AU2011305445B2 - Direct capture, amplification and sequencing of target DNA using immobilized primers - Google Patents

Direct capture, amplification and sequencing of target DNA using immobilized primers Download PDF

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Publication number
AU2011305445B2
AU2011305445B2 AU2011305445A AU2011305445A AU2011305445B2 AU 2011305445 B2 AU2011305445 B2 AU 2011305445B2 AU 2011305445 A AU2011305445 A AU 2011305445A AU 2011305445 A AU2011305445 A AU 2011305445A AU 2011305445 B2 AU2011305445 B2 AU 2011305445B2
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sequence
adaptor
seq
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primer
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Jason Buenrostro
Hanlee P. Ji
Samuel Myllykangas
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Leland Stanford Junior University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2011305445A 2010-09-24 2011-09-21 Direct capture, amplification and sequencing of target DNA using immobilized primers Active AU2011305445B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US38639010P 2010-09-24 2010-09-24
US61/386,390 2010-09-24
US201161485062P 2011-05-11 2011-05-11
US61/485,062 2011-05-11
PCT/US2011/052645 WO2012040387A1 (en) 2010-09-24 2011-09-21 Direct capture, amplification and sequencing of target dna using immobilized primers

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AU2011305445A1 AU2011305445A1 (en) 2013-04-04
AU2011305445B2 true AU2011305445B2 (en) 2017-03-16

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US (3) US9309556B2 (enExample)
EP (2) EP2619329B1 (enExample)
JP (1) JP5986572B2 (enExample)
KR (1) KR20130113447A (enExample)
CN (1) CN103228798B (enExample)
AU (1) AU2011305445B2 (enExample)
CA (1) CA2810931C (enExample)
IL (1) IL225109A (enExample)
IN (1) IN2013MN00522A (enExample)
MX (1) MX346956B (enExample)
NZ (1) NZ608313A (enExample)
RU (1) RU2565550C2 (enExample)
WO (1) WO2012040387A1 (enExample)

Families Citing this family (153)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
EP3216874A1 (en) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Methods for stratifying and annotating cancer drug treatment options
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
CA2760439A1 (en) 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
DK2669387T3 (en) * 2009-08-25 2016-09-19 Illumina Inc Methods of selection and amplification of polynucleotides
RS54482B1 (sr) 2010-04-05 2016-06-30 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
IN2013MN00522A (enExample) 2010-09-24 2015-05-29 Univ Leland Stanford Junior
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
MX344363B (es) 2010-10-04 2016-12-14 Genapsys Inc * Sistemas y métodos para reacciones biológicas paralelas reutilizables automatizadas.
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
EP2768983A4 (en) 2011-10-17 2015-06-03 Good Start Genetics Inc METHODS OF IDENTIFYING MUTATIONS ASSOCIATED WITH DISEASES
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
WO2013082619A1 (en) 2011-12-01 2013-06-06 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
CA2862552A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
EP3495503A1 (en) 2012-03-05 2019-06-12 President and Fellows of Harvard College Systems and methods for epigenetic sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
CA2873176C (en) 2012-05-10 2024-02-27 The General Hospital Corporation Methods for determining a nucleotide sequence
SG11201408478QA (en) 2012-06-18 2015-02-27 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US20140024541A1 (en) * 2012-07-17 2014-01-23 Counsyl, Inc. Methods and compositions for high-throughput sequencing
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
JP6285929B2 (ja) * 2012-07-17 2018-02-28 カウンシル，インコーポレーテッド 遺伝的変異を検出するためのシステムおよび方法
CN103571822B (zh) * 2012-07-20 2016-03-30 中国科学院植物研究所 一种用于新一代测序分析的多重目的dna片段富集方法
KR102393608B1 (ko) * 2012-09-04 2022-05-03 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
EP2909337B1 (en) 2012-10-17 2019-01-09 Spatial Transcriptomics AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
CN111254500B (zh) 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
HK1217971A1 (zh) * 2013-02-21 2017-01-27 Toma Biosciences Inc 用於核酸分析的方法、組合物和試劑盒
EP2971159B1 (en) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Methods for analyzing nucleic acids
CN105051214B (zh) 2013-03-15 2018-12-28 吉纳普赛斯股份有限公司 用于生物分析的系统和方法
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
US8847799B1 (en) 2013-06-03 2014-09-30 Good Start Genetics, Inc. Methods and systems for storing sequence read data
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
US9898575B2 (en) 2013-08-21 2018-02-20 Seven Bridges Genomics Inc. Methods and systems for aligning sequences
US9116866B2 (en) 2013-08-21 2015-08-25 Seven Bridges Genomics Inc. Methods and systems for detecting sequence variants
US11049587B2 (en) 2013-10-18 2021-06-29 Seven Bridges Genomics Inc. Methods and systems for aligning sequences in the presence of repeating elements
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
CN105793689B (zh) 2013-10-18 2020-04-17 七桥基因公司 用于将遗传样本基因分型的方法和系统
CN105849279B (zh) 2013-10-18 2020-02-18 七桥基因公司 用于识别疾病诱导的突变的方法和系统
WO2015058095A1 (en) 2013-10-18 2015-04-23 Seven Bridges Genomics Inc. Methods and systems for quantifying sequence alignment
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
US9092402B2 (en) 2013-10-21 2015-07-28 Seven Bridges Genomics Inc. Systems and methods for using paired-end data in directed acyclic structure
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015081229A2 (en) * 2013-11-26 2015-06-04 Lc Sciences Llc Selective amplification of nucleic acid sequences
US10125393B2 (en) 2013-12-11 2018-11-13 Genapsys, Inc. Systems and methods for biological analysis and computation
CN105793438B (zh) * 2013-12-15 2020-02-11 中央研究院 未知序列的双股线性核酸的全长扩增方法
US9677132B2 (en) * 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
WO2015108663A1 (en) * 2014-01-16 2015-07-23 Illumina, Inc. Amplicon preparation and sequencing on solid supports
US9944924B2 (en) 2014-01-16 2018-04-17 Illumina, Inc. Polynucleotide modification on solid support
US10450597B2 (en) 2014-01-27 2019-10-22 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
EP3556864B1 (en) * 2014-04-18 2020-12-09 Genapsys, Inc. Methods and systems for nucleic acid amplification
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US11053548B2 (en) 2014-05-12 2021-07-06 Good Start Genetics, Inc. Methods for detecting aneuploidy
SG11201610168YA (en) * 2014-05-16 2017-01-27 Illumina Inc Nucleic acid synthesis techniques
US20150361422A1 (en) * 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
KR102425438B1 (ko) * 2014-06-23 2022-07-27 더 제너럴 하스피탈 코포레이션 서열결정에 의해 평가된 DSB의 게놈 전체에 걸친 비편향된 확인 (GUIDE-Seq)
US20160048608A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
EP3224595A4 (en) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
US9558321B2 (en) 2014-10-14 2017-01-31 Seven Bridges Genomics Inc. Systems and methods for smart tools in sequence pipelines
WO2016112073A1 (en) 2015-01-06 2016-07-14 Good Start Genetics, Inc. Screening for structural variants
EP4119677B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US11746367B2 (en) 2015-04-17 2023-09-05 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US10275567B2 (en) 2015-05-22 2019-04-30 Seven Bridges Genomics Inc. Systems and methods for haplotyping
EP3859013A1 (en) * 2015-06-02 2021-08-04 BioFire Defense, LLC Sample to sequence
RU2604198C1 (ru) * 2015-06-22 2016-12-10 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Способ получения гетерогенного набора одноцепочечных фрагментов днк для мультиплексного генетического анализа
DK3329012T3 (da) * 2015-07-27 2021-10-11 Illumina Inc Rumlig kortlægning af nukleinsyresekvensinformation
US10793895B2 (en) 2015-08-24 2020-10-06 Seven Bridges Genomics Inc. Systems and methods for epigenetic analysis
US10584380B2 (en) 2015-09-01 2020-03-10 Seven Bridges Genomics Inc. Systems and methods for mitochondrial analysis
US10724110B2 (en) 2015-09-01 2020-07-28 Seven Bridges Genomics Inc. Systems and methods for analyzing viral nucleic acids
WO2017044880A1 (en) * 2015-09-09 2017-03-16 uBiome, Inc. Method and system for microbiome-derived diagnostics and therapeutics infectious disease and other health conditions associated with antibiotic usage
KR102753113B1 (ko) 2015-09-18 2025-01-09 바나디스 다이아그노스틱스 Dna 샘플의 분석을 위한 프로브 세트 및 이의 사용 방법
US11347704B2 (en) 2015-10-16 2022-05-31 Seven Bridges Genomics Inc. Biological graph or sequence serialization
KR101651817B1 (ko) * 2015-10-28 2016-08-29 대한민국 Ngs 라이브러리 제작용 프라이머 세트 및 이를 이용한 ngs 라이브러리 제작방법 및 키트
AU2016353133B2 (en) 2015-11-11 2022-12-08 Resolution Bioscience, Inc. High efficiency construction of dna libraries
US10364468B2 (en) 2016-01-13 2019-07-30 Seven Bridges Genomics Inc. Systems and methods for analyzing circulating tumor DNA
US10460829B2 (en) 2016-01-26 2019-10-29 Seven Bridges Genomics Inc. Systems and methods for encoding genetic variation for a population
US10262102B2 (en) 2016-02-24 2019-04-16 Seven Bridges Genomics Inc. Systems and methods for genotyping with graph reference
WO2017168329A1 (en) * 2016-03-28 2017-10-05 Boreal Genomics, Inc. Droplet-based linked-fragment sequencing
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
US12416047B2 (en) 2016-05-06 2025-09-16 Myriad Women's Health, Inc. Noninvasive prenatal diagnostic methods
WO2017214557A1 (en) 2016-06-10 2017-12-14 Counsyl, Inc. Nucleic acid sequencing adapters and uses thereof
WO2018017884A1 (en) 2016-07-20 2018-01-25 Genapsys, Inc. Systems and methods for nucleic acid sequencing
EP4435116A3 (en) 2016-08-05 2024-12-18 Bio-Rad Laboratories, Inc. Second strand direct
AU2017315769B2 (en) 2016-08-25 2024-02-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
US11250931B2 (en) 2016-09-01 2022-02-15 Seven Bridges Genomics Inc. Systems and methods for detecting recombination
US10683531B2 (en) 2016-09-15 2020-06-16 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free DNA
EP3933039A1 (en) 2016-09-15 2022-01-05 ArcherDX, LLC Methods of nucleic acid sample preparation
CA3037366A1 (en) 2016-09-29 2018-04-05 Myriad Women's Health, Inc. Noninvasive prenatal screening using dynamic iterative depth optimization
EP3551769A4 (en) 2016-12-09 2020-10-28 Boreal Genomics, Inc. LINKED LIGATURE
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
EP3571616B1 (en) 2017-01-18 2021-05-19 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
WO2018144216A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
US10752946B2 (en) 2017-01-31 2020-08-25 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
US10787699B2 (en) * 2017-02-08 2020-09-29 Microsoft Technology Licensing, Llc Generating pluralities of primer and payload designs for retrieval of stored nucleotides
WO2018161019A1 (en) * 2017-03-03 2018-09-07 Counsyl, Inc. Methods for optimizing direct targeted sequencing
WO2018175907A1 (en) 2017-03-24 2018-09-27 Counsyl, Inc. Copy number variant caller
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
SG11202002516WA (en) 2017-09-21 2020-04-29 Genapsys Inc Systems and methods for nucleic acid sequencing
US11414687B2 (en) 2017-10-04 2022-08-16 Centrillion Technology Holdings Corporation Method and system for enzymatic synthesis of oligonucleotides
US11725228B2 (en) 2017-10-11 2023-08-15 The General Hospital Corporation Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN111788316B (zh) * 2018-02-07 2024-11-29 帝肯基因组学公司 库制备
US11898203B2 (en) 2018-04-17 2024-02-13 The General Hospital Corporation Highly sensitive in vitro assays to define substrate preferences and sites of nucleic-acid binding, modifying, and cleaving agents
US12497713B2 (en) 2018-05-08 2025-12-16 Mgi Tech Co., Ltd. Single tube bead-based DNA co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly
WO2020018824A1 (en) 2018-07-19 2020-01-23 Ultima Genomics, Inc. Nucleic acid clonal amplification and sequencing methods, systems, and kits
US12098419B2 (en) 2018-08-23 2024-09-24 Ncan Genomics, Inc. Linked target capture and ligation
US20210340611A1 (en) * 2018-10-09 2021-11-04 Academia Sinica Digital polymerase chain reaction method for detecting nucleic acids in samples
US12385083B2 (en) 2018-12-10 2025-08-12 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US12247198B2 (en) 2018-12-14 2025-03-11 Bgi Shenzhen Nucleic acid synthesis and purification device, use thereof, and nucleic acid synthesis and purification method
CA3103750A1 (en) * 2018-12-17 2020-06-25 Illumina Cambridge Limited Primer oligonucleotide for sequencing
EP3884071B1 (en) 2019-01-03 2025-03-26 Ncan Genomics, Inc. Linked target capture
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN114144188B (zh) * 2019-05-21 2024-05-28 台湾地区"中央研究院" 放大及检测核糖核酸(rna)片段的方法
CN112029841B (zh) * 2019-06-03 2024-02-09 香港中文大学 定量端粒长度和基因组基序的方法
CN117535379A (zh) * 2019-08-09 2024-02-09 深圳市真迈生物科技有限公司 一种捕获核酸分子的方法及其应用
US11795495B1 (en) * 2019-10-02 2023-10-24 FOXO Labs Inc. Machine learned epigenetic status estimator
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
GB2612412B (en) * 2020-01-31 2025-10-10 Agilent Technologies Inc Systems and methods for targeted nucleic acid capture
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
CA3145539A1 (en) * 2020-02-26 2021-09-02 Illumina, Inc. Kits for genotyping
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4150126A4 (en) * 2020-05-12 2024-06-19 Singular Genomics Systems, Inc. NUCLEIC ACID AMPLIFICATION PROCESSES
CN111676276A (zh) * 2020-07-13 2020-09-18 湖北伯远合成生物科技有限公司 一种快速精准确定基因编辑突变情况的方法及其应用
WO2022015600A2 (en) 2020-07-13 2022-01-20 Singular Genomics Systems, Inc. Methods of sequencing complementary polynucleotides
CA3187366A1 (en) * 2020-07-31 2022-02-03 Arc Bio, Llc Systems, methods, and media for determining relative quality of oligonucleotide preparations
WO2022055969A1 (en) * 2020-09-08 2022-03-17 Seqwell, Inc. Sequencing oligonucleotides and methods of use thereof
US12499970B2 (en) 2020-09-30 2025-12-16 Myriad Women's Health, Inc. Deep learning based variant calling using machine learning
RU2748380C1 (ru) * 2020-11-10 2021-05-25 федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ) Набор олигонуклеотидных праймеров и способ генотипирования однонуклеотидного полиморфизма rs8065080 в гене TRPV1 человека
CN112837749B (zh) * 2021-02-01 2021-11-26 北京百奥纳芯生物科技有限公司 一种癌症筛查用基因芯片探针的优选方法
EP4294920A4 (en) * 2021-04-27 2025-01-22 Singular Genomics Systems, Inc. HIGH-DENSITY SEQUENCING AND MULTIPLEX PRIMING
WO2023003757A1 (en) 2021-07-19 2023-01-26 Illumina Software, Inc. Intensity extraction with interpolation and adaptation for base calling
EP4373967A4 (en) * 2021-07-20 2025-06-11 Freenome Holdings, Inc. Compositions and methods for improved resolution of 5-hydroxymethylated cytosine in nucleic acid sequencing
CN114621307B (zh) * 2022-04-12 2024-12-13 中国科学院苏州生物医学工程技术研究所 一种寡核苷酸空间坐标编码方法及其微流控装置
WO2025072683A1 (en) * 2023-09-29 2025-04-03 Illumina, Inc. Solid supports and methods for strand invasion-based amplification

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080286795A1 (en) * 1997-04-01 2008-11-20 Solexa Limited Method of nucleic acid amplification
US20090117621A1 (en) * 2005-07-20 2009-05-07 Jonathan Mark Boutell Methods of nucleic acid amplification and sequencing
US20090117573A1 (en) * 2007-09-14 2009-05-07 Affymetrix, Inc. Locus specific amplification using array probes
US20100137143A1 (en) * 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes

Family Cites Families (267)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3575220A (en) 1968-08-12 1971-04-20 Scientific Industries Apparatus for dispensing liquid sample
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4739044A (en) 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US6127155A (en) 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US6090591A (en) 1987-07-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
US5231191A (en) 1987-12-24 1993-07-27 Applied Biosystems, Inc. Rhodamine phosphoramidite compounds
US4997928A (en) 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
AU4128089A (en) 1988-09-15 1990-03-22 Rorer International (Overseas) Inc. Monoclonal antibodies specific to human epidermal growth factor receptor and therapeutic methods employing same
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US7223833B1 (en) 1991-05-24 2007-05-29 Isis Pharmaceuticals, Inc. Peptide nucleic acid conjugates
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
DE69329800D1 (de) 1992-04-27 2001-02-01 Dartmouth College Detektion von gensequenzen in biologischen flüssigkeiten
ATE208658T1 (de) 1993-07-28 2001-11-15 Pe Corp Ny Vorrichtung und verfahren zur nukleinsäurevervielfältigung
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CH686982A5 (fr) 1993-12-16 1996-08-15 Maurice Stroun Méthode pour le diagnostic de cancers.
US5539083A (en) 1994-02-23 1996-07-23 Isis Pharmaceuticals, Inc. Peptide nucleic acid combinatorial libraries and improved methods of synthesis
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5789206A (en) 1995-07-07 1998-08-04 Myriad Genetics, Inc. Method for ligating adaptors to nucleic acids which methods are useful for obtaining the ends of genes
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
NO954667D0 (no) 1995-11-17 1995-11-17 Dagfinn Oegreid Fremgangsmåte til deteksjon av Ki-ras mutasjoner
US6156504A (en) 1996-03-15 2000-12-05 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
CA2249717A1 (en) 1996-03-18 1997-09-25 Richard K. Wilkosz Target nucleic acid sequence amplification
US6759217B2 (en) 1996-03-26 2004-07-06 Oncomedx, Inc. Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
SE9602829D0 (sv) 1996-07-19 1996-07-19 Yngve Johnsson Förfarande och anordning för betalningsmedelshantering jämte betalningsmedel
US5998140A (en) 1996-07-31 1999-12-07 The Scripps Research Institute Complex formation between dsDNA and oligomer of cyclic heterocycles
US6482795B1 (en) 1997-01-30 2002-11-19 Myriad Genetics, Inc. Tumor suppressor designated TS10q23.3
US6262242B1 (en) 1997-01-30 2001-07-17 Board Of Regents, The University Of Texas System Tumor suppressor designated TS10Q23.3
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
JPH10341047A (ja) 1997-06-06 1998-12-22 Sony Corp 磁気トンネル素子
ATE487790T1 (de) 1997-07-07 2010-11-15 Medical Res Council In-vitro-sortierverfahren
US6008002A (en) 1997-09-29 1999-12-28 Bodey; Bela Immunomagnetic detection and isolation of cancer cells
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
IL137802A0 (en) 1998-02-12 2001-10-31 Immunivest Corp A method and kit for detecting rare cells in a mixed cell population
JPH11355695A (ja) 1998-06-10 1999-12-24 Sony Corp 映像信号処理装置
DE19833738A1 (de) 1998-07-27 2000-02-03 Michael Giesing Verfahren zur Isolierung von Krebszellen aus zellhaltigen Körperflüssigkeiten sowie Sets zur Durchführung dieses Verfahrens
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6086740A (en) 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US6864052B1 (en) * 1999-01-06 2005-03-08 Callida Genomics, Inc. Enhanced sequencing by hybridization using pools of probes
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US9534254B1 (en) 1999-02-02 2017-01-03 Abbott Molecular Inc. Patient stratification for cancer therapy based on genomic DNA microarray analysis
US6395481B1 (en) 1999-02-16 2002-05-28 Arch Development Corp. Methods for detection of promoter polymorphism in a UGT gene promoter
US6410231B1 (en) 1999-02-26 2002-06-25 Incyte Genomics, Inc. SNP detection
US7324926B2 (en) 1999-04-09 2008-01-29 Whitehead Institute For Biomedical Research Methods for predicting chemosensitivity or chemoresistance
US20010034023A1 (en) 1999-04-26 2001-10-25 Stanton Vincent P. Gene sequence variations with utility in determining the treatment of disease, in genes relating to drug processing
US6492161B1 (en) 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
US6300070B1 (en) * 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
ATE503843T1 (de) 1999-09-01 2011-04-15 Whitehead Biomedical Inst Gesamt-chromosom analyse von protein-dns wechselwirkungen
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7205105B2 (en) 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
WO2001053460A1 (en) 2000-01-21 2001-07-26 Variagenics, Inc. Identification of genetic components of drug response
WO2001055720A2 (en) 2000-01-26 2001-08-02 Ventana Medical Systems, Inc. A system for developing assays for personalized medicine
AU2001241939A1 (en) 2000-02-28 2001-09-12 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
EP1975256B1 (en) 2000-03-01 2012-07-11 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
US20020120409A1 (en) 2000-05-19 2002-08-29 Affymetrix, Inc. Methods for gene expression analysis
WO2001096382A2 (en) 2000-06-15 2001-12-20 Prokaria Ehf. Thermostable cellulase
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
EP2299256A3 (en) 2000-09-15 2012-10-10 California Institute Of Technology Microfabricated crossflow devices and methods
JP2004509629A (ja) 2000-09-19 2004-04-02 ホワイトヘッド　インスチチュート　フォアー　バイオメディカル　リサーチ 腫瘍に対する遺伝子マーカー
CA2426824A1 (en) 2000-10-24 2002-07-25 The Board Of Trustees Of The Leland Stanford Junior University Direct multiplex characterization of genomic dna
US20050021240A1 (en) 2000-11-02 2005-01-27 Epigenomics Ag Systems, methods and computer program products for guiding selection of a therapeutic treatment regimen based on the methylation status of the DNA
EP2298878A3 (en) 2000-11-03 2011-11-16 Dana Farber Cancer Institute Compositions and methods for the diagnosis of cancer
EP1207209A3 (en) 2000-11-09 2002-09-11 Agilent Technologies, Inc. (a Delaware corporation) Methods using arrays for detection of single nucleotide polymorphisms
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
NZ546251A (en) 2000-12-01 2008-01-31 Response Genetics Inc Method of determining epidermal growth factor receptor gene expression in a parafin embedded tiasue sample and correlation of levels thereof with survival rates
US6958217B2 (en) 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US20020115073A1 (en) 2001-02-16 2002-08-22 Nickolas Papadopoulos Genome-based personalized medicine
DE60214604T2 (de) 2001-02-23 2006-12-28 Japan Science And Technology Agency, Kawaguchi Vorrichtung und Verfahren zum Herstellen von Mikrokapseln
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US20040110193A1 (en) 2001-07-31 2004-06-10 Gene Logic, Inc. Methods for classification of biological data
US20030165940A1 (en) 2001-12-06 2003-09-04 The Johns Hopkins University Disease detection by digital protein truncation assays
AU2002359645A1 (en) 2001-12-07 2003-07-09 University Of Massachusetts Targeted genetic risk-stratification using microarrays
US6949342B2 (en) 2001-12-21 2005-09-27 Whitehead Institute For Biomedical Research Prostate cancer diagnosis and outcome prediction by expression analysis
NZ532780A (en) 2001-12-21 2006-10-27 Wellcome Trust Isolated naturally occurring mutant human B-Raf polypeptides and nucleic acids encoding them
US7730063B2 (en) 2002-12-10 2010-06-01 Asset Trust, Inc. Personalized medicine service
ES2329565T3 (es) 2002-03-01 2009-11-27 Siemens Healthcare Diagnostics Inc. Pruebas para monitorear pacientes con cancer, basadas en los niveles de analito de dominio extracelular (ecd) del factor receptor del crecimiento epidermico (egfr),solo o en combinacion con otros analitos,en muestras de fluidos corporales.
AU2003213836A1 (en) 2002-03-11 2003-09-29 Epoch Biosciences, Inc. Negatively charged minor groove binders
US7427480B2 (en) 2002-03-26 2008-09-23 Perlegen Sciences, Inc. Life sciences business systems and methods
US7138226B2 (en) 2002-05-10 2006-11-21 The University Of Miami Preservation of RNA and morphology in cells and tissues
WO2003102216A2 (en) * 2002-05-31 2003-12-11 Diversa Corporation Multiplexed systems for nucleic acid sequencing
US9388459B2 (en) * 2002-06-17 2016-07-12 Affymetrix, Inc. Methods for genotyping
TWI266907B (en) 2002-07-24 2006-11-21 Nitto Denko Corp Polarizing device, optical thin film using the same, and image display device using the same
JP2006500034A (ja) 2002-09-19 2006-01-05 アプレラ コーポレイション 標的を検出するための方法および組成物
AU2003260946A1 (en) 2002-09-20 2004-04-08 Prokaria Ehf. Thermostable rna ligase from thermus phage
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
EP1570080A4 (en) 2002-11-15 2006-03-01 Genomic Health Inc GENE EXPRESSION PROFILING OF EGFR-POSITIVE CANCER DISEASE
US8275554B2 (en) 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
EP1587940A4 (en) 2002-12-20 2006-06-07 Caliper Life Sciences Inc SINGLE MOLECULAR AMPLIFICATION AND DNA PROOF
US20040137539A1 (en) 2003-01-10 2004-07-15 Bradford Sherry A. Cancer comprehensive method for identifying cancer protein patterns and determination of cancer treatment strategies
ATE437945T1 (de) 2003-01-29 2009-08-15 454 Corp Verfahren zur amplifikation und sequenzierung von nukleinsäuren
US20090124514A1 (en) * 2003-02-26 2009-05-14 Perlegen Sciences, Inc. Selection probe amplification
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20080044813A1 (en) 2003-03-20 2008-02-21 Kristian Jansson Amp Ligation Assay (Ala)
EP3261006A1 (en) 2003-04-09 2017-12-27 Omicia Inc. Methods of selection, reporting and analysis of genetic markers using broad based genetic profiling applications
MXPA05011404A (es) 2003-04-25 2006-05-31 Johnson & Johnson Conservacion del arn en una muestra biologica.
WO2004111603A2 (en) 2003-05-28 2004-12-23 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
RU2392324C2 (ru) * 2003-09-18 2010-06-20 Симфоген А/С Способ связывания интересующих последовательностей
CA2543786A1 (en) 2003-10-24 2005-05-06 Mmi Genomics, Inc. Methods and systems for inferring traits to manage non-beef livestock
CA2542768A1 (en) 2003-10-28 2005-05-12 Epoch Biosciences, Inc. Fluorescent probes for dna detection by hybridization with improved sensitivity and low background
US7981618B2 (en) 2003-11-05 2011-07-19 Board Of Regents Of The University Of Texas System Diagnostic and therapeutic methods and compositions involving PTEN and breast cancer
US7439341B2 (en) 2003-11-14 2008-10-21 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
US20050181377A1 (en) 2004-02-13 2005-08-18 Markovic Svetomir N. Targeted cancer therapy
US20060195266A1 (en) 2005-02-25 2006-08-31 Yeatman Timothy J Methods for predicting cancer outcome and gene signatures for use therein
US20060046258A1 (en) 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
JP2007527241A (ja) 2004-03-01 2007-09-27 ユニバーシティ オブ シカゴ 上皮細胞成長因子受容体遺伝子プロモーターにおける多型
DE602005014453D1 (de) 2004-03-24 2009-06-25 Applied Biosystems Llc Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
CN104480200B (zh) 2004-03-31 2017-12-29 综合医院公司 测定癌症对表皮生长因子受体靶向性治疗反应性的方法
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
EP2947160B1 (en) 2004-04-09 2017-07-12 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
WO2005108583A1 (en) 2004-05-06 2005-11-17 Prokaria Ehf. Thermostable polypeptide having polynucleotide kinase activity and/or phosphatase activity
US7622281B2 (en) * 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
ES2564127T5 (es) 2004-06-04 2020-03-26 Genentech Inc Mutaciones en EGFR
US7329495B2 (en) 2004-06-09 2008-02-12 Board Of Regents, The University Of Texas System Mutations in KIT confer imatinib resistance in gastrointestinal stromal tumors
AU2005267148A1 (en) 2004-07-01 2006-02-02 University Of Southern California Genetic markers for predicting disease and treatment outcome
EP1784646B1 (en) 2004-08-11 2012-06-13 Albert Einstein College Of Medicine Of Yeshiva University Method for identifying metastasis in motile cells
ATE494295T1 (de) 2004-10-18 2011-01-15 Univ Brandeis Verfahren zur nukleinsäureamplifikation
US7700283B2 (en) 2004-10-21 2010-04-20 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US20060278241A1 (en) 2004-12-14 2006-12-14 Gualberto Ruano Physiogenomic method for predicting clinical outcomes of treatments in patients
US20060184489A1 (en) 2004-12-17 2006-08-17 General Electric Company Genetic knowledgebase creation for personalized analysis of medical conditions
EP1851335B1 (en) 2005-01-21 2014-11-26 Gene Express, Inc. Compositions and methods of use of standardized mixtures
US7442507B2 (en) 2005-01-24 2008-10-28 New York University School Of Medicine Methods for detecting circulating mutant BRAF DNA
PL1859330T3 (pl) 2005-01-28 2013-01-31 Univ Duke Urządzenia i sposoby manipulacji kropelkami na płytkach obwodów drukowanych
WO2006107854A2 (en) 2005-04-01 2006-10-12 Amgen Inc. Epidermal growth factor receptor gene copy number
EP1712639B1 (en) 2005-04-06 2008-08-27 Maurice Stroun Method for the diagnosis of cancer by detecting circulating DNA and RNA
KR20080003422A (ko) 2005-04-14 2008-01-07 메르크 파텐트 게엠베하 종양 조직 내의 ｅｇｆｒ 유전자의 증가된 복제수에 기초한항-ｅｇｆｒ 항체 치료
JP4547301B2 (ja) 2005-05-13 2010-09-22 株式会社日立ハイテクノロジーズ 液体搬送デバイス及び分析システム
US20070020657A1 (en) 2005-05-20 2007-01-25 Grebe Stefan K Methods for detecting circulating tumor cells
WO2006128463A2 (en) 2005-05-31 2006-12-07 Dako Denmark A/S Compositions and methods for predicting outcome of treatment
US20090202989A1 (en) 2005-06-28 2009-08-13 Hillan Kenneth J Egfr and kras mutations
US7993821B2 (en) 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070117121A1 (en) 2005-09-16 2007-05-24 Hutchison Stephen K cDNA library preparation
CA2623445A1 (en) 2005-09-21 2007-03-29 Ccc Diagnostics, Llc Comprehensive diagnostic testing procedures for personalized anticancer chemotherapy (pac)
CA2624086A1 (en) 2005-09-28 2007-04-05 H. Lee Moffitt Cancer Center Individualized cancer treatments
PL1948816T3 (pl) 2005-10-24 2012-07-31 Univ Johns Hopkins Ulepszone sposoby beamingu
CN101365803B (zh) 2005-12-22 2013-03-20 关键基因股份有限公司 采用高通量测序技术的改进的转录谱描述策略
WO2007091228A1 (en) 2006-02-07 2007-08-16 Stokes Bio Limited A liquid bridge and system
US20100304446A1 (en) 2006-02-07 2010-12-02 Stokes Bio Limited Devices, systems, and methods for amplifying nucleic acids
EP1991357B1 (en) 2006-02-07 2016-09-14 Stokes Bio Limited A microfluidic analysis system
WO2007095644A2 (en) 2006-02-16 2007-08-23 Ventana Medical Systems, Inc. Reagents and methods for cancer prognosis and pathological staging
EP1991698B1 (en) 2006-03-01 2013-12-18 Keygene N.V. High throughput sequence-based detection of snps using ligation assays
US8165367B2 (en) 2006-03-08 2012-04-24 Olympus Medical Systems Corp. Medical image processing apparatus and medical image processing method having three-dimensional model estimating
WO2007106432A2 (en) 2006-03-10 2007-09-20 George Mason Intellectual Properties, Inc. Egf receptor phosphorylation status for disease treatment
US7908091B2 (en) 2006-03-17 2011-03-15 Prometheus Laboratories Inc. Methods of predicting and monitoring tyrosine kinase inhibitor therapy
WO2007111937A1 (en) 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
CA2647102A1 (en) 2006-03-27 2007-11-22 Globeimmune, Inc. Ras mutation and compositions and methods related thereto
TWI395754B (zh) 2006-04-24 2013-05-11 Amgen Inc 人類化之ｃ-ｋｉｔ抗體
WO2007133506A2 (en) 2006-05-09 2007-11-22 University Of Louisville Research Foundation , Inc. Personalized medicine management software
EP2481815B1 (en) 2006-05-11 2016-01-27 Raindance Technologies, Inc. Microfluidic devices
US8768629B2 (en) 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
CN103424541B (zh) 2006-05-18 2018-01-30 分子压型学会股份有限公司 确定针对病状的个性化医疗介入的系统和方法
WO2008111990A1 (en) 2006-06-14 2008-09-18 Cellpoint Diagnostics, Inc. Rare cell analysis using sample splitting and dna tags
EP2049682A2 (en) 2006-07-31 2009-04-22 Illumina Cambridge Limited Method of library preparation avoiding the formation of adaptor dimers
US20080065411A1 (en) 2006-09-08 2008-03-13 Diaceutics Method and system for developing a personalized medicine business plan
SG174826A1 (en) 2006-09-19 2011-10-28 Novartis Ag Biomarkers of target modulation, efficacy, diagnosis and/or prognosis for raf inhibitors
US20100075312A1 (en) 2006-09-28 2010-03-25 Stokes Bio Limited Qpcr analysis apparatus
US8568979B2 (en) 2006-10-10 2013-10-29 Illumina, Inc. Compositions and methods for representational selection of nucleic acids from complex mixtures using hybridization
NZ576591A (en) 2006-10-27 2012-04-27 Decode Genetics Efh Cancer susceptibility variants on chr8q24.21
KR20090095571A (ko) 2006-11-01 2009-09-09 조지 매이슨 인털렉춸 프로퍼티즈, 인코퍼레이티드 암을 검출하고 조절하기 위한 방법
US20100196889A1 (en) 2006-11-13 2010-08-05 Bankaitis-Davis Danute M Gene Expression Profiling for Identification, Monitoring and Treatment of Colorectal Cancer
US20080131887A1 (en) 2006-11-30 2008-06-05 Stephan Dietrich A Genetic Analysis Systems and Methods
WO2008115300A1 (en) 2006-12-01 2008-09-25 Apocell, Inc. C-kit phosphorylation in cancer
CA2672315A1 (en) 2006-12-14 2008-06-26 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20080177608A1 (en) 2007-01-19 2008-07-24 Diaceutics Business method for enabling personalized medicine
SG10201506658TA (en) 2007-02-26 2015-09-29 Wayne John Cancer Inst Utility of b-raf dna mutation in diagnosis and treatment of cancer
US20100166747A1 (en) 2007-03-02 2010-07-01 Beltran Pedro J Methods and compositions for treating tumor diseases
RS52932B (sr) 2007-03-13 2014-02-28 Amgen Inc. Mutacije k-ras i b-raf i lečenje anti-egfr antitelima
HRP20140360T4 (hr) 2007-03-13 2022-06-10 Amgen, Inc. K-ras mutacije i terapija anti-egfr antitijelom
WO2008115419A2 (en) 2007-03-15 2008-09-25 Genomic Health, Inc. Gene expression markers for prediction of patient response to chemotherapy
WO2008115556A2 (en) 2007-03-19 2008-09-25 Cold Spring Harbor Laboratory Identification of genetic alterations that modulate drug sensitivity in cancer treatments
CA2683804A1 (en) 2007-04-13 2008-10-23 Dana Farber Cancer Institute, Inc. Receptor tyrosine kinase profiling
CA2683625A1 (en) 2007-04-13 2008-10-23 George Mason Intellectual Properties, Inc. Stat3 as a theranostic indicator
WO2008147879A1 (en) 2007-05-22 2008-12-04 Ryan Golhar Automated method and device for dna isolation, sequence determination, and identification
WO2008148072A2 (en) 2007-05-24 2008-12-04 The Brigham And Women's Hospital, Inc. Disease-associated genetic variations and methods for obtaining and using same
AU2008265691B2 (en) 2007-06-19 2014-04-24 F. Hoffmann-La Roche Ag High throughput nucleic acid sequencing by expansion
GB0712882D0 (en) * 2007-07-03 2007-08-15 Leicester University Of Nucleic acid amplification
MX2010000405A (es) 2007-07-13 2010-03-04 Prometheus Lab Inc Seleccion de farmacos para terapia de cancer de pulmon usando arreglos a base de anticuerpos.
KR20220146689A (ko) 2007-07-23 2022-11-01 더 차이니즈 유니버시티 오브 홍콩 핵산 서열 불균형의 결정
US8043815B2 (en) 2007-08-06 2011-10-25 Health Research, Inc. Methods for analysis of PDEF and survivin as interconnected cancer biomarkers and targets for personalized medicine
US20090062138A1 (en) 2007-08-31 2009-03-05 Curry Bo U Array-based method for performing SNP analysis
US20110212991A1 (en) 2007-09-11 2011-09-01 Roche Molecular Systems, Inc. Diagnostic Test for Susceptibility to B-RAF Kinase Inhibitors
US20100173294A1 (en) 2007-09-11 2010-07-08 Roche Molecular Systems, Inc. Diagnostic test for susceptibility to b-raf kinase inhibitors
US20090209436A1 (en) * 2007-09-17 2009-08-20 Twof, Inc. Hydrogel labeled primer extension method for microarrays
CA2701726A1 (en) 2007-10-04 2009-04-09 Halcyon Molecular Sequencing nucleic acid polymers with electron microscopy
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
US20090264298A1 (en) 2007-11-06 2009-10-22 Ambergen, Inc. Methods for enriching subpopulations
EP2063132A1 (de) * 2007-11-24 2009-05-27 Festo AG & Co. KG Linearantriebsvorrichtung
US8530197B2 (en) 2008-01-09 2013-09-10 Applied Biosystems, Llc Method of making a paired tag library for nucleic acid sequencing
EP2245191A1 (en) 2008-01-17 2010-11-03 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
US11051733B2 (en) 2008-01-18 2021-07-06 Wake Forest University Health Sciences Isolating and purifying cells for therapy
US20110053157A1 (en) 2008-02-01 2011-03-03 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
WO2009099602A1 (en) 2008-02-04 2009-08-13 Massachusetts Institute Of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
EP2247755B1 (en) 2008-02-14 2015-01-28 Decode Genetics EHF Susceptibility variants for lung cancer
WO2009102957A2 (en) 2008-02-14 2009-08-20 The Johns Hopkins University Methods to connect gene set expression profiles to drug sensitivity
AU2009219437B2 (en) 2008-02-25 2014-10-16 Nestec S.A. Drug selection for breast cancer therapy using antibody-based arrays
US20090226975A1 (en) * 2008-03-10 2009-09-10 Illumina, Inc. Constant cluster seeding
WO2009114836A1 (en) 2008-03-14 2009-09-17 Genomic Health, Inc. Gene expression markers for prediction of patient response to chemotherapy
CA2718887A1 (en) 2008-03-19 2009-09-24 Existence Genetics Llc Genetic analysis
US7842248B2 (en) 2008-06-20 2010-11-30 Silverbrook Research Pty Ltd Microfluidic system comprising microfluidic pump, mixer or valve
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
US20100041048A1 (en) 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
JP2010041985A (ja) * 2008-08-18 2010-02-25 Hitachi Plant Technologies Ltd 核酸配列増幅方法、核酸配列の検出方法及び核酸増幅・検出用基板
EP3216874A1 (en) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Methods for stratifying and annotating cancer drug treatment options
AU2009297108B2 (en) 2008-09-23 2015-02-12 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20110159499A1 (en) 2009-11-25 2011-06-30 Quantalife, Inc. Methods and compositions for detecting genetic material
CA2739675C (en) 2008-10-14 2020-12-01 Caris Mpi, Inc. Gene and gene expressed protein targets depicting biomarker patterns and signature sets by tumor type
US10262103B2 (en) 2008-11-18 2019-04-16 Raphael LEHRER Individualized cancer treatment
US8349563B2 (en) 2008-11-18 2013-01-08 Life Technologies Corporation Sequence amplification with target primers
CA2747026A1 (en) 2008-12-17 2010-07-08 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
US8790873B2 (en) 2009-01-16 2014-07-29 Affymetrix, Inc. DNA ligation on RNA template
JP2012517813A (ja) 2009-02-16 2012-08-09 エピセンター テクノロジーズ コーポレイション １本鎖ｄｎａの鋳型非依存性ライゲーション
US20100286143A1 (en) 2009-04-24 2010-11-11 Dora Dias-Santagata Methods and materials for genetic analysis of tumors
EP2430441B1 (en) 2009-04-29 2018-06-13 Complete Genomics, Inc. Method and system for calling variations in a sample polynucleotide sequence with respect to a reference polynucleotide sequence
DK2669387T3 (en) * 2009-08-25 2016-09-19 Illumina Inc Methods of selection and amplification of polynucleotides
WO2011032040A1 (en) 2009-09-10 2011-03-17 Centrillion Technology Holding Corporation Methods of targeted sequencing
WO2011050981A2 (en) * 2009-10-30 2011-05-05 Roche Diagnostics Gmbh Method for detecting balanced chromosomal aberrations in a genome
US20110117559A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US8964013B2 (en) 2009-12-31 2015-02-24 Broadcom Corporation Display with elastic light manipulator
EP4435111A1 (en) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digital analyte analysis
DK2539450T3 (da) 2010-02-25 2016-05-30 Advanced Liquid Logic Inc Fremgangsmåde til fremstilling af nukleinsyrebiblioteker
WO2011123749A1 (en) 2010-04-01 2011-10-06 New England Biolabs, Inc. Compositions and methods for adenylating oligonucleotides
WO2011140510A2 (en) 2010-05-06 2011-11-10 Bioo Scientific Corporation Oligonucleotide ligation, barcoding and methods and compositions for improving data quality and throughput using massively parallel sequencing
US20110299645A1 (en) 2010-06-07 2011-12-08 Korea Hydro & Nuclear Power Co., Ltd. Breeding Nuclear Fuel Mixture Using Metallic Thorium
US20120003657A1 (en) 2010-07-02 2012-01-05 Samuel Myllykangas Targeted sequencing library preparation by genomic dna circularization
EP2598660B1 (en) 2010-07-26 2017-03-15 Biomatrica, INC. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
IN2013MN00522A (enExample) 2010-09-24 2015-05-29 Univ Leland Stanford Junior
WO2012054933A2 (en) 2010-10-22 2012-04-26 Fluidigm Corporation Universal probe assay methods
EP3147374B1 (en) 2010-12-17 2019-01-30 Life Technologies Corporation Methods for nucleic acid amplification
WO2012092426A1 (en) 2010-12-30 2012-07-05 Foundation Medicine, Inc. Optimization of multigene analysis of tumor samples
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
CN110016499B (zh) 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序系统
WO2013036810A1 (en) 2011-09-07 2013-03-14 X-Chem, Inc. Methods for tagging dna-encoded libraries
US20140303008A1 (en) 2011-10-21 2014-10-09 Chronix Biomedical Colorectal cancer associated circulating nucleic acid biomarkers
CA2862552A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP3584327A1 (en) 2012-01-27 2019-12-25 The Board of Trustees of the Leland Stanford Junior University Methods for profiling and quantitating cell-free rna
US20130203635A1 (en) 2012-02-06 2013-08-08 Ronald T. Raines Nucleic acid ligation method
WO2013173472A1 (en) 2012-05-15 2013-11-21 Predictive Biosciences, Inc. Methods of assessing chromosomal instabilities
US11261494B2 (en) 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
US9816120B2 (en) 2013-01-09 2017-11-14 The Penn State Research Foundation Low sequence bias single-stranded DNA ligation
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
HK1217971A1 (zh) 2013-02-21 2017-01-27 Toma Biosciences Inc 用於核酸分析的方法、組合物和試劑盒
US20140287937A1 (en) 2013-02-21 2014-09-25 Toma Biosciences, Inc. Methods for assessing cancer
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
MX376900B (es) 2013-12-11 2025-03-04 Accuragen Holdings Ltd Composiciones y metodos para detectar variantes poco frecuentes de una secuencia.
US20190050530A1 (en) 2016-02-09 2019-02-14 Toma Biosciences, Inc. Systems and Methods for Analyzing Nucleic Acids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080286795A1 (en) * 1997-04-01 2008-11-20 Solexa Limited Method of nucleic acid amplification
US20090117621A1 (en) * 2005-07-20 2009-05-07 Jonathan Mark Boutell Methods of nucleic acid amplification and sequencing
US20090117573A1 (en) * 2007-09-14 2009-05-07 Affymetrix, Inc. Locus specific amplification using array probes
US20100137143A1 (en) * 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes

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