DK2396430T3 - Template-uafhængig ligering af enkelt-strenget dna - Google Patents

Template-uafhængig ligering af enkelt-strenget dna

Info

Publication number
DK2396430T3
DK2396430T3 DK10741881.6T DK10741881T DK2396430T3 DK 2396430 T3 DK2396430 T3 DK 2396430T3 DK 10741881 T DK10741881 T DK 10741881T DK 2396430 T3 DK2396430 T3 DK 2396430T3
Authority
DK
Denmark
Prior art keywords
methods
reaction mixtures
template
ligation reaction
independent
Prior art date
Application number
DK10741881.6T
Other languages
English (en)
Inventor
Jerome Jendrisak
Joanne Decker
Gary Dahl
Original Assignee
Epict Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42562101&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2396430(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Epict Technologies Corp filed Critical Epict Technologies Corp
Application granted granted Critical
Publication of DK2396430T3 publication Critical patent/DK2396430T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DK10741881.6T 2009-02-16 2010-02-16 Template-uafhængig ligering af enkelt-strenget dna DK2396430T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15286809P 2009-02-16 2009-02-16
PCT/US2010/024319 WO2010094040A1 (en) 2009-02-16 2010-02-16 Template-independent ligation of single-stranded dna

Publications (1)

Publication Number Publication Date
DK2396430T3 true DK2396430T3 (da) 2013-07-15

Family

ID=42562101

Family Applications (1)

Application Number Title Priority Date Filing Date
DK10741881.6T DK2396430T3 (da) 2009-02-16 2010-02-16 Template-uafhængig ligering af enkelt-strenget dna

Country Status (8)

Country Link
EP (2) EP2396430B1 (da)
JP (2) JP2012517813A (da)
CN (1) CN102317475B (da)
AU (1) AU2010213510B2 (da)
CA (1) CA2751470C (da)
DK (1) DK2396430T3 (da)
SG (1) SG173105A1 (da)
WO (1) WO2010094040A1 (da)

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EA021797B1 (ru) 2009-02-13 2015-09-30 Икс-Чем, Инк. Способы создания и скрининга библиотек, кодируемых днк
WO2011112718A1 (en) * 2010-03-10 2011-09-15 Ibis Biosciences, Inc. Production of single-stranded circular nucleic acid
EP2619329B1 (en) 2010-09-24 2019-05-22 The Board of Trustees of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
IN2014DN00221A (da) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
BR112014005205A2 (pt) * 2011-09-07 2017-03-21 X-Chem Inc métodos para etiquetar bibliotecas codificadas com dna
NZ739931A (en) 2012-07-13 2019-08-30 X Chem Inc Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
US20140128292A1 (en) * 2012-09-27 2014-05-08 Bioo Scientific Corporation Methods for improving ligation steps to minimize bias during production of libraries for massively parallel sequencing
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
EP2964779B1 (en) 2013-03-08 2018-08-29 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US10655167B2 (en) 2013-07-26 2020-05-19 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9644232B2 (en) 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9828600B2 (en) 2013-09-20 2017-11-28 University Of Massachusetts Compositions and methods for constructing cDNA libraries that allow for mapping the 5′ and 3′ ends of RNAs
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
JP6435334B2 (ja) 2013-12-11 2018-12-05 アキュラジェン ホールディングス リミテッド 稀な配列変異体を検出するための組成物および方法
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
GB201403216D0 (en) 2014-02-24 2014-04-09 Cambridge Epigenetix Ltd Nucleic acid sample preparation
EP3201350B1 (en) * 2014-09-30 2020-12-02 Global Life Sciences Solutions USA LLC Method for nucleic acid analysis directly from an unpurified biological sample
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
ES2878185T3 (es) 2015-05-28 2021-11-18 Stichting Het Nederlands Kanker Inst Antoni Van Leeuwenhoek Ziekenhuis Ensayos para identificar elementos genéticos que afectan al fenotipo
CN115161178A (zh) 2015-09-09 2022-10-11 集联健康有限公司 用于样品收集、稳定化和保存的系统、方法和装置
CN114807323A (zh) 2015-10-09 2022-07-29 安可济控股有限公司 用于富集扩增产物的方法及组合物
JP2019500852A (ja) * 2015-11-05 2019-01-17 ゼネラル・エレクトリック・カンパニイ リガーゼ支援核酸環状化および増幅
WO2017112666A1 (en) 2015-12-21 2017-06-29 Somagenics, Inc. Methods of library construction for polynucleotide sequencing
EP3414324B1 (en) 2016-02-08 2021-01-20 Rgene, Inc. Multiple ligase compositions, systems, and methods
US11427866B2 (en) 2016-05-16 2022-08-30 Accuragen Holdings Limited Method of improved sequencing by strand identification
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US10590451B2 (en) 2016-07-01 2020-03-17 Personal Genomics, Inc. Methods of constructing a circular template and detecting DNA molecules
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US11091791B2 (en) * 2017-02-24 2021-08-17 Mgi Tech Co., Ltd. Methods for hybridization based hook ligation
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
EP3638810A4 (en) * 2017-06-14 2021-03-24 RealSeq Biosciences, Inc. METHOD OF LIBRARY CONSTRUCTION FOR TARGET POLYNUCLEOTIDES
CN111278974A (zh) 2017-11-09 2020-06-12 深圳华大智造科技有限公司 钩状探针、核酸连接方法以及测序文库的构建方法
CN111386362A (zh) * 2017-11-27 2020-07-07 深圳华大生命科学研究院 一种体液游离dna的文库构建方法及其应用
WO2019149958A1 (en) 2018-02-05 2019-08-08 F. Hoffmann-La Roche Ag Generation of single-stranded circular dna templates for single molecule
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
CN110551794B (zh) * 2018-06-04 2023-05-30 完整基因有限公司 对rna分子进行处理的方法及试剂盒和复合体
CN114364829A (zh) * 2019-06-26 2022-04-15 北京贝瑞和康生物技术有限公司 一种构建PacBio测序文库的方法
CN110724728B (zh) * 2019-10-12 2023-06-06 深圳清华大学研究院 一种环状dna的制备方法
CN115803433A (zh) 2020-07-17 2023-03-14 凯杰贝佛利有限公司 序列偏倚降低的热稳定连接酶
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Also Published As

Publication number Publication date
EP2610352A1 (en) 2013-07-03
EP2610352B1 (en) 2014-10-08
JP2012517813A (ja) 2012-08-09
EP2396430B1 (en) 2013-05-01
WO2010094040A1 (en) 2010-08-19
CA2751470A1 (en) 2010-08-19
AU2010213510B2 (en) 2014-05-01
CN102317475B (zh) 2014-07-16
EP2396430A4 (en) 2012-07-18
AU2010213510A1 (en) 2011-08-18
EP2396430A1 (en) 2011-12-21
CA2751470C (en) 2016-07-26
SG173105A1 (en) 2011-09-29
CN102317475A (zh) 2012-01-11
JP6108494B2 (ja) 2017-04-05
JP2016013127A (ja) 2016-01-28

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