ATE457361T1 - Verfahren zur quantitativen und/oder vergleichenden messung von mrna- expressionsniveaus in kleinen biologischen proben - Google Patents

Verfahren zur quantitativen und/oder vergleichenden messung von mrna- expressionsniveaus in kleinen biologischen proben

Info

Publication number
ATE457361T1
ATE457361T1 AT05744405T AT05744405T ATE457361T1 AT E457361 T1 ATE457361 T1 AT E457361T1 AT 05744405 T AT05744405 T AT 05744405T AT 05744405 T AT05744405 T AT 05744405T AT E457361 T1 ATE457361 T1 AT E457361T1
Authority
AT
Austria
Prior art keywords
sequence
sample
quantitative
specific
modified cdna
Prior art date
Application number
AT05744405T
Other languages
English (en)
Inventor
Jakob Stenman
Annukka Paju
Oso Rissanen
Arto Orpana
Original Assignee
Jakob Stenman
Annukka Paju
Oso Rissanen
Arto Orpana
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jakob Stenman, Annukka Paju, Oso Rissanen, Arto Orpana filed Critical Jakob Stenman
Application granted granted Critical
Publication of ATE457361T1 publication Critical patent/ATE457361T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
AT05744405T 2004-05-26 2005-05-25 Verfahren zur quantitativen und/oder vergleichenden messung von mrna- expressionsniveaus in kleinen biologischen proben ATE457361T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20040723A FI20040723A0 (fi) 2004-05-26 2004-05-26 Menetelmä mRNA:n ilmentymistasojen kvantitatiiviseksi ja/tai suhteelliseksi mittaamiseksi pienissä biologisissa näytteissä
PCT/FI2005/050176 WO2005116248A1 (en) 2004-05-26 2005-05-25 Method for quantitative and/or comparative measurement of mrna expression levels in small biological samples

Publications (1)

Publication Number Publication Date
ATE457361T1 true ATE457361T1 (de) 2010-02-15

Family

ID=32338424

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05744405T ATE457361T1 (de) 2004-05-26 2005-05-25 Verfahren zur quantitativen und/oder vergleichenden messung von mrna- expressionsniveaus in kleinen biologischen proben

Country Status (11)

Country Link
US (1) US7838228B2 (de)
EP (1) EP1749105B1 (de)
JP (1) JP5258289B2 (de)
AT (1) ATE457361T1 (de)
AU (1) AU2005248091B2 (de)
CA (1) CA2568537C (de)
DE (1) DE602005019285D1 (de)
DK (1) DK1749105T3 (de)
ES (1) ES2341013T3 (de)
FI (1) FI20040723A0 (de)
WO (1) WO2005116248A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
US20140087964A1 (en) * 2012-09-24 2014-03-27 University Of Virginia Patent Foundation Compositions and methods for detecting aberrant regulation, expression, and levels of hgh
EP2749654A1 (de) * 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur Analyse der Zusammensetzung von Nukleinsäuremischungen
WO2017091809A1 (en) * 2015-11-25 2017-06-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
WO2017181161A1 (en) * 2016-04-15 2017-10-19 Predicine, Inc. Systems and methods for detecting genetic alterations
CN111394455A (zh) * 2020-03-09 2020-07-10 皖南医学院 一种检测前列腺癌的试剂盒及其制备方法
CN116606867B (zh) * 2023-04-28 2024-01-26 北京启辰生生物科技有限公司 一种提高新冠全长S蛋白mRNA完整性的制备方法及用途

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674679A (en) * 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US6258543B1 (en) 1996-05-02 2001-07-10 Xtrana, Inc. Quantitation of RNA transcripts using genomic DNA as the internal amplification competitor
JPH1057064A (ja) 1996-08-16 1998-03-03 Rikagaku Kenkyusho 微量遺伝子産物の特異的増幅方法
WO1998035058A2 (en) 1997-02-07 1998-08-13 Ribozyme Pharmaceuticals, Inc. Improved process for detection and quantification of nucleic acid molecules
US20030235884A1 (en) 1999-06-15 2003-12-25 Cummings Richard D. Polynucleotides encoding core 1 beta3-galactosyl transferase and methods of use thereof
WO2001002603A1 (en) 1999-06-30 2001-01-11 Jakob Stenman Diagnostic method for squamous cell carcinoma
JP2001078768A (ja) 1999-09-16 2001-03-27 Otsuka Pharmaceut Co Ltd 遺伝子発現の測定法
AU2001234608A1 (en) * 2000-01-28 2001-08-07 Genetrace Systems, Inc. Methods for analysis of gene expression
KR20020089746A (ko) 2001-05-24 2002-11-30 주식회사 커벡스 크기변형유도 앵커 시발체, 이를 이용한 알티-피씨알 방법및 이를 이용한 진단 방법
US20040091857A1 (en) 2001-07-20 2004-05-13 Nallur Girish N. Gene expression profiling
US20030022175A1 (en) 2001-07-26 2003-01-30 Harn-Jing Terng Diagnostic assay of genetic mutations by discriminating amplification and hybridization
JP2004000039A (ja) * 2002-05-30 2004-01-08 Inst Of Physical & Chemical Res 遺伝子多型の検出方法
DE10224200C1 (de) * 2002-05-31 2003-08-21 Artus Ges Fuer Molekularbiolog Vermehrung von Ribonukleinsäuren
JP2004113136A (ja) * 2002-09-26 2004-04-15 Yamaguchi Technology Licensing Organization Ltd 非乳頭状腎細胞がんの感受性と相関するmmp−1プロモーターのsnpを用いた腎細胞がんの発症リスクを予測する分子レベルでの検査方法
JP2004135509A (ja) * 2002-10-15 2004-05-13 Hitachi Ltd 遺伝子発現解析法及び遺伝子発現解析用プローブキット
WO2005110596A2 (en) 2004-05-10 2005-11-24 Gorilla Genomics, Inc. Methods of quantifying targets in multiple samples

Also Published As

Publication number Publication date
CA2568537A1 (en) 2005-12-08
JP2008500034A (ja) 2008-01-10
ES2341013T3 (es) 2010-06-14
AU2005248091B2 (en) 2009-12-17
EP1749105A1 (de) 2007-02-07
WO2005116248A1 (en) 2005-12-08
JP5258289B2 (ja) 2013-08-07
DK1749105T3 (da) 2010-05-25
EP1749105B1 (de) 2010-02-10
AU2005248091A1 (en) 2005-12-08
FI20040723A0 (fi) 2004-05-26
CA2568537C (en) 2013-04-02
US7838228B2 (en) 2010-11-23
DE602005019285D1 (de) 2010-03-25
US20080286769A1 (en) 2008-11-20

Similar Documents

Publication Publication Date Title
ATE457361T1 (de) Verfahren zur quantitativen und/oder vergleichenden messung von mrna- expressionsniveaus in kleinen biologischen proben
JP6009096B2 (ja) 核酸の標準化された配列決定のための方法およびその使用
Soverini et al. Next-generation sequencing for sensitive detection of BCR-ABL1 mutations relevant to tyrosine kinase inhibitor choice in imatinib-resistant patients
US20100159533A1 (en) Simplified sample preparation for rna analysis
Aanes et al. Normalization of RNA-sequencing data from samples with varying mRNA levels
MacRae et al. RNA-Seq reveals spliceosome and proteasome genes as most consistent transcripts in human cancer cells
JP2010209076A5 (de)
ATE546550T1 (de) Verfahren zum nachweis von hpv sowie sonden, primer und kits
EA202192446A1 (ru) Диагностика фетальной хромосомной анэуплоидии с использованием геномного секвенирования
Moustafa et al. Genetic approaches to study plant responses to environmental stresses: an overview
CN104963000A (zh) 一种快速构建单细胞dna测序文库的方法和试剂盒
RU2019109011A (ru) Композиции и способы с использованием фармакогеномного маркера
Dowling et al. The importance of selecting the appropriate reference genes for quantitative real time PCR as illustrated using colon cancer cells and tissue
DE602005017252D1 (de) Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates
Lohmann et al. Gene expression analysis in biomarker research and early drug development using function tested reverse transcription quantitative real-time PCR assays
Holland et al. MPS analysis of the mtDNA hypervariable regions on the MiSeq with improved enrichment
Gumna et al. RNAthor–fast, accurate normalization, visualization and statistical analysis of RNA probing data resolved by capillary electrophoresis
Chang et al. Microarray-driven validation of reference genes for quantitative real-time polymerase chain reaction in a rat vocal fold model of mucosal injury
DE602005027809D1 (de) Primer und sonden zum nachweis von hiv
Demarest et al. Rapid expression profiling of brain microvascular endothelial cells by immuno-laser capture microdissection coupled to TaqMan® low density array
CN107075578B (zh) miRNA表达量的比较分析方法和装置
RU2017132275A (ru) Способ измерения изменения в иммунном репертуаре индивидуума
CN109251964A (zh) 循环microRNAs检测试剂盒和特异性检测循环microRNAs的方法及应用
Wang et al. Absolute quantification of circRNA using digital reverse transcription-hyperbranched rolling circle amplification
Loughman et al. Analytical validation of a novel 6-gene signature for prediction of distant recurrence in estrogen receptor-positive, HER2-negative, early-stage breast cancer

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1749105

Country of ref document: EP