ATE301835T1 - Eiweisbruchstückergänzungstests zum nachweis biomolekularinteraktionen - Google Patents

Eiweisbruchstückergänzungstests zum nachweis biomolekularinteraktionen

Info

Publication number
ATE301835T1
ATE301835T1 AT98901905T AT98901905T ATE301835T1 AT E301835 T1 ATE301835 T1 AT E301835T1 AT 98901905 T AT98901905 T AT 98901905T AT 98901905 T AT98901905 T AT 98901905T AT E301835 T1 ATE301835 T1 AT E301835T1
Authority
AT
Austria
Prior art keywords
dhfr
fragments
leucine
zipper
coli
Prior art date
Application number
AT98901905T
Other languages
English (en)
Inventor
Stephen William Watso Michnick
Joelle Nina Apartmen Pelletier
Ingrid Remy
Original Assignee
Odyssey Thera Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Odyssey Thera Inc filed Critical Odyssey Thera Inc
Application granted granted Critical
Publication of ATE301835T1 publication Critical patent/ATE301835T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT98901905T 1997-01-31 1998-02-02 Eiweisbruchstückergänzungstests zum nachweis biomolekularinteraktionen ATE301835T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA002196496A CA2196496A1 (en) 1997-01-31 1997-01-31 Protein fragment complementation assay for the detection of protein-protein interactions
PCT/CA1998/000068 WO1998034120A1 (en) 1997-01-31 1998-02-02 Protein fragment complementation assays to detect biomolecular interactions

Publications (1)

Publication Number Publication Date
ATE301835T1 true ATE301835T1 (de) 2005-08-15

Family

ID=4159802

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98901905T ATE301835T1 (de) 1997-01-31 1998-02-02 Eiweisbruchstückergänzungstests zum nachweis biomolekularinteraktionen

Country Status (10)

Country Link
US (7) US6270964B1 (de)
EP (2) EP1605042A3 (de)
JP (1) JP4262778B2 (de)
AT (1) ATE301835T1 (de)
AU (1) AU5850598A (de)
CA (1) CA2196496A1 (de)
DE (1) DE69831136T2 (de)
DK (1) DK0966685T3 (de)
ES (1) ES2247673T3 (de)
WO (1) WO1998034120A1 (de)

Families Citing this family (207)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6897017B1 (en) * 1997-01-31 2005-05-24 Odyssey Thera Inc. Vivo library-versus-library selection of optimized protein-protein interactions
US20020064769A1 (en) * 2000-10-05 2002-05-30 Watson Michnick Stephen William Dynamic visualization of expressed gene networks in living cells
CA2196496A1 (en) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US7062219B2 (en) * 1997-01-31 2006-06-13 Odyssey Thera Inc. Protein fragment complementation assays for high-throughput and high-content screening
US7306914B2 (en) * 1997-01-31 2007-12-11 Odyssey Thera Inc. Protein fragment complementation assays in whole animals applications to drug efficacy, ADME, cancer biology, immunology, infectious disease and gene therapy
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
DK1801214T3 (da) 1997-07-07 2011-01-24 Medical Res Council In vitro sorteringsfremgangsmåde
US6333154B1 (en) * 1997-12-04 2001-12-25 Institut Pasteur Bacterial multi-hybrid system and applications thereof
US20050221280A1 (en) * 1998-02-02 2005-10-06 Odyssey Thera, Inc. Protein-protein interactions for pharmacological profiling
US6828099B2 (en) * 1998-02-02 2004-12-07 Odyssey Thera Inc. Protein fragment complementation assay (PCA) for the detection of protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. coli TEM-1 β-Lactamase
US7855167B2 (en) * 1998-02-02 2010-12-21 Odyssey Thera, Inc. In vivo screening of protein-protein interactions with protein-fragment complementation assays
US7166424B2 (en) * 1998-02-02 2007-01-23 Odyssey Thera Inc. Fragments of fluorescent proteins for protein fragment complementation assays
AU756617B2 (en) 1998-07-28 2003-01-16 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Leptin-mediated gene-induction
AU5143099A (en) * 1998-07-30 2000-02-21 Universite De Montreal Protein fragment complementation assays for the detection of biological or drug interactions
JP2002526756A (ja) * 1998-09-24 2002-08-20 デューク ユニバーシティ 生細胞におけるタンパク質−タンパク質相互作用の測定方法
EP1117762A4 (de) 1998-09-29 2004-02-25 Gamida Cell Ltd Verfahren zur kontrolle der proliferation und differenzierung von stammzellen und vorläuferzellen
US20040038317A1 (en) * 1999-03-15 2004-02-26 Kalobios, Inc. Breakpoint fusion fragment complementation system
US8148110B2 (en) 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
DE60036942T2 (de) * 1999-05-24 2008-08-07 New England Biolabs, Inc., Beverly Verfahren zur herstellung von getrennten, nicht-übertragbaren genen, welche befähigt sind ein aktives proteinprodukt zu exprimieren
US20040096938A1 (en) * 1999-05-24 2004-05-20 Ming-Qun Xu Method for generating split, non-transferable genes that are able to express an active protein product
AU6045900A (en) * 1999-05-25 2000-12-12 Panorama Research, Inc. Interaction-activated proteins
US6673554B1 (en) * 1999-06-14 2004-01-06 Trellie Bioinformatics, Inc. Protein localization assays for toxicity and antidotes thereto
EP1194539A2 (de) * 1999-06-25 2002-04-10 Universität Zürich Hetero-assoziierende coiled-coil peptide und screenings-verfahren dafür
WO2001000866A1 (en) 1999-06-26 2001-01-04 Odyssey Pharmaceuticals, Inc. An in vivo library-versus-library selection of optimized protein-protein interactions
WO2001001137A1 (en) * 1999-06-30 2001-01-04 Children's Medical Center Corporation Fusion protein and uses thereof
AU6363900A (en) * 1999-07-22 2001-02-13 Children's Medical Center Corporation Expression cloning using a tagged cdna library
WO2001051629A2 (en) * 2000-01-13 2001-07-19 Panorama Research, Inc. Circularly permutated, interaction-activated proteins
WO2001053479A2 (en) * 2000-01-24 2001-07-26 Sangamo Biosciences, Inc. Molecular switches ii
WO2001060840A2 (en) * 2000-02-14 2001-08-23 Board Of Regents, The University Of Texas System Complementation assay assessing protein solubility and folding
US6727070B2 (en) 2000-02-14 2004-04-27 The Board Of Regents, The University Of Texas System Protein/solubility folding assessed by structural complementation
US6780599B2 (en) 2000-05-12 2004-08-24 Yale University Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins
WO2001088168A2 (en) * 2000-05-12 2001-11-22 Odyssey Pharmaceuticals, Inc. Mapping molecular interactions in plants with protein fragments complementation assays
DE60112282T2 (de) * 2000-05-22 2006-04-20 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw. Auf rezeptorgrundlage arbeitende screening-verfahren für protein wechselwirkungen
WO2002020565A2 (en) 2000-09-08 2002-03-14 Universität Zürich Collections of repeat proteins comprising repeat modules
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
DE60239097D1 (de) * 2001-03-02 2011-03-17 Gpc Biotech Ag Drei-hybrid-assaysystem
DE10110449A1 (de) * 2001-03-05 2002-09-19 Lisa Wiesmueller Testsystem zur Bestimmung von Genotoxizitäten
US20020177217A1 (en) * 2001-03-26 2002-11-28 Massachusetts Institute Of Technology Method for studying protein-protein interactions
EP1392870B1 (de) * 2001-05-09 2008-02-27 Discoverx, Inc. Screening auf enzyminhibitoren
DE60234131D1 (de) * 2001-08-01 2009-12-03 Cellomics Inc Neue fusionsproteine und molekulare bindungsassays
GB0118995D0 (en) * 2001-08-03 2001-09-26 Univ Wales Medicine Detection of mutations in nucleic acids
AU2007200686B2 (en) * 2001-09-20 2011-07-14 Immunex Corporation Selection of cells expressing heteromeric polypeptides
WO2003035887A1 (en) * 2001-09-20 2003-05-01 Immunex Corporation Selection of cells expressing heteromeric polypeptides
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
WO2003046176A2 (en) 2001-11-26 2003-06-05 Hybrigenics Protein-protein interactions in human immunodeficiency virus
IL152904A0 (en) * 2002-01-24 2003-06-24 Gamida Cell Ltd Utilization of retinoid and vitamin d receptor antagonists for expansion of renewable stem cell populations
EP1465982A4 (de) * 2002-01-25 2006-06-07 Gamida Cell Ltd Verfahren zur expansion von stamm- und vorläuferzellen und damit erhaltene expandierte zellpopulationen
US6936427B2 (en) * 2002-02-08 2005-08-30 Trellis Bioscience, Inc. Real time detection of intermolecular interaction
US7335478B2 (en) * 2002-04-18 2008-02-26 Kalobios Pharmaceuticals, Inc. Reactivation-based molecular interaction sensors
US7432063B2 (en) * 2002-02-14 2008-10-07 Kalobios Pharmaceuticals, Inc. Methods for affinity maturation
SE0200531D0 (sv) * 2002-02-22 2002-02-22 Avaris Ab Novel biomolecular complexes, method for their production and use
ATE377644T1 (de) * 2002-03-25 2007-11-15 Applera Corp Systeme und verfahren zum nachweis der funktion nukleärer rezeptoren unter verwendung von reporterenzymmutanten komplementierungen
WO2003093793A2 (en) * 2002-05-01 2003-11-13 Trellis Bioscience, Inc. Binary or polynary targeting and uses thereof
GB0214553D0 (en) * 2002-06-25 2002-08-07 Univ Leeds Reporter module molecules having active ion channel activity
US7662554B2 (en) * 2002-10-09 2010-02-16 The Trustees Of Boston University Nucleic acid supported protein complementation
US9740817B1 (en) 2002-10-18 2017-08-22 Dennis Sunga Fernandez Apparatus for biological sensing and alerting of pharmaco-genomic mutation
EP1590477B1 (de) 2003-01-29 2009-07-29 454 Corporation Verfahren zur amplifikation und sequenzierung von nukleinsäuren
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
WO2005001115A2 (en) * 2003-05-30 2005-01-06 Odyssey Thera, Inc. Monitoring gene silencing and annotating gene function in living cells
US8048627B2 (en) 2003-07-05 2011-11-01 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
US8346482B2 (en) 2003-08-22 2013-01-01 Fernandez Dennis S Integrated biosensor and simulation system for diagnosis and therapy
CA2541765A1 (en) 2003-10-10 2004-10-01 Promega Corporation Luciferase biosensor
GB0329353D0 (en) * 2003-12-19 2004-01-21 Amersham Biosciences Uk Ltd Cytochrome C protein and assay
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
AU2005250333A1 (en) * 2004-04-16 2005-12-15 Genentech, Inc. Omi PDZ modulators
AU2005250369A1 (en) * 2004-05-18 2005-12-15 Genentech, Inc. M13 virus major coat protein variants for C-terminal and BI-terminal display of a heterologous protein
US8586294B2 (en) 2004-05-18 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Detection of protein translocation by beta-galactosidase reporter fragment complementation
US20090221440A1 (en) * 2004-07-12 2009-09-03 Board Of Regents, The University Of Texas System Methods and compositions related to identifying protein-protein interactions
EP2336177A1 (de) 2004-08-04 2011-06-22 Amgen, Inc DKK-1 Antikörper
US20060040338A1 (en) 2004-08-18 2006-02-23 Odyssey Thera, Inc. Pharmacological profiling of drugs with cell-based assays
WO2006030442A2 (en) 2004-09-16 2006-03-23 Gamida-Cell Ltd. Methods of ex vivo progenitor and stem cell expansion by co-culture with mesenchymal cells
US20060094059A1 (en) * 2004-09-22 2006-05-04 Odyssey Thera, Inc. Methods for identifying new drug leads and new therapeutic uses for known drugs
EP1797427A4 (de) * 2004-09-22 2009-04-08 Odyssey Thera Inc Verfahren zur identifizierung neuer arzneimittel-leads und neuer therapeutischer verwendungen für bekannte arzneimittel
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060094682A1 (en) 2004-10-29 2006-05-04 Odyssey Thera, Inc. Kinase inhibitors for the treatment of diabetes and obesity
CA2585549A1 (en) 2004-11-18 2006-05-26 Vib Vzw Novel type leptin receptor antagonist
US20060160109A1 (en) * 2004-11-22 2006-07-20 Odyssey Thera, Inc. Harnessing network biology to improve drug discovery
US20070212677A1 (en) * 2004-11-22 2007-09-13 Odyssey Thera, Inc. Identifying off-target effects and hidden phenotypes of drugs in human cells
EP1828408A4 (de) * 2004-12-04 2008-08-27 Univ California Los Alamos Nat Nachweissystem für protein-protein-wechselwirkungen unter verwendung von fluoreszenzproteinmikrodomänen
US20060204999A1 (en) * 2005-03-14 2006-09-14 Stephen Macevicz Detecting molecular complexes
US8227572B2 (en) * 2005-08-19 2012-07-24 Commonwealth Scientific And Industrial Research Organisation Arachnocampa luciferases
JP2009513141A (ja) * 2005-10-27 2009-04-02 トラスティーズ オブ ボストン ユニバーシティ タンパク質相補によるリアルタイムインビボ核酸検出
US20090220942A1 (en) * 2005-10-27 2009-09-03 Natalia Broude Activated split-polypeptides and methods for their production and use
GB0523954D0 (en) * 2005-11-24 2006-01-04 Ucb Celltech Bioassays
US8846393B2 (en) 2005-11-29 2014-09-30 Gamida-Cell Ltd. Methods of improving stem cell homing and engraftment
US7601517B2 (en) 2006-01-10 2009-10-13 Stanford University Split protein self complementing fragments, systems, and methods of use thereof
CA2636855C (en) 2006-01-11 2016-09-27 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
WO2008018905A2 (en) * 2006-01-17 2008-02-14 Cellumen, Inc. Method for predicting biological systems responses
US9359635B2 (en) 2006-04-03 2016-06-07 Promega Corporation Permuted and nonpermuted luciferase biosensors
US7695928B2 (en) 2006-04-10 2010-04-13 Genentech, Inc. Disheveled PDZ modulators
US20070254338A1 (en) * 2006-04-24 2007-11-01 Amgen Inc. Method for making recombinant protein using complementation dependent DHFR mutants
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP3031918B1 (de) 2006-05-11 2018-03-14 Raindance Technologies Inc. Mikrofluidische vorrichtungen
JP5406019B2 (ja) 2006-05-17 2014-02-05 セルーメン、インコーポレイテッド 自動化組織分析のための方法
US8178654B2 (en) 2006-05-23 2012-05-15 Stanford University Estrogen receptor intramolecular folding systems, estrogen receptor intramolecular folding sensors, methods of use thereof, methods of detecting ER ligands, and methods of detecting ER agonists and antagonists
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
MX2009004464A (es) * 2006-10-27 2009-11-02 Univ Boston Conjugados biomoleculares divididos, dirigidos para el tratamiento de enfermedades, malignidades y desordenes, y metodos para su produccion.
EP2095119A2 (de) * 2006-11-10 2009-09-02 Cellumen, Inc. Biosensoren für protein-protein-interaktion und verfahren zu ihrer verwendung
EP2118304A4 (de) * 2007-02-05 2010-04-28 Panbio Ltd Homogene in-vitro-fec-testverfahren und komponenten
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20090075313A1 (en) * 2007-05-04 2009-03-19 Stanford University Split protein fragments, split protein systems, methods of making split protein systems, and methods of using split protein systems
EP2177611B1 (de) 2007-08-01 2013-11-20 Japan Science and Technology Agency Verfahren zur messung der antigenkonzentration
WO2009026303A1 (en) 2007-08-21 2009-02-26 Amgen Inc. Human c-fms antigen binding proteins
US8986997B2 (en) * 2007-08-31 2015-03-24 The Regents Of The University Of Michigan Methods and compositions for increasing biological molecule stability
TW200918553A (en) 2007-09-18 2009-05-01 Amgen Inc Human GM-CSF antigen binding proteins
EP2210104A4 (de) * 2007-11-01 2011-02-23 Univ Arizona State Zellfreie verfahren zum nachweis von protein-ligand-wechselwirkungen
EP2220247A4 (de) 2007-11-16 2011-10-26 Nuvelo Inc Antikörper gegen lrp6
WO2009111073A2 (en) * 2008-03-06 2009-09-11 Odyssey Thera, Inc. High-content and high throughput assays for identification of lipid-regulating pathways, and novel therapeutic agents for lipid disorders
WO2009142735A2 (en) 2008-05-19 2009-11-26 Promega Corporation LUCIFERASE BIOSENSORS FOR cAMP
EP2315629B1 (de) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Tröpfchenbibliotheken
JP5739807B2 (ja) * 2008-08-18 2015-06-24 ディスカヴァーエックス コーポレイション 受容体チロシンキナーゼアッセイ
JP5954990B2 (ja) 2008-11-03 2016-07-20 モレキュラー・パートナーズ・アーゲーＭｏｌｅｃｕｌａｒ Ｐａｒｔｎｅｒｓ Ａｇ Ｖｅｇｆ−ａレセプター相互作用を阻害する結合タンパク質
US9388406B2 (en) 2008-11-17 2016-07-12 Cornell University System useful for reporting protein-protein interactions in the bacterial periplasm
JO3382B1 (ar) 2008-12-23 2019-03-13 Amgen Inc أجسام مضادة ترتبط مع مستقبل cgrp بشري
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US8871686B2 (en) 2009-07-07 2014-10-28 Agency For Science, Technology And Research Methods of identifying a pair of binding partners
US8524457B2 (en) 2009-09-22 2013-09-03 William Patterson Method for the selection of specific affinity binders by homogeneous noncompetitive assay
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
JO3244B1 (ar) 2009-10-26 2018-03-08 Amgen Inc بروتينات ربط مستضادات il – 23 البشرية
UA109888C2 (uk) 2009-12-07 2015-10-26 ІЗОЛЬОВАНЕ АНТИТІЛО АБО ЙОГО ФРАГМЕНТ, ЩО ЗВ'ЯЗУЄТЬСЯ З β-КЛОТО, РЕЦЕПТОРАМИ FGF І ЇХНІМИ КОМПЛЕКСАМИ
EP2517025B1 (de) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Verfahren zur reduzierung des austauschs von molekülen zwischen tröpfchen
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
MX2012011986A (es) 2010-04-15 2013-03-05 Amgen Inc RECEPTOR FGF HUMANO Y PROTEINAS ENLAZADAS A ß-KLOTHO.
AR081361A1 (es) 2010-04-30 2012-08-29 Molecular Partners Ag Proteinas de union modificadas que inhiben la interaccion de receptor del factor de crecimiento endotelial vascular de glicoproteina a vegf-a
WO2011143339A1 (en) 2010-05-11 2011-11-17 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US9290794B2 (en) 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
AR083740A1 (es) 2010-10-27 2013-03-20 Amgen Inc Anticuerpos dkk1 (dickkopf-1) y metodos de uso
CA2818990C (en) 2010-11-26 2021-06-15 Molecular Partners Ag Designed repeat proteins binding to serum albumin
EP2673614B1 (de) 2011-02-11 2018-08-01 Raindance Technologies, Inc. Verfahren zur bildung gemischter tröpfchen
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
WO2012118903A2 (en) 2011-03-01 2012-09-07 Amgen Inc. Bispecific binding agents
EP2686688B1 (de) 2011-03-17 2019-05-08 Cernostics, Inc. Systeme und zusammensetzungen zur diagnose von barrett-ösophagen und verwendungsverfahren dafür
EP2702069A4 (de) 2011-04-29 2015-04-29 Janssen Biotech Inc Il4/il13-bindende repeat-proteine und verwendungen davon
EP2714970B1 (de) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzymquantifizierung
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9574002B2 (en) 2011-06-06 2017-02-21 Amgen Inc. Human antigen binding proteins that bind to a complex comprising β-Klotho and an FGF receptor
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013033080A1 (en) * 2011-08-29 2013-03-07 Wayne State University Device and method for optimizing photobiological processes
MX2014004027A (es) 2011-10-14 2014-04-30 Genentech Inc Activadores de zimogeno.
CA2858572C (en) 2011-12-08 2023-01-17 Amgen Inc. Human lcat antigen binding proteins and their use in therapy
CN104204192A (zh) 2012-02-13 2014-12-10 加米达细胞有限公司 间充质干细胞调理的基质及其产生和使用方法
EP2825549B1 (de) 2012-03-16 2018-10-10 F. Hoffmann-La Roche SA Gleichmässig stabilisierte manipulierte proteine
US9139863B2 (en) 2012-03-16 2015-09-22 Genentech, Inc. Engineered conformationally-stabilized proteins
IL307989A (en) 2012-04-27 2023-12-01 Novo Nordisk As Human CD30 ligand antigen-binding proteins
BR112014032316A2 (pt) 2012-06-28 2017-06-27 Molecular Partners Ag proteínas de repetição de anquirina projetadas que se ligam ao fator de crescimento derivado de plaqueta
US9567569B2 (en) 2012-07-23 2017-02-14 Gamida Cell Ltd. Methods of culturing and expanding mesenchymal stem cells
US9175266B2 (en) 2012-07-23 2015-11-03 Gamida Cell Ltd. Enhancement of natural killer (NK) cell proliferation and activity
EP2738180A1 (de) 2012-11-30 2014-06-04 Molecular Partners AG Bindeproteine mit mindestens zwei Bindedomänen gegen HER2
US20140271629A1 (en) 2013-03-14 2014-09-18 Amgen Inc. Chrdl-1 antigen binding proteins and methods of treatment
SG11201507306VA (en) 2013-03-15 2015-10-29 Promega Corp Activation of bioluminescence by structural complementation
EP3683237A1 (de) 2013-03-15 2020-07-22 Amgen Inc. Humane pac1-antikörper
WO2014144553A1 (en) 2013-03-15 2014-09-18 Amgen Inc. Secreted frizzle-related protein 5 (sfrp5) binding proteins and methods of treatment
TW201605896A (zh) 2013-08-30 2016-02-16 安美基股份有限公司 Gitr抗原結合蛋白
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
EP3094974B1 (de) 2014-01-14 2019-02-27 Asedasciences AG Identifizierung von funktionszellzuständen
AU2015224552A1 (en) * 2014-03-03 2016-09-15 Novogy, Inc. Reducing horizontal gene transfer of functional proteins
WO2015192089A1 (en) 2014-06-12 2015-12-17 Axion Biosystems, Inc. Multiwell microelectrode array with optical stimulation
CN106605382B (zh) * 2014-08-27 2020-11-06 Lg 电子株式会社 在无线通信系统中的发送数据的方法及用于其装置
CN105406948B (zh) * 2014-09-12 2020-03-06 中兴通讯股份有限公司 一种实现并行多用户数据传输的方法及主节点
WO2016040767A2 (en) 2014-09-12 2016-03-17 Amgen Inc. Chrdl-1 epitopes and antibodies
CA2963228C (en) * 2014-10-01 2021-05-04 Lg Electronics Inc. Data transmission method in wireless communication system and device therefor
US9942055B2 (en) * 2015-02-12 2018-04-10 Intel IP Corporation Apparatus, system and method of multicast communication
JOP20200116A1 (ar) 2015-04-24 2017-06-16 Amgen Inc طرق لعلاج أو الوقاية من الصداع النصفي
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
MX2018007859A (es) 2015-12-23 2018-11-09 Amgen Inc Metodo para tratar o mejorar trastornos metabolicos con proteinas de union para el receptor peptidico inhibidor gastrico (gipr) en combinacion con agonistas de glp-1.
WO2017182437A1 (en) 2016-04-18 2017-10-26 Universität Innsbruck Quantification of an interaction between a ras protein and a raf protein
US10239891B2 (en) * 2017-05-15 2019-03-26 Indicator Systems International, Inc. Compositions to detect remnant cancer cells
WO2018026965A1 (en) 2016-08-02 2018-02-08 Isi Life Sciences, Inc. Compositions and methods for detecting cancer cells in a tissue sample
EP3497231B1 (de) * 2016-08-08 2023-10-04 Technology Innovation Momentum Fund (Israel) Limited Partnership Bakterielle systeme zur analyse von ubiquitinierten polypeptiden
WO2018060415A1 (en) 2016-09-29 2018-04-05 Universität Innsbruck Full length kinase activity-conformation reporter
AU2018206279B2 (en) 2017-01-06 2020-09-03 Abl Bio Inc. Anti-alpha-syn antibody and use thereof
JOP20190177A1 (ar) 2017-01-17 2019-07-16 Amgen Inc طريقة لعلاج أو تحسين اضطرابات أيضية باستخدام مساعدات مستقبل glp-1 مقترنة بمناهضات لمستقبل ببتيد مثبط معوي (gipr)
US10753942B2 (en) 2017-05-15 2020-08-25 Indicator Systems International, Inc. Methods to detect remnant cancer cells
JOP20190259A1 (ar) 2017-05-31 2019-10-31 Amgen Inc بروتينات ربط مولد ضد مضادة لـ jagged1
WO2018237097A1 (en) 2017-06-20 2018-12-27 Amgen Inc. METHOD OF TREATING OR REDUCING METABOLIC DISORDERS USING GASTRIC INHIBITING PEPTIDE RECEPTOR BINDING PROTEINS (GIPR) IN ASSOCIATION WITH GLP-1 AGONISTS
CA3071852A1 (en) 2017-08-04 2019-02-07 Amgen Inc. Method of conjugation of cys-mabs
US11932846B2 (en) 2017-08-08 2024-03-19 Technology Innovation Momentum Fund (Israel) Limited Partnership Chloramphenicol resistant split protein and uses thereof
WO2019225787A1 (ko) 2018-05-24 2019-11-28 에이비엘바이오 주식회사 항-b7-h3 항체 및 그 용도
EP4266058A3 (de) 2018-07-06 2024-02-21 The Regents of the University of Colorado, a body corporate Genetisch codiertes system zur konstruktion und zum nachweis biologischer wirkstoffe
CN111198272B (zh) * 2018-11-20 2023-09-08 香港理工大学深圳研究院 体外检测蛋白间相互作用的方法和检测试剂盒及其应用
MX2021006783A (es) 2018-12-10 2021-07-15 Amgen Inc Transposasa de piggybac mutada.
CA3153963A1 (en) * 2019-09-09 2021-03-18 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Methods of restoring lysosomal function of retinal pigment epithelial cells by activation of tfeb
EP4054590A1 (de) 2019-11-04 2022-09-14 Amgen Inc. Verfahren zur behandlung von leukämie
CA3165802A1 (en) * 2020-01-25 2021-07-29 The Trustees Of The University Of Pennsylvania Compositions for small molecule control of precise base editing of target nucleic acids and methods of use thereof
US20230184765A1 (en) 2020-04-17 2023-06-15 Vyriad, Inc. Detection assays for coronavirus neutralizing antibodies
AU2021345124A1 (en) 2020-09-16 2023-03-30 Amgen Inc. Methods for administering therapeutic doses of bispecific T-cell engaging molecules for the treatment of cancer
WO2022060878A1 (en) 2020-09-16 2022-03-24 Amgen Inc. Methods for treating prostate cancer
TW202233660A (zh) 2020-10-30 2022-09-01 美商安進公司 過表現胰島素樣生長因子受體突變體以調節ｉｇｆ補充
CN112375775A (zh) * 2020-11-16 2021-02-19 中南民族大学 一种基于片段化萤光素酶-dna嵌合体互补的发光体系、构建方法及其应用
IL302739A (en) * 2020-11-17 2023-07-01 Nerd Bio Llc High-throughput drug screening methods
WO2022232376A1 (en) 2021-04-29 2022-11-03 Amgen Inc. Methods for reducing low molecular weight species of recombinantly-produced proteins
CA3222782A1 (en) 2021-06-10 2022-12-15 Amgen Inc. Engineered nrg-1 variants with improved selectivity toward erbb4 but not against erbb3
TW202328442A (zh) 2021-09-10 2023-07-16 美商安進公司 平臺宿主對ｉｇｆ—培養基之適應
WO2023183231A1 (en) 2022-03-21 2023-09-28 Amgen Inc. Combination therapy methods with t-cell engaging molecules for treatment of prostate cancer
WO2024064744A2 (en) * 2022-09-20 2024-03-28 Dna Twopointo, Inc. Engineered split dhfr-based methods and systems for selecting cells that have stably acquired a heterologous polynucleotide
WO2024077044A1 (en) 2022-10-05 2024-04-11 Amgen Inc. Combination therapies comprising t-cell redirecting therapies and agonistic anti-il-2r antibodies or fragments thereof
WO2024089297A1 (en) 2022-10-28 2024-05-02 Urban Joerg Novel fluorescent and bioluminescent proteins and reporter systems for ratiometric measurement of luminescence

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5399346A (en) 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
CA2068190C (en) 1991-05-15 1996-12-17 Microgenics Corporation Methods and compositions for enzyme complementation assays using the omega region of beta-galactosidase
US5585237A (en) 1993-10-25 1996-12-17 Creative Biomolecules, Inc. Methods and compositions for high protein production from recombinant DNA
US5585245A (en) * 1994-04-22 1996-12-17 California Institute Of Technology Ubiquitin-based split protein sensor
US5610015A (en) * 1995-03-23 1997-03-11 Wisconsin Alumni Research Foundation System to detect protein-RNA interactions
CA2196496A1 (en) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US6872871B2 (en) * 1998-02-02 2005-03-29 Odyssey Thera Inc. Mapping molecular interactions in plants with protein fragments complementation assays
US6897017B1 (en) * 1997-01-31 2005-05-24 Odyssey Thera Inc. Vivo library-versus-library selection of optimized protein-protein interactions
US6294330B1 (en) * 1997-01-31 2001-09-25 Odyssey Pharmaceuticals Inc. Protein fragment complementation assays for the detection of biological or drug interactions
US6828099B2 (en) * 1998-02-02 2004-12-07 Odyssey Thera Inc. Protein fragment complementation assay (PCA) for the detection of protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. coli TEM-1 β-Lactamase

Also Published As

Publication number Publication date
AU5850598A (en) 1998-08-25
US20070148682A1 (en) 2007-06-28
US20030049688A1 (en) 2003-03-13
US6929916B2 (en) 2005-08-16
DK0966685T3 (da) 2005-12-19
ES2247673T3 (es) 2006-03-01
DE69831136T2 (de) 2006-06-08
EP0966685A1 (de) 1999-12-29
US6270964B1 (en) 2001-08-07
US20040038298A1 (en) 2004-02-26
US7989218B2 (en) 2011-08-02
EP1605042A3 (de) 2011-01-12
CA2196496A1 (en) 1998-07-31
US20110287950A1 (en) 2011-11-24
EP1605042A2 (de) 2005-12-14
EP0966685B1 (de) 2005-08-10
JP2002508832A (ja) 2002-03-19
DE69831136D1 (de) 2005-09-15
US7160691B2 (en) 2007-01-09
WO1998034120A1 (en) 1998-08-06
US20130022999A1 (en) 2013-01-24
US6428951B1 (en) 2002-08-06
US8192944B2 (en) 2012-06-05
JP4262778B2 (ja) 2009-05-13
EP0966685B9 (de) 2006-03-15

Similar Documents

Publication Publication Date Title
ATE301835T1 (de) Eiweisbruchstückergänzungstests zum nachweis biomolekularinteraktionen
Ito et al. Cellular functions, mechanism of action, and regulation of FtsH protease
US5814465A (en) Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method
EP2518155B1 (de) Enzymsensoren, Verfahren zur Herstellung und Anwendung Solcher Sensoren Sowie Verfahren zur Erkennung von Protease-Aktivität
EP1295121B1 (de) Ein biolumineszenz resonanz energie transfer (bret) system mit breitem spektralen resolution zwischen donator- und akzeptor-emission wellenlänge und ihre anwendung
US9347942B2 (en) Ultrasensitive cell based sensors and uses thereof
Subramanian et al. Hemoglobin cleavage site-specificity of the Plasmodium falciparum cysteine proteases falcipain-2 and falcipain-3
WO1995029195A1 (en) Ubiquitin-based split protein sensor
Pfirrmann et al. The prodomain of Ssy5 protease controls receptor-activated proteolysis of transcription factor Stp1
US20060068421A1 (en) Compositions and methods for phage display of polypeptides
WO2000007038A3 (en) Protein fragment complementation assays
Hwang Fu et al. A molecular recognition feature mediates ribosome-induced SRP-receptor assembly during protein targeting
Zhang et al. Sortase A‐mediated synthesis of ligand‐grafted cyclized peptides for modulating a model protein‐protein interaction
CA2427747A1 (en) Affinity maturation by competitive selection
Kudriaeva et al. In-depth characterization of ubiquitin turnover in mammalian cells by fluorescence tracking
WO2003027149A1 (fr) Anticorps et utilisation de ce dernier
WO2003053921A3 (en) Mutant proteinase inhibitors and uses thereof
CN110741083A (zh) 超灵敏的电化学生物传感器
US20050130262A1 (en) Mutated photoproteins and their uses
CA2597545A1 (en) Antibody for assay of adamts13 activity and method for assaying the activity
US20050136488A1 (en) Cellular membrane protein assay
Van der Donk et al. Identification of an active site residue of the R1 subunit of ribonucleotide reductase from Escherichia coli: characterization of substrate-induced polypeptide cleavage by C225SR1
Ha et al. Construction of allosteric protein switches by alternate frame folding and intermolecular fragment exchange
ATE456054T1 (de) Eiweisbruchstückergänzungstests
EP1474526A4 (de) Enzymaktivierungsprotease-assay

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0966685

Country of ref document: EP