ATE457361T1 - Verfahren zur quantitativen und/oder vergleichenden messung von mrna- expressionsniveaus in kleinen biologischen proben - Google Patents
Verfahren zur quantitativen und/oder vergleichenden messung von mrna- expressionsniveaus in kleinen biologischen probenInfo
- Publication number
- ATE457361T1 ATE457361T1 AT05744405T AT05744405T ATE457361T1 AT E457361 T1 ATE457361 T1 AT E457361T1 AT 05744405 T AT05744405 T AT 05744405T AT 05744405 T AT05744405 T AT 05744405T AT E457361 T1 ATE457361 T1 AT E457361T1
- Authority
- AT
- Austria
- Prior art keywords
- sequence
- sample
- quantitative
- specific
- modified cdna
- Prior art date
Links
- 108020004999 messenger RNA Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 230000000052 comparative effect Effects 0.000 title abstract 2
- 239000012472 biological sample Substances 0.000 title 1
- 239000002299 complementary DNA Substances 0.000 abstract 4
- 230000003321 amplification Effects 0.000 abstract 3
- 238000006243 chemical reaction Methods 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 238000010839 reverse transcription Methods 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI20040723A FI20040723A0 (fi) | 2004-05-26 | 2004-05-26 | Menetelmä mRNA:n ilmentymistasojen kvantitatiiviseksi ja/tai suhteelliseksi mittaamiseksi pienissä biologisissa näytteissä |
| PCT/FI2005/050176 WO2005116248A1 (en) | 2004-05-26 | 2005-05-25 | Method for quantitative and/or comparative measurement of mrna expression levels in small biological samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE457361T1 true ATE457361T1 (de) | 2010-02-15 |
Family
ID=32338424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05744405T ATE457361T1 (de) | 2004-05-26 | 2005-05-25 | Verfahren zur quantitativen und/oder vergleichenden messung von mrna- expressionsniveaus in kleinen biologischen proben |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US7838228B2 (https=) |
| EP (1) | EP1749105B1 (https=) |
| JP (1) | JP5258289B2 (https=) |
| AT (1) | ATE457361T1 (https=) |
| AU (1) | AU2005248091B2 (https=) |
| CA (1) | CA2568537C (https=) |
| DE (1) | DE602005019285D1 (https=) |
| DK (1) | DK1749105T3 (https=) |
| ES (1) | ES2341013T3 (https=) |
| FI (1) | FI20040723A0 (https=) |
| WO (1) | WO2005116248A1 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11041216B2 (en) | 2007-10-01 | 2021-06-22 | Longhorn Vaccines And Diagnostics, Llc | Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples |
| US20140087964A1 (en) * | 2012-09-24 | 2014-03-27 | University Of Virginia Patent Foundation | Compositions and methods for detecting aberrant regulation, expression, and levels of hgh |
| EP2749654A1 (en) * | 2012-12-28 | 2014-07-02 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Method of analysis of composition of nucleic acid mixtures |
| WO2017091809A1 (en) * | 2015-11-25 | 2017-06-01 | Longhorn Vaccines And Diagnostics, Llc | Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples |
| US11702702B2 (en) * | 2016-04-15 | 2023-07-18 | Predicine, Inc. | Systems and methods for detecting genetic alterations |
| CN111394455A (zh) * | 2020-03-09 | 2020-07-10 | 皖南医学院 | 一种检测前列腺癌的试剂盒及其制备方法 |
| CN115786463A (zh) * | 2022-12-21 | 2023-03-14 | 深圳大学 | 一种高效去除逆转录体系中残留引物的方法及应用 |
| CN116606867B (zh) * | 2023-04-28 | 2024-01-26 | 北京启辰生生物科技有限公司 | 一种提高新冠全长S蛋白mRNA完整性的制备方法及用途 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5674679A (en) * | 1991-09-27 | 1997-10-07 | Amersham Life Science, Inc. | DNA cycle sequencing |
| US6258543B1 (en) * | 1996-05-02 | 2001-07-10 | Xtrana, Inc. | Quantitation of RNA transcripts using genomic DNA as the internal amplification competitor |
| JPH1057064A (ja) | 1996-08-16 | 1998-03-03 | Rikagaku Kenkyusho | 微量遺伝子産物の特異的増幅方法 |
| WO1998035058A2 (en) * | 1997-02-07 | 1998-08-13 | Ribozyme Pharmaceuticals, Inc. | Improved process for detection and quantification of nucleic acid molecules |
| US20030235884A1 (en) | 1999-06-15 | 2003-12-25 | Cummings Richard D. | Polynucleotides encoding core 1 beta3-galactosyl transferase and methods of use thereof |
| WO2001002603A1 (en) * | 1999-06-30 | 2001-01-11 | Jakob Stenman | Diagnostic method for squamous cell carcinoma |
| JP2001078768A (ja) | 1999-09-16 | 2001-03-27 | Otsuka Pharmaceut Co Ltd | 遺伝子発現の測定法 |
| US6618679B2 (en) * | 2000-01-28 | 2003-09-09 | Althea Technologies, Inc. | Methods for analysis of gene expression |
| KR20020089746A (ko) | 2001-05-24 | 2002-11-30 | 주식회사 커벡스 | 크기변형유도 앵커 시발체, 이를 이용한 알티-피씨알 방법및 이를 이용한 진단 방법 |
| US20040091857A1 (en) | 2001-07-20 | 2004-05-13 | Nallur Girish N. | Gene expression profiling |
| US20030022175A1 (en) | 2001-07-26 | 2003-01-30 | Harn-Jing Terng | Diagnostic assay of genetic mutations by discriminating amplification and hybridization |
| JP2004000039A (ja) * | 2002-05-30 | 2004-01-08 | Inst Of Physical & Chemical Res | 遺伝子多型の検出方法 |
| DE10224200C1 (de) * | 2002-05-31 | 2003-08-21 | Artus Ges Fuer Molekularbiolog | Vermehrung von Ribonukleinsäuren |
| JP2004113136A (ja) * | 2002-09-26 | 2004-04-15 | Yamaguchi Technology Licensing Organization Ltd | 非乳頭状腎細胞がんの感受性と相関するｍｍｐ−１プロモーターのｓｎｐを用いた腎細胞がんの発症リスクを予測する分子レベルでの検査方法 |
| JP2004135509A (ja) * | 2002-10-15 | 2004-05-13 | Hitachi Ltd | 遺伝子発現解析法及び遺伝子発現解析用プローブキット |
| WO2005110596A2 (en) | 2004-05-10 | 2005-11-24 | Gorilla Genomics, Inc. | Methods of quantifying targets in multiple samples |
-
2004
- 2004-05-26 FI FI20040723A patent/FI20040723A0/fi not_active Application Discontinuation
-
2005
- 2005-05-25 JP JP2007513986A patent/JP5258289B2/ja not_active Expired - Lifetime
- 2005-05-25 CA CA2568537A patent/CA2568537C/en not_active Expired - Fee Related
- 2005-05-25 EP EP05744405A patent/EP1749105B1/en not_active Expired - Lifetime
- 2005-05-25 DE DE602005019285T patent/DE602005019285D1/de not_active Expired - Lifetime
- 2005-05-25 WO PCT/FI2005/050176 patent/WO2005116248A1/en not_active Ceased
- 2005-05-25 AU AU2005248091A patent/AU2005248091B2/en not_active Ceased
- 2005-05-25 AT AT05744405T patent/ATE457361T1/de active
- 2005-05-25 ES ES05744405T patent/ES2341013T3/es not_active Expired - Lifetime
- 2005-05-25 US US11/596,543 patent/US7838228B2/en not_active Expired - Fee Related
- 2005-05-25 DK DK05744405.1T patent/DK1749105T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| EP1749105A1 (en) | 2007-02-07 |
| EP1749105B1 (en) | 2010-02-10 |
| ES2341013T3 (es) | 2010-06-14 |
| CA2568537A1 (en) | 2005-12-08 |
| AU2005248091A1 (en) | 2005-12-08 |
| FI20040723A0 (fi) | 2004-05-26 |
| JP5258289B2 (ja) | 2013-08-07 |
| DK1749105T3 (da) | 2010-05-25 |
| US20080286769A1 (en) | 2008-11-20 |
| JP2008500034A (ja) | 2008-01-10 |
| DE602005019285D1 (de) | 2010-03-25 |
| CA2568537C (en) | 2013-04-02 |
| US7838228B2 (en) | 2010-11-23 |
| WO2005116248A1 (en) | 2005-12-08 |
| AU2005248091B2 (en) | 2009-12-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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