JPS6439999A - Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmid - Google Patents
Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmidInfo
- Publication number
- JPS6439999A JPS6439999A JP19719787A JP19719787A JPS6439999A JP S6439999 A JPS6439999 A JP S6439999A JP 19719787 A JP19719787 A JP 19719787A JP 19719787 A JP19719787 A JP 19719787A JP S6439999 A JPS6439999 A JP S6439999A
- Authority
- JP
- Japan
- Prior art keywords
- myc
- variant
- gene
- myc protein
- recombinant plasmid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE:To readily and stably obtain the titled variant myc protein useful in remedy, diagnosis, etc., for cancer, by cultivating a transformant obtained by introducing a plasmid containing a specific myc gene to a host cell in a culture medium. CONSTITUTION:A human placenta cDNA library, etc., is selected to afford C-myc cDNA coding myc protein which is a human carcinogenic gene product. Then cDNA codon coding a constitutional amino acid of the C-myc protein is converted into other codon coding one or more amino acid for obtaining stable myc protein by site-specific mutagenesis method to give a variant myc gene (A) having a base sequence expressed by the formula. Then a vector for express of the component A is constituted to afford a recombinant plasmid (B). Further transformant obtained by introducing the component B into a human cell, etc., is cultivated in a culture medium and the resultant cultivation product is separated and purified according to Rosenberg method to provide the variant myc protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19719787A JPS6439999A (en) | 1987-08-06 | 1987-08-06 | Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19719787A JPS6439999A (en) | 1987-08-06 | 1987-08-06 | Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6439999A true JPS6439999A (en) | 1989-02-10 |
Family
ID=16370426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19719787A Pending JPS6439999A (en) | 1987-08-06 | 1987-08-06 | Stabilization of myc protein, variant myc gene, recombinant plasmid containing said gene and production of variant myc protein using transformant by said recombinant plasmid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6439999A (en) |
-
1987
- 1987-08-06 JP JP19719787A patent/JPS6439999A/en active Pending
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