BG101556A - Нови производни на стафилокиназата - Google Patents
Нови производни на стафилокиназатаInfo
- Publication number
- BG101556A BG101556A BG101556A BG10155697A BG101556A BG 101556 A BG101556 A BG 101556A BG 101556 A BG101556 A BG 101556A BG 10155697 A BG10155697 A BG 10155697A BG 101556 A BG101556 A BG 101556A
- Authority
- BG
- Bulgaria
- Prior art keywords
- staphylokinase
- fragment
- dna fragment
- mutagenesis
- vitro
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
- C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Hematology (AREA)
- Pharmacology & Pharmacy (AREA)
- Diabetes (AREA)
- Animal Behavior & Ethology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Производните на стафилокиназата се получават чрезизготвяне на ДНК фрагмент, включващ поне част от кодиращата последователност на стафилокиназата, която допринася за нейната биологична активност, за представянето in vitro сайт-насочена мутагенеза върху ДНК фрагмент за заместване на един или повече кодони за природни аминокиселини с кодон за друга аминокиселина, за клонирането на мутиралия ДНК фрагмент в подходящ вектор, за трансформацията или трансфекция на подходяща гостоприемникова клетка с вектора и за култивирането на гостоприемниковата клетка при условия, подходящи за експресия на ДНК фрагмента. За предпочитане, ДНК фрагментът е 453 bp EcoRI-HindIII от плазмида pMEX602SakB, като in vitro мутагенезата се осъществява с олигонуклеотиднасочена мутагенезисна система с помощта на плазмида pMa/c и дефицитния Е.coli щам WK6MutS, и мутиралият ДНК фрагмент е експресиран в Е.coli щам WK6. Изобретението се отнася също и до състави, които съдържат поне едно от производните на стафилокиназата заедно с подходящ пълнител за лечение на артериални тромбози.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP95200023A EP0721982A1 (en) | 1995-01-06 | 1995-01-06 | New staphylokinase derivatives |
| US08/371,505 US5695754A (en) | 1995-01-06 | 1995-01-11 | Staphylokinase derivatives |
| EP95201531A EP0721013B1 (en) | 1995-01-06 | 1995-06-09 | New staphylokinase derivatives |
| US49909295A | 1995-07-06 | 1995-07-06 | |
| JP7299781A JPH08289790A (ja) | 1995-01-06 | 1995-11-17 | 新規なスタフィロキナーゼ誘導体 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BG101556A true BG101556A (bg) | 1998-02-27 |
| BG64344B1 BG64344B1 (bg) | 2004-10-29 |
Family
ID=27514236
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BG101556A BG64344B1 (bg) | 1995-01-06 | 1997-06-03 | Нови производни на стафилокиназата |
Country Status (22)
| Country | Link |
|---|---|
| EP (1) | EP0793723B1 (bg) |
| JP (1) | JPH08289790A (bg) |
| CN (1) | CN1154729C (bg) |
| AT (1) | ATE306550T1 (bg) |
| AU (1) | AU705119B2 (bg) |
| BG (1) | BG64344B1 (bg) |
| BR (1) | BR9606724A (bg) |
| CA (1) | CA2206479A1 (bg) |
| CZ (1) | CZ290685B6 (bg) |
| DE (1) | DE69635269T2 (bg) |
| EA (1) | EA000121B1 (bg) |
| EE (1) | EE9700150A (bg) |
| ES (1) | ES2248805T3 (bg) |
| FI (1) | FI972862A (bg) |
| HU (1) | HUP9802915A3 (bg) |
| NO (1) | NO973083L (bg) |
| NZ (1) | NZ298801A (bg) |
| PL (1) | PL184348B1 (bg) |
| RO (1) | RO116969B1 (bg) |
| SK (1) | SK89297A3 (bg) |
| TR (1) | TR199700600T1 (bg) |
| WO (1) | WO1996021016A2 (bg) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5951980A (en) * | 1995-01-06 | 1999-09-14 | Leuven Research & Development Vzw | Identification, production and use of new staphylokinase derivatives with reduced immunogenicity |
| EP1277762B1 (en) * | 1998-10-30 | 2008-03-26 | Novozymes A/S | Low allergenic protein variants |
| US6686164B1 (en) | 1998-10-30 | 2004-02-03 | Novozymes A/S | Low allergenic protein variants |
| CN100342007C (zh) * | 2000-01-28 | 2007-10-10 | 复旦大学 | 一种新型重组葡激酶及其制备方法 |
| US6673580B2 (en) | 2000-10-27 | 2004-01-06 | Genentech, Inc. | Identification and modification of immunodominant epitopes in polypeptides |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU669149B2 (en) * | 1991-12-30 | 1996-05-30 | Thromb-X N.V. | Expression signal-peptide-free staphylokinases |
-
1995
- 1995-11-17 JP JP7299781A patent/JPH08289790A/ja active Pending
-
1996
- 1996-01-03 CN CNB961913509A patent/CN1154729C/zh not_active Expired - Lifetime
- 1996-01-03 SK SK892-97A patent/SK89297A3/sk unknown
- 1996-01-03 BR BR9606724A patent/BR9606724A/pt not_active Application Discontinuation
- 1996-01-03 AU AU44377/96A patent/AU705119B2/en not_active Ceased
- 1996-01-03 RO RO97-01250A patent/RO116969B1/ro unknown
- 1996-01-03 CZ CZ19972104A patent/CZ290685B6/cs not_active IP Right Cessation
- 1996-01-03 TR TR97/00600T patent/TR199700600T1/xx unknown
- 1996-01-03 CA CA002206479A patent/CA2206479A1/en not_active Abandoned
- 1996-01-03 EE EE9700150A patent/EE9700150A/xx unknown
- 1996-01-03 NZ NZ298801A patent/NZ298801A/xx not_active IP Right Cessation
- 1996-01-03 EP EP96900577A patent/EP0793723B1/en not_active Expired - Lifetime
- 1996-01-03 WO PCT/EP1996/000081 patent/WO1996021016A2/en active IP Right Grant
- 1996-01-03 ES ES96900577T patent/ES2248805T3/es not_active Expired - Lifetime
- 1996-01-03 EA EA199700073A patent/EA000121B1/ru not_active IP Right Cessation
- 1996-01-03 HU HU9802915A patent/HUP9802915A3/hu unknown
- 1996-01-03 DE DE69635269T patent/DE69635269T2/de not_active Expired - Fee Related
- 1996-01-03 AT AT96900577T patent/ATE306550T1/de not_active IP Right Cessation
-
1997
- 1997-06-03 BG BG101556A patent/BG64344B1/bg unknown
- 1997-07-02 NO NO973083A patent/NO973083L/no not_active Application Discontinuation
- 1997-07-04 FI FI972862A patent/FI972862A/fi not_active IP Right Cessation
- 1997-07-04 PL PL96321181A patent/PL184348B1/pl unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HUP9802915A3 (en) | 2001-02-28 |
| WO1996021016A2 (en) | 1996-07-11 |
| ES2248805T3 (es) | 2006-03-16 |
| NO973083D0 (no) | 1997-07-02 |
| MX9705041A (es) | 1998-07-31 |
| EA000121B1 (ru) | 1998-08-27 |
| CZ210497A3 (en) | 1997-11-12 |
| EA199700073A1 (ru) | 1997-12-30 |
| TR199700600T1 (xx) | 1998-01-21 |
| NZ298801A (en) | 2000-01-28 |
| BG64344B1 (bg) | 2004-10-29 |
| SK89297A3 (en) | 1998-05-06 |
| AU4437796A (en) | 1996-07-24 |
| CN1168156A (zh) | 1997-12-17 |
| WO1996021016A3 (en) | 1996-09-12 |
| HUP9802915A2 (hu) | 1999-03-29 |
| AU705119B2 (en) | 1999-05-13 |
| ATE306550T1 (de) | 2005-10-15 |
| RO116969B1 (ro) | 2001-08-30 |
| PL321181A1 (en) | 1997-11-24 |
| PL184348B1 (pl) | 2002-10-31 |
| EP0793723B1 (en) | 2005-10-12 |
| NO973083L (no) | 1997-08-04 |
| FI972862A0 (fi) | 1997-07-04 |
| DE69635269D1 (de) | 2006-02-23 |
| DE69635269T2 (de) | 2006-06-14 |
| FI972862A (fi) | 1997-09-03 |
| CZ290685B6 (cs) | 2002-09-11 |
| EP0793723A2 (en) | 1997-09-10 |
| EE9700150A (et) | 1997-12-15 |
| JPH08289790A (ja) | 1996-11-05 |
| BR9606724A (pt) | 1998-01-13 |
| CA2206479A1 (en) | 1996-07-11 |
| CN1154729C (zh) | 2004-06-23 |
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