ATE554172T1 - Proteinfragmente die polyepitope der proteine e6 und e7 von hpv umfassen, deren herstellung und verwendung insbesondere zur impfung - Google Patents

Proteinfragmente die polyepitope der proteine e6 und e7 von hpv umfassen, deren herstellung und verwendung insbesondere zur impfung

Info

Publication number
ATE554172T1
ATE554172T1 AT00938875T AT00938875T ATE554172T1 AT E554172 T1 ATE554172 T1 AT E554172T1 AT 00938875 T AT00938875 T AT 00938875T AT 00938875 T AT00938875 T AT 00938875T AT E554172 T1 ATE554172 T1 AT E554172T1
Authority
AT
Austria
Prior art keywords
derivatives
induce
ctl
retro
hiv
Prior art date
Application number
AT00938875T
Other languages
English (en)
Inventor
Jeannine Choppin
Villada Isabelle Bourgault
Jean-Gerard Guillet
Francine Connan
Estelle Ferries
Original Assignee
Assist Publ Hopitaux De Paris
Inst Nat Sante Rech Med
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Assist Publ Hopitaux De Paris, Inst Nat Sante Rech Med filed Critical Assist Publ Hopitaux De Paris
Application granted granted Critical
Publication of ATE554172T1 publication Critical patent/ATE554172T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Genetics & Genomics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Communicable Diseases (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • AIDS & HIV (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT00938875T 1999-06-03 2000-05-31 Proteinfragmente die polyepitope der proteine e6 und e7 von hpv umfassen, deren herstellung und verwendung insbesondere zur impfung ATE554172T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9907012A FR2794370B1 (fr) 1999-06-03 1999-06-03 Fragments proteiques polyepitopiques, leur obtention et leurs utilisations notamment en vaccination
PCT/FR2000/001513 WO2000075336A2 (fr) 1999-06-03 2000-05-31 Fragment proteiques polyepitopiques des proteines e6 et e7 de hpv, leur obtention et leurs utilisations notamment en vaccination

Publications (1)

Publication Number Publication Date
ATE554172T1 true ATE554172T1 (de) 2012-05-15

Family

ID=9546327

Family Applications (2)

Application Number Title Priority Date Filing Date
AT00936995T ATE448244T1 (de) 1999-06-03 2000-05-31 Proteinfragmente die polyepitope des nef proteins von hiv umfassen, deren herstellung und verwendungen insbesondere zur impfung
AT00938875T ATE554172T1 (de) 1999-06-03 2000-05-31 Proteinfragmente die polyepitope der proteine e6 und e7 von hpv umfassen, deren herstellung und verwendung insbesondere zur impfung

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT00936995T ATE448244T1 (de) 1999-06-03 2000-05-31 Proteinfragmente die polyepitope des nef proteins von hiv umfassen, deren herstellung und verwendungen insbesondere zur impfung

Country Status (9)

Country Link
US (3) US6783763B1 (de)
EP (3) EP1181314B8 (de)
AT (2) ATE448244T1 (de)
AU (2) AU5229400A (de)
CA (2) CA2375602A1 (de)
DE (1) DE60043299D1 (de)
ES (2) ES2386803T3 (de)
FR (1) FR2794370B1 (de)
WO (2) WO2000075181A1 (de)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL105554A (en) * 1992-05-05 1999-08-17 Univ Leiden Peptides of human papillomavirus for use in preparations elicit a human T cell response
AU5741001A (en) * 2000-04-28 2001-11-12 Ctl Immunotherapies Corp Epitope synchronization in antigen presenting cells
WO2002070006A2 (en) * 2000-12-08 2002-09-12 Academisch Ziekenhuis Leiden Long peptides of 22-45 amino acid residues that induce and/or enhance antigen specific immune responses
EP1213299A1 (de) * 2000-12-08 2002-06-12 Leids Universitair Medisch Centrum Immunogene epitopen des menschlichen Papillom-Virus und deren Anwendung
EP2363406B1 (de) * 2000-12-08 2019-02-27 Academisch Ziekenhuis Leiden Lange Peptide aus 22-45 Aminosäureresten, die die antigenspezifischen Immunreaktionen induzieren und/oder verbessern
FR2820425B1 (fr) * 2001-02-08 2004-01-02 Commissariat Energie Atomique Melange de peptides issus d'une proteine nef et leurs applications
US6933123B2 (en) * 2001-04-05 2005-08-23 Yao Xiong Hu Peptides from the E2, E6, and E7 proteins of human papilloma viruses 16 and 18 for detecting and/or diagnosing cervical and other human papillomavirus associated cancers
FR2824326B1 (fr) * 2001-05-04 2004-03-19 Commissariat Energie Atomique Melange de peptides issus des proteines e6 et/ou e7 de papillomavirus et leurs applications
ATE466871T1 (de) * 2001-07-13 2010-05-15 Cms Peptides Patent Holding Co Biologisch aktive peptide
CN1210294C (zh) 2001-07-13 2005-07-13 一泰医药研究（深圳）有限公司 序列号21的生物活性肽
EP1806410A3 (de) * 2001-08-23 2007-10-24 Merck & Co., Inc. Floureszente, multiplexe HPV-PCR-Assays unter Verwendung multipler Fluorophore
MXPA04005382A (es) 2001-11-07 2005-02-24 Mannkind Corp Vectores de expresion que codifican epitopes de antigenos asociados al objetivo y metodos para su diseno.
CN100543140C (zh) * 2002-10-17 2009-09-23 生物领先公司 抗hpv疫苗的载体以及该载体转化的微生物
US20090028874A1 (en) * 2003-12-24 2009-01-29 Leiden University Medical Center Synthetic Protein as Tumor-Specific Vaccine
CA2552508A1 (en) * 2003-12-31 2005-09-29 Pharmexa Inc. Inducing cellular immune responses to human papillomavirus using peptide and nucleic acid compositions
US10941353B2 (en) 2004-04-28 2021-03-09 Hydrocarbon Technology & Innovation, Llc Methods and mixing systems for introducing catalyst precursor into heavy oil feedstock
KR101354740B1 (ko) 2004-04-28 2014-01-22 헤드워터스 헤비 오일, 엘엘씨 에뷸레이트 베드 하이드로프로세싱 방법 및 시스템 및기존의 에뷸레이트 베드 시스템을 개량하는 방법
EP1753845B1 (de) * 2004-04-28 2018-01-03 Headwaters Heavy Oil, LLC Festbett-hydroprocessing-verfahren und -systeme und verfahren zur aufrüstung eines bestehenden festbettsystems
AU2005314061B2 (en) 2004-12-08 2010-01-28 Gen-Probe Incorporated Detection of nucleic acids from multiple types of human papillomaviruses
US20070037180A1 (en) * 2005-02-10 2007-02-15 Olle Nilsson Site-specific immunization in order to establish antibodies with specificity for the E7 oncoprotein of high-risk HPV's
US7723472B2 (en) * 2005-02-28 2010-05-25 The Regents Of The University Of California Extracellular matrix binding chimeric proteins and methods of use thereof
EP2049559B1 (de) * 2006-07-28 2012-12-19 The Trustees of the University of Pennsylvania Verbesserte hpv-impfstoffe
US8877206B2 (en) 2007-03-22 2014-11-04 Pds Biotechnology Corporation Stimulation of an immune response by cationic lipids
JP6035503B2 (ja) 2007-05-31 2016-11-30 アカデミシュ ジーケンハウス ライデン ハー．オー．デー．エン． ルムク ワクチンで使用するための、子宮頸部悪性腫瘍に浸潤するｔ細胞によって標的とされるｈｐｖエピトープ
US8652482B2 (en) * 2007-10-03 2014-02-18 The Board Of Trustees Of The University Of Arkansas HPV E6 protein T cell epitopes and uses thereof
WO2009056535A2 (en) * 2007-10-29 2009-05-07 Genimmune N.V. Methods and kits for inducing a ctl response using a prime boost regimen
JP5267969B2 (ja) * 2007-12-04 2013-08-21 学校法人慶應義塾 癌ワクチン
CN102137675A (zh) 2008-04-17 2011-07-27 Pds生物科技公司 通过阳离子脂质的对映体刺激免疫应答
EP2334317B1 (de) * 2008-09-16 2017-06-14 The Research Foundation Of State University Of New York Geklammerte peptide und verfahren zu ihrer zusammensetzung
KR101471043B1 (ko) 2009-01-08 2014-12-09 주식회사 바이오리더스 안정적인 항시적 고발현 자궁경부암 치료백신용 벡터 및 그에 의해 형질전환된 재조합 유산균
US8658176B2 (en) * 2009-06-22 2014-02-25 National Health Research Institutes Lipidated tumor-associated antigens and immunotherapeutic compositions
WO2011017162A2 (en) * 2009-08-03 2011-02-10 The Johns Hopkins University Methods for enhancing antigen-specific immune responses
EP2678442B1 (de) * 2011-02-24 2019-01-16 QIAGEN Gaithersburg, Inc. Materialien und verfahren für den nachweis von hpv-nukleinsäuren
JP6600459B2 (ja) * 2011-09-12 2019-10-30 ピーディーエス バイオテクノロジー コーポレイション 粒子状ワクチン製剤
US9790440B2 (en) 2011-09-23 2017-10-17 Headwaters Technology Innovation Group, Inc. Methods for increasing catalyst concentration in heavy oil and/or coal resid hydrocracker
US10286064B2 (en) 2012-06-15 2019-05-14 Pds Biotechnology Corporation Cationic lipid vaccine compositions and methods of use
US9644157B2 (en) 2012-07-30 2017-05-09 Headwaters Heavy Oil, Llc Methods and systems for upgrading heavy oil using catalytic hydrocracking and thermal coking
AU2013317805B2 (en) 2012-09-21 2018-07-26 Pds Biotechnology Corporation Improved vaccine compositions and methods of use
CN103342738B (zh) 2013-07-24 2015-02-18 广州恒上医药技术有限公司 人乳头瘤病毒e6蛋白可诱发同源蛋白间交叉反应性抗体的精细表位肽
WO2016005789A1 (en) * 2014-07-07 2016-01-14 Institut Pasteur Broad range gene and genotype papillomavirus transcriptome as a biomarker of papillomavirus- associated cancer stages
DK3215187T3 (en) 2014-11-04 2018-12-10 Janssen Vaccines & Prevention Bv THERAPEUTIC HPV16 VACCINES
JP6470872B2 (ja) 2015-08-20 2019-02-13 ヤンセン ファッシンズ アンド プリベンション ベーフェーＪａｎｓｓｅｎ Ｖａｃｃｉｎｅｓ ＆ Ｐｒｅｖｅｎｔｉｏｎ Ｂ．Ｖ． 治療用ｈｐｖ１８ワクチン
US11414607B2 (en) 2015-09-22 2022-08-16 Hydrocarbon Technology & Innovation, Llc Upgraded ebullated bed reactor with increased production rate of converted products
US11414608B2 (en) 2015-09-22 2022-08-16 Hydrocarbon Technology & Innovation, Llc Upgraded ebullated bed reactor used with opportunity feedstocks
US11612652B2 (en) 2015-11-13 2023-03-28 Pds Biotechnology Corporation Lipids as synthetic vectors to enhance antigen processing and presentation ex-vivo in dendritic cell therapy
AU2017259259B2 (en) 2016-05-02 2020-11-19 Bavarian Nordic A/S Therapeutic HPV vaccine combinations
JP2019517522A (ja) * 2016-06-03 2019-06-24 エトゥビクス コーポレーション ヒトパピローマウイルス（ｈｐｖ）関連疾患の処置のための組成物及び方法
US11421164B2 (en) 2016-06-08 2022-08-23 Hydrocarbon Technology & Innovation, Llc Dual catalyst system for ebullated bed upgrading to produce improved quality vacuum residue product
MY189551A (en) 2016-06-20 2022-02-16 Isa Pharmaceuticals B V Formulation of a peptide vaccine
CN108164587A (zh) * 2016-12-07 2018-06-15 中国医学科学院肿瘤医院 新的hpv抗原表位
MX2018002577A (es) 2017-03-02 2018-11-09 Hydrocarbon Tech & Innovation Llc Reactor de lecho en ebullicion mejorado con menos sedimento de ensuciamiento.
US11732203B2 (en) 2017-03-02 2023-08-22 Hydrocarbon Technology & Innovation, Llc Ebullated bed reactor upgraded to produce sediment that causes less equipment fouling
EP3743436A4 (de) * 2018-01-24 2021-10-27 The Council Of The Queensland Institute Of Medical Research Hpv-immuntherapie
CA3057131C (en) 2018-10-17 2024-04-23 Hydrocarbon Technology And Innovation, Llc Upgraded ebullated bed reactor with no recycle buildup of asphaltenes in vacuum bottoms
US12497569B2 (en) 2022-05-26 2025-12-16 Hydrocarbon Technology & Innovation, Llc Method and system for mixing catalyst precursor into heavy oil using a high boiling hydrocarbon diluent
WO2024223299A2 (en) 2023-04-26 2024-10-31 Isa Pharmaceuticals B.V. Methods of treating cancer by administering immunogenic compositions and a pd-1 inhibitor

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4777239A (en) * 1986-07-10 1988-10-11 The Board Of Trustees Of The Leland Stanford Junior University Diagnostic peptides of human papilloma virus
FR2641081A1 (de) * 1988-12-23 1990-06-29 Medgenix Group
IE910909A1 (en) * 1990-03-20 1991-09-25 Behringwerke Ag Seroreactive epitopes of human Papillomavirus (HPV) 16¹proteins
US5932412A (en) * 1990-05-11 1999-08-03 Euro-Diagnostica Ab Synthetic peptides in human papillomaviruses 1, 5, 6, 8, 11, 16, 18, 31, 33 and 56, useful in immunoassay for diagnostic purposes
PT523391E (pt) 1991-07-13 2003-08-29 Dade Behring Marburg Gmbh Utilizacao dos peptidos derivados dos genes hpv-16 e6 e e7 para fins de diagnostico
US6419931B1 (en) * 1991-08-26 2002-07-16 Epimmune Inc. Compositions and methods for eliciting CTL immunity
IL105554A (en) * 1992-05-05 1999-08-17 Univ Leiden Peptides of human papillomavirus for use in preparations elicit a human T cell response
EP0728764A4 (de) * 1993-10-19 1999-01-20 Ajinomoto Kk Peptide das eine immunantwort gegen hiv unduziert und arzneimittel die esenthaelt zur vorbeugung oder behandlung von aids
AUPN015794A0 (en) * 1994-12-20 1995-01-19 Csl Limited Variants of human papilloma virus antigens
JP4108126B2 (ja) * 1996-04-26 2008-06-25 リュクスウニヴェルシテート テ レイデン Ｔ細胞ペプチド・エピトープの選択と産生方法および選択したエピトープを組込むワクチン
AUPO390396A0 (en) * 1996-11-29 1996-12-19 Csl Limited Novel promiscuous T helper cell epitopes
FR2771640B1 (fr) * 1997-12-03 2000-02-11 Inst Nat Sante Rech Med Micelles mixtes de lipopeptides pour l'induction d'une reponse immunitaire et leurs utilisations a des fins therapeutiques

Also Published As

Publication number Publication date
ATE448244T1 (de) 2009-11-15
EP1183368A2 (de) 2002-03-06
EP1183368B1 (de) 2012-04-18
US20080248044A1 (en) 2008-10-09
CA2375602A1 (fr) 2000-12-14
FR2794370A1 (fr) 2000-12-08
EP2267132B1 (de) 2014-07-16
CA2375627A1 (en) 2000-12-14
DE60043299D1 (de) 2009-12-24
US6783763B1 (en) 2004-08-31
EP1181314B1 (de) 2009-11-11
AU5411000A (en) 2000-12-28
AU5229400A (en) 2000-12-28
FR2794370B1 (fr) 2003-10-17
EP1181314A1 (de) 2002-02-27
EP1181314B8 (de) 2010-02-17
US20050033025A1 (en) 2005-02-10
ES2509901T3 (es) 2014-10-20
US7288258B2 (en) 2007-10-30
EP2267132A3 (de) 2011-04-20
WO2000075336A2 (fr) 2000-12-14
WO2000075181A1 (fr) 2000-12-14
WO2000075336A3 (fr) 2001-07-26
ES2386803T3 (es) 2012-08-31
EP2267132A2 (de) 2010-12-29

Similar Documents

Publication Publication Date Title
ATE554172T1 (de) Proteinfragmente die polyepitope der proteine e6 und e7 von hpv umfassen, deren herstellung und verwendung insbesondere zur impfung
FI932678A7 (fi) Alfaviruksiin perustuvia DNA-ilmentämissysteemejä
ATE386800T1 (de) Immunogene chimäre umfassend nucleinsäuresequenzen, die signalsequenzpeptide des endoplasmatischen reticulums und mindestens ein anderes peptid codieren, deren verwendung in impfstoffen und zur behandlung von krankheiten
RU2002111335A (ru) Среда для культивирования клеток без белков и без сыворотки
DE69936784D1 (de) Behandlung von gebärmutterhalskrebs
JP7728755B2 (ja) 細胞を再プログラム化して形質細胞様樹状細胞またはｉ型インターフェロン産生細胞に変換する組成物、その方法および用途
RU2401307C1 (ru) РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pFK2, ОБЕСПЕЧИВАЮЩАЯ СИНТЕЗ РЕКОМБИНАНТНОГО ПЕПТИДА, ЯВЛЯЮЩЕГОСЯ АНАЛОГОМ ФРАГМЕНТА КАППА-КАЗЕИНА ЧЕЛОВЕКА, СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОГО ПЕПТИДА И РЕКОМБИНАНТНЫЙ ПЕПТИД, АНАЛОГ ФРАГМЕНТА КАППА-КАЗЕИНА ЧЕЛОВЕКА, ОБЛАДАЮЩИЙ АПОПТОТИЧЕСКОЙ АКТИВНОСТЬЮ ПО ОТНОШЕНИЮ К РАКОВЫМ КЛЕТКАМ
JPWO2002086133A1 (ja) 培地添加剤および動物細胞培養用培地
ES2561483T3 (es) Antígenos mutantes de GAS57 y anticuerpos de GAS57
ATE169958T1 (de) Herstellung der p1 proteine und impfstoffe gegen gonorrhöe
ATE351904T1 (de) Einem peptid aus antigen muc-1 entsprechende dns zur auslösung einer immunreaktion gegen tumorzellen
JPS61225130A (ja) 鶏卵卵白システインの単離方法およびこれを含有するウイルス感染の処置剤
JPH02502825A (ja) 好中球活性化因子
ATE464320T1 (de) Ntb-a, ein oberflächenmolekül impliziert in der aktivität natürlicher killerzellen (nk)
JPH05501500A (ja) ＴＧＦ―βの生合成構築物
CN101041816B (zh) 人工抗原递呈细胞及其制备方法
CN110078793A (zh) 一种具有抗衰老和修复作用的多肽及其用途
BR9713540A (pt) Procedimento para a expressão do gene do vìrus da dengue
NO874704D0 (no) Minaktivin fremstilt ved rekombinant dna-teknikk.
CN105601726B (zh) Oct4在调控IL-31基因表达中的应用
RU2614124C9 (ru) Оптимизированный ген, кодирующий рекомбинантный белок ипфiii
CN101016540B (zh) C-端含有组氨酸标记的hoxb4重组蛋白质的制造方法及用途
CN109293761A (zh) 一种新型细胞重编程因子的表达与纯化方法
Riding et al. A strategy for purification and peptide sequence analysis of bovine ephemeral fever virus structural proteins
Ivey-Hoyle et al. E2F-2, a novel mammalian transcription factor