ATE332398T1 - Einfach markierte oligonukleotidsonden - Google Patents

Einfach markierte oligonukleotidsonden

Info

Publication number
ATE332398T1
ATE332398T1 AT01959722T AT01959722T ATE332398T1 AT E332398 T1 ATE332398 T1 AT E332398T1 AT 01959722 T AT01959722 T AT 01959722T AT 01959722 T AT01959722 T AT 01959722T AT E332398 T1 ATE332398 T1 AT E332398T1
Authority
AT
Austria
Prior art keywords
probes
nucleic acid
methods
labeled oligonucleotide
oligonucleotide probe
Prior art date
Application number
AT01959722T
Other languages
German (de)
English (en)
Inventor
Carl T Wittwer
Andrew O Crockett
Brian E Caplin
Wade Stevenson
Lori A Wagner
Jian Chen
Noriko Kusukawa
Original Assignee
Univ Utah Res Found
Idaho Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Utah Res Found, Idaho Technology Inc filed Critical Univ Utah Res Found
Application granted granted Critical
Publication of ATE332398T1 publication Critical patent/ATE332398T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
AT01959722T 2000-08-11 2001-08-10 Einfach markierte oligonukleotidsonden ATE332398T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22472600P 2000-08-11 2000-08-11
US24061000P 2000-10-16 2000-10-16

Publications (1)

Publication Number Publication Date
ATE332398T1 true ATE332398T1 (de) 2006-07-15

Family

ID=26918967

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01959722T ATE332398T1 (de) 2000-08-11 2001-08-10 Einfach markierte oligonukleotidsonden

Country Status (8)

Country Link
US (1) US6635427B2 (show.php)
EP (1) EP1307592B1 (show.php)
JP (1) JP5213297B2 (show.php)
AT (1) ATE332398T1 (show.php)
CA (1) CA2417986C (show.php)
DE (1) DE60121342T2 (show.php)
ES (1) ES2267803T3 (show.php)
WO (1) WO2002014555A2 (show.php)

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
FI20000333A0 (fi) * 2000-02-16 2000-02-16 Jussi Nurmi Homogeeninen menetelmä polynukleotidin havaitsemiseksi
US7998673B2 (en) 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
DK1278889T3 (da) * 2000-03-29 2006-11-27 Lgc Ltd Hydridiseringsbeacon og fremgangsmåde til hurtig sekvensdetektion og distinktion
GB2403536B (en) 2002-04-23 2006-11-08 Idaho Technology Inc Sample withdrawal and dispensing device
US7785776B2 (en) * 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
US7122384B2 (en) * 2002-11-06 2006-10-17 E. I. Du Pont De Nemours And Company Resonant light scattering microparticle methods
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
CA2507240C (en) 2002-12-06 2011-05-24 F. Hoffmann-La Roche Ag Multiplex assay detection of pathogenic organisms
EP2284278A1 (en) 2003-04-04 2011-02-16 Roche Diagnostics GmbH Improved system for multi color real time PCR
JP4454249B2 (ja) 2003-04-18 2010-04-21 アークレイ株式会社 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
EP1689764B1 (en) * 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Oligonucleotides labeled with a plurality of fluorophores
FR2866349B1 (fr) * 2004-02-12 2008-05-16 Biomerieux Sa Procede de diagnostic/pronostic d'une thrombose
WO2005098036A1 (en) * 2004-04-12 2005-10-20 Postech Foundation Oligonucleotide for detecting target dna or rna
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
EP1841890B1 (en) * 2005-01-03 2013-08-14 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services Primer for nucleic acid detection
DK1681555T3 (da) 2005-01-18 2007-07-02 Hoffmann La Roche Billeddannelse af fluorscenssignaler under anvendelse af telecentrisk excitation og billeddannende optikker
ATE359500T1 (de) 2005-01-18 2007-05-15 Hoffmann La Roche Fluoreszenzabbildung mittels telezentrizität
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
EP1801114B8 (de) 2005-11-23 2009-12-02 Roche Diagnostics GmbH Polynukleotid mit Phosphatmimetikum
JP4713514B2 (ja) * 2006-02-20 2011-06-29 エフ．ホフマン−ラ ロシュ アーゲー 改善された標識試薬
CA2638854C (en) 2006-02-27 2012-05-15 F. Hoffmann-La Roche Ag Pcr hot start by magnesium sequestration
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
WO2008020090A1 (es) * 2006-08-18 2008-02-21 Laboratorios Indas, S.A. Método y kit para diagnosticar el riesgo de desarrollar una enfermedad trombótica venosa
CA2672034C (en) 2006-12-13 2014-02-18 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
US8247171B2 (en) * 2007-02-01 2012-08-21 Abacus Diagnostica Oy Method for detection of presence of target polynucleotide in samples
WO2009011297A1 (ja) * 2007-07-13 2009-01-22 Arkray, Inc. Ｊａｋ２遺伝子の変異検出用プローブおよびその用途
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
EP2297358A2 (en) 2008-05-30 2011-03-23 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of salmonella
US9393566B2 (en) 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
WO2010018245A1 (es) * 2008-08-12 2010-02-18 Biotools Biotechnological & Medical Laboratories, S.A. Método para la detección y/o cuantificación de un ácido nucleico substrato
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US8691504B2 (en) 2009-05-26 2014-04-08 Xiamen University Method for detecting variations in nucleic acid sequences
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
EP2495335B1 (en) * 2009-10-30 2015-04-08 Arkray, Inc. Probe for detecting polymorphism in egfr genes, and usage therefor
JPWO2011062258A1 (ja) * 2009-11-19 2013-04-11 アークレイ株式会社 Ｍｔｈｆｒ遺伝子増幅用プライマーセット、それを含むｍｔｈｆｒ遺伝子増幅用試薬およびその用途
EP2511370B1 (en) * 2009-12-07 2016-09-07 ARKRAY, Inc. PROBE FOR DETECTING POLYMORPHISMS IN THE K-ras GENE, AND USE THEREOF
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
EP2562255A4 (en) 2010-04-21 2013-10-30 Riken NUCLEIC ACID BASIC ANALOG WITH EXTINGUISHING PROPERTIES AND FLUORESCENCE AND APPLICATION THEREOF
JP5940771B2 (ja) 2010-07-12 2016-06-29 アークレイ株式会社 ａｂｌ遺伝子多型の検出用プローブおよびその用途
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
JP2012235778A (ja) * 2011-04-28 2012-12-06 Arkray Inc アセトアルデヒド脱水素酵素２及びアルコール脱水素酵素２の遺伝子変異を同時に検出する方法
US10557170B2 (en) 2011-06-16 2020-02-11 Gendiag.Exe, S.L. Thromboembolic disease markers
JP6144623B2 (ja) * 2011-06-16 2017-06-07 日鉄住金環境株式会社 核酸測定用の核酸プローブ
PT2535424E (pt) 2011-06-16 2015-10-29 Gendiag Exe Sl Snps associados a doença tromboembólica
CN103797367B (zh) * 2011-09-06 2016-05-25 马克斯.普朗克促进科学协会 用于分析生物大分子复合物的方法及其用途
JP2013074888A (ja) * 2011-09-15 2013-04-25 Arkray Inc ＩＬ２８Ｂ（ｒｓ８０９９９１７）とＩＴＰＡ（ｒｓ１１２７３５４）の変異を検出する方法
US20150126438A1 (en) 2012-01-24 2015-05-07 Beth Israel Deaconess Medical Center, Inc. Novel ChREBP Isoforms and Methods Using the Same
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
WO2014116884A1 (en) 2013-01-24 2014-07-31 California Institute Of Technology Chromophore-based characterization and detection methods
JP6583794B2 (ja) 2013-05-20 2019-10-02 バイオベンチャーズ・リミテッド・ライアビリティ・カンパニーＢｉｏＶｅｎｔｕｒｅｓ， ＬＬＣ 多発性骨髄腫のためのｇｅｐ５モデル
GB201319180D0 (en) * 2013-10-30 2013-12-11 Mast Group Ltd Nucleic acid probe
EP3189159A4 (en) 2014-06-26 2018-05-30 University of Florida Research Foundation, Inc. Rapid detection of infectious agents
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
EP3286338B1 (en) 2015-04-24 2023-11-01 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
CA3012815C (en) * 2016-02-09 2021-09-28 Eiken Kagaku Kabushiki Kaisha Method for detecting target nucleic acid and nucleic acid probe used therein
MX2019001186A (es) 2016-07-29 2019-08-29 Juno Therapeutics Inc Metodos para evaluar la presencia o la ausencia de un virus capaz de replicacion.
CN111050545A (zh) 2017-06-29 2020-04-21 朱诺治疗学股份有限公司 评估与免疫疗法相关的毒性的小鼠模型
SG11202000606TA (en) 2017-07-29 2020-02-27 Juno Therapeutics Inc Reagents for expanding cells expressing recombinant receptors
EP3676403A1 (en) 2017-09-01 2020-07-08 Juno Therapeutics, Inc. Gene expression and assessment of risk of developing toxicity following cell therapy
CN107727618B (zh) * 2017-09-04 2019-12-31 江苏大学 生物芯片和特异性切割位点dna序列检测汞离子的方法
US20210254000A1 (en) 2017-11-01 2021-08-19 Juno Therapeutics, Inc. Process for producing a t cell composition
JP2021511802A (ja) 2018-01-31 2021-05-13 ジュノー セラピューティクス インコーポレイテッド 複製可能ウイルスの存在または非存在を評価するための方法および試薬
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
SG11202104183RA (en) 2018-11-06 2021-05-28 Juno Therapeutics Inc Process for producing genetically engineered t cells
KR20220035032A (ko) 2019-04-17 2022-03-21 알파인 이뮨 사이언시즈, 인코포레이티드 변이체 icos 리간드(icosl) 융합 단백질의 방법 및 용도
US20220249637A1 (en) 2019-06-12 2022-08-11 Juno Therapeutics, Inc. Combination therapy of a cell-mediated cytotoxic therapy and an inhibitor of a prosurvival bcl2 family protein
EP4017527A1 (en) 2019-08-22 2022-06-29 Juno Therapeutics, Inc. Combination therapy of a t cell therapy and an enhancer of zeste homolog 2 (ezh2) inhibitor and related methods
JP7697691B2 (ja) * 2019-09-23 2025-06-24 ユニベルシテイト ゲント Ｓｔｒジェノタイピングのためのプローブおよび方法
KR102107589B1 (ko) * 2019-11-20 2020-05-07 주식회사 유진셀 단일 신호 형광 물질을 이용한 실시간 타겟 핵산 검출 및 정량 방법
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
AU2021209068B2 (en) 2020-01-13 2024-09-19 Aspen Neuroscience, Inc. Method of differentiating neural cells and related compositions and methods of use
JP2023526278A (ja) 2020-05-13 2023-06-21 ジュノー セラピューティクス インコーポレイテッド 組み換え受容体を発現しているドナーバッチ細胞を産生するための方法
US20240216508A1 (en) 2020-06-26 2024-07-04 Juno Therapeutics Gmbh Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
WO2023004371A1 (en) 2021-07-21 2023-01-26 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
CA3241326A1 (en) 2021-12-03 2023-06-08 Medicinal Genomics Corporation Psilocybe assay
IL316055A (en) 2022-04-15 2024-11-01 Aspen Neuroscience Inc Methods of classifying the differentiation state of cells and related compositions of differentiated cells
WO2023230581A1 (en) 2022-05-25 2023-11-30 Celgene Corporation Methods of manufacturing t cell therapies
WO2024059493A1 (en) 2022-09-13 2024-03-21 Medicinal Genomics Corporation Psilocybe assay
WO2025245399A1 (en) 2024-05-23 2025-11-27 Indapta Therapeutics, Inc. Method of treating cancer with natural killer cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
AU693023B2 (en) 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
GB9602025D0 (en) * 1996-02-01 1996-04-03 Amersham Int Plc Nucleoside analogues
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
WO1997043451A1 (en) 1996-05-15 1997-11-20 Biogenex Laboratories Non-nucleotide linking reagents
PT1179600E (pt) 1996-06-04 2005-08-31 Univ Utah Res Found Monitorizacao da hibridacao durante a rcp
US5691145A (en) 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
WO1998026093A2 (en) 1996-12-13 1998-06-18 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
SE522077C2 (sv) 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
JP2001514859A (ja) * 1997-09-04 2001-09-18 バイエル コーポレイション クエンチ可能な蛍光標識を有するオリゴヌクレオチドプローブおよびその使用方法
DE19858588B4 (de) 1998-08-22 2016-04-07 Qiagen Gmbh Farbstoffmarkiertes Oligonukleotid zum Markieren eines Nukleinsäuremoleküls
EP1046717B1 (en) 1999-04-20 2010-10-06 National Institute of Advanced Industrial Science and Technology Method and probes for determining a concentration of target nucleic acid molecules and method for analyzing data obtained by the method
SE9902565D0 (sv) * 1999-07-05 1999-07-05 Jonas Karlsson Probe for analysis of nucleic acids
DK1278889T3 (da) 2000-03-29 2006-11-27 Lgc Ltd Hydridiseringsbeacon og fremgangsmåde til hurtig sekvensdetektion og distinktion

Also Published As

Publication number Publication date
DE60121342T2 (de) 2007-06-28
WO2002014555A2 (en) 2002-02-21
JP2004506431A (ja) 2004-03-04
JP5213297B2 (ja) 2013-06-19
CA2417986C (en) 2013-11-26
CA2417986A1 (en) 2002-02-21
US6635427B2 (en) 2003-10-21
EP1307592A2 (en) 2003-05-07
DE60121342D1 (de) 2006-08-17
EP1307592B1 (en) 2006-07-05
WO2002014555A3 (en) 2003-02-27
ES2267803T3 (es) 2007-03-16
US20030022177A1 (en) 2003-01-30

Similar Documents

Publication Publication Date Title
ATE332398T1 (de) Einfach markierte oligonukleotidsonden
Knemeyer et al. Probes for detection of specific DNA sequences at the single-molecule level
ATE534748T1 (de) Direkter snp-nachweis mit nichtamplifizierter dna
ATE443777T1 (de) Methoden und zusammensetzungen zur analyse von ribonukleinsäuren
WO2003008622A3 (en) Exponential nucleic acid amplification using nicking endonucleases
ATE366821T1 (de) Automatisierte echtzeit-analyse von nukleinsäureamplifikation
JP2004506431A5 (show.php)
WO2003087297A3 (en) Infectious disease microarray
EP1295941A4 (en) NEW NUCLEIC ACID ESTERS AND METHOD FOR TESTING NUCLEIC ACIDS UNDER USE
DE60136567D1 (de) Polynukleotid-proben zum nachweis und zur quantifizierung von candida spezies
ATE368125T1 (de) Verfahren zur erkennung von einzel-nukleotid- polymorphismen
ATE398187T1 (de) Verfahren zum nachweis von ziel-nukleinsäuren mittels pcr
PT1633888E (pt) Meios e método para a detecção de sequências de nucleótidos alvo utilizando ensaios de ligação com pares de sondas de oligonucleótido melhoradas
ATE474933T1 (de) Verfahren zum nachweis von pathogenen organismen
WO2005047468A3 (en) Improved methods for detecting and measuring specific nucleic acid sequences
WO2000068421A3 (de) Verfahren zum nachweisen von mikroorganismen in einer probe
ATE429513T1 (de) Nachweissystem
ATE197071T1 (de) Nachweis von nukleinsäure-amplifikation mittels fluoreszenz-polarisation
ATE445715T1 (de) Elektrokatalytischer nukleinsäurehybridisierungsnachweis
ATE449194T1 (de) Oligonukleotide zur detektion von hepatitis b viren
ATE455864T1 (de) Verfahren zum nachweis von in einer polymerase- kettenreaktion amplifizierten nukleinsäuremolekülen
DE60119902D1 (de) Verfahren für die gleichzeitige und mehrfache detektion und quantifizierung der hybridisierung von molekülverbindungen wie nukleinsäuren, dns, rns, pns und proteinen
CY1105861T1 (el) Μεθοδος για την ανιχνευση δευτepευοντων γονιδιωματων σε τυπους ιου χρησιμοποιωντας μικροτσιπ dna
ATE455867T1 (de) Echtzeit-pcr unter zusatz von pyrophosphatase
ATE491041T1 (de) Verfahren zur quantifizierung von methylierter dns

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties