BR112012017124C1 - Método para produzir e para purificar um multímero polipeptídico - Google Patents

Método para produzir e para purificar um multímero polipeptídico

Info

Publication number
BR112012017124C1
BR112012017124C1 BR112012017124A BR112012017124A BR112012017124C1 BR 112012017124 C1 BR112012017124 C1 BR 112012017124C1 BR 112012017124 A BR112012017124 A BR 112012017124A BR 112012017124 A BR112012017124 A BR 112012017124A BR 112012017124 C1 BR112012017124 C1 BR 112012017124C1
Authority
BR
Brazil
Prior art keywords
purifying
present
multispecific antibodies
protein
polypeptide
Prior art date
Application number
BR112012017124A
Other languages
English (en)
Other versions
BR112012017124B8 (pt
BR112012017124B1 (pt
BR112012017124A2 (pt
Inventor
Eriko Ito
Tetsuya Wakabayashi
Tomoyuki Igawa
Zenjiro Sampei
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of BR112012017124A2 publication Critical patent/BR112012017124A2/pt
Publication of BR112012017124B1 publication Critical patent/BR112012017124B1/pt
Publication of BR112012017124B8 publication Critical patent/BR112012017124B8/pt
Publication of BR112012017124C1 publication Critical patent/BR112012017124C1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70535—Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/303—Liver or Pancreas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567—Framework region [FR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/66—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a swap of domains, e.g. CH3-CH2, VH-CL or VL-CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Abstract

método para produção de um multímero polipeptídico, multíme-ros polipeptídicos, método para purificar um multímero polipep-tídico, ácido nucleico, vetor, célula, e composição farmacêutica. a presente invenção fornece métodos eficientes baseados na alteração da afinidade de ligação à proteína a, para produzir ou purificar anticorpos multiespecíficos que têm a atividade da ligação a dois ou mais tipos de antígenos com alta pureza por somente uma etapa de purificação baseada em proteína a. os métodos da presente invenção para produção ou purificação de anticorpos multiespecíficos que caracterizam resídu-os de aminoácido alterados da região constante e/ou região variável da cadeia pesada do anticorpo. os anticorpos multiespecíficos com uma atividade de ligação à proteína a alterada, que exibem retenção plasmática comparável ou mais longa do que aquela de igg1 humana, podem ser eficientemente preparados com alta pureza pela introdu-ção de alterações de aminoácidos da presente invenção em anticorpos.
BR112012017124A 2009-12-25 2010-12-24 Método para produzir e para purificar um multímero polipeptídico BR112012017124C1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009294391 2009-12-25
JP2009-294391 2009-12-25
PCT/JP2010/073361 WO2011078332A1 (ja) 2009-12-25 2010-12-24 ポリペプチド多量体を精製するためのポリペプチドの改変方法

Publications (4)

Publication Number Publication Date
BR112012017124A2 BR112012017124A2 (pt) 2017-10-31
BR112012017124B1 BR112012017124B1 (pt) 2021-04-06
BR112012017124B8 BR112012017124B8 (pt) 2021-05-25
BR112012017124C1 true BR112012017124C1 (pt) 2021-08-31

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012017124A BR112012017124C1 (pt) 2009-12-25 2010-12-24 Método para produzir e para purificar um multímero polipeptídico

Country Status (15)

Country Link
US (12) US20130018174A1 (pt)
EP (1) EP2522724B1 (pt)
JP (1) JP6087054B2 (pt)
KR (3) KR101856792B1 (pt)
CN (1) CN102770537A (pt)
AU (1) AU2010336277B2 (pt)
BR (1) BR112012017124C1 (pt)
CA (1) CA2785414C (pt)
ES (1) ES2777901T3 (pt)
HR (1) HRP20200464T1 (pt)
MX (1) MX2012007497A (pt)
PL (1) PL2522724T3 (pt)
RU (1) RU2606264C2 (pt)
SI (1) SI2522724T1 (pt)
WO (1) WO2011078332A1 (pt)

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US20180162902A1 (en) 2018-06-14
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US20190352334A1 (en) 2019-11-21
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