BR112015016005B1 - Método de preparar uma biblioteca imobilizada de fragmentos de dna marcados e suporte sólido contendo a dita biblioteca - Google Patents

Método de preparar uma biblioteca imobilizada de fragmentos de dna marcados e suporte sólido contendo a dita biblioteca Download PDF

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BR112015016005B1
BR112015016005B1 BR112015016005-0A BR112015016005A BR112015016005B1 BR 112015016005 B1 BR112015016005 B1 BR 112015016005B1 BR 112015016005 A BR112015016005 A BR 112015016005A BR 112015016005 B1 BR112015016005 B1 BR 112015016005B1
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immobilized
polynucleotide
solid support
dna
transposome
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BR112015016005-0A
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Portuguese (pt)
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BR112015016005A2 (pt
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Niall Anthony Gormley
Geoffrey Paul Smith
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Illumina Cambridge Limited
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/543—Immobilised enzyme(s)
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target
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    • C40—COMBINATORIAL TECHNOLOGY
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    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
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    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support

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  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
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  • Structural Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)
BR112015016005-0A 2013-01-09 2014-01-08 Método de preparar uma biblioteca imobilizada de fragmentos de dna marcados e suporte sólido contendo a dita biblioteca BR112015016005B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361750682P 2013-01-09 2013-01-09
US61/750,682 2013-01-09
PCT/IB2014/000610 WO2014108810A2 (en) 2013-01-09 2014-01-08 Sample preparation on a solid support

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BR112015016005A2 BR112015016005A2 (pt) 2018-04-24
BR112015016005B1 true BR112015016005B1 (pt) 2021-11-16

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KR (4) KR102602143B1 (enExample)
CN (2) CN104968805B (enExample)
BR (1) BR112015016005B1 (enExample)
CA (1) CA2895260A1 (enExample)
CY (1) CY1121101T1 (enExample)
DK (1) DK2943589T3 (enExample)
ES (2) ES2979286T3 (enExample)
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RS (1) RS57954B1 (enExample)
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