BR112013000623A2 - purificação de proteína. - Google Patents

purificação de proteína.

Info

Publication number
BR112013000623A2
BR112013000623A2 BR112013000623A BR112013000623A BR112013000623A2 BR 112013000623 A2 BR112013000623 A2 BR 112013000623A2 BR 112013000623 A BR112013000623 A BR 112013000623A BR 112013000623 A BR112013000623 A BR 112013000623A BR 112013000623 A2 BR112013000623 A2 BR 112013000623A2
Authority
BR
Brazil
Prior art keywords
protein purification
exchange chromatography
chromatography step
purifying
antibody fragment
Prior art date
Application number
BR112013000623A
Other languages
English (en)
Other versions
BR112013000623B1 (pt
Inventor
Jonathan Symmons
Mark Robert Pearce-Higgins
Mariangela Spitali
Richard Whitcombe
Original Assignee
Ucb Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb Pharma Sa filed Critical Ucb Pharma Sa
Publication of BR112013000623A2 publication Critical patent/BR112013000623A2/pt
Publication of BR112013000623B1 publication Critical patent/BR112013000623B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)

Abstract

purificação de proteína. a presente invenção diz respeito a um processo para a purificação de um fragmento de anticorpo a partir de um extrato de célula periplásmica que compreende uma primeira etapa de cromatografia de troca de cátion e uma segunda etapa de cromatografia de troca aniônica.
BR112013000623-4A 2010-07-27 2011-07-26 Processo para a purificação de um fragmento de anticorpo a partir de extrato de célula periplásmica de bactérias gram-negativas BR112013000623B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1012603.5A GB201012603D0 (en) 2010-07-27 2010-07-27 Protein purification
GB1012603.5 2010-07-27
PCT/EP2011/062837 WO2012013682A2 (en) 2010-07-27 2011-07-26 Protein purification

Publications (2)

Publication Number Publication Date
BR112013000623A2 true BR112013000623A2 (pt) 2016-05-24
BR112013000623B1 BR112013000623B1 (pt) 2022-10-11

Family

ID=42752872

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013000623-4A BR112013000623B1 (pt) 2010-07-27 2011-07-26 Processo para a purificação de um fragmento de anticorpo a partir de extrato de célula periplásmica de bactérias gram-negativas

Country Status (22)

Country Link
US (4) US9309280B2 (pt)
EP (2) EP3747893A1 (pt)
JP (1) JP5893622B2 (pt)
KR (1) KR101921767B1 (pt)
CN (2) CN107793477A (pt)
AU (1) AU2011284896B2 (pt)
BR (1) BR112013000623B1 (pt)
CA (1) CA2805132C (pt)
CY (1) CY1123619T1 (pt)
DK (1) DK2598514T3 (pt)
ES (1) ES2834967T3 (pt)
GB (1) GB201012603D0 (pt)
HK (1) HK1246330A1 (pt)
HU (1) HUE052159T2 (pt)
IL (1) IL223931A (pt)
LT (1) LT2598514T (pt)
PL (1) PL2598514T3 (pt)
PT (1) PT2598514T (pt)
RS (1) RS61095B1 (pt)
SG (2) SG10201802316VA (pt)
SI (1) SI2598514T1 (pt)
WO (1) WO2012013682A2 (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201012603D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Protein purification
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
SG11201408502XA (en) * 2012-06-21 2015-01-29 Synthon Biopharmaceuticals Bv Method of purifying an antibody
CN104903442A (zh) * 2013-01-09 2015-09-09 夏尔人类遗传性治疗公司 含用于纯化芳基硫酸酯酶a的方法
WO2014178078A2 (en) 2013-04-30 2014-11-06 Intas Boipharmaceuticals Limited Novel cloning, expression & purification method for the preparation of ranibizumab
WO2015088677A1 (en) 2013-12-12 2015-06-18 Emd Millipore Corporation Protein separations using an acrylamide containing filter
KR102537099B1 (ko) 2014-12-22 2023-05-25 유씨비 바이오파마 에스알엘 단백질 제조 방법
CA2976005A1 (en) 2015-02-06 2016-08-11 Cell Idx, Inc. Antigen-coupled immunoreagents
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
GB201602938D0 (en) * 2016-02-19 2016-04-06 Ucb Biopharma Sprl Protein purification
CN109716130B (zh) * 2016-07-18 2023-05-23 赛尔伊迪克斯公司 抗原偶联的杂交试剂
BR112019022356A2 (pt) 2017-04-27 2020-05-26 Juno Therapeutics Gmbh Reagentes de partícula oligoméricos e métodos de uso dos mesmos
US10717023B1 (en) * 2017-10-11 2020-07-21 Roddy Kevin Stafford Method for continuous purification
CN110241012B (zh) * 2018-03-09 2022-11-01 嘉和生物药业有限公司 一种生物大分子上游分阶段截留的生产方法、生产模块及在生产中的应用
WO2021176311A1 (en) * 2020-03-05 2021-09-10 Lupin Limited An improved purification process for ranibizumab
US20230129803A1 (en) * 2021-10-27 2023-04-27 Plasma Technologies, Llc Compositions and methods for isolating proteins
US11926669B2 (en) 2022-05-30 2024-03-12 Hanall Biopharma Co., Ltd. Anti-FcRn antibody or antigen binding fragment thereof with improved stability

Family Cites Families (19)

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Publication number Priority date Publication date Assignee Title
US5118796A (en) * 1987-12-09 1992-06-02 Centocor, Incorporated Efficient large-scale purification of immunoglobulins and derivatives
US5655866A (en) 1995-03-20 1997-08-12 Bellanca; Joseph V. Method of folding a signature for use in bookbinding
DK1864999T3 (da) 1996-11-27 2009-06-29 Genentech Inc Affinitetsoprensning af polypeptid på protein A-matrix
WO1999033976A1 (en) * 1997-12-24 1999-07-08 Takeda Chemical Industries, Ltd. Polypeptide, their production and use
US6489447B1 (en) 1998-05-06 2002-12-03 Genentech, Inc. Protein purification
GB0013810D0 (en) * 2000-06-06 2000-07-26 Celltech Chiroscience Ltd Biological products
EP1501369B1 (en) * 2002-04-26 2015-06-24 Genentech, Inc. Non-affinity purification of proteins
CA2496060C (en) 2002-09-11 2015-08-04 Genentech, Inc. Protein purification by ion exchange chromatography
GB0224082D0 (en) * 2002-10-16 2002-11-27 Celltech R&D Ltd Biological products
AT412785B (de) * 2003-12-04 2005-07-25 Kungl Andreas J Dr Gag-bindungsproteine
PL1869065T3 (pl) * 2005-03-11 2020-09-21 Wyeth Llc Sposób prowadzenia chromatografii podziałowej ze słabym wiązaniem
KR20080111487A (ko) * 2006-03-20 2008-12-23 메다렉스, 인코포레이티드 단백질 정제 방법
NZ610566A (en) 2006-04-05 2014-09-26 Abbvie Biotechnology Ltd Antibody purification
US20080000431A1 (en) 2006-06-29 2008-01-03 Stephen Longo Dog leash assembly
ES2572958T3 (es) 2007-10-30 2016-06-03 Genentech, Inc. Purificación de anticuerpos mediante cromatografía de intercambio catiónico
WO2009135656A1 (en) * 2008-05-06 2009-11-12 Lonza Biologics Plc. A method for the purification of antibodies using displacement chromatography
JP2012506384A (ja) * 2008-10-20 2012-03-15 アボット・ラボラトリーズ Ｉｌ−１２に結合する抗体およびそれを精製する方法
CN104974251A (zh) * 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
GB201012603D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Protein purification

Also Published As

Publication number Publication date
IL223931A (en) 2017-10-31
CN103025752A (zh) 2013-04-03
US20190135909A1 (en) 2019-05-09
US20160108119A1 (en) 2016-04-21
EP2598514B1 (en) 2020-09-09
KR101921767B1 (ko) 2018-11-23
JP2013532673A (ja) 2013-08-19
JP5893622B2 (ja) 2016-03-23
HK1246330A1 (zh) 2018-09-07
KR20130131307A (ko) 2013-12-03
SG10201802316VA (en) 2018-04-27
CA2805132C (en) 2020-01-14
ES2834967T3 (es) 2021-06-21
AU2011284896A1 (en) 2013-01-24
EP2598514A2 (en) 2013-06-05
PT2598514T (pt) 2020-12-15
US10189897B2 (en) 2019-01-29
CN107793477A (zh) 2018-03-13
SG186957A1 (en) 2013-02-28
AU2011284896B2 (en) 2016-06-16
SI2598514T1 (sl) 2021-01-29
CY1123619T1 (el) 2022-03-24
RS61095B1 (sr) 2020-12-31
LT2598514T (lt) 2020-12-28
WO2012013682A2 (en) 2012-02-02
US20220289832A1 (en) 2022-09-15
BR112013000623B1 (pt) 2022-10-11
WO2012013682A3 (en) 2012-05-18
US9309280B2 (en) 2016-04-12
HUE052159T2 (hu) 2021-04-28
PL2598514T3 (pl) 2021-03-08
DK2598514T3 (da) 2020-11-16
EP3747893A1 (en) 2020-12-09
US11339214B2 (en) 2022-05-24
CA2805132A1 (en) 2012-02-02
US20130184439A1 (en) 2013-07-18
GB201012603D0 (en) 2010-09-08

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NAO 10196/2001, QUE MODIFICOU A LEI NAO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUAANCIA PRA VIA DA ANVISA. CONSIDERANDO A APROVAA AO DOS TERMOS DO PARECER NAO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NAO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDAANCIAS CABA-VEIS.

B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]

Free format text: NOTIFICACAO DE DEVOLUCAO DO PEDIDO EM FUNCAO DA REVOGACAO DO ART. 229-C DA LEI NO 9.279, DE 1996, POR FORCA DA LEI NO 14.195, DE 2021

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 26/07/2011, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO.