AU2003289338A1 - Novel nitrile hydratase - Google Patents

Novel nitrile hydratase Download PDF

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Publication number
AU2003289338A1
AU2003289338A1 AU2003289338A AU2003289338A AU2003289338A1 AU 2003289338 A1 AU2003289338 A1 AU 2003289338A1 AU 2003289338 A AU2003289338 A AU 2003289338A AU 2003289338 A AU2003289338 A AU 2003289338A AU 2003289338 A1 AU2003289338 A1 AU 2003289338A1
Authority
AU
Australia
Prior art keywords
amino acid
sequence
subunit
nitrile hydratase
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003289338A
Other languages
English (en)
Inventor
Shinichi Banba
Kiyoshi Ito
Hideki Kobayashi
Kaori Matoishi
Toshihiro Oikawa
Eishi Tanaka
Toshifumi Yamaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsui Chemicals Inc
Original Assignee
Mitsui Chemicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsui Chemicals Inc filed Critical Mitsui Chemicals Inc
Publication of AU2003289338A1 publication Critical patent/AU2003289338A1/en
Priority to AU2010202683A priority Critical patent/AU2010202683B2/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01084—Nitrile hydratase (4.2.1.84)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2003289338A 2002-12-19 2003-12-15 Novel nitrile hydratase Abandoned AU2003289338A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2010202683A AU2010202683B2 (en) 2002-12-19 2010-06-28 Novel nitrile hydratase

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2002-368360 2002-12-19
JP2002368360A JP2004194588A (ja) 2002-12-19 2002-12-19 新規なニトリルヒドラターゼ
JP2003379280 2003-11-10
JP2003-379280 2003-11-10
PCT/JP2003/016014 WO2004056990A1 (ja) 2002-12-19 2003-12-15 新規なニトリルヒドラターゼ

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2010202683A Division AU2010202683B2 (en) 2002-12-19 2010-06-28 Novel nitrile hydratase

Publications (1)

Publication Number Publication Date
AU2003289338A1 true AU2003289338A1 (en) 2004-07-14

Family

ID=32684188

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2003289338A Abandoned AU2003289338A1 (en) 2002-12-19 2003-12-15 Novel nitrile hydratase
AU2010202683A Expired AU2010202683B2 (en) 2002-12-19 2010-06-28 Novel nitrile hydratase

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2010202683A Expired AU2010202683B2 (en) 2002-12-19 2010-06-28 Novel nitrile hydratase

Country Status (7)

Country Link
US (1) US7595184B2 (cg-RX-API-DMAC10.html)
EP (1) EP1586637B1 (cg-RX-API-DMAC10.html)
JP (1) JP2004194588A (cg-RX-API-DMAC10.html)
KR (5) KR100819228B1 (cg-RX-API-DMAC10.html)
CN (4) CN101892211A (cg-RX-API-DMAC10.html)
AU (2) AU2003289338A1 (cg-RX-API-DMAC10.html)
WO (1) WO2004056990A1 (cg-RX-API-DMAC10.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005248259B2 (en) * 2004-05-26 2010-07-08 Mitsubishi Chemical Corporation Improved nitrile hydratase

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1842907A1 (en) 2006-04-07 2007-10-10 B.R.A.I.N. Ag A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity
JP5069032B2 (ja) * 2007-04-04 2012-11-07 ダイヤニトリックス株式会社 改良型ニトリルヒドラターゼ
CN102216455B (zh) * 2008-11-14 2016-05-04 三井化学株式会社 腈水合酶突变体
JP5532618B2 (ja) * 2009-01-30 2014-06-25 三菱レイヨン株式会社 改良型ニトリルヒドラターゼ及びその製造方法
US8637277B2 (en) 2009-04-30 2014-01-28 Mitsui Chemicals, Inc. Method for producing 3-mercaptopropionic acid or salt thereof
US9388403B2 (en) 2011-05-31 2016-07-12 Mitsubishi Rayon Co., Ltd. Nitrile hydratase
AU2012265680B2 (en) 2011-06-07 2015-05-28 Mitsubishi Chemical Corporation Improved nitrile hydratase
CN102517271B (zh) * 2011-12-13 2013-04-03 清华大学 一种突变腈水合酶
EP3153582B1 (en) 2014-06-06 2020-06-17 Mitsubishi Chemical Corporation Improved nitrile hydratase
CN104725476B (zh) * 2015-03-31 2018-10-16 清华大学 一种钩状结构寡肽及其应用
EP3564368B1 (en) * 2016-12-28 2025-03-26 Mitsui Chemicals, Inc. Mutant nitrile hydratase, nucleic acid coding said mutant nitrile hydratase, expression vector and transformant including said nucleic acid, production method for said mutant nitrile hydratase, and production method for amide compound
KR20190049109A (ko) 2017-11-01 2019-05-09 (주)대화정공 풀리 제조방법
KR102068615B1 (ko) 2019-04-08 2020-01-21 (주)대화정공 자동차 댐퍼풀리 제조방법
KR102146408B1 (ko) 2019-04-08 2020-08-21 (주)대화정공 단류선 개선을 위해 압연롤러를 이용한 자동차 댐퍼풀리 제조방법
CN111690675B (zh) * 2019-08-01 2022-07-19 安徽瑞邦生物科技有限公司 一种表达腈水合酶突变体的重组菌及其制备方法和应用
CN114790452B (zh) * 2020-11-20 2024-03-26 江南大学 一种对腈类化合物具有高稳定性的腈水合酶突变体
CN112522245A (zh) * 2020-12-09 2021-03-19 江南大学 一种腈水合酶氨基酸基序的改造及其应用
CN113151234B (zh) * 2021-04-13 2022-08-12 浙江工业大学 腈水合酶赖氨酸突变体hba-k2h2r、编码基因及应用
CN113444714B (zh) * 2021-07-29 2022-06-21 浙江大学杭州国际科创中心 一种β亚基突变的腈水合酶突变体及其应用
CN114317507B (zh) * 2021-11-30 2024-07-02 清华大学 腈水合酶突变体及其应用
CN119343452A (zh) * 2022-06-30 2025-01-21 三井化学株式会社 突变型腈水合酶、编码该突变型腈水合酶的核酸、含有该核酸的载体及转化体、该突变型腈水合酶的制造方法、及酰胺化合物的制造方法
CN115772514B (zh) * 2022-11-03 2025-03-04 无锡新晨宇生物工程有限公司 一种腈水合酶底物通道氨基酸基序的改造用于肉桂酰胺的制备
EP4600368A1 (en) * 2024-02-09 2025-08-13 Adisseo France S.A.S. A method for enzymatic production of an amide compound

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX169933B (es) 1987-09-18 1993-08-02 Hideaki Yamada Procedimiento para la produccion biologica de amidas
AU627648B2 (en) 1990-02-28 1992-08-27 Teruhiko Beppu Dna fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
JPH0856684A (ja) 1994-08-16 1996-03-05 Mitsui Toatsu Chem Inc 微生物によるアミド化合物の製造法
EP0790310B1 (en) * 1996-02-14 2008-10-08 Mitsui Chemicals, Inc. Nitrile hydratase, derivatives thereof and methods of producing compounds
JP3380133B2 (ja) 1996-02-14 2003-02-24 三井化学株式会社 新規なニトリルヒドラターゼ
JP3408737B2 (ja) 1998-03-16 2003-05-19 三井化学株式会社 ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005248259B2 (en) * 2004-05-26 2010-07-08 Mitsubishi Chemical Corporation Improved nitrile hydratase

Also Published As

Publication number Publication date
EP1586637A4 (en) 2008-03-19
CN1729288A (zh) 2006-02-01
AU2010202683A1 (en) 2010-07-15
KR20070007393A (ko) 2007-01-15
WO2004056990A1 (ja) 2004-07-08
KR100819228B1 (ko) 2008-04-03
CN101892211A (zh) 2010-11-24
KR100794001B1 (ko) 2008-01-10
KR100825147B1 (ko) 2008-04-24
US20070009985A1 (en) 2007-01-11
US7595184B2 (en) 2009-09-29
KR100785548B1 (ko) 2007-12-12
EP1586637B1 (en) 2014-02-26
KR20050089972A (ko) 2005-09-09
JP2004194588A (ja) 2004-07-15
AU2010202683B2 (en) 2012-01-19
KR20070087054A (ko) 2007-08-27
KR20070011620A (ko) 2007-01-24
CN103103175A (zh) 2013-05-15
CN103215242A (zh) 2013-07-24
KR20070110146A (ko) 2007-11-15
KR100785549B1 (ko) 2007-12-12
EP1586637A1 (en) 2005-10-19

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