SI1687609T1 - Fluorescenäśne sonde za detekcijo dna s hibridizacijo z izboljĺ ano obäśutljivostjo in nizkim ozadjem - Google Patents

Fluorescenäśne sonde za detekcijo dna s hibridizacijo z izboljĺ ano obäśutljivostjo in nizkim ozadjem

Info

Publication number
SI1687609T1
SI1687609T1 SI200432218T SI200432218T SI1687609T1 SI 1687609 T1 SI1687609 T1 SI 1687609T1 SI 200432218 T SI200432218 T SI 200432218T SI 200432218 T SI200432218 T SI 200432218T SI 1687609 T1 SI1687609 T1 SI 1687609T1
Authority
SI
Slovenia
Prior art keywords
hybridization
fluorescent probes
improved sensitivity
low background
dna detection
Prior art date
Application number
SI200432218T
Other languages
English (en)
Inventor
Sergey Lokhov
Eugene Lukhtanov
Original Assignee
Epoch Biosciences, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epoch Biosciences, Inc. filed Critical Epoch Biosciences, Inc.
Publication of SI1687609T1 publication Critical patent/SI1687609T1/sl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SI200432218T 2003-10-28 2004-10-27 Fluorescenäśne sonde za detekcijo dna s hibridizacijo z izboljĺ ano obäśutljivostjo in nizkim ozadjem SI1687609T1 (sl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51552303P 2003-10-28 2003-10-28
EP04817540.0A EP1687609B1 (en) 2003-10-28 2004-10-27 Fluorescent probes for dna detection by hybridization with improved sensitivity and low background
PCT/US2004/037981 WO2005043127A2 (en) 2003-10-28 2004-10-27 Fluorescent probes for dna detection by hybridization with improved sensitivity and low background

Publications (1)

Publication Number Publication Date
SI1687609T1 true SI1687609T1 (sl) 2015-03-31

Family

ID=34549422

Family Applications (1)

Application Number Title Priority Date Filing Date
SI200432218T SI1687609T1 (sl) 2003-10-28 2004-10-27 Fluorescenäśne sonde za detekcijo dna s hibridizacijo z izboljĺ ano obäśutljivostjo in nizkim ozadjem

Country Status (11)

Country Link
US (2) US7381818B2 (sl)
EP (1) EP1687609B1 (sl)
JP (1) JP4942484B2 (sl)
AU (1) AU2004286352A1 (sl)
CA (1) CA2542768A1 (sl)
DK (1) DK1687609T3 (sl)
ES (1) ES2529256T3 (sl)
PL (1) PL1687609T3 (sl)
PT (1) PT1687609E (sl)
SI (1) SI1687609T1 (sl)
WO (1) WO2005043127A2 (sl)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1894423A (zh) 2003-05-20 2007-01-10 因维斯蒂恩有限公司 检测多核苷酸的系统
CA2542768A1 (en) * 2003-10-28 2005-05-12 Epoch Biosciences, Inc. Fluorescent probes for dna detection by hybridization with improved sensitivity and low background
WO2007100711A2 (en) * 2006-02-24 2007-09-07 Investigen, Inc. Methods and compositions for detecting polynucleotides
EP2004861A2 (en) * 2006-04-04 2008-12-24 Arcxis Biotechnologies, Inc. Cooperative probes and methods of using them
US8541555B2 (en) * 2006-04-18 2013-09-24 Solulink Biosciences, Inc. Hydrazone-based and oxime-based fluorescent and chromophoric/pro-fluorescent and pro-chromophoric reagents and linkers
US20090092967A1 (en) * 2006-06-26 2009-04-09 Epoch Biosciences, Inc. Method for generating target nucleic acid sequences
US7771947B2 (en) 2007-02-23 2010-08-10 Investigen, Inc. Methods and compositions for rapid light-activated isolation and detection of analytes
US20090111100A1 (en) * 2007-10-30 2009-04-30 Eugene Lukhtanov Minor groove binder - energy transfer oligonucleotides and methods for their use
WO2010011884A2 (en) * 2008-07-25 2010-01-28 University Of Florida Research Foundation, Inc. Novel nucleic acid-based molecular probes
WO2010028288A2 (en) 2008-09-05 2010-03-11 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
CA2748174A1 (en) 2008-12-30 2010-07-08 Arqule, Inc. Substituted 1h-pyrazolo[3,4-d]pyrimidine-6-amine compounds
AU2010232727A1 (en) 2009-03-31 2011-10-20 Arqule, Inc. Substituted heterocyclic compounds
CN101659952B (zh) * 2009-07-14 2012-07-18 上海之江生物科技有限公司 锁核酸和小沟结合物共修饰核酸片段
JP2013511984A (ja) 2009-11-25 2013-04-11 エリテック・ホールディング・ベスローテン・フェンノートシャップ 副溝結合剤(MGB)−オリゴヌクレオチドmiRNAアンタゴニスト
KR101569476B1 (ko) * 2009-12-21 2015-11-16 주식회사 씨젠 Tsg프라이머 타겟 검출
WO2011087707A1 (en) 2009-12-22 2011-07-21 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
MX346956B (es) 2010-09-24 2017-04-06 Univ Leland Stanford Junior Captura directa, amplificación y secuenciación de objetivo adn usando cebadores inmovilizados.
AU2012223237A1 (en) * 2011-03-02 2013-05-02 Groove Biopharma Corporation Enhanced biodistribution of oligomers
US8969003B2 (en) 2011-03-23 2015-03-03 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
WO2012129547A1 (en) * 2011-03-23 2012-09-27 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
DK2801626T3 (en) 2011-05-24 2016-06-20 Elitechgroup B V Detection of methicillin-resistant Staphylococcus aureus.
WO2013016321A1 (en) 2011-07-26 2013-01-31 Elitech Holding B.V. Minor groove binder phosphoramidites and methods of use
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
ES2654631T3 (es) 2013-05-13 2018-02-14 Elitechgroup B.V. PCR digital en gotas con sondas cortas de ranura menor
JP5928906B2 (ja) * 2013-08-27 2016-06-01 横河電機株式会社 核酸配列計測方法、核酸配列計測用デバイス、核酸配列計測用デバイスの製造方法および核酸配列計測装置
WO2016094607A2 (en) 2014-12-12 2016-06-16 Elitechgroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
US10266903B2 (en) 2014-12-12 2019-04-23 Elitechgroup, Inc. Methods and compositions for detecting antibiotic resistant bacteria
EP3208260A1 (en) * 2016-02-17 2017-08-23 Clariant International Ltd Alkoxylated hydroxybenzoic acid esters or amides
US10718017B2 (en) 2016-03-31 2020-07-21 Arizona Board Of Regents On Behalf Of Arizona State University Probes and methods for measuring tandem repeats
IL291323B2 (en) 2016-07-15 2023-09-01 Am Chemicals Llc Solid non-nucleoside supports and phosphoramidite building blocks for oligonucleotide synthesis
US10738346B2 (en) 2017-03-09 2020-08-11 Elitechgroup, Inc. Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes
ES2909351T3 (es) 2017-08-17 2022-05-06 Elitechgroup Inc Quencheres estabilizadores de fluorescencia dúplex para sondas de ácidos nucleicos
WO2019178337A1 (en) 2018-03-14 2019-09-19 Luminex Corporation Multiplex amplification of nucleic acids employing lowering of denaturation temperature and increasing the annealing temperature
EP3802526A1 (en) 2018-05-29 2021-04-14 ELITechGroup, Inc. Carborhodamine compounds and methods of preparation thereof
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2023248185A1 (en) 2022-06-24 2023-12-28 Mobidiag Oy Compact detection system

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
FR2540122B1 (fr) * 1983-01-27 1985-11-29 Centre Nat Rech Scient Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の5−及び6−スクシニミジルカルボキシレ―ト異性体
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5525464A (en) * 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5202231A (en) * 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1990003370A1 (en) 1988-09-28 1990-04-05 Microprobe Corporation DERIVATIVES OF PYRAZOLO[3,4-d]PYRIMIDINE
US5849482A (en) 1988-09-28 1998-12-15 Epoch Pharmaceuticals, Inc. Crosslinking oligonucleotides
USRE38416E1 (en) 1988-09-28 2004-02-03 Epoch Biosciences, Inc. Cross-linking oligonucleotides
EP0472648A4 (en) 1989-05-18 1992-09-16 Microprobe Corporation Crosslinking oligonucleotides
US5451463A (en) 1989-08-28 1995-09-19 Clontech Laboratories, Inc. Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5237101A (en) * 1990-01-26 1993-08-17 The Trustees Of The Univ. Of Penna. Propargylic and allenic sulfones
WO1992000588A1 (en) 1990-07-02 1992-01-09 Teijin Limited Optical disk
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
AU2028792A (en) 1991-05-17 1992-12-30 Uab Research Foundation Sequence specific dna binding drugs
US5419966A (en) 1991-06-10 1995-05-30 Microprobe Corporation Solid support for synthesis of 3'-tailed oligonucleotides
US5512677A (en) 1991-08-13 1996-04-30 National Science Council 3-substituted methyl-2,3-dihydroimidazo 1,2-c!quinazoline derivatives, the preparation and use thereof
EP0661979A4 (en) 1991-08-21 1995-09-13 Microprobe Corp CROSSLINKED OLIGONUCLEOTIDES FOR ENZYMATIC MEDIATED TRIPLE STRAND FORMATION.
JP2775552B2 (ja) 1991-12-26 1998-07-16 三菱電機株式会社 半導体記憶装置
US5574142A (en) * 1992-12-15 1996-11-12 Microprobe Corporation Peptide linkers for improved oligonucleotide delivery
WO1994017092A1 (en) 1993-01-26 1994-08-04 Microprobe Corporation Bifunctional crosslinking oligonucleotides adapted for linking to a desired gene sequence of invading organism or cell
US5786138A (en) * 1993-01-29 1998-07-28 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Hyperstabilizing antisense nucleic acid binding agents
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5446137B1 (en) * 1993-12-09 1998-10-06 Behringwerke Ag Oligonucleotides containing 4'-substituted nucleotides
US5646126A (en) * 1994-02-28 1997-07-08 Epoch Pharmaceuticals Sterol modified oligonucleotide duplexes having anticancer activity
DE4408528A1 (de) 1994-03-14 1995-09-28 Hoechst Ag Peptid-Oligonucleotid-Derivate, deren Herstellung und Verwendung
FR2719048B1 (fr) 1994-04-25 1996-07-19 Pasteur Strasbourg I Universit Bases nucléiques polycationiques et oligonucléotides les contenant.
US5556752A (en) * 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5912340A (en) 1995-10-04 1999-06-15 Epoch Pharmaceuticals, Inc. Selective binding complementary oligonucleotides
US5659022A (en) * 1996-01-05 1997-08-19 Epoch Pharmaceuticals, Inc. Oligonucleotide-cyclopropapyrroloindole conjugates as sequence specific hybridization and crosslinking agents for nucleic acids
US5736626A (en) 1996-01-29 1998-04-07 The Perkin-Elmer Corporation Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
US5776907A (en) * 1996-05-20 1998-07-07 Texas Biotechnology Corporation Mitomycin oligonucleotide conjugates
US5955590A (en) 1996-07-15 1999-09-21 Worcester Foundation For Biomedical Research Conjugates of minor groove DNA binders with antisense oligonucleotides
AU5152798A (en) * 1996-10-29 1998-05-22 University Of Nebraska-Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
US7045610B2 (en) 1998-04-03 2006-05-16 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
US6127121A (en) 1998-04-03 2000-10-03 Epoch Pharmaceuticals, Inc. Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination
US6683173B2 (en) 1998-04-03 2004-01-27 Epoch Biosciences, Inc. Tm leveling methods
US6486303B1 (en) 1998-04-14 2002-11-26 University Of Medicine & Dentistry Of New Jersey Method for making hormone heterodimers
US6339147B1 (en) 1999-07-29 2002-01-15 Epoch Biosciences, Inc. Attachment of oligonucleotides to solid supports through Schiff base type linkages for capture and detection of nucleic acids
US6472153B1 (en) * 1999-10-26 2002-10-29 Epoch Biosciences, Inc. Hybridization-triggered fluorescent detection of nucleic acids
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US7205105B2 (en) 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
AU2001234883A1 (en) * 2000-02-10 2001-08-20 The Penn State Research Foundation Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion
ES2391213T3 (es) * 2000-03-01 2012-11-22 Epoch Biosciences, Inc. Oligonucle�tidos modificados para discriminaci�n de desapareamiento
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US6972339B2 (en) 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US6962991B2 (en) 2001-09-12 2005-11-08 Epoch Biosciences, Inc. Process for the synthesis of pyrazolopyrimidines
WO2003026657A1 (en) 2001-09-24 2003-04-03 Synaptic Pharmaceutical Corporation Compounds for the treatment of pain
US7582739B2 (en) 2002-03-11 2009-09-01 Epoch Biosciences, Inc. Negatively charged minor groove binders
US7348146B2 (en) 2003-10-02 2008-03-25 Epoch Biosciences, Inc. Single nucleotide polymorphism analysis of highly polymorphic target sequences
CA2542768A1 (en) * 2003-10-28 2005-05-12 Epoch Biosciences, Inc. Fluorescent probes for dna detection by hybridization with improved sensitivity and low background

Also Published As

Publication number Publication date
ES2529256T3 (es) 2015-02-18
PT1687609E (pt) 2015-03-02
US20050214797A1 (en) 2005-09-29
EP1687609B1 (en) 2014-12-10
JP2007509634A (ja) 2007-04-19
CA2542768A1 (en) 2005-05-12
WO2005043127A3 (en) 2006-11-09
US7381818B2 (en) 2008-06-03
EP1687609A2 (en) 2006-08-09
US20080293586A1 (en) 2008-11-27
WO2005043127A2 (en) 2005-05-12
PL1687609T3 (pl) 2015-05-29
US7759126B2 (en) 2010-07-20
DK1687609T3 (en) 2015-01-12
EP1687609A4 (en) 2012-06-20
JP4942484B2 (ja) 2012-05-30
AU2004286352A1 (en) 2005-05-12

Similar Documents

Publication Publication Date Title
SI1687609T1 (sl) Fluorescenäśne sonde za detekcijo dna s hibridizacijo z izboljĺ ano obäśutljivostjo in nizkim ozadjem
HK1120568A1 (en) Biosensors for detecting macromolecules and other analytes
EP2266996A3 (en) New labelling strategies for the sensitive detection of analytes
IL187130A0 (en) Turnover probes and use thereof for nucleic acid detection
HK1162618A1 (zh) 用於檢測甲基化 的試劑盒和方法
EP1985715A3 (en) ESR1 and cervical cancer
HK1098177A1 (en) Apparatus and methods for detecting nucleic acid in biological samples
IL174599A0 (en) Systems and methods for detecting biological features
EP1855102A4 (en) FLUORESCENCE DETECTION DEVICE AND FLUORESCENCE DETECTION METHOD
HK1095381A1 (en) Sample collection cup with integrated sample analysis system
EP2484781A3 (en) Detection of analytes and nucleic acids
EP1737985A4 (en) NUCLEIC ACID DETECTION SYSTEM
EP1817573A4 (en) FLUORESCENCE DETECTION
EP1395806A4 (en) SAMPLE LEVEL DETECTION SYSTEM
EP1613959A4 (en) PROCESS FOR DETECTING CANCER CELLS IN BIOLOGICAL SAMPLES
HK1147525A1 (en) Reagents and methods for detecting analytes
EP1692266A4 (en) IMPROVED METHODS FOR DETECTING AND MEASURING SPECIFIC NUCLEIC ACID SEQUENCES
EP1620556A4 (en) NUCLEIC PROOF
HK1113405A1 (en) Multifunctional reference system for use in analyte detection by fluorescence
AU2003254298A8 (en) Species specific dna detection
ZA200601731B (en) Methods for detecting and quantifying specific microorganisms
AU2003290433A8 (en) Nucleic acid methylation detection process using an internal reference sample
GB0324650D0 (en) Using nucleic acids for clinical microbiology testing
ZA200903128B (en) A high sensitivity assay for molecular typing of biological sample, probes and a kit thereof
AU2003226642A1 (en) Stn gene oligonucleotide primers for detecting salmonella species and detection prosses using the same