AU2017234815B2 - Cell sorting using a high throughput fluorescence flow cytometer - Google Patents

Cell sorting using a high throughput fluorescence flow cytometer Download PDF

Info

Publication number
AU2017234815B2
AU2017234815B2 AU2017234815A AU2017234815A AU2017234815B2 AU 2017234815 B2 AU2017234815 B2 AU 2017234815B2 AU 2017234815 A AU2017234815 A AU 2017234815A AU 2017234815 A AU2017234815 A AU 2017234815A AU 2017234815 B2 AU2017234815 B2 AU 2017234815B2
Authority
AU
Australia
Prior art keywords
particle
fluorescence
cell
radiation
data
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2017234815A
Other languages
English (en)
Other versions
AU2017234815A1 (en
Inventor
Eric Diebold
Jonathan Lin
Keegan Owsley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of AU2017234815A1 publication Critical patent/AU2017234815A1/en
Assigned to BECTON, DICKINSON AND COMPANY reassignment BECTON, DICKINSON AND COMPANY Request for Assignment Assignors: BD BIOSCIENCES
Application granted granted Critical
Publication of AU2017234815B2 publication Critical patent/AU2017234815B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1447—Spatial selection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2017234815A 2016-03-17 2017-03-17 Cell sorting using a high throughput fluorescence flow cytometer Active AU2017234815B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662309806P 2016-03-17 2016-03-17
US62/309,806 2016-03-17
PCT/US2017/022936 WO2017161247A1 (en) 2016-03-17 2017-03-17 Cell sorting using a high throughput fluorescence flow cytometer

Publications (2)

Publication Number Publication Date
AU2017234815A1 AU2017234815A1 (en) 2018-10-04
AU2017234815B2 true AU2017234815B2 (en) 2022-11-03

Family

ID=58428427

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2017234815A Active AU2017234815B2 (en) 2016-03-17 2017-03-17 Cell sorting using a high throughput fluorescence flow cytometer

Country Status (10)

Country Link
US (7) US10324019B2 (https=)
EP (2) EP3430376B8 (https=)
JP (3) JP7130562B2 (https=)
KR (3) KR102527830B1 (https=)
CN (1) CN109564151B (https=)
AU (1) AU2017234815B2 (https=)
BR (1) BR112018068866B1 (https=)
CA (1) CA3018065A1 (https=)
WO (1) WO2017161247A1 (https=)
ZA (1) ZA201806386B (https=)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
EP2943778B1 (en) 2013-01-09 2020-10-28 The Regents of The University of California Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation
EP3120130B1 (en) 2014-03-18 2023-07-26 The Regents of the University of California Parallel flow cytometer using radiofrequency mulitplexing, and method
CN105987870A (zh) * 2015-02-10 2016-10-05 博奥生物集团有限公司 一种流式细胞分选系统及其聚焦检测方法及其流体芯片
EP3350542B1 (en) * 2015-09-17 2022-07-27 Technion Research & Development Foundation Limited Reflectance confocal microscopy of blood cells
CN108474728B (zh) 2015-10-13 2022-04-26 贝克顿·迪金森公司 多模态荧光成像流式细胞术系统
JP7130562B2 (ja) 2016-03-17 2022-09-05 ベクトン・ディキンソン・アンド・カンパニー ハイスループット蛍光フローサイトメーターを用いる細胞選別
CN109477784B (zh) 2016-05-12 2022-04-01 Bd生物科学公司 具有增强图像分辨率的荧光成像流式细胞术
FI3469338T3 (fi) * 2016-06-10 2025-05-06 Univ California Kuvapohjaisia solunlajittelujärjestelmiä ja -menetelmiä
CN109477785B (zh) 2016-09-13 2022-09-27 贝克顿·迪金森公司 具有光学均衡的流式细胞仪
EP3579973A4 (en) 2017-02-07 2020-07-29 Nodexus Inc. MICROFLUIDIC SYSTEM WITH COMBINED ELECTRICAL AND OPTICAL DETECTION FOR HIGH-PRECISION PARTICLE SORTING AND RELATED PROCESSES
US20180292662A1 (en) * 2017-04-05 2018-10-11 Osela Inc. Rectangular beam shaper having monolithic body of refractive material
WO2019012776A1 (ja) * 2017-07-11 2019-01-17 浜松ホトニクス株式会社 試料観察装置及び試料観察方法
US10636182B2 (en) 2017-07-18 2020-04-28 Becton, Dickinson And Company Dynamic interactive display of multi-parameter quantitative biological data
CN110770571B (zh) 2017-07-18 2022-09-20 贝克顿·迪金森公司 多参数定量生物学数据的动态交互显示
US10593082B2 (en) 2017-07-18 2020-03-17 Becton, Dickinson And Company Dynamic display of multi-parameter quantitative biological data
US11099129B2 (en) 2017-09-14 2021-08-24 Brown University Spatially resolved standoff trace chemical sensing using backwards transient absorption spectroscopy
US10466173B2 (en) * 2017-10-06 2019-11-05 Wyatt Technology Corporation Optical flow cell assembly incorporating a replaceable transparent flow cell
US10267723B1 (en) 2018-01-23 2019-04-23 Cbrn International, Ltd. Bioaerosol particle detector
WO2019161406A1 (en) * 2018-02-16 2019-08-22 The Regents Of The University Of California Systems, devices and methods for three-dimensional imaging of moving particles
CN112912513A (zh) * 2018-08-28 2021-06-04 贝克顿迪金森公司 使用糖类结合试剂和膜透过性试剂进行样品多重化
EP3850080B1 (en) * 2018-09-14 2025-11-05 The Regents of the University of California Cell sorting device and method
US11841311B2 (en) * 2018-11-19 2023-12-12 Samsung Electronics Co., Ltd. Multimodal dust sensor
EP3908824B1 (en) * 2019-01-11 2025-09-10 Becton, Dickinson and Company Optimized sorting gates
EP3942279A4 (en) 2019-03-21 2022-12-28 Becton, Dickinson and Company LIGHT DETECTION SYSTEMS AND METHODS OF USE THEREOF
JP7416821B2 (ja) * 2019-03-22 2024-01-17 ベクトン・ディキンソン・アンド・カンパニー 高周波多重励起データを使用した蛍光イメージングのスペクトルアンミックス
EP3948221A4 (en) 2019-03-27 2022-12-21 Becton, Dickinson and Company FREQUENCY CODED IMAGE-BASED CELL SORTING SYSTEMS AND METHODS OF USE THEREOF
JP7466563B2 (ja) * 2019-03-29 2024-04-15 ベクトン・ディキンソン・アンド・カンパニー 粒子の区別に使用するためのパラメータ
JP7504127B2 (ja) * 2019-05-14 2024-06-21 ベクトン・ディキンソン・アンド・カンパニー イメージングフローサイトメトリーのための位相キャリブレーション
IL287195B2 (en) 2019-05-30 2025-07-01 Becton Dickinson Co Phase correction of multiplexed radio frequency signals
EP3997439A4 (en) 2019-07-10 2023-07-19 Becton, Dickinson and Company Reconfigurable integrated circuits for adjusting cell sorting classification
EP4090764A1 (en) 2020-01-13 2022-11-23 Becton, Dickinson and Company Cell capture using du-containing oligonucleotides
JP7663587B2 (ja) 2020-01-31 2025-04-16 ベクトン・ディキンソン・アンド・カンパニー 訓練ゲートを調整してフローサイトメータデータに適応させるための方法およびシステム
US11674877B2 (en) * 2020-02-03 2023-06-13 LASE Innovation Inc. Apparatus and method for cyclic flow cytometry using particularized cell identification
EP4143536B1 (en) * 2020-04-29 2025-08-20 Becton, Dickinson and Company Methods for modulation and synchronous detection in a flow cytometer and systems for same
US11674879B2 (en) 2020-05-06 2023-06-13 Becton, Dickinson And Company Methods and systems for characterizing spillover spreading in flow cytometer data
AU2021275676A1 (en) 2020-05-19 2022-12-08 Becton, Dickinson And Company Methods for modulating an intensity profile of a laser beam and systems for same
JP7451767B2 (ja) * 2020-05-20 2024-03-18 ワイエスアイ，インコーポレイティド 拡張した立体角の濁度センサ
WO2021257144A1 (en) 2020-06-19 2021-12-23 Becton, Dickinson And Company Flow cytometer with adjustable positional offset sort deflection plates and methods of using the same
WO2021262359A1 (en) 2020-06-24 2021-12-30 Becton, Dickinson And Company Flow cytometric droplet dispensing systems and methods for using the same
EP4172592B1 (en) 2020-06-26 2025-07-16 Becton, Dickinson and Company Dual excitation beams for irradiating a sample in a flow stream and methods for using same
EP4610628A3 (en) 2020-09-22 2025-11-19 Becton, Dickinson And Company Methods for continuous measurement of baseline noise in a flow cytometer and systems for same
US20220120658A1 (en) 2020-10-20 2022-04-21 Becton, Dickinson And Company Flow cytometers including tilted beam shaping optical components, and methods of using the same
GB2600719B (en) * 2020-11-05 2023-10-11 Oxford Nanoimaging Ltd Fluorescence assay
JP7766690B2 (ja) 2020-11-19 2025-11-10 ベクトン・ディキンソン・アンド・カンパニー 機械学習分析のためのサイトメトリックデータの最適なスケーリング方法及びそのシステム
US11959849B2 (en) 2021-01-13 2024-04-16 Becton, Dickinson And Company Flow cytometers including light collection enhancers, and methods of using the same
WO2022164672A1 (en) * 2021-01-27 2022-08-04 Becton, Dickinson And Company Flow cytometers including fiber optic light collectors, and methods of use thereof
EP4288757A4 (en) 2021-02-04 2024-07-31 Becton, Dickinson and Company LIGHT-INTEGRATED INTERROGATION MODULES AND METHODS OF USE THEREOF
US12379301B2 (en) 2021-03-24 2025-08-05 Becton, Dickinson And Company Closed-system sorting flow cytometer adapters and methods of use thereof
US12313524B2 (en) 2021-05-14 2025-05-27 Becton, Dickinson And Company Systems for detecting light by spectral discrimination and methods for using same
CN113252142B (zh) * 2021-05-18 2022-04-19 西南科技大学 一种密闭容器中白酒液位的非接触式测量系统及方法
JP2024530407A (ja) * 2021-07-13 2024-08-21 ベクトン・ディキンソン・アンド・カンパニー フローサイトメータ内の光信号についての光検出器利得補正係数を決定するための方法
US12135274B2 (en) 2021-08-10 2024-11-05 Becton, Dickinson And Company Outlet fittings for reducing bubbles at the interface with a flow cell, and flow cytometers and methods using the same
US12467845B2 (en) 2021-08-10 2025-11-11 Becton, Dickinson And Company Clamps for operably coupling an optical component to a mounting block, and methods and systems for using the same
AU2022352559A1 (en) 2021-09-21 2024-03-21 Becton, Dickinson And Company Baseline restoration circuit
IL311201A (en) 2021-11-17 2024-05-01 Becton Dickinson Co Methods for dynamic real-time adjustment of a data acquisition parameter in a flow cytometer
EP4437324A4 (en) 2021-11-24 2025-03-05 Becton, Dickinson and Company Integrated flow cytometry data quality control
CN116337699B (zh) * 2021-12-24 2025-12-12 晋城三赢精密电子有限公司 颗粒物光学检测系统及方法
CN114441418A (zh) * 2022-01-28 2022-05-06 天津凌视科技有限公司 用于高速流动微粒的成像系统、成像方法及可读存储介质
KR102553905B1 (ko) * 2022-08-24 2023-07-10 주식회사 팍스웰 세포 분석을 위한 바이오 칩
JP2026507551A (ja) * 2023-02-14 2026-03-04 ベクトン・ディキンソン・アンド・カンパニー サンプルの粒子を検出するためのデータフィルタを決定するための方法、並びにこれを用いるシステム及び方法
US20240312191A1 (en) 2023-03-14 2024-09-19 Becton, Dickinson And Company Methods for determining image filters for classifying particles of a sample and systems and methods for using same
US20240344983A1 (en) 2023-03-30 2024-10-17 Becton, Dickinson And Company Methods and systems for visualizing spectral signatures
US20240377307A1 (en) 2023-05-09 2024-11-14 Becton, Dickinson And Company Methods and systems for classifying analyte data into clusters
US20240377311A1 (en) 2023-05-09 2024-11-14 Becton, Dickinson And Company Methods for image-based detection and sorting and systems for same
US20240377325A1 (en) 2023-05-09 2024-11-14 Becton, Dickinson And Company Methods for assessing cell mitochondrial morphology and systems for same
DE102023113321A1 (de) 2023-05-22 2024-11-28 Kurz Elektronik Gmbh Vorrichtung zur Erfassung und Auswertung von Partikel
US20250085212A1 (en) 2023-09-07 2025-03-13 Becton, Dickinson And Company Integrated Optofluidic Interrogation Modules, Flow Cytometers Including the Same, and Methods of Use Thereof
US20250137905A1 (en) 2023-10-27 2025-05-01 Becton, Dickinson And Company Fluid Supply Systems Having a Flow Control Circuit and Single Fluidic Connection, and Methods of Use Thereof
WO2025137955A1 (zh) * 2023-12-27 2025-07-03 深圳华大智造科技股份有限公司 光学整形系统及相关设备与方法
US20250237657A1 (en) 2024-01-18 2025-07-24 Becton, Dickinson And Company Methods for spectrally resolving fluorophores of a sample by generalized least squares and systems for same
US20250244333A1 (en) 2024-01-31 2025-07-31 Becton, Dickinson And Company Cell viability compensation (cvc) beads for flow cytometry applications and methods of using the same
US20250297940A1 (en) 2024-03-25 2025-09-25 Becton, Dickinson And Company Methods and systems for classifying analyte data
US20250308080A1 (en) 2024-04-02 2025-10-02 Becton, Dickinson And Company Methods for label-free cell sorting and systems for same
US20250314573A1 (en) 2024-04-05 2025-10-09 Becton, Dickinson And Company Gate/population naming in flow cytometry data analysis based on geometry and data distribution
US20250314577A1 (en) 2024-04-09 2025-10-09 Becton, Dickinson And Company Methods for assessing cell nuclei morphology and systems for same
US20250321179A1 (en) 2024-04-10 2025-10-16 Becton, Dickinson And Company Quantitative flow cytometry light scatter detector alignment
US20250341455A1 (en) 2024-05-03 2025-11-06 Becton, Dickinson And Company Flow cells having an optimized flow channel geometry, flow cytometers including the same, and methods of use thereof
US20250369861A1 (en) 2024-05-31 2025-12-04 Becton, Dickinson And Company Methods and systems for singlet discrimination in flow cytometry data and systems for same
US20250377284A1 (en) 2024-06-11 2025-12-11 Becton, Dickinson And Company Flow cells having optimized high voltage electrodes, flow cytometers including the same, and methods of use thereof
WO2025257775A1 (en) 2024-06-13 2025-12-18 Nippon Becton Dickinson Company Ltd Tumor and tissue preservation reagent
US20250389636A1 (en) 2024-06-24 2025-12-25 Becton, Dickinson And Company Methods and systems for gain-independent data normalization in flow cytometry data and systems for same
US20260002864A1 (en) 2024-06-28 2026-01-01 Becton, Dickinson And Company Methods for determining positional flow stream velocity and systems for same
US20260009715A1 (en) 2024-07-03 2026-01-08 Becton, Dickinson And Company Method for flow cytometry quality scores and systems for same
US20260063530A1 (en) 2024-08-27 2026-03-05 Becton, Dickinson And Company Methods and compositions for flow cytometer calibration
US20260086018A1 (en) 2024-09-26 2026-03-26 Becton, Dickinson And Company Methods for detector gain modulation and systems for same
EP4726362A1 (en) 2024-10-09 2026-04-15 Becton Dickinson and Company Methods for monitoring flow rate of a flow stream and systems for same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110164246A1 (en) * 2008-06-13 2011-07-07 Embl Heidelberg Next generation flow cytometer sorter
WO2015143041A1 (en) * 2014-03-18 2015-09-24 The Regents Of The University Of California Parallel flow cytometer using radiofrequency mulitplexing

Family Cites Families (198)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6295A (en) 1849-04-10 Improvement in the manufacture of twine
US168A (en) 1837-04-17 Improvement in fire-arms
US3380584A (en) 1965-06-04 1968-04-30 Atomic Energy Commission Usa Particle separator
US3854050A (en) 1973-09-11 1974-12-10 Department Of Health Education High precision fluorometer for measuring enzymatic substrates in tissue
US3960449A (en) 1975-06-05 1976-06-01 The Board Of Trustees Of Leland Stanford Junior University Measurement of angular dependence of scattered light in a flowing stream
US4347935A (en) 1979-05-16 1982-09-07 The United States Of America As Represented By The United States Department Of Energy Method and apparatus for electrostatically sorting biological cells
US4667830A (en) 1981-06-15 1987-05-26 The Board Of Trustees Of The Leland Stanford Junior University Method and means for sorting individual particles into containers for culturing, cloning, analysis, or the like
US4545677A (en) * 1984-03-05 1985-10-08 Becton, Dickinson And Company Prismatic beam expander for light beam shaping in a flow cytometry apparatus
US4770992A (en) 1985-11-27 1988-09-13 Den Engh Gerrit J Van Detection of specific DNA sequences by flow cytometry
DE3610396A1 (de) 1986-03-27 1987-10-01 Wella Ag Mittel und verfahren zur oxidativen faerbung von haaren
US4704891A (en) 1986-08-29 1987-11-10 Becton, Dickinson And Company Method and materials for calibrating flow cytometers and other analysis instruments
US5245318A (en) 1987-07-24 1993-09-14 Canon Kabushiki Kaisha Particle analyzing apparatus having pressure control system
US5040890A (en) 1987-11-25 1991-08-20 Becton, Dickinson And Company Sheathed particle flow controlled by differential pressure
US5047321A (en) 1988-06-15 1991-09-10 Becton Dickinson & Co. Method for analysis of cellular components of a fluid
US5030002A (en) 1989-08-11 1991-07-09 Becton, Dickinson And Company Method and apparatus for sorting particles with a moving catcher tube
JPH0810306B2 (ja) 1990-01-26 1996-01-31 大日本スクリーン製造株式会社 光ビームの偏向制御方法
JPH0427866A (ja) * 1990-05-23 1992-01-30 Omron Corp 細胞選別装置
US5204884A (en) * 1991-03-18 1993-04-20 University Of Rochester System for high-speed measurement and sorting of particles
US5504337A (en) 1990-10-10 1996-04-02 Joseph R. Lakowicz Method and apparatus for performing phase fluorescence lifetime measurements in flow cytometry
WO1992013265A1 (en) 1991-01-24 1992-08-06 The University Of Maryland Method and apparatus for multi-dimensional phase fluorescence lifetime imaging
EP0515211A3 (en) 1991-05-23 1993-04-07 Becton Dickinson And Company Apparatus and method for phase resolved fluorescence lifetimes of independent and varying amplitude pulses
US5203339A (en) * 1991-06-28 1993-04-20 The Government Of The United States Of America As Represented By The Secretary Of The Department Health And Human Services Method and apparatus for imaging a physical parameter in turbid media using diffuse waves
ES2102518T3 (es) 1991-08-28 1997-08-01 Becton Dickinson Co Motor de atraccion por gravitacion para el agrupamiento autoadaptativo de corrientes de datos n-dimensionales.
US5309912A (en) 1991-11-08 1994-05-10 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Multidimensional imaging using a single point detector for a phase encoded modulated optical carrier
US5192870A (en) 1992-01-14 1993-03-09 International Business Machines Corporation Optical submicron aerosol particle detector
US5255257A (en) 1992-03-04 1993-10-19 Lasertape Systems, Inc. Frequency, phase and amplitude control apparatus and method for acousto-optic deflector optimization
US6057814A (en) 1993-05-24 2000-05-02 Display Science, Inc. Electrostatic video display drive circuitry and displays incorporating same
US5296911A (en) 1992-07-16 1994-03-22 Schiapparelli Biosystems, Inc. Optical test system for a chemical analyzer
US5270548A (en) * 1992-07-31 1993-12-14 The United States Of America As Represented By The United States Department Of Energy Phase-sensitive flow cytometer
US5293213A (en) 1992-08-12 1994-03-08 Klein Uwe K A Utilization of a modulated laser beam in heterodyne interferometry
JPH06186156A (ja) * 1992-10-21 1994-07-08 Toa Medical Electronics Co Ltd 粒子分析装置
US5464581A (en) 1993-08-02 1995-11-07 The Regents Of The University Of California Flow cytometer
US5483469A (en) 1993-08-02 1996-01-09 The Regents Of The University Of California Multiple sort flow cytometer
US5489977A (en) * 1993-08-11 1996-02-06 Texaco Inc. Photomeric means for monitoring solids and fluorescent material in waste water using a falling stream water sampler
US5700692A (en) 1994-09-27 1997-12-23 Becton Dickinson And Company Flow sorter with video-regulated droplet spacing
US5643796A (en) 1994-10-14 1997-07-01 University Of Washington System for sensing droplet formation time delay in a flow cytometer
US5602039A (en) 1994-10-14 1997-02-11 The University Of Washington Flow cytometer jet monitor system
US5608519A (en) * 1995-03-20 1997-03-04 Gourley; Paul L. Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells
US5620842A (en) 1995-03-29 1997-04-15 Becton Dickinson And Company Determination of the number of fluorescent molecules on calibration beads for flow cytometry
JPH0961858A (ja) 1995-08-28 1997-03-07 Minolta Co Ltd 音響光学光走査装置
US5968738A (en) * 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
US5968737A (en) 1996-11-12 1999-10-19 The University Of Mississippi Method of identifying inhibitors of glutathione S-transferase (GST) gene expression
JPH10148778A (ja) 1996-11-18 1998-06-02 Minolta Co Ltd マルチビーム発生装置
DE19721881C2 (de) 1997-05-26 1999-05-20 Bosch Gmbh Robert Interferometrische Meßvorrichtung
DE19721882C2 (de) 1997-05-26 1999-04-29 Bosch Gmbh Robert Interferometrische Meßvorrichtung
US6031852A (en) 1997-05-30 2000-02-29 The Regents Of The University Of California Rapid acoustooptic tuner and phase-shifter
US6016196A (en) 1997-06-17 2000-01-18 Massachusetts Institute Of Technology Multiple beam pair optical imaging
US6236454B1 (en) 1997-12-15 2001-05-22 Applied Materials, Inc. Multiple beam scanner for an inspection system
US6821740B2 (en) 1998-02-25 2004-11-23 Becton, Dickinson And Company Flow cytometric methods for the concurrent detection of discrete functional conformations of PRB in single cells
US6642018B1 (en) * 1998-03-27 2003-11-04 Oncosis Llc Method for inducing a response in one or more targeted cells
US6592822B1 (en) 1998-05-14 2003-07-15 Luminex Corporation Multi-analyte diagnostic system and computer implemented process for same
ES2253910T3 (es) * 1998-10-01 2006-06-01 2C A/S Aparatos y procedimiento para determinar la posicion de objetos marcados y para la inspeccion posterior de dichos objetos.
US6271924B1 (en) 1998-12-29 2001-08-07 Bryan Kok Ann Ngoi Noncontact acoustic optic scanning laser vibrometer for determining the difference between an object and a reference surface
GB2367616B (en) 1999-06-23 2004-06-16 Patrick Toomey Water detection and source identification methods for structures using electromagnetic radiation spectroscopy
US6396069B1 (en) 1999-06-25 2002-05-28 Macpherson David C. Topographer for real time ablation feedback having synthetic wavelength generators
US6372506B1 (en) 1999-07-02 2002-04-16 Becton, Dickinson And Company Apparatus and method for verifying drop delay in a flow cytometer
US6813017B1 (en) 1999-10-20 2004-11-02 Becton, Dickinson And Company Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer
US7300457B2 (en) 1999-11-19 2007-11-27 Advanced Bio Prosthetic Surfaces, Ltd. Self-supporting metallic implantable grafts, compliant implantable medical devices and methods of making same
US6295168B1 (en) 1999-12-15 2001-09-25 International Business Machines Corporation Refractive optical system that converts a laser beam to a collimated flat-top beam
US6809804B1 (en) 2000-05-11 2004-10-26 Becton, Dickinson And Company System and method for providing improved event reading and data processing capabilities in a flow cytometer
US7630063B2 (en) 2000-08-02 2009-12-08 Honeywell International Inc. Miniaturized cytometer for detecting multiple species in a sample
US6778263B2 (en) 2000-08-25 2004-08-17 Amnis Corporation Methods of calibrating an imaging system using calibration beads
JP2002296178A (ja) 2001-03-30 2002-10-09 Shimadzu Corp フローセル検出器
US6636623B2 (en) 2001-08-10 2003-10-21 Visiongate, Inc. Optical projection imaging system and method for automatically detecting cells with molecular marker compartmentalization associated with malignancy and disease
US6683314B2 (en) 2001-08-28 2004-01-27 Becton, Dickinson And Company Fluorescence detection instrument with reflective transfer legs for color decimation
FR2830629B1 (fr) 2001-10-04 2004-01-09 Commissariat Energie Atomique Procede et dispositif de prelevement d'une partie d'un faisceau lumineux, notamment pour appareil d'analyse de fluorescence
DE20216583U1 (de) 2001-12-20 2003-01-23 Leica Microsystems Heidelberg Gmbh, 68165 Mannheim Mikroskop und Durchflusszytometer
US6868347B2 (en) 2002-03-19 2005-03-15 The Regents Of The University Of California System for real time, non-invasive metrology of microfluidic chips
WO2003089901A2 (en) 2002-04-19 2003-10-30 Palsson Bernhard O Methods for preparing libraries of unique tags and related screening methods
US20060014212A1 (en) * 2002-05-10 2006-01-19 Epitome Biosystems, Inc. Proteome epitope tags and methods of use thereof in protein modification analysis
DE10225838B4 (de) 2002-06-11 2021-10-21 Leica Microsystems Cms Gmbh Verfahren zur Scanmikroskopie, Scanmikroskop und Vorrichtung zum Codieren eines Beleuchtungslichtstrahles
US7201875B2 (en) 2002-09-27 2007-04-10 Becton Dickinson And Company Fixed mounted sorting cuvette with user replaceable nozzle
EP1616286A1 (en) * 2003-04-02 2006-01-18 Amersham Biosciences UK Limited Method of, and computer software for, classification of cells into subpopulations
JP4533382B2 (ja) 2003-08-28 2010-09-01 セルラ・インコーポレイテッド マイクロ流体分析およびソーティング用の一体化された構造物
US7803624B2 (en) 2003-09-30 2010-09-28 Cytyc Corporation Automated cytological sample classification
US7103402B2 (en) 2003-10-02 2006-09-05 Ut-Battelle, Llc Advanced synchronous luminescence imaging for chemical and medical diagnostics
US7590991B2 (en) 2003-10-09 2009-09-15 Terayon Communication Systems, Inc. Method and apparatus for determining channel to which a TV or VCR is tuned
JP5567246B2 (ja) 2003-10-27 2014-08-06 ザ ジェネラル ホスピタル コーポレイション 周波数ドメイン干渉測定を利用して光学撮像を実行する方法および装置
DE10357584B4 (de) 2003-12-08 2006-06-14 Leica Microsystems Cms Gmbh Verfahren zum Trennen unterschiedlicher Emissionswellenlängen in einem Scanmikroskop
JP4304120B2 (ja) 2004-04-30 2009-07-29 ベイバイオサイエンス株式会社 生物学的粒子をソーティングする装置及び方法
US7133188B2 (en) 2004-06-07 2006-11-07 Electro Scientific Industries, Inc. AOM modulation techniques employing an upstream Bragg adjustment device
US20050287680A1 (en) 2004-06-25 2005-12-29 Srivatsa Venkatasubbarao Multianalyte assay method
EP1828748A1 (en) * 2004-12-17 2007-09-05 Luminex Corporation Systems, illumination subsystems, and methods for increasing fluorescence emitted by a fluorophore
US20060149479A1 (en) 2004-12-30 2006-07-06 Art, Advanced Research Technologies Inc. Method for improving fluorescence image contrast
CN101223560B (zh) * 2005-07-15 2012-12-12 百维吉伦特系统有限公司 病原体和颗粒的检测器系统和方法
US8060316B2 (en) 2005-08-02 2011-11-15 Luminex Corporation Methods, data structures, and systems for classifying microparticles
JP4756948B2 (ja) * 2005-08-08 2011-08-24 ベイバイオサイエンス株式会社 フローサイトメータおよびフローサイトメトリ方法
AU2006299659A1 (en) 2005-09-29 2007-04-12 General Hospital Corporation Method and apparatus for method for viewing and analyzing of one or more biological samples with progressively increasing resolutions
US7889348B2 (en) 2005-10-14 2011-02-15 The General Hospital Corporation Arrangements and methods for facilitating photoluminescence imaging
GB0525072D0 (en) 2005-12-09 2006-01-18 Enigma Diagnostics Ltd Fluorescence-based detection methods and apparatus
EP2899561B1 (en) 2006-02-17 2021-04-28 Regents of the University of Minnesota MRI method for generating a map of the transmit RF field for each coil of a RF coil array
US20090323061A1 (en) 2006-02-28 2009-12-31 Lukas Novotny Multi-color hetereodyne interferometric apparatus and method for sizing nanoparticles
JP2007285999A (ja) * 2006-04-20 2007-11-01 Furukawa Electric Co Ltd:The 光測定装置
WO2007133465A2 (en) * 2006-05-12 2007-11-22 Immunivest Corporation A laser illumination system in fluorescent microscopy
EP1862838B1 (en) 2006-05-29 2009-08-19 Olympus Corporation Laser scanning microscope and microscopic observing method
JP5133600B2 (ja) 2006-05-29 2013-01-30 オリンパス株式会社 レーザ走査型顕微鏡および顕微鏡観察方法
JP2008007652A (ja) 2006-06-29 2008-01-17 Fujifilm Corp アゾ色素、感熱転写記録用インクシート、感熱転写記録方法、カラートナー、インクジェット用インクおよびカラーフィルタ
US20100032584A1 (en) * 2006-08-18 2010-02-11 Macquarie University Tiime gated fluorescent flow cytometer
US7697885B2 (en) 2006-09-15 2010-04-13 Aeroflex High Speed Test Solutions, Inc. Multi-band jammer
US7804594B2 (en) 2006-12-29 2010-09-28 Abbott Laboratories, Inc. Method and apparatus for rapidly counting and identifying biological particles in a flow stream
US7400457B1 (en) 2007-01-04 2008-07-15 Stockeryale Canada Inc. Rectangular flat-top beam shaper
US7738094B2 (en) 2007-01-26 2010-06-15 Becton, Dickinson And Company Method, system, and compositions for cell counting and analysis
US8101426B2 (en) * 2007-03-02 2012-01-24 Icyt Mission Technology, Inc. System and method for the measurement of multiple fluorescence emissions in a flow cytometry system
US8290625B2 (en) 2007-04-04 2012-10-16 Beckman Coulter, Inc. Flow cytometer sorter
US7982944B2 (en) 2007-05-04 2011-07-19 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Method and apparatus for optical frequency comb generation using a monolithic micro-resonator
JP5619344B2 (ja) 2007-05-04 2014-11-05 マツクス−プランク−ゲゼルシャフト ツール フエルデルング デル ヴイツセンシャフテン エー フアウ モノリシックマイクロ共振器を使用した光周波数コム発生のための装置と方法
WO2009005579A1 (en) 2007-06-14 2009-01-08 The General Hospital Corporation High affinity fluorochrome binding peptides
EP2171098B1 (en) 2007-06-29 2018-03-28 Becton, Dickinson and Company Methods for extraction and purification of components of biological samples
US8848199B2 (en) 2007-07-10 2014-09-30 Massachusetts Institute Of Technology Tomographic phase microscopy
JP4509163B2 (ja) 2007-10-26 2010-07-21 ソニー株式会社 微小粒子の測定方法
JP4389991B2 (ja) * 2007-10-26 2009-12-24 ソニー株式会社 微小粒子の光学的測定方法及び光学的測定装置
US8159670B2 (en) 2007-11-05 2012-04-17 Abbott Laboratories Method and apparatus for rapidly counting and identifying biological particles in a flow stream
US9451884B2 (en) * 2007-12-13 2016-09-27 Board Of Trustees Of The University Of Arkansas Device and method for in vivo detection of clots within circulatory vessels
GB0800333D0 (en) 2008-01-09 2008-02-20 Ucl Business Plc Beam deflection apparatus and methods
US20090219607A1 (en) 2008-01-17 2009-09-03 Baylor College Of Medicine Method and apparatus for enhanced resolution microscopy of living biological nanostructures
US7920261B2 (en) 2008-02-11 2011-04-05 Massachusetts Institute Of Technology Method and apparatus for detecting and discriminating particles in a fluid
WO2009133734A1 (ja) 2008-05-02 2009-11-05 オリンパス株式会社 光学的検査装置、電磁波検出方法、電磁波検出装置、生態観察方法、顕微鏡、および、内視鏡並びに光断層画像生成装置
US8140300B2 (en) 2008-05-15 2012-03-20 Becton, Dickinson And Company High throughput flow cytometer operation with data quality assessment and control
US8184279B2 (en) 2008-06-16 2012-05-22 The Regents Of The University Of Colorado, A Body Corporate Fourier domain sensing
WO2010032452A1 (ja) * 2008-09-19 2010-03-25 三井造船株式会社 強度変調したレーザ光による蛍光検出装置および蛍光検出方法
EP2340429B1 (en) 2008-10-14 2022-08-24 Tissuevision, Inc. Devices and methods for direct sampling analog time-resolved detection
US8440952B2 (en) 2008-11-18 2013-05-14 The Regents Of The University Of California Methods for optical amplified imaging using a two-dimensional spectral brush
AU2009322907B2 (en) 2008-12-05 2016-05-05 The Ohio State University Research Foundation MicroRNA-based methods and compositions for the diagnosis and treatment of ovarian cancer
US8233146B2 (en) 2009-01-13 2012-07-31 Becton, Dickinson And Company Cuvette for flow-type particle analyzer
KR101224976B1 (ko) * 2009-01-22 2013-01-22 미쯔이 죠센 가부시키가이샤 형광 검출 장치 및 형광 검출 방법
US20110278471A1 (en) * 2009-01-23 2011-11-17 Mitsui Engineering & Shipbuilding Co., Ltd Fluorescence detecting device and fluorescence detecting method
JP4564566B2 (ja) * 2009-02-13 2010-10-20 三井造船株式会社 蛍光検出装置及び蛍光検出方法
JP4620786B2 (ja) * 2009-02-17 2011-01-26 三井造船株式会社 蛍光検出方法、蛍光検出装置及びプログラム
JP2012525146A (ja) * 2009-04-28 2012-10-22 プレジデント アンド フェロウズ オブ ハーバード カレッジ 細胞透過のための過剰に荷電されたタンパク質
TWI594828B (zh) * 2009-05-28 2017-08-11 伊雷克托科學工業股份有限公司 應用於雷射處理工件中的特徵的聲光偏轉器及相關雷射處理方法
EP2264168B1 (de) 2009-06-18 2014-12-17 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren
JP5612278B2 (ja) 2009-06-23 2014-10-22 パナソニック株式会社 三次元形状造形物の製造方法およびその製造装置
EP2267430A1 (de) * 2009-06-24 2010-12-29 Masterrind GmbH Vorrichtung und Verfahren zur Selektion von Partikeln
WO2011023593A1 (en) 2009-08-24 2011-03-03 INSERM (Institut National de la Santé et de la Recherche Médicale) Method of and apparatus for imaging a cellular sample
JP5534214B2 (ja) * 2009-10-05 2014-06-25 ベイバイオサイエンス株式会社 フローサイトメータおよびフローサイトメトリ方法
GB0919854D0 (en) * 2009-11-12 2009-12-30 Stfc Science & Technology Detecting species in a dilute medium
JP5649828B2 (ja) 2010-02-03 2015-01-07 オリンパス株式会社 レーザ顕微鏡装置
JP2011163787A (ja) * 2010-02-05 2011-08-25 Sony Corp 微小粒子分析装置及び微小粒子分析方法
US9057676B2 (en) 2010-02-05 2015-06-16 Cytonome/St, Llc Multiple flow channel particle analysis system
JP5551477B2 (ja) 2010-03-15 2014-07-16 オリンパス株式会社 光源装置およびレーザ走査型顕微鏡装置
JP5437148B2 (ja) 2010-04-23 2014-03-12 ベイバイオサイエンス株式会社 フローサイトメータおよびセルソータ
US9101573B2 (en) 2010-05-04 2015-08-11 Virginia Tech Intellectual Properties, Inc. Lanthionine synthetase component C-like proteins as molecular targets for preventing and treating diseases and disorders
JP2012008055A (ja) 2010-06-25 2012-01-12 Olympus Corp 画像解析方法および画像解析装置
US8384045B2 (en) 2010-07-01 2013-02-26 Sony Corporation Minute particle analyzing device and method
US8574838B2 (en) 2010-07-14 2013-11-05 University Of Medicine And Dentistry Of New Jersey Methods and kits for miRNA isolation and quantitation
US8528427B2 (en) 2010-10-29 2013-09-10 Becton, Dickinson And Company Dual feedback vacuum fluidics for a flow-type particle analyzer
EP2637015A4 (en) * 2010-11-01 2016-09-07 Kanagawa Kagaku Gijutsu Akad cell analyzer
SG10201912164UA (en) 2010-11-16 2020-02-27 1087 Systems Inc System for identifying and sorting living cells
DE102010044013A1 (de) 2010-11-16 2012-05-16 Carl Zeiss Microimaging Gmbh Tiefenauflösungsgesteigerte Mikroskopie
US9066657B2 (en) 2010-11-23 2015-06-30 General Electric Company Methods and systems of optical imaging for target detection in a scattering medium
US10481069B2 (en) 2011-01-03 2019-11-19 Cytonome/St, Llc Method and apparatus for monitoring and optimizing microfluidic particle sorting
US8705031B2 (en) * 2011-02-04 2014-04-22 Cytonome/St, Llc Particle sorting apparatus and method
DE112012001802T5 (de) 2011-03-18 2014-03-13 Riken Nichtlineares optisches Mikroskop und Nichtlineare optische Mikroskopie
US8990047B2 (en) 2011-03-21 2015-03-24 Becton, Dickinson And Company Neighborhood thresholding in mixed model density gating
JP5620319B2 (ja) 2011-03-23 2014-11-05 オリンパス株式会社 顕微鏡
EP2702132B1 (en) 2011-04-29 2018-11-21 Becton Dickinson and Company Multi-way sorter system and method
CN103703635B (zh) 2011-05-16 2017-07-25 Oe电波公司 基于非线性光学谐振器的三重振荡器装置
EP2713878B1 (en) 2011-05-26 2021-07-07 The General Hospital Corporation Optical thromboelastography system and method for evaluation of blood coagulation metrics
CA2842377C (en) 2011-07-19 2019-08-27 Ovizio Imaging Systems N.V. A method and system for detecting and/or classifying cancerous cells in a cell sample
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
WO2013049623A1 (en) 2011-09-30 2013-04-04 Brian David Warner Fluid exchange methods and devices
JP5870851B2 (ja) 2012-05-29 2016-03-01 ソニー株式会社 情報処理装置、情報処理方法、及びプログラム
JP2014002470A (ja) 2012-06-15 2014-01-09 Ricoh Co Ltd 処理装置、処理システム、出力方法およびプログラム
US9168568B2 (en) * 2012-08-01 2015-10-27 Owl biomedical, Inc. Particle manipulation system with cytometric confirmation
WO2014031985A1 (en) 2012-08-24 2014-02-27 The Trustees Of Dartmouth College Method and apparatus for magnetic susceptibility tomography, magnetoencephalography, and taggant or contrast agent detection
US8723104B2 (en) 2012-09-13 2014-05-13 City University Of Hong Kong Methods and means for manipulating particles
CN105008895B (zh) 2012-10-15 2019-02-15 纳诺赛莱克特生物医药股份有限公司 颗粒分选的系统、设备和方法
EP2943778B1 (en) 2013-01-09 2020-10-28 The Regents of The University of California Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation
WO2014113614A1 (en) 2013-01-16 2014-07-24 The Regents Of The University Of California Label free molecular detection methods, systems and devices
US10190963B2 (en) 2013-02-01 2019-01-29 Becton, Dickinson And Company Methods and systems for assessing sample behavior in a flow cytometer
WO2014152048A2 (en) 2013-03-14 2014-09-25 Cytonome/St, Llc Assemblies and methods for reducing optical crosstalk in particle processing systems
EP2972206B1 (en) 2013-03-14 2024-02-21 Cytonome/ST, LLC Operatorless particle processing systems and methods
GB201305317D0 (en) 2013-03-22 2013-05-08 Iles Raymond K Prenatal screening for fetal abnormalities and disorders of pregnancy
CN105143851B (zh) 2013-04-12 2019-03-01 贝克顿·迪金森公司 用于细胞分选的自动化设置
US9372143B2 (en) * 2013-05-15 2016-06-21 Captl Llc Scanning image flow cytometer
JP6136843B2 (ja) * 2013-10-17 2017-05-31 ソニー株式会社 粒子分取装置、粒子分取方法及びプログラム
JP6257312B2 (ja) 2013-12-24 2018-01-10 オリンパス株式会社 顕微鏡システム
US9538926B2 (en) 2013-12-26 2017-01-10 Fundacio Institut De Ciencies Fotoniques Speckle contrast optical tomography
CN103762487B (zh) 2014-01-04 2016-05-25 天津奇谱光电技术有限公司 一种具有双输出光束的可调谐激光器
JP6214402B2 (ja) 2014-01-07 2017-10-18 オリンパス株式会社 光刺激装置および顕微鏡システム
US9528925B2 (en) * 2014-02-14 2016-12-27 Palo Alto Research Center Incorporated Spatial modulation of light to determine object position
US9207066B2 (en) * 2014-02-14 2015-12-08 Palo Alto Research Center Incorporated Spatial modulation of light to determine dimensional characteristics of objects in a flow path
US10451482B2 (en) * 2014-02-14 2019-10-22 Palo Alto Research Center Incorporated Determination of color characteristics of objects using spatially modulated light
US20170052106A1 (en) 2014-04-28 2017-02-23 The Broad Institute, Inc. Method for label-free image cytometry
WO2016054293A1 (en) 2014-09-30 2016-04-07 The Regents Of The University Of California Imaging flow cytometer using spatial-temporal transformation
CN107111205B (zh) 2014-11-12 2020-12-15 奥宝科技有限公司 具有多个输出光束的声光偏光器
WO2017053592A1 (en) 2015-09-23 2017-03-30 The Regents Of The University Of California Deep learning in label-free cell classification and machine vision extraction of particles
CN108474728B (zh) 2015-10-13 2022-04-26 贝克顿·迪金森公司 多模态荧光成像流式细胞术系统
US10337997B2 (en) 2015-10-15 2019-07-02 Woods Hole Oceanographic Institution System for rapid assessment of water quality and harmful algal bloom toxins
JP7033082B2 (ja) 2016-02-05 2022-03-09 ナノビュー バイオサイエンシズ インコーポレイテッド 表面マーカーを有するエキソソームの検出
JP7130562B2 (ja) * 2016-03-17 2022-09-05 ベクトン・ディキンソン・アンド・カンパニー ハイスループット蛍光フローサイトメーターを用いる細胞選別
WO2017201540A1 (en) 2016-05-20 2017-11-23 Techcyte, Inc. Machine learning classification of particles or substances in digital microscopy images
FI3469338T3 (fi) 2016-06-10 2025-05-06 Univ California Kuvapohjaisia solunlajittelujärjestelmiä ja -menetelmiä
CN109477785B (zh) 2016-09-13 2022-09-27 贝克顿·迪金森公司 具有光学均衡的流式细胞仪
EP3695204A4 (en) 2017-10-09 2021-07-07 TSI Incorporated PARTICLE COUNTER COMPONENT CALIBRATION
WO2019199499A1 (en) 2018-04-13 2019-10-17 University Of Washington Methods and apparatus for single biological nanoparticle analysis
EP3785012A4 (en) 2018-04-26 2022-02-09 Becton, Dickinson and Company CHARACTERIZATION AND SORTING FOR PARTICLE ANALYZERS
JP7466563B2 (ja) 2019-03-29 2024-04-15 ベクトン・ディキンソン・アンド・カンパニー 粒子の区別に使用するためのパラメータ

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110164246A1 (en) * 2008-06-13 2011-07-07 Embl Heidelberg Next generation flow cytometer sorter
WO2015143041A1 (en) * 2014-03-18 2015-09-24 The Regents Of The University Of California Parallel flow cytometer using radiofrequency mulitplexing

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HOUSTON, J.P., et al., ‘Digital Analysis and Sorting of Fluorescence Lifetime By Flow Cytometry’, Journal of Quantitative Cell Science Cytometry part A., vol.77A, issue.9 23 August 2010, pp.861-872. *
JENKINS, P. et al., ‘Toward the measurement of multiple fluorescence lifetimes in flow cytometry: maximizing multi-harmonic content from cells and microspheres’, Journal of Biophotonics, Wiley, vol.8, iss.11-12, 26 Feb 2015 pp 908-917 *

Also Published As

Publication number Publication date
US20230076378A1 (en) 2023-03-09
JP7130562B2 (ja) 2022-09-05
US20250155356A1 (en) 2025-05-15
KR20230003444A (ko) 2023-01-05
US12313521B1 (en) 2025-05-27
US11105728B2 (en) 2021-08-31
JP2022095825A (ja) 2022-06-28
US20210356381A1 (en) 2021-11-18
JP7502361B2 (ja) 2024-06-18
US10620111B2 (en) 2020-04-14
WO2017161247A1 (en) 2017-09-21
US12078587B2 (en) 2024-09-03
EP4545936A2 (en) 2025-04-30
KR102527830B1 (ko) 2023-05-02
KR20180129832A (ko) 2018-12-05
EP3430376A1 (en) 2019-01-23
BR112018068866B1 (pt) 2022-11-29
EP3430376B1 (en) 2024-10-23
US20200209140A1 (en) 2020-07-02
EP4545936A3 (en) 2025-07-16
US20170268981A1 (en) 2017-09-21
EP3430376B8 (en) 2024-11-27
AU2017234815A1 (en) 2018-10-04
KR102641469B1 (ko) 2024-02-28
CN109564151B (zh) 2023-01-24
US10324019B2 (en) 2019-06-18
US20190234863A1 (en) 2019-08-01
US20240410813A1 (en) 2024-12-12
KR20220002706A (ko) 2022-01-06
CA3018065A1 (en) 2017-09-21
BR112018068866A2 (pt) 2019-01-22
JP2019513236A (ja) 2019-05-23
CN109564151A (zh) 2019-04-02
US11774343B2 (en) 2023-10-03
ZA201806386B (en) 2020-02-26
JP2024119909A (ja) 2024-09-03

Similar Documents

Publication Publication Date Title
US12313521B1 (en) Cell sorting using a high throughput fluorescence flow cytometer
US12038372B2 (en) Multi-modal fluorescence imaging flow cytometry system
US12436087B2 (en) Spectral unmixing of fluorescence imaging using radiofrequency-multiplexed excitation data

Legal Events

Date Code Title Description
PC1 Assignment before grant (sect. 113)

Owner name: BECTON, DICKINSON AND COMPANY

Free format text: FORMER APPLICANT(S): BD BIOSCIENCES

FGA Letters patent sealed or granted (standard patent)