DE602005019285D1 - Verfahren zur quantitativen und/oder vergleichendeiologischen proben - Google Patents

Verfahren zur quantitativen und/oder vergleichendeiologischen proben

Info

Publication number
DE602005019285D1
DE602005019285D1 DE602005019285T DE602005019285T DE602005019285D1 DE 602005019285 D1 DE602005019285 D1 DE 602005019285D1 DE 602005019285 T DE602005019285 T DE 602005019285T DE 602005019285 T DE602005019285 T DE 602005019285T DE 602005019285 D1 DE602005019285 D1 DE 602005019285D1
Authority
DE
Germany
Prior art keywords
sequence
sample
specific
quantitative
modified cdna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602005019285T
Other languages
German (de)
English (en)
Inventor
Jakob Stenman
Annukka Paju
Oso Rissanen
Arto Orpana
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of DE602005019285D1 publication Critical patent/DE602005019285D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
DE602005019285T 2004-05-26 2005-05-25 Verfahren zur quantitativen und/oder vergleichendeiologischen proben Expired - Lifetime DE602005019285D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20040723A FI20040723A0 (fi) 2004-05-26 2004-05-26 Menetelmä mRNA:n ilmentymistasojen kvantitatiiviseksi ja/tai suhteelliseksi mittaamiseksi pienissä biologisissa näytteissä
PCT/FI2005/050176 WO2005116248A1 (en) 2004-05-26 2005-05-25 Method for quantitative and/or comparative measurement of mrna expression levels in small biological samples

Publications (1)

Publication Number Publication Date
DE602005019285D1 true DE602005019285D1 (de) 2010-03-25

Family

ID=32338424

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005019285T Expired - Lifetime DE602005019285D1 (de) 2004-05-26 2005-05-25 Verfahren zur quantitativen und/oder vergleichendeiologischen proben

Country Status (11)

Country Link
US (1) US7838228B2 (https=)
EP (1) EP1749105B1 (https=)
JP (1) JP5258289B2 (https=)
AT (1) ATE457361T1 (https=)
AU (1) AU2005248091B2 (https=)
CA (1) CA2568537C (https=)
DE (1) DE602005019285D1 (https=)
DK (1) DK1749105T3 (https=)
ES (1) ES2341013T3 (https=)
FI (1) FI20040723A0 (https=)
WO (1) WO2005116248A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
US20140087964A1 (en) * 2012-09-24 2014-03-27 University Of Virginia Patent Foundation Compositions and methods for detecting aberrant regulation, expression, and levels of hgh
EP2749654A1 (en) * 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method of analysis of composition of nucleic acid mixtures
WO2017091809A1 (en) * 2015-11-25 2017-06-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
US11702702B2 (en) * 2016-04-15 2023-07-18 Predicine, Inc. Systems and methods for detecting genetic alterations
CN111394455A (zh) * 2020-03-09 2020-07-10 皖南医学院 一种检测前列腺癌的试剂盒及其制备方法
CN115786463A (zh) * 2022-12-21 2023-03-14 深圳大学 一种高效去除逆转录体系中残留引物的方法及应用
CN116606867B (zh) * 2023-04-28 2024-01-26 北京启辰生生物科技有限公司 一种提高新冠全长S蛋白mRNA完整性的制备方法及用途

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674679A (en) * 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US6258543B1 (en) * 1996-05-02 2001-07-10 Xtrana, Inc. Quantitation of RNA transcripts using genomic DNA as the internal amplification competitor
JPH1057064A (ja) 1996-08-16 1998-03-03 Rikagaku Kenkyusho 微量遺伝子産物の特異的増幅方法
WO1998035058A2 (en) * 1997-02-07 1998-08-13 Ribozyme Pharmaceuticals, Inc. Improved process for detection and quantification of nucleic acid molecules
US20030235884A1 (en) 1999-06-15 2003-12-25 Cummings Richard D. Polynucleotides encoding core 1 beta3-galactosyl transferase and methods of use thereof
WO2001002603A1 (en) * 1999-06-30 2001-01-11 Jakob Stenman Diagnostic method for squamous cell carcinoma
JP2001078768A (ja) 1999-09-16 2001-03-27 Otsuka Pharmaceut Co Ltd 遺伝子発現の測定法
US6618679B2 (en) * 2000-01-28 2003-09-09 Althea Technologies, Inc. Methods for analysis of gene expression
KR20020089746A (ko) 2001-05-24 2002-11-30 주식회사 커벡스 크기변형유도 앵커 시발체, 이를 이용한 알티-피씨알 방법및 이를 이용한 진단 방법
US20040091857A1 (en) 2001-07-20 2004-05-13 Nallur Girish N. Gene expression profiling
US20030022175A1 (en) 2001-07-26 2003-01-30 Harn-Jing Terng Diagnostic assay of genetic mutations by discriminating amplification and hybridization
JP2004000039A (ja) * 2002-05-30 2004-01-08 Inst Of Physical & Chemical Res 遺伝子多型の検出方法
DE10224200C1 (de) * 2002-05-31 2003-08-21 Artus Ges Fuer Molekularbiolog Vermehrung von Ribonukleinsäuren
JP2004113136A (ja) * 2002-09-26 2004-04-15 Yamaguchi Technology Licensing Organization Ltd 非乳頭状腎細胞がんの感受性と相関するmmp−1プロモーターのsnpを用いた腎細胞がんの発症リスクを予測する分子レベルでの検査方法
JP2004135509A (ja) * 2002-10-15 2004-05-13 Hitachi Ltd 遺伝子発現解析法及び遺伝子発現解析用プローブキット
WO2005110596A2 (en) 2004-05-10 2005-11-24 Gorilla Genomics, Inc. Methods of quantifying targets in multiple samples

Also Published As

Publication number Publication date
EP1749105A1 (en) 2007-02-07
EP1749105B1 (en) 2010-02-10
ES2341013T3 (es) 2010-06-14
CA2568537A1 (en) 2005-12-08
AU2005248091A1 (en) 2005-12-08
FI20040723A0 (fi) 2004-05-26
JP5258289B2 (ja) 2013-08-07
DK1749105T3 (da) 2010-05-25
US20080286769A1 (en) 2008-11-20
JP2008500034A (ja) 2008-01-10
CA2568537C (en) 2013-04-02
ATE457361T1 (de) 2010-02-15
US7838228B2 (en) 2010-11-23
WO2005116248A1 (en) 2005-12-08
AU2005248091B2 (en) 2009-12-17

Similar Documents

Publication Publication Date Title
Courts et al. Specific micro‐RNA signatures for the detection of saliva and blood in forensic body‐fluid identification
WO2022212711A3 (en) Methods for identification and ratio determination of rna species in multivalent rna compositions
Aanes et al. Normalization of RNA-sequencing data from samples with varying mRNA levels
Soverini et al. Next-generation sequencing for sensitive detection of BCR-ABL1 mutations relevant to tyrosine kinase inhibitor choice in imatinib-resistant patients
WO2009076485A3 (en) Sequencing of nucleic acids
DE60324188D1 (de) Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen
JP2010209076A5 (https=)
US9677130B2 (en) Methods to detect and quantify RNA
WO2009152928A3 (de) Verfahren zur quantitativen analyse von nukleinsäuren, marker dafür und deren verwendung
Moustafa et al. Genetic approaches to study plant responses to environmental stresses: an overview
Okino et al. Evaluation of bias associated with high-multiplex, target-specific pre-amplification
CN110452959B (zh) 一种黄草乌实时定量pcr内参基因的筛选方法
DE602005019285D1 (de) Verfahren zur quantitativen und/oder vergleichendeiologischen proben
Dowling et al. The importance of selecting the appropriate reference genes for quantitative real time PCR as illustrated using colon cancer cells and tissue
RU2019109011A (ru) Композиции и способы с использованием фармакогеномного маркера
Wang et al. Absolute quantification of circRNA using digital reverse transcription-hyperbranched rolling circle amplification
Fang et al. MicroRNA profile analysis for discrimination of monozygotic twins using massively parallel sequencing and real-time PCR
US20150354000A1 (en) Method of analysis of composition of nucleic acid mixtures
Lohmann et al. Gene expression analysis in biomarker research and early drug development using function tested reverse transcription quantitative real-time PCR assays
CN103866007B (zh) 沙漠齿肋赤藓实时荧光定量pcr内参分子的筛选方法
Loewe Combinational usage of next generation sequencing and qPCR for the analysis of tumor samples
Steiner et al. Patterns of histone H3 lysine 27 monomethylation and erythroid cell type-specific gene expression
Elvidge et al. Development and evaluation of real competitive PCR for high-throughput quantitative applications
KR20170052530A (ko) miRNA 발현량의 비교 해석 방법 및 장치
JP2008500034A5 (https=)

Legal Events

Date Code Title Description
8364 No opposition during term of opposition