RU2651483C2 - Способы очистки антител - Google Patents

Способы очистки антител Download PDF

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Publication number
RU2651483C2
RU2651483C2 RU2015136860A RU2015136860A RU2651483C2 RU 2651483 C2 RU2651483 C2 RU 2651483C2 RU 2015136860 A RU2015136860 A RU 2015136860A RU 2015136860 A RU2015136860 A RU 2015136860A RU 2651483 C2 RU2651483 C2 RU 2651483C2
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RU
Russia
Prior art keywords
protein
acid
sodium
buffer
contaminant
Prior art date
Application number
RU2015136860A
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English (en)
Russian (ru)
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RU2015136860A (ru
Inventor
Кент И. ГОКЛЕН
Эрик Дж. Суда
Антонио Рауль УБЬЕРА
Original Assignee
ГлаксоСмитКлайн Интеллекчуал Проперти (N2) Лимитед
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50391244&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=RU2651483(C2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ГлаксоСмитКлайн Интеллекчуал Проперти (N2) Лимитед filed Critical ГлаксоСмитКлайн Интеллекчуал Проперти (N2) Лимитед
Publication of RU2015136860A publication Critical patent/RU2015136860A/ru
Application granted granted Critical
Publication of RU2651483C2 publication Critical patent/RU2651483C2/ru

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
RU2015136860A 2013-03-15 2014-03-13 Способы очистки антител RU2651483C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361787309P 2013-03-15 2013-03-15
US61/787,309 2013-03-15
PCT/IB2014/059755 WO2014141150A1 (en) 2013-03-15 2014-03-13 Methods for purifying antibodies

Publications (2)

Publication Number Publication Date
RU2015136860A RU2015136860A (ru) 2017-04-28
RU2651483C2 true RU2651483C2 (ru) 2018-04-19

Family

ID=50391244

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2015136860A RU2651483C2 (ru) 2013-03-15 2014-03-13 Способы очистки антител

Country Status (15)

Country Link
US (3) US10676503B2 (https=)
EP (3) EP2969098B1 (https=)
JP (2) JP6599773B2 (https=)
KR (1) KR102114021B1 (https=)
CN (1) CN105188872B (https=)
AU (1) AU2014229280B2 (https=)
BR (1) BR112015022321A2 (https=)
CA (1) CA2902287A1 (https=)
DK (2) DK3680000T3 (https=)
ES (2) ES2765206T3 (https=)
IL (1) IL240753B (https=)
RU (1) RU2651483C2 (https=)
SG (1) SG11201506494XA (https=)
TW (1) TWI625335B (https=)
WO (1) WO2014141150A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2969098B1 (en) 2013-03-15 2019-10-30 Glaxosmithkline Intellectual Property (No. 2) Limited Methods for purifying antibodies
EP2997036B1 (en) 2013-05-15 2021-03-03 Medimmune Limited Purification of recombinantly produced polypeptides
US20170044210A1 (en) 2014-04-30 2017-02-16 Novo Nordisk A/S Methods for the purification of proteins using caprylic acid
CN108064286A (zh) 2015-01-26 2018-05-22 株式会社钟化 突变型免疫球蛋白κ链可变区结合性肽
WO2016121701A1 (ja) 2015-01-26 2016-08-04 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
WO2017195638A1 (ja) * 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
RU2759909C2 (ru) * 2016-09-07 2021-11-18 Глэксосмитклайн Интеллекчуал Проперти Дивелопмент Лимитед Способы очистки антител
CN106939045B (zh) * 2017-05-01 2020-03-31 深圳市国创纳米抗体技术有限公司 一种单克隆抗体细胞培养液澄清的方法
CN108085358A (zh) * 2018-02-08 2018-05-29 保定冀中药业有限公司 一种犬细小病毒单克隆抗体IgG2b-Fab片段的制备方法
TW202003555A (zh) * 2018-03-07 2020-01-16 英商葛蘭素史克智慧財產發展有限公司 用於純化重組多肽之方法
TW202000891A (zh) 2018-03-07 2020-01-01 英商葛蘭素史克智慧財產發展有限公司 純化抗體之方法
US20230302381A1 (en) * 2020-08-18 2023-09-28 Sartorius Bia Separations D.O.O. Metal-affinity extraction of host cell dna
CN114133446A (zh) * 2021-12-31 2022-03-04 方坦思(上海)生物医药有限公司 单克隆抗体的纯化方法
CN116179582A (zh) * 2022-09-27 2023-05-30 南京医事达生物科技有限公司 一种通用型基因重组蛋白质a亲和纯化填料制备方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012135415A1 (en) * 2011-03-29 2012-10-04 Glaxosmithkline Llc Buffer system for protein purification

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JPS57106673A (en) 1980-12-24 1982-07-02 Chugai Pharmaceut Co Ltd Dibenzo(b,f)(1,4)oxazepin derivative
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US6955917B2 (en) 1997-06-20 2005-10-18 Bayer Healthcare Llc Chromatographic method for high yield purification and viral inactivation of antibodies
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
DE60203176T2 (de) * 2001-06-01 2006-04-27 Upfront Chromatography A/S, Kopenhagen Fraktionierung von proteinhaltigen mischungen
PT1419179E (pt) 2001-08-10 2010-03-16 Univ Aberdeen Domínios de ligação de antigenes
MXPA04011769A (es) 2002-05-31 2005-07-26 Indian Agricultural Council Deteccion rapida de toxinas cry de bacillus thuringiensis.
CA2557229C (en) 2004-02-27 2012-07-10 Octapharma Ag A method of providing a purified, virus safe antibody preparation
WO2005113604A2 (en) * 2004-05-14 2005-12-01 Hematech, Llc Methods for immunoglobulin purification
WO2007109163A2 (en) * 2006-03-16 2007-09-27 Amgen Inc Wash buffer and method of using
CN101511387A (zh) 2006-09-08 2009-08-19 惠氏公司 使用亲和色谱法纯化蛋白质的精氨酸洗涤液
WO2008085988A1 (en) * 2007-01-05 2008-07-17 Amgen Inc. Methods of purifying proteins
EP2969098B1 (en) * 2013-03-15 2019-10-30 Glaxosmithkline Intellectual Property (No. 2) Limited Methods for purifying antibodies
EP2997036B1 (en) * 2013-05-15 2021-03-03 Medimmune Limited Purification of recombinantly produced polypeptides
CN120904322A (zh) 2013-09-13 2025-11-07 豪夫迈·罗氏有限公司 包含纯化的重组多肽的方法和组合物

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012135415A1 (en) * 2011-03-29 2012-10-04 Glaxosmithkline Llc Buffer system for protein purification

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
WO 2012135415 A1, 04.10.2012, формула. Brodsky Y. et al. Caprylic acid precipitation method for impurity reduction: an alternative to conventional chromatography for monoclonal antibody purification //Biotechnology and bioengineering. - 2012. - Vol. 109. - n. 10. - P. 2589-2598. *
формула. Brodsky Y. et al. Caprylic acid precipitation method for impurity reduction: an alternative to conventional chromatography for monoclonal antibody purification //Biotechnology and bioengineering. - 2012. - Vol. 109. - n. 10. - P. 2589-2598. *

Also Published As

Publication number Publication date
JP2016519052A (ja) 2016-06-30
KR102114021B1 (ko) 2020-05-22
US10676503B2 (en) 2020-06-09
CN105188872B (zh) 2019-01-15
EP2969098B1 (en) 2019-10-30
JP6599773B2 (ja) 2019-10-30
WO2014141150A1 (en) 2014-09-18
BR112015022321A2 (pt) 2017-07-18
CN105188872A (zh) 2015-12-23
EP4371996A2 (en) 2024-05-22
EP4371996A3 (en) 2024-08-14
US20200277330A1 (en) 2020-09-03
IL240753B (en) 2021-01-31
US12358945B2 (en) 2025-07-15
DK2969098T3 (da) 2020-01-27
US20250353878A1 (en) 2025-11-20
CA2902287A1 (en) 2014-09-18
US20160024146A1 (en) 2016-01-28
IL240753A0 (en) 2015-10-29
DK3680000T3 (da) 2024-04-02
EP3680000A1 (en) 2020-07-15
RU2015136860A (ru) 2017-04-28
EP3680000B1 (en) 2024-03-13
TW201522365A (zh) 2015-06-16
JP2020019795A (ja) 2020-02-06
AU2014229280A1 (en) 2015-10-01
KR20150131235A (ko) 2015-11-24
AU2014229280B2 (en) 2017-01-05
TWI625335B (zh) 2018-06-01
ES2978309T3 (es) 2024-09-10
JP7002511B2 (ja) 2022-02-21
EP2969098A1 (en) 2016-01-20
SG11201506494XA (en) 2015-09-29
ES2765206T3 (es) 2020-06-08

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