DK1749105T3 - Fremgangsmåde til kvantitativ og/eller sammenlignende måling af mRNA-ekspressionsniveauer i små biologiske prøver - Google Patents

Fremgangsmåde til kvantitativ og/eller sammenlignende måling af mRNA-ekspressionsniveauer i små biologiske prøver

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Publication number
DK1749105T3
DK1749105T3 DK05744405.1T DK05744405T DK1749105T3 DK 1749105 T3 DK1749105 T3 DK 1749105T3 DK 05744405 T DK05744405 T DK 05744405T DK 1749105 T3 DK1749105 T3 DK 1749105T3
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DK
Denmark
Prior art keywords
sequence
sample
quantitative
specific
modified cdna
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Application number
DK05744405.1T
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English (en)
Inventor
Analytics Oy Expression
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Analytics Oy Expression
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Publication of DK1749105T3 publication Critical patent/DK1749105T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
DK05744405.1T 2004-05-26 2005-05-25 Fremgangsmåde til kvantitativ og/eller sammenlignende måling af mRNA-ekspressionsniveauer i små biologiske prøver DK1749105T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20040723A FI20040723A0 (fi) 2004-05-26 2004-05-26 Menetelmä mRNA:n ilmentymistasojen kvantitatiiviseksi ja/tai suhteelliseksi mittaamiseksi pienissä biologisissa näytteissä
PCT/FI2005/050176 WO2005116248A1 (en) 2004-05-26 2005-05-25 Method for quantitative and/or comparative measurement of mrna expression levels in small biological samples

Publications (1)

Publication Number Publication Date
DK1749105T3 true DK1749105T3 (da) 2010-05-25

Family

ID=32338424

Family Applications (1)

Application Number Title Priority Date Filing Date
DK05744405.1T DK1749105T3 (da) 2004-05-26 2005-05-25 Fremgangsmåde til kvantitativ og/eller sammenlignende måling af mRNA-ekspressionsniveauer i små biologiske prøver

Country Status (11)

Country Link
US (1) US7838228B2 (da)
EP (1) EP1749105B1 (da)
JP (1) JP5258289B2 (da)
AT (1) ATE457361T1 (da)
AU (1) AU2005248091B2 (da)
CA (1) CA2568537C (da)
DE (1) DE602005019285D1 (da)
DK (1) DK1749105T3 (da)
ES (1) ES2341013T3 (da)
FI (1) FI20040723A0 (da)
WO (1) WO2005116248A1 (da)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
US20140087964A1 (en) * 2012-09-24 2014-03-27 University Of Virginia Patent Foundation Compositions and methods for detecting aberrant regulation, expression, and levels of hgh
EP2749654A1 (en) * 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method of analysis of composition of nucleic acid mixtures
WO2017091809A1 (en) * 2015-11-25 2017-06-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
WO2017181161A1 (en) * 2016-04-15 2017-10-19 Predicine, Inc. Systems and methods for detecting genetic alterations
CN111394455A (zh) * 2020-03-09 2020-07-10 皖南医学院 一种检测前列腺癌的试剂盒及其制备方法
CN116606867B (zh) * 2023-04-28 2024-01-26 北京启辰生生物科技有限公司 一种提高新冠全长S蛋白mRNA完整性的制备方法及用途

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674679A (en) * 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US6258543B1 (en) 1996-05-02 2001-07-10 Xtrana, Inc. Quantitation of RNA transcripts using genomic DNA as the internal amplification competitor
JPH1057064A (ja) * 1996-08-16 1998-03-03 Rikagaku Kenkyusho 微量遺伝子産物の特異的増幅方法
WO1998035058A2 (en) 1997-02-07 1998-08-13 Ribozyme Pharmaceuticals, Inc. Improved process for detection and quantification of nucleic acid molecules
US20030235884A1 (en) * 1999-06-15 2003-12-25 Cummings Richard D. Polynucleotides encoding core 1 beta3-galactosyl transferase and methods of use thereof
WO2001002603A1 (en) 1999-06-30 2001-01-11 Jakob Stenman Diagnostic method for squamous cell carcinoma
JP2001078768A (ja) 1999-09-16 2001-03-27 Otsuka Pharmaceut Co Ltd 遺伝子発現の測定法
AU2001234608A1 (en) * 2000-01-28 2001-08-07 Genetrace Systems, Inc. Methods for analysis of gene expression
KR20020089746A (ko) 2001-05-24 2002-11-30 주식회사 커벡스 크기변형유도 앵커 시발체, 이를 이용한 알티-피씨알 방법및 이를 이용한 진단 방법
US20040091857A1 (en) * 2001-07-20 2004-05-13 Nallur Girish N. Gene expression profiling
US20030022175A1 (en) 2001-07-26 2003-01-30 Harn-Jing Terng Diagnostic assay of genetic mutations by discriminating amplification and hybridization
JP2004000039A (ja) * 2002-05-30 2004-01-08 Inst Of Physical & Chemical Res 遺伝子多型の検出方法
DE10224200C1 (de) * 2002-05-31 2003-08-21 Artus Ges Fuer Molekularbiolog Vermehrung von Ribonukleinsäuren
JP2004113136A (ja) * 2002-09-26 2004-04-15 Yamaguchi Technology Licensing Organization Ltd 非乳頭状腎細胞がんの感受性と相関するｍｍｐ−１プロモーターのｓｎｐを用いた腎細胞がんの発症リスクを予測する分子レベルでの検査方法
JP2004135509A (ja) * 2002-10-15 2004-05-13 Hitachi Ltd 遺伝子発現解析法及び遺伝子発現解析用プローブキット
WO2005110596A2 (en) 2004-05-10 2005-11-24 Gorilla Genomics, Inc. Methods of quantifying targets in multiple samples

Also Published As

Publication number Publication date
DE602005019285D1 (de) 2010-03-25
FI20040723A0 (fi) 2004-05-26
WO2005116248A1 (en) 2005-12-08
CA2568537A1 (en) 2005-12-08
JP5258289B2 (ja) 2013-08-07
US20080286769A1 (en) 2008-11-20
JP2008500034A (ja) 2008-01-10
CA2568537C (en) 2013-04-02
ES2341013T3 (es) 2010-06-14
ATE457361T1 (de) 2010-02-15
AU2005248091B2 (en) 2009-12-17
AU2005248091A1 (en) 2005-12-08
EP1749105A1 (en) 2007-02-07
EP1749105B1 (en) 2010-02-10
US7838228B2 (en) 2010-11-23

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