BG49719A3 - Method for preparing of variants chirudine and their use - Google Patents
Method for preparing of variants chirudine and their useInfo
- Publication number
- BG49719A3 BG49719A3 BG082035A BG8203587A BG49719A3 BG 49719 A3 BG49719 A3 BG 49719A3 BG 082035 A BG082035 A BG 082035A BG 8203587 A BG8203587 A BG 8203587A BG 49719 A3 BG49719 A3 BG 49719A3
- Authority
- BG
- Bulgaria
- Prior art keywords
- chirudine
- variants
- preparing
- yeast
- anticoagulants
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 2
- 102000007625 Hirudins Human genes 0.000 abstract 1
- 108010007267 Hirudins Proteins 0.000 abstract 1
- 239000003146 anticoagulant agent Substances 0.000 abstract 1
- 229940127219 anticoagulant drug Drugs 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 abstract 1
- 229940006607 hirudin Drugs 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Pharmacology & Pharmacy (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Epidemiology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
- Cosmetics (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR868616723A FR2607517B2 (fr) | 1986-12-01 | 1986-12-01 | Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BG49719A3 true BG49719A3 (en) | 1992-01-15 |
Family
ID=9341393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BG082035A BG49719A3 (en) | 1986-12-01 | 1987-12-01 | Method for preparing of variants chirudine and their use |
Country Status (24)
| Country | Link |
|---|---|
| US (1) | US5650301A (xx) |
| EP (1) | EP0273800B1 (xx) |
| JP (1) | JP2567263B2 (xx) |
| KR (1) | KR960011918B1 (xx) |
| AT (1) | ATE73462T1 (xx) |
| AU (1) | AU617989B2 (xx) |
| BG (1) | BG49719A3 (xx) |
| CA (1) | CA1341416C (xx) |
| CS (1) | CS276245B6 (xx) |
| DE (1) | DE3777367D1 (xx) |
| DK (1) | DK173247B1 (xx) |
| ES (1) | ES2032465T3 (xx) |
| FI (1) | FI99211C (xx) |
| FR (1) | FR2607517B2 (xx) |
| GR (1) | GR3004556T3 (xx) |
| HU (1) | HU213871B (xx) |
| IE (1) | IE60544B1 (xx) |
| MC (1) | MC1884A1 (xx) |
| NO (1) | NO177500C (xx) |
| PL (1) | PL157254B1 (xx) |
| PT (1) | PT86233B (xx) |
| RO (1) | RO106890B1 (xx) |
| SU (1) | SU1687032A3 (xx) |
| ZA (1) | ZA879011B (xx) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2628429B1 (fr) * | 1988-03-08 | 1990-12-28 | Transgene Sa | Variants de l'hirudine, leurs utilisations et les procedes pour les obtenir |
| GB8817160D0 (en) * | 1988-07-19 | 1988-08-24 | Ciba Geigy Ag | Novel proteins |
| GB8817161D0 (en) * | 1988-07-19 | 1988-08-24 | Ciba Geigy Ag | Modified proteins |
| US5284768A (en) * | 1988-08-24 | 1994-02-08 | Sanofi | Signal peptide, DNA sequences coding for the latter, expression vectors carrying one of these sequences, gram-negative bacteria transformed by these vectors, and process for the periplasmic production of a polypeptide |
| FR2645174B1 (fr) * | 1989-03-31 | 1994-01-07 | Transgene Sa | Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant |
| US5879926A (en) * | 1989-03-31 | 1999-03-09 | Transgene S.A. | Yeast strains for the production of mature heterologous proteins, especially hirudin |
| FR2645175B1 (fr) * | 1989-03-31 | 1994-02-18 | Transgene Sa | Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche |
| FR2646437B1 (fr) * | 1989-04-28 | 1991-08-30 | Transgene Sa | Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant |
| FR2656531B1 (fr) * | 1989-12-29 | 1992-04-24 | Sanofi Sa | Promoteur artificiel pour l'expression de proteines dans le levure. |
| US5118790A (en) * | 1990-07-24 | 1992-06-02 | Sri International | Analogs of hirudin |
| WO1992008736A1 (fr) * | 1990-11-08 | 1992-05-29 | Nippon Mining Company Limited | Mutant d'hirudine, sa production, anticoagulant, vecteur secretoire, microorganisme transforme par ledit vecteur et production d'un produit a partir dudit microorganisme |
| US5837808A (en) * | 1991-08-20 | 1998-11-17 | Baxter International Inc. | Analogs of hirudin |
| US5407822A (en) * | 1991-10-02 | 1995-04-18 | Sanofi | Artificial promoter for the expression of proteins in yeast |
| WO2020210965A1 (zh) * | 2019-04-16 | 2020-10-22 | 广东双骏生物科技有限公司 | 水蛭素突变体的提取纯化方法及其应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1341417C (fr) * | 1984-03-27 | 2003-01-21 | Paul Tolstoshev | Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine |
| ATE64956T1 (de) * | 1984-06-14 | 1991-07-15 | Ciba Geigy Ag | Verfahren zur herstellung von thrombininhibitoren. |
| DE3429430A1 (de) * | 1984-08-10 | 1986-02-20 | Hoechst Ag, 6230 Frankfurt | Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens |
| DE3445517C2 (de) * | 1984-12-13 | 1993-11-18 | Ciba Geigy | Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins |
| FR2593518B1 (fr) * | 1985-05-02 | 1989-09-08 | Transgene Sa | Vecteurs d'expression et de secretion de l'hirudine par les levures transformees |
| DE3689525D1 (de) * | 1985-07-17 | 1994-02-24 | Hoechst Ag | Neue Polypeptide mit blutgerinnungshemmender Wirkung, Verfahren zu deren Herstellung bzw. Gewinnung, deren Verwendung und diese enthaltende Mittel. |
-
1986
- 1986-12-01 FR FR868616723A patent/FR2607517B2/fr not_active Expired
-
1987
- 1987-11-25 NO NO874905A patent/NO177500C/no not_active IP Right Cessation
- 1987-11-27 IE IE323987A patent/IE60544B1/en not_active IP Right Cessation
- 1987-11-27 AU AU81880/87A patent/AU617989B2/en not_active Expired
- 1987-11-27 PT PT86233A patent/PT86233B/pt unknown
- 1987-11-30 DK DK198706276A patent/DK173247B1/da not_active IP Right Cessation
- 1987-11-30 CA CA000553153A patent/CA1341416C/fr not_active Expired - Fee Related
- 1987-11-30 RO RO130679A patent/RO106890B1/ro unknown
- 1987-11-30 SU SU874203831A patent/SU1687032A3/ru active
- 1987-11-30 EP EP87402696A patent/EP0273800B1/fr not_active Expired - Lifetime
- 1987-11-30 DE DE8787402696T patent/DE3777367D1/de not_active Expired - Lifetime
- 1987-11-30 AT AT87402696T patent/ATE73462T1/de not_active IP Right Cessation
- 1987-11-30 ES ES198787402696T patent/ES2032465T3/es not_active Expired - Lifetime
- 1987-12-01 FI FI875295A patent/FI99211C/fi not_active IP Right Cessation
- 1987-12-01 PL PL1987269164A patent/PL157254B1/pl unknown
- 1987-12-01 CS CS878732A patent/CS276245B6/cs not_active IP Right Cessation
- 1987-12-01 JP JP62305504A patent/JP2567263B2/ja not_active Expired - Lifetime
- 1987-12-01 HU HU875394A patent/HU213871B/hu unknown
- 1987-12-01 MC MC871932A patent/MC1884A1/xx unknown
- 1987-12-01 BG BG082035A patent/BG49719A3/xx unknown
- 1987-12-01 ZA ZA879011A patent/ZA879011B/xx unknown
- 1987-12-01 KR KR1019870013708A patent/KR960011918B1/ko not_active IP Right Cessation
-
1992
- 1992-05-12 GR GR920400903T patent/GR3004556T3/el unknown
-
1995
- 1995-06-07 US US08/480,511 patent/US5650301A/en not_active Expired - Lifetime
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BG49719A3 (en) | Method for preparing of variants chirudine and their use | |
| ES8802402A1 (es) | Un metodo para producir precursores de insulina humana. | |
| YU45871B (sh) | Polipeptid sa humanim imunointerferonskim (ifn-gama) svojstvima | |
| ES2156852T3 (es) | Citoquina mamifera, il-11. | |
| ATE130031T1 (de) | Verwendungen viraler enzyme. | |
| BG60053B2 (bg) | Метод за повишаване способността за получаване на антибиотик | |
| GR3026114T3 (en) | Methods for culturing HCV in B- or T-lymphocyte cell lines | |
| FI885679A (fi) | Samuttryckning i eukariotiska celler. | |
| JPS57105191A (en) | Production of human urogastrone | |
| BG36940A3 (en) | Method for preparing d- lactic acid | |
| ES8502160A1 (es) | Un metodo de producir inosina y-o guanosina | |
| DK92784D0 (da) | Dna-struktur til anvendelse ved fremstilling af insulin | |
| GB1491816A (en) | Urokinase production | |
| BG44208A3 (en) | Method for preparation of enduracydine | |
| DE3650445D1 (de) | Verfahren um 1-sesamin zu produzieren mittels Pflanenzellkulturen. | |
| JPS5668388A (en) | Production of cell body of spirulina algae | |
| EP0286688A4 (en) | SUPPORT FOR CELL CULTURE. | |
| DK142380A (da) | Fremgangsmaade til fremstilling af pentadecapeptider | |
| JPS56154990A (en) | Preparation of l-aminoacid oxidase | |
| JPS5432687A (en) | Cultivation of yeasts in high concentration | |
| JPS5437876A (en) | Separation of yeast variant for brewery having strong alcohol-resistance using cytlytic enzyme effective to living yeast cell | |
| BG36046A3 (en) | Method for cultivating basid | |
| NO874391L (no) | Nye gjaerekspresjonsvektorer for ifn-omega samt fremgangsmaate for fremstilling og anvendelse derav. | |
| GB1151236A (en) | Method of preparing High Activity Protease | |
| AT339848B (de) | Fermenter zur zuchtung von mikroorganismen, insbesondere von backhefe |