 2019-10-30 Glaxosmithkline Intellectual Property (No. 2) Limited Methods for purifying antibodies
EP2997036B1 (en) * 2013-05-15 2021-03-03 Medimmune Limited Purification of recombinantly produced polypeptides
CN120904322A (zh) 2013-09-13 2025-11-07 豪夫迈·罗氏有限公司 包含纯化的重组多肽的方法和组合物

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005082937A2 (en) 2004-02-27 2005-09-09 Octapharma Ag A method of providing a purified, virus safe antibody preparation
WO2007109163A2 (en) * 2006-03-16 2007-09-27 Amgen Inc Wash buffer and method of using

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Biotechnol Bioeng.,109(10):2589-2598(2012.10.)
J Chromatogr B Analyt Technol Biomed Life Sci.,860(2):209-217(2007.11.19)*

Also Published As

Publication number Publication date
JP2016519052A (ja) 2016-06-30
US10676503B2 (en) 2020-06-09
CN105188872B (zh) 2019-01-15
EP2969098B1 (en) 2019-10-30
JP6599773B2 (ja) 2019-10-30
WO2014141150A1 (en) 2014-09-18
BR112015022321A2 (pt) 2017-07-18
CN105188872A (zh) 2015-12-23
EP4371996A2 (en) 2024-05-22
EP4371996A3 (en) 2024-08-14
US20200277330A1 (en) 2020-09-03
IL240753B (en) 2021-01-31
US12358945B2 (en) 2025-07-15
DK2969098T3 (da) 2020-01-27
US20250353878A1 (en) 2025-11-20
CA2902287A1 (en) 2014-09-18
US20160024146A1 (en) 2016-01-28
IL240753A0 (en) 2015-10-29
DK3680000T3 (da) 2024-04-02
EP3680000A1 (en) 2020-07-15
RU2651483C2 (ru) 2018-04-19
RU2015136860A (ru) 2017-04-28
EP3680000B1 (en) 2024-03-13
TW201522365A (zh) 2015-06-16
JP2020019795A (ja) 2020-02-06
AU2014229280A1 (en) 2015-10-01
KR20150131235A (ko) 2015-11-24
AU2014229280B2 (en) 2017-01-05
TWI625335B (zh) 2018-06-01
ES2978309T3 (es) 2024-09-10
JP7002511B2 (ja) 2022-02-21
EP2969098A1 (en) 2016-01-20
SG11201506494XA (en) 2015-09-29
ES2765206T3 (es) 2020-06-08

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