ATE310016T1 - Fusionsprotein regulatorischer/akzessorischer hiv-proteine - Google Patents

Fusionsprotein regulatorischer/akzessorischer hiv-proteine

Info

Publication number
ATE310016T1
ATE310016T1 AT03730029T AT03730029T ATE310016T1 AT E310016 T1 ATE310016 T1 AT E310016T1 AT 03730029 T AT03730029 T AT 03730029T AT 03730029 T AT03730029 T AT 03730029T AT E310016 T1 ATE310016 T1 AT E310016T1
Authority
AT
Austria
Prior art keywords
proteins
fusion protein
nucleic acids
regulatory
hiv proteins
Prior art date
Application number
AT03730029T
Other languages
English (en)
Inventor
Paul Howley
Sonja Leyrer
Eva Felder
Original Assignee
Bavarian Nordic As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bavarian Nordic As filed Critical Bavarian Nordic As
Application granted granted Critical
Publication of ATE310016T1 publication Critical patent/ATE310016T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • A61K39/21Retroviridae, e.g. equine infectious anemia virus
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/14Antivirals for RNA viruses
    • A61P31/18Antivirals for RNA viruses for HIV
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • A61P37/04Immunostimulants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08RNA viruses
    • C07K14/15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16HIV-1 ; HIV-2
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/51Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525Virus
    • A61K2039/5256Virus expressing foreign proteins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/51Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53DNA (RNA) vaccination
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/24011Poxviridae
    • C12N2710/24111Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16311Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16311Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16334Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Communicable Diseases (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT03730029T 2002-05-16 2003-05-14 Fusionsprotein regulatorischer/akzessorischer hiv-proteine ATE310016T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200200754 2002-05-16
PCT/EP2003/005039 WO2003097675A1 (en) 2002-05-16 2003-05-14 Fusion protein of hiv regulatory/accessory proteins

Publications (1)

Publication Number Publication Date
ATE310016T1 true ATE310016T1 (de) 2005-12-15

Family

ID=29433046

Family Applications (2)

Application Number Title Priority Date Filing Date
AT03730029T ATE310016T1 (de) 2002-05-16 2003-05-14 Fusionsprotein regulatorischer/akzessorischer hiv-proteine
AT05017695T ATE407143T1 (de) 2002-05-16 2003-05-14 Fusionsprotein des regulatorische/akzessorische hiv proteine

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT05017695T ATE407143T1 (de) 2002-05-16 2003-05-14 Fusionsprotein des regulatorische/akzessorische hiv proteine

Country Status (24)

Country Link
US (7) US20050222388A1 (de)
EP (2) EP1506223B1 (de)
JP (3) JP2006502704A (de)
KR (4) KR101196178B1 (de)
CN (3) CN101108882A (de)
AT (2) ATE310016T1 (de)
AU (2) AU2003240638B2 (de)
BR (1) BR0309963A (de)
CA (1) CA2483640A1 (de)
CY (1) CY1108638T1 (de)
DE (2) DE60302356T2 (de)
DK (2) DK1652857T3 (de)
EA (1) EA007154B1 (de)
ES (2) ES2313166T3 (de)
HK (1) HK1076822A1 (de)
IL (3) IL164178A0 (de)
MX (1) MXPA04010683A (de)
NO (1) NO20045229L (de)
NZ (2) NZ536499A (de)
PL (2) PL397252A1 (de)
PT (1) PT1652857E (de)
SI (2) SI1506223T1 (de)
UA (3) UA92788C2 (de)
WO (1) WO2003097675A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7501127B2 (en) 2002-05-16 2009-03-10 Bavarian Nordic A/S Intergenic regions as novel sites for insertion of HIV DNA sequences in the genome of Modified Vaccinia virus Ankara
NZ536499A (en) 2002-05-16 2008-04-30 Bavarian Nordic As Fusion protein comprising the amino acid sequence of at least four different HIV proteins selected from Vif, Vpr, Vpu, Vpx, Rev, Tat and Nef, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini
ATE520708T1 (de) 2003-03-28 2011-09-15 Us Gov Health & Human Serv Immunogenetische hiv-1 multi-klade, multivalente konstrukte und deren verwendung
IL286053B2 (en) * 2005-10-18 2023-03-01 Nat Jewish Health A process for making red blood cells using immortal hematopoietic stem cells and erythropoietin
AU2007345768B2 (en) * 2006-07-27 2013-08-01 Ligocyte Pharmaceuticals, Inc. Chimeric influenza virus-like particles
EP2044224A4 (de) * 2006-07-27 2011-04-13 Ligocyte Pharmaceuticals Inc Chimärische virusähnliche partikel
KR20170078862A (ko) 2008-05-16 2017-07-07 타이가 바이오테크놀로지스, 인코포레이티드 항체 및 그 제조 방법
CA2731767C (en) 2008-07-21 2016-12-06 Taiga Biotechnologies, Inc. Differentiated anucleated cells and method for preparing the same
EP3916010A1 (de) 2008-08-28 2021-12-01 Taiga Biotechnologies, Inc. Modulatoren von myc, verfahren zur verwendung davon und verfahren zur identifizierung von mitteln zur modulierung von myc
CA2767924A1 (en) * 2009-10-08 2011-04-14 Bavarian Nordic A/S Generation of a broad t-cell response in humans against hiv
US9358281B2 (en) * 2009-10-09 2016-06-07 New York University Methods, agents and peptides for inducing an innate immune response in HIV vaccination
CN102753582A (zh) 2009-11-03 2012-10-24 莱戈赛特医药股份有限公司 嵌合RSV-F多肽、和慢病毒或α-反转录病毒GAG基VLP
EP2620446A1 (de) 2012-01-27 2013-07-31 Laboratorios Del Dr. Esteve, S.A. Immunogene zur HIV-Impfung
EP3868387A1 (de) 2012-07-20 2021-08-25 Taiga Biotechnologies, Inc. Verbesserte wiederherstellung und autorenkonstitution des hämatopoietischen abteils
US9365825B2 (en) 2013-03-11 2016-06-14 Taiga Biotechnologies, Inc. Expansion of adult stem cells in vitro
US10272115B2 (en) 2013-03-11 2019-04-30 Taiga Biotechnologies, Inc. Production and use of red blood cells
WO2015192339A1 (zh) * 2014-06-18 2015-12-23 中山大学 一种嵌合载体及其制备方法和应用
CN104130331B (zh) * 2014-06-19 2017-06-06 中山大学 一种抗hiv‑1病毒药物及其制备和应用
FR3042121A1 (fr) 2015-10-08 2017-04-14 Jean-Marc Limacher Composition anti-tumorale
CN105330729A (zh) * 2015-11-18 2016-02-17 南京医科大学 一种人类免疫缺陷病毒Vpr蛋白的制备方法
CN113786476A (zh) 2016-12-02 2021-12-14 泰加生物工艺学公司 纳米颗粒调配物
US10149898B2 (en) 2017-08-03 2018-12-11 Taiga Biotechnologies, Inc. Methods and compositions for the treatment of melanoma
CN116322757A (zh) 2019-11-14 2023-06-23 埃利克斯疗法公司 用于疫苗的剂量方案

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0942747A1 (de) * 1996-08-26 1999-09-22 Chiron Corporation Impfungstherapie nach einer hiv-infektion
GB9720585D0 (en) 1997-09-26 1997-11-26 Smithkline Beecham Biolog Vaccine
IT1297090B1 (it) 1997-12-01 1999-08-03 Barbara Ensoli Tat di hiv-1 o suoi derivati, da soli od in combinazione, a scopo vaccinale, profilattico e terapeutico, contro l'aids i tumori e le
FR2773156B1 (fr) * 1997-12-26 2000-03-31 Biovacs Inc Nouveaux immunogenes anti-retroviraux (toxoides), nouveaux procedes de preparation et application a la prevention et au traitement du sida
FI105105B (fi) 1998-02-27 2000-06-15 Finnish Immunotechnology Ltd O Itsereplikoiva DNA-vektori immunisoimiseksi HIV:ta vastaan
US7935805B1 (en) * 1998-12-31 2011-05-03 Novartis Vaccines & Diagnostics, Inc Polynucleotides encoding antigenic HIV Type C polypeptides, polypeptides and uses thereof
CN1326873C (zh) 2000-01-31 2007-07-18 史密丝克莱恩比彻姆生物有限公司 用于hiv预防或治疗性免疫的疫苗
WO2001092470A2 (en) * 2000-03-02 2001-12-06 Emory University Dna expression vectors and methods of use
US6544780B1 (en) * 2000-06-02 2003-04-08 Genphar, Inc. Adenovirus vector with multiple expression cassettes
AU8349301A (en) * 2000-07-14 2002-01-30 Univ Pennsylvania Dna vaccines encoding hiv accessory proteins
AU2872202A (en) * 2000-11-01 2002-05-15 Us Gov Health & Human Serv Expression vectors able to elicit improved immune response and methods of using same
US7628980B2 (en) * 2000-11-23 2009-12-08 Bavarian Nordic A/S Modified vaccinia virus ankara for the vaccination of neonates
NZ536499A (en) * 2002-05-16 2008-04-30 Bavarian Nordic As Fusion protein comprising the amino acid sequence of at least four different HIV proteins selected from Vif, Vpr, Vpu, Vpx, Rev, Tat and Nef, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini

Also Published As

Publication number Publication date
JP2009178165A (ja) 2009-08-13
EA007154B1 (ru) 2006-08-25
KR20120106894A (ko) 2012-09-26
PT1652857E (pt) 2008-12-12
EP1652857B1 (de) 2008-09-03
WO2003097675A1 (en) 2003-11-27
AU2008252074A1 (en) 2009-01-08
DE60323407D1 (de) 2008-10-16
IL164178A0 (en) 2005-12-18
NO20045229L (no) 2004-11-29
DK1652857T3 (da) 2009-01-05
CN101108882A (zh) 2008-01-23
PL372092A1 (en) 2005-07-11
CN1653085A (zh) 2005-08-10
EP1506223B1 (de) 2005-11-16
SI1506223T1 (sl) 2006-04-30
KR20110099058A (ko) 2011-09-05
SI1652857T1 (sl) 2009-02-28
CA2483640A1 (en) 2003-11-27
NZ556144A (en) 2008-04-30
CN1325512C (zh) 2007-07-11
ES2313166T3 (es) 2009-03-01
CY1108638T1 (el) 2014-04-09
US8143054B2 (en) 2012-03-27
US20100047871A1 (en) 2010-02-25
KR20100063126A (ko) 2010-06-10
US20120142096A1 (en) 2012-06-07
US8435535B2 (en) 2013-05-07
AU2003240638A1 (en) 2003-12-02
CN1982332A (zh) 2007-06-20
EP1652857A2 (de) 2006-05-03
ES2253675T3 (es) 2006-06-01
US20050222388A1 (en) 2005-10-06
US20100047268A1 (en) 2010-02-25
KR101196178B1 (ko) 2012-11-01
KR100996330B1 (ko) 2010-11-23
ATE407143T1 (de) 2008-09-15
US8389275B2 (en) 2013-03-05
EP1506223A1 (de) 2005-02-16
US20060257974A1 (en) 2006-11-16
US20090280561A1 (en) 2009-11-12
PL397252A1 (pl) 2012-04-10
DE60302356D1 (de) 2005-12-22
EP1652857A3 (de) 2007-03-14
JP2006502704A (ja) 2006-01-26
IL164178A (en) 2011-01-31
NZ536499A (en) 2008-04-30
BR0309963A (pt) 2005-02-22
HK1076822A1 (en) 2006-01-27
EA200401504A1 (ru) 2005-04-28
US8147839B2 (en) 2012-04-03
IL197304A0 (en) 2011-07-31
MXPA04010683A (es) 2005-02-17
AU2008252074B2 (en) 2012-02-16
UA82998C2 (ru) 2008-06-10
UA85373C2 (ru) 2009-01-26
UA92788C2 (ru) 2010-12-10
JP2010099084A (ja) 2010-05-06
KR20050010000A (ko) 2005-01-26
US8137931B2 (en) 2012-03-20
EP1652857B9 (de) 2009-10-14
US7569228B2 (en) 2009-08-04
US20120142097A1 (en) 2012-06-07
AU2003240638B2 (en) 2008-05-15
DK1506223T3 (da) 2006-04-10
DE60302356T2 (de) 2006-08-03

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