BR112013002981A2 - processo de transfecção de plantas. - Google Patents

processo de transfecção de plantas.

Info

Publication number
BR112013002981A2
BR112013002981A2 BR112013002981A BR112013002981A BR112013002981A2 BR 112013002981 A2 BR112013002981 A2 BR 112013002981A2 BR 112013002981 A BR112013002981 A BR 112013002981A BR 112013002981 A BR112013002981 A BR 112013002981A BR 112013002981 A2 BR112013002981 A2 BR 112013002981A2
Authority
BR
Brazil
Prior art keywords
plant
transfection process
agrobacterium strain
plant transfection
dna
Prior art date
Application number
BR112013002981A
Other languages
English (en)
Other versions
BR112013002981B1 (pt
Inventor
Anatoli Giritch
Anton Shvarts
Doreen Tiede
Patrick Roemer
Simone Hahn
Yuri Gleba
Yuri Symonenko
Original Assignee
Nomad Bioscience Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nomad Bioscience Gmbh filed Critical Nomad Bioscience Gmbh
Publication of BR112013002981A2 publication Critical patent/BR112013002981A2/pt
Publication of BR112013002981B1 publication Critical patent/BR112013002981B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance

Abstract

processo de transfecção de plantas. um processo de transfecção de uma planta compreendendo pulverização de partes da referida planta com uma suspensão aquosa contendo células de uma cepa de agrobacterium e pelo menos um abrasivo suspenso na referida suspensão, a referida cepa de agrobacterium compreendendo uma molécula de dna que compreende um construto de ácido nucleico contendo uma sequência de dna de interesse a ser transfectada na planta.
BR112013002981-1A 2010-08-07 2011-05-06 Processos de geração ou alteração de um traço de uma planta, de produção de uma proteína de interesse em uma planta e de proteção de plantas de cultura em um campo contra uma praga BR112013002981B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP10008267.6 2010-08-07
EP10008267 2010-08-07
EP10008393.0 2010-08-11
EP10008393A EP2418283A1 (en) 2010-08-07 2010-08-11 Process of transfecting plants
PCT/EP2011/002279 WO2012019660A1 (en) 2010-08-07 2011-05-06 Process of transfecting plants

Publications (2)

Publication Number Publication Date
BR112013002981A2 true BR112013002981A2 (pt) 2016-06-07
BR112013002981B1 BR112013002981B1 (pt) 2019-07-09

Family

ID=42941837

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013002981-1A BR112013002981B1 (pt) 2010-08-07 2011-05-06 Processos de geração ou alteração de um traço de uma planta, de produção de uma proteína de interesse em uma planta e de proteção de plantas de cultura em um campo contra uma praga

Country Status (11)

Country Link
US (2) US20130212739A1 (pt)
EP (2) EP2418283A1 (pt)
JP (1) JP5898681B2 (pt)
CN (1) CN103282499A (pt)
AU (1) AU2011288653B2 (pt)
BR (1) BR112013002981B1 (pt)
CA (1) CA2807544C (pt)
ES (1) ES2654583T3 (pt)
MX (1) MX346926B (pt)
RU (1) RU2013110019A (pt)
WO (1) WO2012019660A1 (pt)

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Also Published As

Publication number Publication date
US20130212739A1 (en) 2013-08-15
CA2807544C (en) 2020-03-24
CA2807544A1 (en) 2012-02-16
EP2601295A1 (en) 2013-06-12
EP2601295B1 (en) 2017-12-13
RU2013110019A (ru) 2014-09-20
WO2012019660A1 (en) 2012-02-16
JP2013532992A (ja) 2013-08-22
AU2011288653A1 (en) 2013-02-28
ES2654583T3 (es) 2018-02-14
BR112013002981B1 (pt) 2019-07-09
MX346926B (es) 2017-04-05
MX2013001459A (es) 2013-07-05
CN103282499A (zh) 2013-09-04
AU2011288653B2 (en) 2014-10-09
JP5898681B2 (ja) 2016-04-06
EP2418283A1 (en) 2012-02-15
US20190136260A1 (en) 2019-05-09

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