MX9705041A - Nuevos derivados de estafilocinasa. - Google Patents
Nuevos derivados de estafilocinasa.Info
- Publication number
- MX9705041A MX9705041A MX9705041A MX9705041A MX9705041A MX 9705041 A MX9705041 A MX 9705041A MX 9705041 A MX9705041 A MX 9705041A MX 9705041 A MX9705041 A MX 9705041A MX 9705041 A MX9705041 A MX 9705041A
- Authority
- MX
- Mexico
- Prior art keywords
- dna fragment
- directed mutagenesis
- plasmid
- host cell
- staphylokinase
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
- C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Hematology (AREA)
- Pharmacology & Pharmacy (AREA)
- Diabetes (AREA)
- Animal Behavior & Ethology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
La presente invencion se refiere a un método para producir los derivados de la invencion preparando un fragmento de ADN que comprende al menos la parte de la secuencia de codificacion de la estafilocinasa que proporciona su actividad biologica; efectuar la mutagénesis dirigida al sitio in vitro sobre el fragmento de ADN para reemplazar uno o más codones para los aminoácidos del tipo silvestre por un codon para otro aminoácido; clonar el fragmento de ADN mutado en un vector adecuado; transformar o transfectar una célula huésped adecuada con el vector; y cultivar la célula huésped bajo condiciones adecuadas para expresar el fragmento de ADN. Preferiblemente el fragmento de ADN es un fragmento EcoRI-HindIII de 453 pb del plásmido pMEX602SakB, la mutagénesis dirigida al sitio in vitro es efectuada por un sistema de mutagénesis dirigida al oligonucleotido utilizando el plásmido pMa/c y la cepa WK6MutS del E. coli deficiente en la reparacion, y el fragmento de ADN es expresado en la cepa WK6 del E. coli. La invencion también se refiere a composiciones farmacéuticas que comprenden al menos uno de los derivados de estafilocinasa de acuerdo con la invencion junto con un excipiente adecuado, para el tratamiento de la trombosis arterial.
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP95200023A EP0721982A1 (en) | 1995-01-06 | 1995-01-06 | New staphylokinase derivatives |
| EP95200023 | 1995-01-06 | ||
| US08/371,505 US5695754A (en) | 1995-01-06 | 1995-01-11 | Staphylokinase derivatives |
| US08371505 | 1995-01-11 | ||
| EP95201531A EP0721013B1 (en) | 1995-01-06 | 1995-06-09 | New staphylokinase derivatives |
| EP95201531 | 1995-06-09 | ||
| US49909295A | 1995-07-06 | 1995-07-06 | |
| US499092 | 1995-07-06 | ||
| JP7/299781 | 1995-11-17 | ||
| JP7299781A JPH08289790A (ja) | 1995-01-06 | 1995-11-17 | 新規なスタフィロキナーゼ誘導体 |
| JP7-299781 | 1995-11-17 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX9705041A true MX9705041A (es) | 1998-07-31 |
| MXPA97005041A MXPA97005041A (es) | 1998-11-09 |
Family
ID=
Also Published As
| Publication number | Publication date |
|---|---|
| BG101556A (en) | 1998-02-27 |
| CN1168156A (zh) | 1997-12-17 |
| FI972862A (fi) | 1997-09-03 |
| EE9700150A (et) | 1997-12-15 |
| SK89297A3 (en) | 1998-05-06 |
| WO1996021016A2 (en) | 1996-07-11 |
| EP0793723A2 (en) | 1997-09-10 |
| EA000121B1 (ru) | 1998-08-27 |
| CZ290685B6 (cs) | 2002-09-11 |
| CA2206479A1 (en) | 1996-07-11 |
| HUP9802915A2 (hu) | 1999-03-29 |
| CZ210497A3 (en) | 1997-11-12 |
| BG64344B1 (bg) | 2004-10-29 |
| NZ298801A (en) | 2000-01-28 |
| PL184348B1 (pl) | 2002-10-31 |
| AU705119B2 (en) | 1999-05-13 |
| PL321181A1 (en) | 1997-11-24 |
| ES2248805T3 (es) | 2006-03-16 |
| DE69635269T2 (de) | 2006-06-14 |
| CN1154729C (zh) | 2004-06-23 |
| JPH08289790A (ja) | 1996-11-05 |
| AU4437796A (en) | 1996-07-24 |
| EP0793723B1 (en) | 2005-10-12 |
| HUP9802915A3 (en) | 2001-02-28 |
| NO973083L (no) | 1997-08-04 |
| WO1996021016A3 (en) | 1996-09-12 |
| EA199700073A1 (ru) | 1997-12-30 |
| NO973083D0 (no) | 1997-07-02 |
| RO116969B1 (ro) | 2001-08-30 |
| DE69635269D1 (de) | 2006-02-23 |
| ATE306550T1 (de) | 2005-10-15 |
| BR9606724A (pt) | 1998-01-13 |
| TR199700600T1 (xx) | 1998-01-21 |
| FI972862A0 (fi) | 1997-07-04 |
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