BG66119B1 - Методи за пречистване и регенерация на протеин - Google Patents

Методи за пречистване и регенерация на протеин

Info

Publication number
BG66119B1
BG66119B1 BG107844A BG10784403A BG66119B1 BG 66119 B1 BG66119 B1 BG 66119B1 BG 107844 A BG107844 A BG 107844A BG 10784403 A BG10784403 A BG 10784403A BG 66119 B1 BG66119 B1 BG 66119B1
Authority
BG
Bulgaria
Prior art keywords
beta
purification
ifn
recovery
protein
Prior art date
Application number
BG107844A
Other languages
English (en)
Other versions
BG107844A (bg
Inventor
Sid Wolfe
Irina Esikova
Susan Babuka
Bret Sherley
Dennis Fordham
Original Assignee
Chiron Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corporation filed Critical Chiron Corporation
Publication of BG107844A publication Critical patent/BG107844A/bg
Publication of BG66119B1 publication Critical patent/BG66119B1/bg

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)

Abstract

Изобретението се отнася до методи за пречистване и регенерация на интерферон-бета (IFN-бета) и до състави, включващи по същество мономерен IFN-бета. В един от методите по същество пречистен IFN-бета или негов вариант се утаява и след това се разтваря в разтвор на гуанидин хидрохлорид (HCl). Протеинът се ренатурира с разреждане с подходящ буфер. В друг метод отсъства етапът на утаяване. Следва направо ренатуриране на IFN-бета, остатъчният гуанидин НСl се отделя чрез диафилтруване или диализа с фармацевтично приемлив буфер, за да се получат фармацевтични състави, съдържащи по същество мономеренIFN-бета.
BG107844A 2000-10-27 2003-05-23 Методи за пречистване и регенерация на протеин BG66119B1 (bg)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US24396500P 2000-10-27 2000-10-27
US28260701P 2001-04-09 2001-04-09
US33037501P 2001-10-18 2001-10-18
US10/035,420 US7544354B2 (en) 2000-10-27 2001-10-25 Methods of protein purification and recovery
PCT/US2001/051038 WO2002034791A2 (en) 2000-10-27 2001-10-26 Methods of purification and recovery of interferon-beta

Publications (2)

Publication Number Publication Date
BG107844A BG107844A (bg) 2004-07-30
BG66119B1 true BG66119B1 (bg) 2011-05-31

Family

ID=27488301

Family Applications (1)

Application Number Title Priority Date Filing Date
BG107844A BG66119B1 (bg) 2000-10-27 2003-05-23 Методи за пречистване и регенерация на протеин

Country Status (16)

Country Link
US (3) US7544354B2 (bg)
EP (1) EP1366060B1 (bg)
JP (1) JP3946139B2 (bg)
CN (1) CN100493601C (bg)
AT (1) ATE383368T1 (bg)
AU (1) AU2002234161B2 (bg)
BG (1) BG66119B1 (bg)
CA (1) CA2427088C (bg)
CZ (1) CZ301360B6 (bg)
DE (1) DE60132366T2 (bg)
ES (1) ES2299528T3 (bg)
IL (1) IL155577A0 (bg)
NO (1) NO326288B1 (bg)
PL (1) PL206428B1 (bg)
PT (1) PT1366060E (bg)
WO (1) WO2002034791A2 (bg)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7544354B2 (en) * 2000-10-27 2009-06-09 Novartis Vaccines And Diagnostics Methods of protein purification and recovery
US20060035327A1 (en) * 2001-02-28 2006-02-16 Guangwen Wei Recombinant super-compound interferon and uses thereof
US20050079579A1 (en) * 2001-02-28 2005-04-14 Guangwen Wei Uses of spatial configuration to modulate protein function
CN1245215C (zh) * 2001-02-28 2006-03-15 四川省生物工程研究中心 重组高效复合干扰素用作乙型肝炎表面抗原和e抗原抑制剂
US6887462B2 (en) 2001-04-09 2005-05-03 Chiron Corporation HSA-free formulations of interferon-beta
US20050029194A1 (en) * 2003-08-07 2005-02-10 Hall David Bruce Method for removal of guanidine compound from aqueous media
US7585647B2 (en) * 2003-08-28 2009-09-08 Guangwen Wei Nucleic acid encoding recombinant interferon
WO2006134497A2 (en) * 2005-03-09 2006-12-21 Guangwen Wei Uses of recombinant super-compound interferons
US20090247421A1 (en) * 2005-03-23 2009-10-01 Egisto Boschetti Diverse chemical libraries bound to small particles with paramagnetic properties
EP1871726A2 (en) * 2005-03-23 2008-01-02 Bio-Rad Laboratories, Inc. Diverse chemical libraries bound to small particles with paramagnetic properties
JP2009502173A (ja) * 2005-07-29 2009-01-29 ノバルティス アーゲー インビトロでのタンパク質折りたたみのための方法およびシステム
JP2007126391A (ja) * 2005-11-02 2007-05-24 Sanyo Chem Ind Ltd タンパク質のリフォールディング剤
WO2007020886A1 (ja) 2005-08-16 2007-02-22 Sanyo Chemical Industries, Ltd. タンパク質のリフォールディング剤およびリフォールディング方法
JP4786303B2 (ja) * 2005-11-02 2011-10-05 三洋化成工業株式会社 タンパク質のリフォールディング剤
JP5274795B2 (ja) * 2006-07-27 2013-08-28 三洋化成工業株式会社 タンパク質のリフォールディング方法
KR20100019467A (ko) 2007-05-02 2010-02-18 암브룩스, 인코포레이티드 변형된 인터페론 베타 폴리펩티드 및 그의 용도
WO2009045553A1 (en) * 2007-10-05 2009-04-09 Barofold, Inc. High pressure treatment of aggregated interferons
KR101113495B1 (ko) 2008-01-23 2012-02-29 한미홀딩스 주식회사 인간 인터페론 베타의 정제방법
GB0821010D0 (en) * 2008-11-17 2008-12-24 Univ Warwick Plant development control composition
CN101885767B (zh) * 2010-06-13 2013-03-13 深圳科兴生物工程有限公司 一种取代单克隆抗体亲和层析的分离纯化重组人干扰素α1b的方法

Citations (2)

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US4462940A (en) * 1982-09-23 1984-07-31 Cetus Corporation Process for the recovery of human β-interferon-like polypeptides
US5702699A (en) * 1982-09-23 1997-12-30 Cetus Corporation Process for the recovery of lipophilic proteins

Family Cites Families (18)

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US5643566A (en) 1982-09-23 1997-07-01 Cetus Corporation Formulation processes for lipophilic proteins
US4992271A (en) 1982-09-23 1991-02-12 Cetus Corporation Formulation for lipophilic IL-2 proteins
US4816440A (en) 1985-09-26 1989-03-28 Cetus Corporation Stable formulation of biologically active proteins for parenteral injection
US5183746A (en) * 1986-10-27 1993-02-02 Schering Aktiengesellschaft Formulation processes for pharmaceutical compositions of recombinant β-
CA1294215C (en) 1986-10-27 1992-01-14 Ze'ev Shaked Pharmaceutical compositions of recombinant beta-interferon and formulation processes
US4894330A (en) 1986-12-23 1990-01-16 Cetus Corporation Purification of recombinant beta-interferon incorporating RP-HPLC
US4931543A (en) 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
US5162507A (en) 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US5004605A (en) * 1987-12-10 1991-04-02 Cetus Corporation Low pH pharmaceutical compositions of recombinant β-interferon
CA1340586C (en) 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
KR930006706B1 (ko) 1991-04-29 1993-07-23 주식회사 럭키 인간 인터페론 베타의 정제방법
DE4407325B4 (de) * 1994-03-04 2006-06-29 Basf Ag Verfahren zur Herstellung von 3-Aminomethyl-3,5,5-trimethylcyclohexylamin
CA2190502A1 (en) 1994-05-18 1995-11-23 Robert M. Platz Methods and compositions for the dry powder formulation of interferons
US5814485A (en) * 1995-06-06 1998-09-29 Chiron Corporation Production of interferon-β (IFN-β) in E. coli
BR9606270A (pt) 1996-12-18 1998-09-22 Univ Minas Gerais Processo para a produção da proteína do interferon beta-cis humano recombinante e proteína de interferon beta-cis humano recombinante
US20030190307A1 (en) * 1996-12-24 2003-10-09 Biogen, Inc. Stable liquid interferon formulations
US7544354B2 (en) * 2000-10-27 2009-06-09 Novartis Vaccines And Diagnostics Methods of protein purification and recovery
US6887462B2 (en) 2001-04-09 2005-05-03 Chiron Corporation HSA-free formulations of interferon-beta

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4462940A (en) * 1982-09-23 1984-07-31 Cetus Corporation Process for the recovery of human β-interferon-like polypeptides
US5702699A (en) * 1982-09-23 1997-12-30 Cetus Corporation Process for the recovery of lipophilic proteins

Also Published As

Publication number Publication date
PL206428B1 (pl) 2010-08-31
EP1366060A2 (en) 2003-12-03
US7544354B2 (en) 2009-06-09
US20090123424A1 (en) 2009-05-14
NO326288B1 (no) 2008-11-03
US20020137895A1 (en) 2002-09-26
BG107844A (bg) 2004-07-30
CN100493601C (zh) 2009-06-03
ATE383368T1 (de) 2008-01-15
AU2002234161B2 (en) 2006-10-19
PL365896A1 (en) 2005-01-10
DE60132366D1 (de) 2008-02-21
CN1479627A (zh) 2004-03-03
US20040115169A1 (en) 2004-06-17
NO20031839D0 (no) 2003-04-24
JP2004512343A (ja) 2004-04-22
CZ20031157A3 (en) 2004-03-17
ES2299528T3 (es) 2008-06-01
US8388942B2 (en) 2013-03-05
CZ301360B6 (cs) 2010-01-27
PT1366060E (pt) 2008-03-17
WO2002034791A2 (en) 2002-05-02
IL155577A0 (en) 2003-11-23
EP1366060B1 (en) 2008-01-09
CA2427088A1 (en) 2002-05-02
DE60132366T2 (de) 2009-01-29
JP3946139B2 (ja) 2007-07-18
WO2002034791A3 (en) 2003-10-09
CA2427088C (en) 2011-06-21
NO20031839L (no) 2003-05-28

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