CY1121394T1 - Μεθοδος καθαρισμου αντισωματος - Google Patents

Μεθοδος καθαρισμου αντισωματος

Info

Publication number
CY1121394T1
CY1121394T1 CY20181101381T CY181101381T CY1121394T1 CY 1121394 T1 CY1121394 T1 CY 1121394T1 CY 20181101381 T CY20181101381 T CY 20181101381T CY 181101381 T CY181101381 T CY 181101381T CY 1121394 T1 CY1121394 T1 CY 1121394T1
Authority
CY
Cyprus
Prior art keywords
cleaning method
antibody
anion exchange
exchange chromatography
antibody composition
Prior art date
Application number
CY20181101381T
Other languages
English (en)
Inventor
Bastiaan Pieter Arian KOKKE
Everdina Josephina Wilhelmina VAN WIJK-BASTEN
Thomas Antonius Bernardus DE BEIJER
Michel Hendrikus Maria Eppink
Maria MARZÁ PÉREZ
Original Assignee
Synthon Biopharmaceuticals B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthon Biopharmaceuticals B.V. filed Critical Synthon Biopharmaceuticals B.V.
Publication of CY1121394T1 publication Critical patent/CY1121394T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Oncology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)

Abstract

Μία μέθοδος καθαρισμού σύνθεσης αντισώματος περιλαμβάνει εφαρμογή χρωματογραφίας ανταλλαγής ανιόντων όψιμα στην διαδικασία καθαρισμού. Μία καθαρισμένη με υπερδιήθηση/διαδιήθηση σύνθεση αντισώματος υποβάλλεται σε χρωματογραφία ανταλλαγής ανιόντων (AEX) για να σχηματισθεί μία φαρμακευτικώς καθαρή σύνθεση αντισώματος.
CY20181101381T 2012-06-21 2018-12-20 Μεθοδος καθαρισμου αντισωματος CY1121394T1 (el)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2012/062014 WO2013189544A1 (en) 2012-06-21 2012-06-21 Method of purifying an antibody

Publications (1)

Publication Number Publication Date
CY1121394T1 true CY1121394T1 (el) 2020-05-29

Family

ID=46321045

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20181101381T CY1121394T1 (el) 2012-06-21 2018-12-20 Μεθοδος καθαρισμου αντισωματος

Country Status (14)

Country Link
US (1) US9845338B2 (el)
EP (1) EP2864346B1 (el)
KR (1) KR101941742B1 (el)
CN (1) CN104487448B (el)
CY (1) CY1121394T1 (el)
DK (1) DK2864346T3 (el)
ES (1) ES2704487T3 (el)
HR (1) HRP20190030T1 (el)
HU (1) HUE043121T2 (el)
LT (1) LT2864346T (el)
PL (1) PL2864346T3 (el)
PT (1) PT2864346T (el)
SG (1) SG11201408502XA (el)
WO (1) WO2013189544A1 (el)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7032046B2 (ja) * 2016-01-22 2022-03-08 旭化成メディカル株式会社 生理活性物質の連続的な定流速精製方法
WO2017143286A1 (en) * 2016-02-19 2017-08-24 Wilfred Chen Functionalized nanoparticles for enhanced affinity precipitation of proteins
EP3769083A1 (en) 2018-03-21 2021-01-27 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
KR102337683B1 (ko) * 2018-09-21 2021-12-13 주식회사 녹십자 고효율 항-tfpi 항체 조성물
WO2020247574A1 (en) * 2019-06-05 2020-12-10 Seattle Genetics, Inc. Methods of purifying masked antibodies
CN114539351B (zh) * 2022-01-26 2023-03-03 上海健士拜生物科技有限公司 纳米抗体的纯化方法
CN114409787A (zh) * 2022-02-18 2022-04-29 南京英瀚斯生物科技有限公司 一种针对her3纳米抗体的分离纯化方法
WO2023249892A1 (en) * 2022-06-20 2023-12-28 Amgen Inc. Clearance of aggregates from uf/df pools in downstream antibody purification

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7452539B2 (en) * 2001-12-19 2008-11-18 Genentech, Inc. Stabilizing polypeptides which have been exposed to urea
PT1718675E (pt) * 2004-02-27 2013-05-21 Octapharma Ag Método de proporcionar uma preparação purificada de anticorpos livre de vírus
EP1967529A1 (en) * 2004-07-20 2008-09-10 Symphogen A/S Anti-rhesus D recombinant polyclonal antibody and methods of manufacture
WO2007117490A2 (en) * 2006-04-05 2007-10-18 Abbott Biotechnology Ltd. Antibody purification
CN101945890A (zh) * 2007-12-21 2011-01-12 健泰科生物技术公司 抗cd20抗体的结晶
SG195574A1 (en) * 2008-10-20 2013-12-30 Abbott Lab Antibodies that bind to il-18 and methods of purifying the same
SG175142A1 (en) * 2009-04-27 2011-12-29 Immuron Ltd Anti-lps enriched immunoglobulin preparation for use in treatment and/or prophylaxis of a pathologic disorder
DK2443149T3 (en) * 2009-06-15 2016-12-12 Vib Vzw BACE1 inhibitory ANTIBODIES
US9345661B2 (en) * 2009-07-31 2016-05-24 Genentech, Inc. Subcutaneous anti-HER2 antibody formulations and uses thereof
WO2011015920A2 (en) * 2009-08-03 2011-02-10 Avesthagen Limited A highly efficient process of purification and production of recombinant trastuzumab
GB201012603D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Protein purification
WO2012125735A1 (en) * 2011-03-15 2012-09-20 Abott Laboratories An integrated approach to the isolation and purification of antibodies

Also Published As

Publication number Publication date
LT2864346T (lt) 2019-01-25
PT2864346T (pt) 2018-12-12
CN104487448A (zh) 2015-04-01
PL2864346T3 (pl) 2019-04-30
CN104487448B (zh) 2020-04-24
DK2864346T3 (en) 2019-02-04
EP2864346A1 (en) 2015-04-29
HRP20190030T1 (hr) 2019-02-22
EP2864346B1 (en) 2018-10-17
US20160002289A1 (en) 2016-01-07
WO2013189544A1 (en) 2013-12-27
HUE043121T2 (hu) 2019-08-28
KR20150027807A (ko) 2015-03-12
US9845338B2 (en) 2017-12-19
KR101941742B1 (ko) 2019-01-23
ES2704487T3 (es) 2019-03-18
SG11201408502XA (en) 2015-01-29

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