SG11202102424QA - Methods and means for preparing a library for sequencing - Google Patents
Methods and means for preparing a library for sequencingInfo
- Publication number
- SG11202102424QA SG11202102424QA SG11202102424QA SG11202102424QA SG11202102424QA SG 11202102424Q A SG11202102424Q A SG 11202102424QA SG 11202102424Q A SG11202102424Q A SG 11202102424QA SG 11202102424Q A SG11202102424Q A SG 11202102424QA SG 11202102424Q A SG11202102424Q A SG 11202102424QA
- Authority
- SG
- Singapore
- Prior art keywords
- sequencing
- library
- preparing
- methods
- Prior art date
Links
- 238000000034 method Methods 0.000 title 1
- 238000012163 sequencing technique Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862780812P | 2018-12-17 | 2018-12-17 | |
| PCT/US2019/066272 WO2020131626A1 (en) | 2018-12-17 | 2019-12-13 | Methods and means for preparing a library for sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202102424QA true SG11202102424QA (en) | 2021-04-29 |
Family
ID=69160410
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202102424QA SG11202102424QA (en) | 2018-12-17 | 2019-12-13 | Methods and means for preparing a library for sequencing |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20220195507A1 (en) |
| EP (1) | EP3899028A1 (en) |
| JP (1) | JP2022515002A (en) |
| KR (1) | KR20210104650A (en) |
| CN (1) | CN112867800A (en) |
| AU (1) | AU2019402067A1 (en) |
| BR (1) | BR112021006095A2 (en) |
| CA (1) | CA3115155A1 (en) |
| IL (1) | IL281552A (en) |
| MX (1) | MX2021003769A (en) |
| SG (1) | SG11202102424QA (en) |
| WO (1) | WO2020131626A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3788171B1 (en) | 2018-05-03 | 2023-04-05 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
| CN112410403B (en) * | 2020-11-04 | 2022-04-22 | 苏州京脉生物科技有限公司 | Preparation method and kit of ATAC-seq library with accurate quantification |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| US5965443A (en) | 1996-09-09 | 1999-10-12 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| US5925545A (en) | 1996-09-09 | 1999-07-20 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| WO2001009363A1 (en) | 1999-08-02 | 2001-02-08 | Wisconsin Alumni Research Foundation | Mutant tn5 transposase enzymes and method for their use |
| US7083980B2 (en) | 2003-04-17 | 2006-08-01 | Wisconsin Alumni Research Foundation | Tn5 transposase mutants and the use thereof |
| EP3175914A1 (en) | 2004-01-07 | 2017-06-07 | Illumina Cambridge Limited | Improvements in or relating to molecular arrays |
| US7608434B2 (en) | 2004-08-04 | 2009-10-27 | Wisconsin Alumni Research Foundation | Mutated Tn5 transposase proteins and the use thereof |
| JP5794572B2 (en) | 2008-10-24 | 2015-10-14 | エピセンター テクノロジーズ コーポレイションＥｐｉｃｅｎｔｒｅ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｃｏｒｐｏｒａｔｉｏｎ | Transposon end compositions and methods for modifying nucleic acids |
| US9005935B2 (en) | 2011-05-23 | 2015-04-14 | Agilent Technologies, Inc. | Methods and compositions for DNA fragmentation and tagging by transposases |
| US20130143774A1 (en) | 2011-12-05 | 2013-06-06 | The Regents Of The University Of California | Methods and compositions for generating polynucleic acid fragments |
| US9012022B2 (en) * | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
| US9644199B2 (en) * | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
| EP4321628A3 (en) * | 2013-05-23 | 2024-04-24 | The Board of Trustees of the Leland Stanford Junior University | Transposition into native chromatin for personal epigenomics |
| EP3083994B1 (en) * | 2013-12-20 | 2021-08-18 | Illumina, Inc. | Preserving genomic connectivity information in fragmented genomic dna samples |
| AU2015247779B2 (en) | 2014-04-15 | 2021-06-24 | Illumina, Inc. | Modified transposases for improved insertion sequence bias and increased DNA input tolerance |
| CA2947426C (en) * | 2014-04-29 | 2020-01-07 | Illumina, Inc. | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| WO2015189636A1 (en) * | 2014-06-13 | 2015-12-17 | Illumina Cambridge Limited | Methods and compositions for preparing sequencing libraries |
| SG10201912283RA (en) * | 2015-08-28 | 2020-02-27 | Illumina Inc | Nucleic acid sequence analysis from single cells |
| CN107794258A (en) * | 2016-08-31 | 2018-03-13 | 安诺优达基因科技(北京)有限公司 | A kind of method and its application in constructed dna large fragment library |
| WO2018218226A1 (en) * | 2017-05-26 | 2018-11-29 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN108866155B (en) * | 2018-06-11 | 2022-07-26 | 中国农业科学院深圳农业基因组研究所 | Preparation method of next generation sequencing library |
-
2019
- 2019-12-13 JP JP2021514960A patent/JP2022515002A/en active Pending
- 2019-12-13 KR KR1020217009645A patent/KR20210104650A/en active Search and Examination
- 2019-12-13 SG SG11202102424QA patent/SG11202102424QA/en unknown
- 2019-12-13 MX MX2021003769A patent/MX2021003769A/en unknown
- 2019-12-13 WO PCT/US2019/066272 patent/WO2020131626A1/en unknown
- 2019-12-13 AU AU2019402067A patent/AU2019402067A1/en active Pending
- 2019-12-13 CN CN201980064423.0A patent/CN112867800A/en active Pending
- 2019-12-13 EP EP19836392.1A patent/EP3899028A1/en active Pending
- 2019-12-13 US US17/250,846 patent/US20220195507A1/en active Pending
- 2019-12-13 CA CA3115155A patent/CA3115155A1/en active Pending
- 2019-12-13 BR BR112021006095-2A patent/BR112021006095A2/en unknown
-
2021
- 2021-03-16 IL IL281552A patent/IL281552A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3899028A1 (en) | 2021-10-27 |
| JP2022515002A (en) | 2022-02-17 |
| KR20210104650A (en) | 2021-08-25 |
| IL281552A (en) | 2021-05-31 |
| CA3115155A1 (en) | 2020-06-25 |
| MX2021003769A (en) | 2021-05-27 |
| WO2020131626A1 (en) | 2020-06-25 |
| BR112021006095A2 (en) | 2021-07-20 |
| AU2019402067A1 (en) | 2021-05-06 |
| US20220195507A1 (en) | 2022-06-23 |
| CN112867800A (en) | 2021-05-28 |
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