ATE216155T1 - Elektroporationsgerät mit benutzerkonfigurierten impulsen - Google Patents

Elektroporationsgerät mit benutzerkonfigurierten impulsen

Info

Publication number
ATE216155T1
ATE216155T1 AT97903934T AT97903934T ATE216155T1 AT E216155 T1 ATE216155 T1 AT E216155T1 AT 97903934 T AT97903934 T AT 97903934T AT 97903934 T AT97903934 T AT 97903934T AT E216155 T1 ATE216155 T1 AT E216155T1
Authority
AT
Austria
Prior art keywords
cells
low voltage
duration
cell
molecules
Prior art date
Application number
AT97903934T
Other languages
English (en)
Inventor
Gunter A Hofmann
Original Assignee
Genetronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetronics Inc filed Critical Genetronics Inc
Application granted granted Critical
Publication of ATE216155T1 publication Critical patent/ATE216155T1/de

Links

Classifications

    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03K—PULSE TECHNIQUE
    • H03K3/00—Circuits for generating electric pulses; Monostable, bistable or multistable circuits
    • H03K3/02—Generators characterised by the type of circuit or by the means used for producing pulses
    • H03K3/53—Generators characterised by the type of circuit or by the means used for producing pulses by the use of an energy-accumulating element discharged through the load by a switching device controlled by an external signal and not incorporating positive feedback
    • H03K3/57—Generators characterised by the type of circuit or by the means used for producing pulses by the use of an energy-accumulating element discharged through the load by a switching device controlled by an external signal and not incorporating positive feedback the switching device being a semiconductor device
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Electromagnetism (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Electrotherapy Devices (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Percussion Or Vibration Massage (AREA)
AT97903934T 1996-09-09 1997-01-27 Elektroporationsgerät mit benutzerkonfigurierten impulsen ATE216155T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/709,615 US5869326A (en) 1996-09-09 1996-09-09 Electroporation employing user-configured pulsing scheme
PCT/US1997/001088 WO1998010515A1 (en) 1996-09-09 1997-01-27 Electroporation employing user-configured pulses

Publications (1)

Publication Number Publication Date
ATE216155T1 true ATE216155T1 (de) 2002-04-15

Family

ID=24850605

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97903934T ATE216155T1 (de) 1996-09-09 1997-01-27 Elektroporationsgerät mit benutzerkonfigurierten impulsen

Country Status (9)

Country Link
US (2) US5869326A (de)
EP (1) EP0925647B1 (de)
JP (1) JP3963955B2 (de)
KR (1) KR100420731B1 (de)
AT (1) ATE216155T1 (de)
CA (1) CA2261703A1 (de)
DE (1) DE69711893T2 (de)
ES (1) ES2175343T3 (de)
WO (1) WO1998010515A1 (de)

Families Citing this family (234)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5636166A (en) * 1995-12-05 1997-06-03 Micron Quantum Devices, Inc. Apparatus for externally timing high voltage cycles of non-volatile memory system
US5914613A (en) * 1996-08-08 1999-06-22 Cascade Microtech, Inc. Membrane probing system with local contact scrub
US6055453A (en) 1997-08-01 2000-04-25 Genetronics, Inc. Apparatus for addressing needle array electrodes for electroporation therapy
US6216034B1 (en) 1997-08-01 2001-04-10 Genetronics, Inc. Method of programming an array of needle electrodes for electroporation therapy of tissue
US6241701B1 (en) 1997-08-01 2001-06-05 Genetronics, Inc. Apparatus for electroporation mediated delivery of drugs and genes
US6135990A (en) * 1997-12-17 2000-10-24 University Of South Florida Electroporation device and method
US6778853B1 (en) 1997-12-17 2004-08-17 University Of South Florida Electroporation device
AU4219399A (en) * 1998-06-08 1999-12-30 Valentis, Inc. Formulations for electroporation
ES2291035T3 (es) * 1998-06-26 2008-02-16 Genetronics, Inc. Sinergismo de efectos dinamicos y de electropermeabilizacion sobre la vitalidad celular como nuevo agente citotoxico.
EP1100579B1 (de) 1998-07-13 2015-09-02 Inovio Pharmaceuticals, Inc. Auf haut und muskel gerrichtete gentherapie durch ein pulsierendes elektrisches feld
US7922709B2 (en) 1998-07-13 2011-04-12 Genetronics, Inc. Enhanced delivery of naked DNA to skin by non-invasive in vivo electroporation
US6678556B1 (en) 1998-07-13 2004-01-13 Genetronics, Inc. Electrical field therapy with reduced histopathological change in muscle
JP2002520101A (ja) 1998-07-13 2002-07-09 ジェネトロニクス、インコーポレーテッド 電気的に補助される化粧用薬剤の局部送達法および装置
US6256882B1 (en) 1998-07-14 2001-07-10 Cascade Microtech, Inc. Membrane probing system
US6192270B1 (en) * 1998-08-14 2001-02-20 Genetronics, Inc. Apparatus and method for the delivery of drugs and genes into tissue
DE69921489T2 (de) 1998-08-31 2005-10-27 Johnson & Johnson Consumer Companies, Inc. Elektrotransportvorrichtung mit klingen
US6150148A (en) * 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
US6937890B2 (en) * 1998-12-17 2005-08-30 University Of South Florida Nonpenetrating electroporation device
US6314316B1 (en) * 1998-12-17 2001-11-06 University Of South Florida Nonpenetrating electroporation device and method
JP4180244B2 (ja) 1999-04-16 2008-11-12 ジョンソン・アンド・ジョンソン・コンシューマー・カンパニーズ・インコーポレイテッド 内部センサを有する電気的な移動による送出装置系
US6578264B1 (en) * 1999-06-04 2003-06-17 Cascade Microtech, Inc. Method for constructing a membrane probe using a depression
AU5764800A (en) 1999-07-01 2001-01-22 Johnson & Johnson Consumer Companies, Inc. Cleansing compositions
US7074747B1 (en) 1999-07-01 2006-07-11 Johnson & Johnson Consumer Companies, Inc. Cleansing compositions
US6762158B2 (en) 1999-07-01 2004-07-13 Johnson & Johnson Consumer Companies, Inc. Personal care compositions comprising liquid ester mixtures
US20050009717A1 (en) * 1999-07-01 2005-01-13 Lukenbach Elvin R. Foaming make-up removing cleansing compositions
EP1254951B1 (de) * 2000-01-27 2011-01-12 The Mollennium Laboratories Molekültransferierende vorrichtung, zusatz für molekültransferierende vorrrichtung und molekül-transferierendes verfahren
US7146210B2 (en) * 2000-02-17 2006-12-05 Standen Ltd. Apparatus and method for optimizing tumor treatment efficiency by electric fields
US7136699B2 (en) * 2002-10-02 2006-11-14 Standen, Ltd. Apparatus for destroying dividing cells
US8447395B2 (en) * 2000-02-17 2013-05-21 Novocure Ltd Treating bacteria with electric fields
US6868289B2 (en) * 2002-10-02 2005-03-15 Standen Ltd. Apparatus for treating a tumor or the like and articles incorporating the apparatus for treatment of the tumor
US7089054B2 (en) * 2002-10-02 2006-08-08 Standen Ltd. Apparatus and method for treating a tumor or the like
US8175698B2 (en) * 2000-02-17 2012-05-08 Novocure Ltd. Treating bacteria with electric fields
US7016725B2 (en) * 2001-11-06 2006-03-21 Standen Ltd. Method and apparatus for destroying dividing cells
US7599746B2 (en) 2000-02-17 2009-10-06 Standen Ltd Apparatus and method for preventing the spread of cancerous metastases and for elimination of metastases
US7890183B2 (en) * 2000-02-17 2011-02-15 Novocure Ltd. Treating parasites with electric fields
EP2335776A1 (de) 2000-02-17 2011-06-22 Yoram Palti Verfahren und vorrichtung zur zerstörung von teilenden zellen
US6714816B1 (en) * 2000-02-22 2004-03-30 University Of South Florida Electroporation and electrophoresis system and method for achieving molecular penetration into cells in vivo
US7742811B2 (en) * 2000-03-13 2010-06-22 Onco Stim Implantable device and method for the electrical treatment of cancer
US8024048B2 (en) * 2000-03-13 2011-09-20 Ionix Medical Inc. Method and device for treating cancer with electrical therapy in conjunction with chemotherapeutic agents and radiation therapy
JP2004532654A (ja) 2000-05-22 2004-10-28 マーク・アンド・カンパニー・インコーポレイテッド 薬剤投与システム性能評価システム及び方法
DE10031179A1 (de) * 2000-06-27 2002-01-31 Amaxa Gmbh Verfahren zur Einbringung von Nukleinsäuren und anderen biologisch aktiven Molekülen in den Kern höherer eukaryontischer Zellen mit Hilfe elektrischen Stroms
US6965226B2 (en) 2000-09-05 2005-11-15 Cascade Microtech, Inc. Chuck for holding a device under test
US6914423B2 (en) 2000-09-05 2005-07-05 Cascade Microtech, Inc. Probe station
DE20114544U1 (de) 2000-12-04 2002-02-21 Cascade Microtech Inc Wafersonde
US6645757B1 (en) 2001-02-08 2003-11-11 Sandia Corporation Apparatus and method for transforming living cells
DE10119901A1 (de) * 2001-04-23 2002-10-24 Amaxa Gmbh Schaltungsanordnung zur Einbringung von Nukleinsäuren und anderen biologisch aktiven Molekülen in den Kern höherer eukaryontischer Zellen mit Hilfe elektrischen Stroms
US6733485B1 (en) 2001-05-25 2004-05-11 Advanced Bionics Corporation Microstimulator-based electrochemotherapy methods and systems
US7127284B2 (en) * 2001-06-11 2006-10-24 Mercator Medsystems, Inc. Electroporation microneedle and methods for its use
US20040204669A1 (en) * 2001-07-05 2004-10-14 Hofmann Gunter A. Apparatus for electroporation mediated delivery for drugs and genes
KR20030012995A (ko) * 2001-08-06 2003-02-14 주식회사 토이랩 Ｄｃ 전원을 사용한 전기천공기
AU2002327490A1 (en) * 2001-08-21 2003-06-30 Cascade Microtech, Inc. Membrane probing system
US20030170898A1 (en) * 2001-12-04 2003-09-11 Gundersen Martin A. Method for intracellular modifications within living cells using pulsed electric fields
US20030129716A1 (en) * 2001-12-06 2003-07-10 Bio-Rad Laboratories, Inc. Automatic electroporation optimization system
AU2003219805B2 (en) * 2002-02-15 2009-06-04 Eisai Inc. Electroporation methods for introducing bioactive agents into cells
US7245963B2 (en) * 2002-03-07 2007-07-17 Advisys, Inc. Electrode assembly for constant-current electroporation and use
WO2003076006A2 (en) * 2002-03-07 2003-09-18 Advisys, Inc. Electrode assembly for constant-current electroporation and use
US8209006B2 (en) * 2002-03-07 2012-06-26 Vgx Pharmaceuticals, Inc. Constant current electroporation device and methods of use
US20030204161A1 (en) * 2002-04-25 2003-10-30 Bozidar Ferek-Petric Implantable electroporation therapy device and method for using same
EP1515775A4 (de) * 2002-05-07 2010-03-03 Oncostim Inc Verfahren und vorrichtung zur behandlung von krebs mit elektrotherapie in verbindung mit chemotherapeutischen mitteln und strahlentherapie
US6929949B1 (en) 2002-06-14 2005-08-16 University Of South Florida Corona ion generating method and apparatus for the manipulation of molecules and biological cells
US7492172B2 (en) 2003-05-23 2009-02-17 Cascade Microtech, Inc. Chuck for holding a device under test
US7057404B2 (en) 2003-05-23 2006-06-06 Sharp Laboratories Of America, Inc. Shielded probe for testing a device under test
US6969604B1 (en) 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
US7250626B2 (en) 2003-10-22 2007-07-31 Cascade Microtech, Inc. Probe testing structure
US8500713B2 (en) * 2003-10-29 2013-08-06 Medtronic, Inc. Implantable electroporation therapy device and method for using same
US7187188B2 (en) 2003-12-24 2007-03-06 Cascade Microtech, Inc. Chuck with integrated wafer support
GB2425844B (en) 2003-12-24 2007-07-11 Cascade Microtech Inc Active wafer probe
US20050222646A1 (en) * 2004-04-06 2005-10-06 Kai Kroll Method and device for treating cancer with modified output electrical therapy
US20050282265A1 (en) * 2004-04-19 2005-12-22 Laura Vozza-Brown Electroporation apparatus and methods
EP1740268A2 (de) * 2004-04-23 2007-01-10 Standen Ltd. Behandlung eines tumors oder ähnlichem mit elektrischen feldern bei unterschiedlicher frequenz
EP1743030A2 (de) * 2004-05-06 2007-01-17 The Trustees of Dartmouth College Methode und apparat zur bakteriellen transformation durch elektroporation mit wellenformen gepulster radiofrequenz beinhaltend ein spektrum von 3 bis 126 mhz
ES2465467T3 (es) * 2004-06-14 2014-06-05 Lonza Cologne Ag Procedimiento y disposición de circuito para el tratamiento de material biológico
TW200613554A (en) 2004-06-17 2006-05-01 Wyeth Corp Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV
JP4980903B2 (ja) * 2004-07-07 2012-07-18 カスケード マイクロテック インコーポレイテッド 膜懸垂プローブを具えるプローブヘッド
WO2006031646A2 (en) 2004-09-13 2006-03-23 Cascade Microtech, Inc. Double sided probing structures
US7776373B2 (en) * 2004-11-19 2010-08-17 Eteka Llc Apparatus and method for the enhancement of food properties and food prepared therefrom
DK1833552T3 (da) * 2004-12-07 2010-08-02 Standen Ltd Elektroder til anbringelse af et elektrisk felt in-vivo i en længere tidsperiode
CN102488967B (zh) * 2004-12-27 2016-03-30 诺沃库勒有限公司 利用电场从不同方向治疗肿瘤等
SG123643A1 (en) * 2004-12-28 2006-07-26 Nanyan Polytechnic Programmable apparatus and method for optimizing and real time monitoring of gene transfection basedon user configured arbitrary waveform pulsing tra in
US7656172B2 (en) 2005-01-31 2010-02-02 Cascade Microtech, Inc. System for testing semiconductors
US7535247B2 (en) 2005-01-31 2009-05-19 Cascade Microtech, Inc. Interface for testing semiconductors
EP1741778B1 (de) * 2005-07-07 2007-12-26 Amaxa AG Verfahren zur Behandlung geringer Volumina mit elektrischem Strom
CN112402798B (zh) * 2005-10-03 2021-11-16 诺沃库勒有限责任公司 优化电场特征以增加电场在增殖细胞上的效果
FR2893256B1 (fr) * 2005-11-14 2008-08-15 Yves Scherman Generateur d'impulsions electriques unipolaires.
CA2632970C (en) * 2006-01-03 2012-01-24 Steven W. Kovalcheck System for dissociation and removal of proteinaceous tissue
US20070232984A1 (en) * 2006-03-30 2007-10-04 Michael Lovell Hand-held electrical stimulation device
US8019414B2 (en) 2006-04-05 2011-09-13 Novocure Ltd. Treating cancer using electromagnetic fields in combination with other treatment regimens
US7403028B2 (en) 2006-06-12 2008-07-22 Cascade Microtech, Inc. Test structure and probe for differential signals
US7764072B2 (en) 2006-06-12 2010-07-27 Cascade Microtech, Inc. Differential signal probing system
US7723999B2 (en) 2006-06-12 2010-05-25 Cascade Microtech, Inc. Calibration structures for differential signal probing
US20080012578A1 (en) * 2006-07-14 2008-01-17 Cascade Microtech, Inc. System for detecting molecular structure and events
PT2066399T (pt) 2006-10-17 2019-01-11 Inovio Pharmaceuticals Inc Dispositivos de eletroporação e métodos de usar os mesmos para eletroporação de células em mamíferos
DK2167194T3 (en) 2007-03-06 2017-06-19 Novocure Ltd TREATMENT OF CANCER USING ELECTROMAGNETIC FIELDS IN COMBINATION WITH PHOTODYNAMIC THERAPY
US7876114B2 (en) 2007-08-08 2011-01-25 Cascade Microtech, Inc. Differential waveguide probe
EP2212425A2 (de) 2007-11-22 2010-08-04 Biontex Laboratories Gmbh Verbesserung von transfektionsergebnissen nicht-viraler genliefersysteme durch beeinflussung des angeborenen immunsystems
DE102007056488A1 (de) 2007-11-22 2009-07-23 Biontex Laboratories Gmbh Steigerung von Transfektionseffizienzen nicht-viraler Genliefersysteme durch Blockierung des angeborenen Immunsystems
DE102008023913A1 (de) 2008-05-16 2009-11-19 Biontex Laboratories Gmbh Verbesserung von Transfektionsergebnissen nicht-viraler Genliefersysteme durch Beeinflussung des angeborenen Immunsystems
DE102008016275A1 (de) 2008-03-28 2009-11-19 Biontex Laboratories Gmbh Verbesserung von Transfektionsergebnissen nicht-viraler Genliefersysteme durch Blockierung des angeborenen Immunsystems
US20110045001A1 (en) * 2008-03-28 2011-02-24 Biontex Laboratories Gmbh Transfection results of non-viral gene delivery systems by influencing of the innate immune system
US9211155B2 (en) * 2008-08-20 2015-12-15 Prostacare Pty Ltd. Non-thermal ablation system for treating BPH and other growths
US8222909B2 (en) 2008-08-22 2012-07-17 Bio-Rad Laboratories, Inc. Electroporation system with dial-in time constant
US7888957B2 (en) * 2008-10-06 2011-02-15 Cascade Microtech, Inc. Probing apparatus with impedance optimized interface
WO2010059247A2 (en) 2008-11-21 2010-05-27 Cascade Microtech, Inc. Replaceable coupon for a probing apparatus
US8319503B2 (en) 2008-11-24 2012-11-27 Cascade Microtech, Inc. Test apparatus for measuring a characteristic of a device under test
US20110118729A1 (en) * 2009-11-13 2011-05-19 Alcon Research, Ltd High-intensity pulsed electric field vitrectomy apparatus with load detection
US20110118734A1 (en) * 2009-11-16 2011-05-19 Alcon Research, Ltd. Capsularhexis device using pulsed electric fields
US20110135626A1 (en) * 2009-12-08 2011-06-09 Alcon Research, Ltd. Localized Chemical Lysis of Ocular Tissue
US20110144562A1 (en) * 2009-12-14 2011-06-16 Alcon Research, Ltd. Localized Pharmacological Treatment of Ocular Tissue Using High-Intensity Pulsed Electrical Fields
US20110144638A1 (en) * 2009-12-14 2011-06-16 Alcon Research, Ltd. Localized Shockwave-Induced Tissue Disruption
WO2011081897A1 (en) * 2009-12-15 2011-07-07 Alcon Research, Ltd. High-intensity pulsed electric field vitrectomy apparatus
JP4713671B1 (ja) * 2010-01-22 2011-06-29 ネッパジーン株式会社 エレクトロポレーション法による外来遺伝子導入法
ES2413557T3 (es) 2010-06-22 2013-07-16 Lonza Cologne Gmbh Procedimiento y disposición de electrodos para tratar células adherentes
US8546979B2 (en) 2010-08-11 2013-10-01 Alcon Research, Ltd. Self-matching pulse generator with adjustable pulse width and pulse frequency
WO2012041867A2 (en) 2010-09-27 2012-04-05 China Agricultural University Combined antigen and dna vaccine for preventing and treating autoimmune diseases
WO2012158132A1 (en) 2011-05-18 2012-11-22 Eteka Llc Apparatus for the enhancement of food properties by electroporation or pulsed electric fields
CN103239734B (zh) 2012-02-10 2016-02-24 北京艾棣维欣生物技术有限公司 用于预防和/或治疗呼吸道合胞病毒感染的疫苗
JP2013198637A (ja) * 2012-03-26 2013-10-03 Toshiyuki Moriizumi エレクロトポレーター用電源
EP3494997B1 (de) 2012-07-25 2019-09-18 The Broad Institute, Inc. Induzierbare dns-bindende proteine und genomperturbationstools sowie anwendungen davon
AU2013344818B2 (en) * 2012-11-14 2017-04-27 International Scientific Pty Ltd A device, system, method, computer program and data signal for the control of a transdermal delivery device
KR20230038607A (ko) 2012-12-13 2023-03-20 더 트러스티스 오브 더 유니버시티 오브 펜실베니아 Ｗｔ１ 백신
US9592303B2 (en) 2013-05-30 2017-03-14 Duke University Enzyme-catalyzed synthesis of site-specific and stoichiometric biomolecule-polymer conjugates
US10392611B2 (en) 2013-05-30 2019-08-27 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
US10364451B2 (en) 2013-05-30 2019-07-30 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
KR20230093536A (ko) 2013-10-07 2023-06-27 더 트러스티스 오브 더 유니버시티 오브 펜실베니아 어쥬번트로서 인터류킨-33을 갖는 백신
US10370664B2 (en) 2013-11-07 2019-08-06 University Of Southern California Use of IKK epsilon inhibitors to activate NFAT and T cell response
KR20220061285A (ko) 2013-11-14 2022-05-12 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 HIV-1 EnV DNA 백신과 단백질 부스터
AU2014354797C1 (en) 2013-11-29 2018-02-01 Inovio Pharmaceuticals, Inc. MERS-CoV vaccine
EP3092004A4 (de) 2014-01-06 2017-02-22 The Trustees Of The University Of Pennsylvania Pd1- und pdl1-antikörper und impfstoffkombinationen und verwendung davon zur immuntherapie
US9708597B2 (en) * 2014-01-17 2017-07-18 General Electric Company Electric pulse generation systems using capacitive coupling
US9592477B2 (en) 2014-05-30 2017-03-14 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by hybrid casting (Ib)
US9593218B2 (en) 2014-05-30 2017-03-14 Pall Corporation Self-assembled structure and membrane comprising block copolymer and process for producing the same by spin coating (IIIa)
US9193835B1 (en) 2014-05-30 2015-11-24 Pall Corporation Self-assembling polymers—IV
US9593217B2 (en) 2014-05-30 2017-03-14 Pall Corporation Self-assembled structure and membrane comprising block copolymer and process for producing the same by spin coating (Va)
US9593219B2 (en) 2014-05-30 2017-03-14 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by spin coating (IIa)
US9604181B2 (en) 2014-05-30 2017-03-28 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by spray coating (IIc)
US9169361B1 (en) 2014-05-30 2015-10-27 Pall Corporation Self-assembling polymers—VI
US9162189B1 (en) 2014-05-30 2015-10-20 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by spin coating (Ia)
US9765171B2 (en) 2014-05-30 2017-09-19 Pall Corporation Self-assembling polymers—V
US9616395B2 (en) 2014-05-30 2017-04-11 Pall Corportaion Membrane comprising self-assembled block copolymer and process for producing the same by spray coating (Ic)
US9598543B2 (en) 2014-05-30 2017-03-21 Pall Corporation Self-assembled structure and membrane comprising block copolymer and process for producing the same by spin coating (VIa)
US9328206B2 (en) 2014-05-30 2016-05-03 Pall Corporation Self-assembling polymers—III
US9163122B1 (en) 2014-05-30 2015-10-20 Pall Corporation Self-assembling polymers—II
US9592476B2 (en) 2014-05-30 2017-03-14 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by hybrid casting (IIb)
US9394407B2 (en) 2014-06-30 2016-07-19 Pall Corporation Hydrophilic block copolymers and membranes prepared therefrom (I)
US9309367B2 (en) 2014-06-30 2016-04-12 Pall Corporation Membranes comprising cellulosic material and hydrophilic block copolymer (V)
US9254466B2 (en) 2014-06-30 2016-02-09 Pall Corporation Crosslinked cellulosic membranes
US9303133B2 (en) 2014-06-30 2016-04-05 Pall Corporation Hydrophilic membranes and method of preparation thereof (IV)
US9718924B2 (en) 2014-06-30 2017-08-01 Pall Corporation Hydrophilic block copolymers and membranes prepared therefrom (II)
US9260569B2 (en) 2014-06-30 2016-02-16 Pall Corporation Hydrophilic block copolymers and method of preparation thereof (III)
US9962662B2 (en) 2014-06-30 2018-05-08 Pall Corporation Fluorinated polymer and use thereof in the preparation of hydrophilic membranes (vi)
KR20230145241A (ko) 2014-10-01 2023-10-17 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 항원 및 어쥬번트로서 인터류킨-21을 갖는 백신
WO2016094874A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Escorted and functionalized guides for crispr-cas systems
US11116838B2 (en) 2015-01-29 2021-09-14 The Trustees Of The University Of Pennsylvania Checkpoint inhibitor and vaccine combinations and use of same for immunotherapy
US11160976B2 (en) 2015-02-17 2021-11-02 Eunsung Global Corp. Skin treatment device using needles
KR101740950B1 (ko) 2015-02-17 2017-06-15 주식회사 은성글로벌 니들을 이용한 피부 치료장치
US10385115B2 (en) 2015-03-26 2019-08-20 Duke University Fibronectin type III domain-based fusion proteins
RU2752834C2 (ru) 2015-06-18 2021-08-09 Те Брод Инститьют, Инк. Мутации фермента crispr, уменьшающие нецелевые эффекты
JP6882782B2 (ja) 2015-08-04 2021-06-02 デューク ユニバーシティ 遺伝子コードされた本質的に無秩序な送達用ステルスポリマーおよびその使用方法
US20190233814A1 (en) 2015-12-18 2019-08-01 The Broad Institute, Inc. Novel crispr enzymes and systems
CN109152818B (zh) 2015-12-21 2023-06-23 杜克大学 具有降低的抗原性的聚合物结合物及其使用方法
US11752213B2 (en) 2015-12-21 2023-09-12 Duke University Surfaces having reduced non-specific binding and antigenicity
WO2017173398A1 (en) 2016-04-01 2017-10-05 Duke University Alpha-helical peptide nanofibers as a self-adjuvanting vaccine platform
KR102670601B1 (ko) 2016-04-19 2024-05-29 더 브로드 인스티튜트, 인코퍼레이티드 신규한 crispr 효소 및 시스템
WO2017189308A1 (en) 2016-04-19 2017-11-02 The Broad Institute Inc. Novel crispr enzymes and systems
US11286478B2 (en) 2016-04-19 2022-03-29 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
US20190284263A1 (en) 2016-04-29 2019-09-19 Inovio Pharmaceuticals, Inc. In vivo use of chondroitinase and/or hyaluronidase to enhance delivery of an agent
US11467156B2 (en) 2016-06-01 2022-10-11 Duke University Nonfouling biosensors
US20210222164A1 (en) 2016-06-29 2021-07-22 The Broad Institute, Inc. Crispr-cas systems having destabilization domain
US10233419B2 (en) 2016-06-30 2019-03-19 Zymergen Inc. Apparatuses and methods for electroporation
CN109890833A (zh) 2016-09-14 2019-06-14 杜克大学 用于递送亲水性药物的基于三嵌段多肽的纳米粒子
CN110023326A (zh) 2016-09-23 2019-07-16 杜克大学 具有lcst行为的非结构化无重复多肽
US10376495B2 (en) 2016-11-23 2019-08-13 University Of South Florida Small molecules that mimic or antagonize actions of granulocyte colony-stimulating-factor (G-CSF)
WO2018119090A1 (en) 2016-12-22 2018-06-28 Ohio State Innovation Foundation Interpentrating microstructures for nanochannel-based cargo delivery
WO2018132732A1 (en) 2017-01-12 2018-07-19 Duke University Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature triggered hierarchical self-assembly
US10813935B2 (en) 2017-02-23 2020-10-27 Transgenex Nanobiotech, Inc. Methods and compositions for treating drug resistance in cancer
US10272052B2 (en) 2017-02-24 2019-04-30 University Of South Florida Compositions and methods for the treatment of tauopathies
US11318155B2 (en) 2017-02-24 2022-05-03 University Of South Florida Hsp90 activator Aha1 drives production of pathological tau aggregates
WO2018183908A1 (en) 2017-03-31 2018-10-04 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating ovarian tumors
US20200071773A1 (en) 2017-04-12 2020-03-05 Massachusetts Eye And Ear Infirmary Tumor signature for metastasis, compositions of matter methods of use thereof
JP6518971B2 (ja) * 2017-05-12 2019-05-29 株式会社ベックス エレクトロポレーター用電源
WO2018213320A1 (en) 2017-05-15 2018-11-22 Duke University Recombinant production of hybrid lipid-biopolymer materials that self-assemble and encapsulate agents
JP7364472B2 (ja) 2017-05-18 2023-10-18 ザ・ブロード・インスティテュート・インコーポレイテッド 標的化された核酸編集のための系、方法、及び組成物
US11897953B2 (en) 2017-06-14 2024-02-13 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
WO2019005884A1 (en) 2017-06-26 2019-01-03 The Broad Institute, Inc. CRISPR / CAS-ADENINE DEAMINASE COMPOSITIONS, SYSTEMS AND METHODS FOR TARGETED NUCLEIC ACID EDITION
WO2019006374A1 (en) 2017-06-30 2019-01-03 Duke University ORDER AND DISORDER AS A DESIGN PRINCIPLE FOR STIMULI-SENSITIVE BIOPOLYMER NETWORKS
GB201710973D0 (en) 2017-07-07 2017-08-23 Avacta Life Sciences Ltd Scaffold proteins
CA3073848A1 (en) 2017-09-21 2019-03-28 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
EP3692152A4 (de) 2017-10-04 2021-12-01 The Broad Institute, Inc. Verfahren und zusammensetzungen zur veränderung der funktion und struktur von chromatinschleifen und/oder -domänen
EP3710039A4 (de) 2017-11-13 2021-08-04 The Broad Institute, Inc. Verfahren und zusammensetzungen zur behandlung von krebs durch targeting des clec2d-klrb1-wegs
SG11202006666XA (en) 2017-11-27 2020-08-28 Prostacare Pty Ltd An apparatus and a method for the treatment of a prostatic disease
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
US11994512B2 (en) 2018-01-04 2024-05-28 Massachusetts Institute Of Technology Single-cell genomic methods to generate ex vivo cell systems that recapitulate in vivo biology with improved fidelity
US11224474B2 (en) 2018-02-28 2022-01-18 Prostacare Pty Ltd System for managing high impedance changes in a non-thermal ablation system for BPH
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
US20210147831A1 (en) 2018-04-27 2021-05-20 The Broad Institute, Inc. Sequencing-based proteomics
WO2019232542A2 (en) 2018-06-01 2019-12-05 Massachusetts Institute Of Technology Methods and compositions for detecting and modulating microenvironment gene signatures from the csf of metastasis patients
WO2020010388A1 (en) * 2018-07-13 2020-01-16 International Scientific Pty Ltd A method and apparatus for user-controlled or practitioner-prescribed transdermal, intradermal or transappendageal delivery of therapeutic, cosmetic or skin care ingredients
EP3829622A4 (de) 2018-08-02 2022-05-11 Duke University Doppel-agonisten-fusionsproteine
EP3833761A1 (de) 2018-08-07 2021-06-16 The Broad Institute, Inc. Neuartige cas12b-enzyme und systeme
US20210324357A1 (en) 2018-08-20 2021-10-21 The Brigham And Women's Hospital, Inc. Degradation domain modifications for spatio-temporal control of rna-guided nucleases
US20210355522A1 (en) 2018-08-20 2021-11-18 The Broad Institute, Inc. Inhibitors of rna-guided nuclease activity and uses thereof
WO2020041380A1 (en) 2018-08-20 2020-02-27 The Broad Institute, Inc. Methods and compositions for optochemical control of crispr-cas9
CA3113095A1 (en) 2018-09-18 2020-03-26 Vnv Newco Inc. Arc-based capsids and uses thereof
WO2020077135A1 (en) 2018-10-10 2020-04-16 Dana-Farber Cancer Institute, Inc. Modulating resistance to bcl-2 inhibitors
US20220411783A1 (en) 2018-10-12 2022-12-29 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
WO2020081730A2 (en) 2018-10-16 2020-04-23 Massachusetts Institute Of Technology Methods and compositions for modulating microenvironment
JP7121419B2 (ja) * 2018-10-29 2022-08-18 株式会社ベックス 気泡噴出方法、気泡噴出用電源装置、および、気泡噴出装置
KR20210104698A (ko) 2018-11-14 2021-08-25 더 브로드 인스티튜트, 인코퍼레이티드 Crispr 시스템 기반 액적 진단 시스템 및 방법
WO2020124050A1 (en) 2018-12-13 2020-06-18 The Broad Institute, Inc. Tiled assays using crispr-cas based detection
CA3124110A1 (en) 2018-12-17 2020-06-25 The Broad Institute, Inc. Crispr-associated transposase systems and methods of use thereof
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
WO2020183984A1 (ja) * 2019-03-14 2020-09-17 株式会社ベックス エレクトロポレーションによるプロトプラストまたは藻類への物質導入方法、および、エレクトロポレーター用電源装置
WO2020191102A1 (en) 2019-03-18 2020-09-24 The Broad Institute, Inc. Type vii crispr proteins and systems
WO2020205681A1 (en) 2019-03-29 2020-10-08 Massachusetts Institute Of Technology Constructs for continuous monitoring of live cells
WO2020206036A1 (en) 2019-04-01 2020-10-08 The Broad Institute, Inc. Novel nucleic acid modifier
US20220175848A1 (en) 2019-04-01 2022-06-09 The Broad Institute, Inc. Methods and compositions for cell therapy
US20220235340A1 (en) 2019-05-20 2022-07-28 The Broad Institute, Inc. Novel crispr-cas systems and uses thereof
US20220220469A1 (en) 2019-05-20 2022-07-14 The Broad Institute, Inc. Non-class i multi-component nucleic acid targeting systems
US20220243178A1 (en) 2019-05-31 2022-08-04 The Broad Institute, Inc. Methods for treating metabolic disorders by targeting adcy5
US11512314B2 (en) 2019-07-12 2022-11-29 Duke University Amphiphilic polynucleotides
WO2021041922A1 (en) 2019-08-30 2021-03-04 The Broad Institute, Inc. Crispr-associated mu transposase systems
EP4230718A3 (de) * 2019-09-02 2023-10-18 Mirai Medical Limited Elektroporationsvorrichtung und -verfahren
US11981922B2 (en) 2019-10-03 2024-05-14 Dana-Farber Cancer Institute, Inc. Methods and compositions for the modulation of cell interactions and signaling in the tumor microenvironment
TW202128775A (zh) 2019-10-16 2021-08-01 英商阿法克塔生命科學有限公司 PD-L1抑制劑-TGFβ抑制劑雙特異性藥物部分
BR112022016507A2 (pt) 2020-02-25 2022-11-22 Inovio Pharmaceuticals Inc Vacinas contra o coronavírus e métodos de uso
US11744885B2 (en) 2020-05-14 2023-09-05 Inovio Pharmaceuticals, Inc. Vaccines for recurrent respiratory papillomatosis and methods of using the same
GB202101299D0 (en) 2020-06-09 2021-03-17 Avacta Life Sciences Ltd Diagnostic polypetides and methods
AU2021289600A1 (en) 2020-06-12 2023-01-19 University Of Rochester Encoding and expression of ACE-tRNAs
WO2022234003A1 (en) 2021-05-07 2022-11-10 Avacta Life Sciences Limited Cd33 binding polypeptides with stefin a protein
WO2023057567A1 (en) 2021-10-07 2023-04-13 Avacta Life Sciences Limited Pd-l1 binding affimers
TW202332694A (zh) 2021-10-07 2023-08-16 英商阿凡克塔生命科學公司 血清半衰期延長之ｐｄ－ｌ１結合多肽
WO2023150753A1 (en) 2022-02-07 2023-08-10 University Of Rochester Optimized sequences for enhanced trna expression or/and nonsense mutation suppression
WO2024102187A1 (en) 2022-11-07 2024-05-16 Pinetree Therapeutics, Inc. Combination therapy comprising bispecific antibodies comprising an nrp1 binding domain
WO2024129459A1 (en) 2022-12-16 2024-06-20 University Of Rochester Repairmen! of barrier dysfunction in esophagus

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3607689A (en) * 1967-10-17 1971-09-21 Inoue K Power supply for large-surface electrochemical machining
US4063146A (en) * 1975-05-22 1977-12-13 Reliance Electric Company Digital firing control for a converter
US4408268A (en) * 1982-08-09 1983-10-04 General Electric Company Pulse modulated electronic voltage controller with smooth voltage output
IT1181297B (it) * 1984-04-19 1987-09-23 Icm Italiana Cost Metalmecc Dispositivo per il controllo automatico dell'alimentazione di un filo tubolare, in particolare in materiale prezioso nell'industria orafa
US4713740A (en) * 1984-07-27 1987-12-15 Sms Advanced Power, Inc. Switch-mode power supply
US4663292A (en) * 1984-12-21 1987-05-05 Wong Daniel T High-voltage biological macromolecule transfer and cell fusion system
US4946793A (en) * 1986-05-09 1990-08-07 Electropore, Inc. Impedance matching for instrumentation which electrically alters vesicle membranes
US4750100A (en) * 1986-06-06 1988-06-07 Bio-Rad Laboratories Transfection high voltage controller
US5098843A (en) * 1987-06-04 1992-03-24 Calvin Noel M Apparatus for the high efficiency transformation of living cells
US5389069A (en) * 1988-01-21 1995-02-14 Massachusetts Institute Of Technology Method and apparatus for in vivo electroporation of remote cells and tissue
US5186800A (en) * 1988-04-18 1993-02-16 Bio-Rad Laboratories, Inc. Electroporation of prokaryotic cells
US4885943A (en) * 1988-05-11 1989-12-12 Hydro-Craft, Inc. Electronic flowmeter system and method
US5047007A (en) * 1989-12-22 1991-09-10 Medtronic, Inc. Method and apparatus for pulsed iontophoretic drug delivery
BE1004328A3 (fr) * 1990-05-16 1992-11-03 Scient Equipment Design & Dev Procede et dispositif de permeabilisation de cellules vivantes.
US5097403A (en) * 1991-02-11 1992-03-17 Astec International Ltd. Current sensing synchronous rectifier apparatus
US5169384A (en) * 1991-08-16 1992-12-08 Bosniak Stephen L Apparatus for facilitating post-traumatic, post-surgical, and/or post-inflammatory healing of tissue
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US5501662A (en) * 1992-05-22 1996-03-26 Genetronics, Inc. Implantable electroporation method and apparatus for drug and gene delivery
US5507724A (en) * 1992-07-01 1996-04-16 Genetronics, Inc. Electroporation and iontophoresis apparatus and method for insertion of drugs and genes into cells
US5304120A (en) * 1992-07-01 1994-04-19 Btx Inc. Electroporation method and apparatus for insertion of drugs and genes into endothelial cells
US5273525A (en) * 1992-08-13 1993-12-28 Btx Inc. Injection and electroporation apparatus for drug and gene delivery
US5315498A (en) * 1992-12-23 1994-05-24 International Business Machines Corporation Apparatus providing leading leg current sensing for control of full bridge power supply
US5439440A (en) * 1993-04-01 1995-08-08 Genetronics, Inc. Electroporation system with voltage control feedback for clinical applications
US5422272A (en) * 1993-07-14 1995-06-06 Andrew A. Papp Improvements to apparatus and method for electroporation
IL108775A (en) * 1994-02-25 2003-09-17 Univ Ramot Method for efficient incorporation of molecules into cells
US5642035A (en) * 1994-06-16 1997-06-24 Bio-Rad Laboratories Transfection high-voltage controller
US5542967A (en) * 1994-10-06 1996-08-06 Ponizovsky; Lazar Z. High voltage electrical apparatus for removing ecologically noxious substances from gases
US5641680A (en) * 1994-11-14 1997-06-24 Zhao; Xi Gene transfer apparatus and method for using the same

Also Published As

Publication number Publication date
DE69711893D1 (de) 2002-05-16
CA2261703A1 (en) 1998-03-12
ES2175343T3 (es) 2002-11-16
JP3963955B2 (ja) 2007-08-22
WO1998010515A1 (en) 1998-03-12
JP2001503208A (ja) 2001-03-06
KR20000035966A (ko) 2000-06-26
EP0925647B1 (de) 2002-04-10
DE69711893T2 (de) 2002-11-14
EP0925647A1 (de) 1999-06-30
US5869326A (en) 1999-02-09
US6096020A (en) 2000-08-01
KR100420731B1 (ko) 2004-03-02

Similar Documents

Publication Publication Date Title
ATE216155T1 (de) Elektroporationsgerät mit benutzerkonfigurierten impulsen
ATE242315T1 (de) Vorrichtung zur elektroporation bei der die temperatur während dieses prozesses kontrolliert wird
US7955827B2 (en) Controlled electroporation and mass transfer across cell membranes
US6117660A (en) Method and apparatus for treating materials with electrical fields having varying orientations
ATE268812T1 (de) Methode und zusammensetzungen zum erlangen reifer dendritischer zellen
DE60044129D1 (de) Kontrolierte elektroporation
CA2196610A1 (en) Flow through electroporation apparatus and method
DE3161494D1 (en) Cosmetic preparations
DE69840739D1 (de) Methode und Zusammensetzung zur Herstellung von reifen dendritischen Zellen
AU551487B2 (en) Culturing human cells in algae extract
WO1998056893A1 (en) Method and apparatus for treating materials with electrical fields having varying orientations
KR830006419A (ko) 고주파 전기 신호에 의한 발효법
Teissié Effects of electric fields and currents on living cells and their potential use in biotechnology: a survey
Adon et al. Development of high voltage pulse inducement method for biological cell
Cope Derivation of the Weber-Fechner law and the Loewenstein equation as the steady-state response of an Elovich solid state biological system
Mischke et al. A versatile low-cost apparatus for cell electrofusion and other electrophysiological treatments
CN219621187U (zh) 一种细胞电穿孔装置
CN217173754U (zh) 一种利用六孔板分割式转染装置
US20030141294A1 (en) Electrofusion chamber
Berg 500—Biological implications of electric field effects: Part V. Fusion of blastomeres and blastocysts of mouse embryos
GB845743A (en) Apparatus for the treatment of micro-organisms
Jamil et al. Optimization of pulse duration parameter for hela cells growth rate
Teissié et al. Electric-field-induced morphological alterations and fusion of hepatocytes
CA2374649A1 (en) Tangential flow, cell concentration and fusion apparatus
Thornton The opposite electrification produced by animal and vegetable life

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties