PT2066399T - Dispositivos de eletroporação e métodos de usar os mesmos para eletroporação de células em mamíferos - Google Patents

Dispositivos de eletroporação e métodos de usar os mesmos para eletroporação de células em mamíferos

Info

Publication number
PT2066399T
PT2066399T PT07839621T PT07839621T PT2066399T PT 2066399 T PT2066399 T PT 2066399T PT 07839621 T PT07839621 T PT 07839621T PT 07839621 T PT07839621 T PT 07839621T PT 2066399 T PT2066399 T PT 2066399T
Authority
PT
Portugal
Prior art keywords
electroporation
mammals
cells
methods
same
Prior art date
Application number
PT07839621T
Other languages
English (en)
Inventor
Draghia-Akli Ruxandra
A Brown Patricia
S Khan Amir
A Pope Melissa
Original Assignee
Inovio Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inovio Pharmaceuticals Inc filed Critical Inovio Pharmaceuticals Inc
Publication of PT2066399T publication Critical patent/PT2066399T/pt

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00Electrotherapy; Circuits therefor
    • A61N1/18Applying electric currents by contact electrodes
    • A61N1/32Applying electric currents by contact electrodes alternating or intermittent currents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00Electrotherapy; Circuits therefor
    • A61N1/18Applying electric currents by contact electrodes
    • A61N1/32Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/327Applying electric currents by contact electrodes alternating or intermittent currents for enhancing the absorption properties of tissue, e.g. by electroporation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00Electrotherapy; Circuits therefor
    • A61N1/02Details
    • A61N1/04Electrodes
    • A61N1/0404Electrodes for external use
    • A61N1/0408Use-related aspects
    • A61N1/0412Specially adapted for transcutaneous electroporation, e.g. including drug reservoirs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00Electrotherapy; Circuits therefor
    • A61N1/02Details
    • A61N1/04Electrodes
    • A61N1/0404Electrodes for external use
    • A61N1/0472Structure-related aspects
    • A61N1/0476Array electrodes (including any electrode arrangement with more than one electrode for at least one of the polarities)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Radiology & Medical Imaging (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Electrotherapy Devices (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
PT07839621T 2006-10-17 2007-10-17 Dispositivos de eletroporação e métodos de usar os mesmos para eletroporação de células em mamíferos PT2066399T (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US85214906P 2006-10-17 2006-10-17
US97898207P 2007-10-10 2007-10-10

Publications (1)

Publication Number Publication Date
PT2066399T true PT2066399T (pt) 2019-01-11

Family

ID=38935920

Family Applications (1)

Application Number Title Priority Date Filing Date
PT07839621T PT2066399T (pt) 2006-10-17 2007-10-17 Dispositivos de eletroporação e métodos de usar os mesmos para eletroporação de células em mamíferos

Country Status (14)

Country Link
US (1) US9452285B2 (pt)
EP (3) EP2409727B1 (pt)
JP (1) JP5439183B2 (pt)
KR (1) KR101421760B1 (pt)
CN (1) CN105457157B (pt)
AU (1) AU2007313203B2 (pt)
CA (1) CA2666501C (pt)
DK (1) DK2066399T3 (pt)
ES (1) ES2703744T3 (pt)
HU (1) HUE042044T2 (pt)
PL (1) PL2409727T3 (pt)
PT (1) PT2066399T (pt)
SG (1) SG175627A1 (pt)
WO (1) WO2008048632A1 (pt)

Families Citing this family (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101444471B1 (ko) * 2006-03-03 2014-09-24 제네트로닉스, 인코포레이티드 외과적 절제술 이후에 조직내 남아 있는 미세 잔류 종양을 치료하는 장치
US7687267B2 (en) * 2006-09-30 2010-03-30 Rational Biotechnology Inc. High-throughput cell transfection device and methods of using thereof
MX2010005229A (es) * 2007-11-12 2010-11-05 Univ Pennsylvania Vacunas novedosas contra sub-tipos multiples de virus de influenza.
WO2009065032A1 (en) * 2007-11-14 2009-05-22 Vgx Pharmaceuticals, Inc. Antibody production elicited by a dna vaccine delivered by electroporation
AU2009231559B2 (en) * 2008-04-04 2015-10-22 The Trustees Of The University Of Pennsylvania Consensus sequences of Chikungunya viral proteins, nucleic acid molecules encoding the same, and compositions and methods for using the same
AU2009231598B2 (en) 2008-04-04 2015-03-12 The Trustees Of The University Of Pennsylvania Vaccines and immunotherapeutics using IL-28 and compositions and methods of using the same
ITCO20080016A1 (it) * 2008-04-22 2009-10-23 Giuseppe Caccia Manipolo per elettroporazione di prodotti farmacologici e/o naturali e/o estetici su strati cutanei a spessore contenuto
KR101609233B1 (ko) 2008-10-29 2016-04-05 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 개량된 hcv 백신 및 이를 이용하는 방법
US8921536B2 (en) 2008-10-29 2014-12-30 The Trustees Of The University Of Pennsylvania HCV vaccines and methods for using the same
CA3001374A1 (en) * 2008-11-17 2010-05-20 Vgx Pharmaceuticals, Llc Antigens that elicit immune response against flavivirus and methods of using same
KR20230114329A (ko) * 2009-04-16 2023-08-01 이노비오 파마수티컬즈, 인크. 비접촉식 전기천공 전극 및 방법
US8903488B2 (en) 2009-05-28 2014-12-02 Angiodynamics, Inc. System and method for synchronizing energy delivery to the cardiac rhythm
US9895189B2 (en) 2009-06-19 2018-02-20 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
WO2011050009A1 (en) * 2009-10-19 2011-04-28 Rational Biotechnology Inc. Method, device and apparatus for inducing self-adjusting cell electroporation
US8298820B2 (en) 2010-01-26 2012-10-30 The Trustees Of The University Of Pennsylvania Influenza nucleic acid molecules and vaccines made therefrom
WO2011109399A1 (en) 2010-03-01 2011-09-09 Inovio Pharmaceuticals, Inc. Multiple tissue layer electroporation applicator and device
WO2012051433A2 (en) 2010-10-13 2012-04-19 Angiodynamics, Inc. System and method for electrically ablating tissue of a patient
CA2825949C (en) 2011-01-28 2019-06-25 The General Hospital Corporation Method and apparatus for discontinuous dermabrasion
EP2667802B1 (en) 2011-01-28 2023-12-20 The General Hospital Corporation Method and apparatus for skin resurfacing
US10278677B2 (en) 2011-01-28 2019-05-07 The General Hospital Corporation Apparatus and method for tissue biopsy
AU2012212264B2 (en) 2011-01-31 2016-01-28 The Trustees Of The University Of Pennsylvania Nucleic acid molecules encoding novel herpes antigens, vaccine comprising the same, and methods of use thereof
ITTO20110374A1 (it) * 2011-04-29 2012-10-30 Igea S P A Metodo di controllo di un dispositivo di elettro-porazione
KR20140068000A (ko) 2011-06-15 2014-06-05 크론테크 파마 아베 주사 바늘 및 장치
WO2014105759A1 (en) * 2012-12-24 2014-07-03 E-Motion Medical, Ltd. Gi tract stimulation devices and methods
US9913978B2 (en) * 2011-06-28 2018-03-13 Kate Broderick Miniminally invasive dermal electroporation device
AU2015203882C1 (en) * 2011-06-28 2017-09-21 Inovio Pharmaceuticals, Inc. A minimally invasive dermal electroporation device
CA2846229C (en) 2011-07-21 2019-08-27 The General Hospital Corporation Method and apparatus for damage and removal of fat
US9078665B2 (en) 2011-09-28 2015-07-14 Angiodynamics, Inc. Multiple treatment zone ablation probe
CN102680526B (zh) * 2012-05-16 2014-07-02 清华大学 单细胞阵列微芯片及其制造、电测量和电穿孔方法
KR101979746B1 (ko) * 2012-09-13 2019-05-17 (주)아모레퍼시픽 전기 천공 장치
US20140171957A1 (en) * 2012-12-19 2014-06-19 Alcon Research, Ltd. Control of Automated Intraocular Lens Injectors
EP4039236A1 (en) 2013-02-20 2022-08-10 Cytrellis Biosystems, Inc. System for tightening a region of skin
KR102349218B1 (ko) 2013-08-09 2022-01-10 사이트렐리스 바이오시스템즈, 인크. 비-열적 조직 절제를 사용한 피부 치료를 위한 방법 및 기구
US10953143B2 (en) 2013-12-19 2021-03-23 Cytrellis Biosystems, Inc. Methods and devices for manipulating subdermal fat
US11260137B2 (en) * 2014-04-06 2022-03-01 Emily Chen Tang Electroporation apparatuses and their method of use
US20160058493A1 (en) * 2014-08-28 2016-03-03 Angiodynamics, Inc. System and method for ablating a tissue site by electroporation with real-time pulse monitoring
EP3217899A4 (en) 2014-11-14 2018-07-11 Cytrellis Biosystems, Inc. Devices and methods for ablation of the skin
EP3277368B1 (en) * 2015-03-31 2020-05-20 OncoSec Medical Incorporated Systems for improved tissue-sensing based electroporation
WO2016161113A1 (en) * 2015-03-31 2016-10-06 University Of South Florida System and method for electroporation controlled by electrical impedance measurements
CN113633852B (zh) 2015-12-28 2023-09-29 因诺维奥制药公司 皮内射流注射型电穿孔装置
ITUB20159819A1 (it) * 2015-12-31 2017-07-01 Biopulse S R L Elettroporatore teranostico ad onda bifasica per la diagnosi delle aree patologiche ed il trasferimento terapeutico di molecole e relativo procedimento
WO2017136758A1 (en) 2016-02-05 2017-08-10 Inovio Pharmaceuticals, Inc. Cancer vaccines and methods of treatment using the same
KR102561605B1 (ko) 2016-03-29 2023-08-01 사이트렐리스 바이오시스템즈, 인크. 미용 피부 리설페이싱용 디바이스 및 방법
EP3443107A1 (en) 2016-04-13 2019-02-20 Synthetic Genomics, Inc. Recombinant arterivirus replicon systems and uses thereof
AU2017330298B2 (en) 2016-09-21 2022-09-29 Cytrellis Biosystems, Inc. Devices and methods for cosmetic skin resurfacing
BR112019007433A2 (pt) 2016-10-17 2019-07-02 Synthetic Genomics Inc sistemas de replicon de vírus recombinante e usos dos mesmos
JP7158820B2 (ja) * 2016-10-26 2022-10-24 ロレアル エレクトロポレーションデバイスおよびエレクトロポレーションデバイスを制御するための方法
US10905492B2 (en) 2016-11-17 2021-02-02 Angiodynamics, Inc. Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode
AU2017372731B2 (en) 2016-12-05 2024-05-23 Janssen Pharmaceuticals, Inc. Compositions and methods for enhancing gene expression
KR20180123312A (ko) * 2017-05-08 2018-11-16 (주)오스힐 미세전류 조절 수단을 구비한 스킨 부스터
JP6518971B2 (ja) * 2017-05-12 2019-05-29 株式会社ベックス エレクトロポレーター用電源
CN107126628A (zh) * 2017-06-14 2017-09-05 重庆百国智科技发展有限公司 一种人体电位差细胞补水及生物电信号修复的装置及其方法
DE102017116305A1 (de) * 2017-07-19 2019-01-24 Cinogy Gmbh Plasma-Behandlungsgerät
SE541651C2 (en) 2017-07-28 2019-11-19 Scandinavian Chemotech Ab An electrode device and a needle electrode for use in delivery of electrical pulses to a desired tissue of a mammal
KR102637862B1 (ko) 2017-12-13 2024-02-19 이노비오 파마수티컬즈, 인크. 메소텔린을 표적으로 하는 암 백신 및 이의 용도
EA202091513A1 (ru) 2017-12-19 2020-09-09 Янссен Сайенсиз Айрлэнд Анлимитед Компани Вакцины против вируса гепатита b (hbv) и их применение
EA202091516A1 (ru) 2017-12-19 2020-11-03 Янссен Сайенсиз Айрлэнд Анлимитед Компани Способы и композиции для индукции иммунного ответа против вируса гепатита b (hbv)
KR20200101416A (ko) 2017-12-19 2020-08-27 얀센 사이언시즈 아일랜드 언리미티드 컴퍼니 B형 간염 바이러스(hbv) 백신 및 그 용도
WO2019123250A1 (en) 2017-12-19 2019-06-27 Janssen Sciences Ireland Unlimited Company Methods and compositions for inducing an immune response against hepatitis b virus (hbv)
US11389531B2 (en) 2017-12-19 2022-07-19 Janssen Sciences Ireland Unlimited Company Methods and apparatus for the delivery of hepatitis B virus (HBV) vaccines
EP3740245A4 (en) 2018-01-19 2022-01-05 Janssen Pharmaceuticals, Inc. INDUCTION AND ENHANCEMENT OF IMMUNE RESPONSES USING RECOMBINATION REPLICON SYSTEMS
KR102132370B1 (ko) * 2018-03-13 2020-08-05 주식회사 지씨에스 피부관리장치, 피부관리장치의 구동방법 및 컴퓨터 판독가능 기록매체
US20210340561A1 (en) * 2018-08-01 2021-11-04 Ohio State Innovation Foundation Compositions and methods for reprogramming skin into insulin producing tissue
EP3673846A1 (en) * 2018-12-27 2020-07-01 Bogdan Ionescu Device for an electrophysiology procedure
CA3141003A1 (en) 2019-06-18 2020-12-24 Helen Horton Combination of hepatitis b virus (hbv) vaccines and pd-l1 inhibitors
WO2020255010A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Combination of recombinant interleukin 12 construct and hepatitis b virus (hbv) vaccines
WO2020255019A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and a quinazoline derivative
US20220324916A1 (en) 2019-06-18 2022-10-13 Janssen Sciences Ireland Unlimited Company Hepatitis B Virus (HBV) Vaccines and Uses Thereof
WO2020255038A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and pyridopyrimidine derivatives
CA3140748A1 (en) 2019-06-18 2020-12-24 Helen Horton Combination of hepatitis b virus (hbv) vaccines and capsid assembly modulators being sulfonamide derivatives
WO2020254876A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Virus-like particle delivery of hepatitis b virus (hbv) vaccines
WO2020255042A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and a pyrimidine derivative
WO2020255013A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and capsid assembly modulators being amide derivatives
WO2020255039A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and quinazoline derivatives
WO2020255035A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and pyrimidine derivatives
AU2020295012A1 (en) 2019-06-18 2022-02-17 Janssen Sciences Ireland Unlimited Company Combination of hepatitis B virus (HBV) vaccines and anti-PD-1 or anti-PD-L1 antibody
WO2020255022A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and aminopyridine derivatives as hpk1 inhibitors
EP3986456A1 (en) 2019-06-18 2022-04-27 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and hbv-targeting rnai
WO2020255021A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and small molecule pdl1 or pd1 inhibitor
WO2020255016A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and dihydropyrimidine derivatives as capsid assembly modulators
US20220249647A1 (en) 2019-06-18 2022-08-11 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and dihydropyrimidine derivatives as capsid assembly modulators
US20220233526A1 (en) 2019-06-18 2022-07-28 Janssen Sciences Ireland Unlimited Company Combination of hepatitis b virus (hbv) vaccines and dihydropyrimidine derivatives as capsid assembly modulators
AU2020296372A1 (en) 2019-06-18 2022-02-17 Janssen Sciences Ireland Unlimited Company Combination of hepatitis B virus (HBV) vaccines and anti-PD-1 antibody
TW202114731A (zh) 2019-06-18 2021-04-16 愛爾蘭商健生科學愛爾蘭無限公司 B型肝炎病毒(HBV)疫苗與靶向HBV之RNAi之組合
MA56520A (fr) 2019-06-18 2022-04-27 Janssen Sciences Ireland Unlimited Co Construction de l'interleukine 12 recombinante et ses utilisations
US20220305118A1 (en) 2019-06-20 2022-09-29 Janssen Sciences Ireland Unlimited Company Carbohydrate nanocarrier delivery of hepatitis b virus (hbv) vaccines
CA3143631A1 (en) 2019-06-20 2020-12-24 Janssen Sciences Ireland Unlimited Company Lipid nanoparticle or liposome delivery of hepatitis b virus (hbv) vaccines
WO2021041979A1 (en) * 2019-08-30 2021-03-04 Virginia Tech Intellectual Properties Inc. Method for intracellular delivery of compounds using cell force and shape with electric fields
EP4121161A1 (en) * 2020-03-20 2023-01-25 Inovio Pharmaceuticals, Inc. Vacuum-assisted electroporation devices, and related systems and methods
CN111581642A (zh) * 2020-05-03 2020-08-25 付贵龙 一种互联网安全防护系统
CA3181680A1 (en) 2020-06-12 2021-12-16 University Of Rochester Encoding and expression of ace-trnas
BR112022027038A2 (pt) 2020-07-08 2023-01-24 Janssen Sciences Ireland Unlimited Co Vacinas de replicon de rna contra hbv
US12006507B2 (en) 2020-07-22 2024-06-11 Nantcell, Inc. Electroporation with active compensation
US20220071692A1 (en) * 2020-09-08 2022-03-10 Biosense Webster (Israel) Ltd. Impedance based irreversible-electroporation (ire)
WO2022133230A1 (en) 2020-12-18 2022-06-23 Janssen Pharmaceuticals, Inc. Combination therapy for treating hepatitis b virus infection
EP4267740A1 (en) 2020-12-28 2023-11-01 Arcturus Therapeutics, Inc. Transcription activator-like effector nucleases (talens) targeting hbv
WO2023150753A1 (en) 2022-02-07 2023-08-10 University Of Rochester Optimized sequences for enhanced trna expression or/and nonsense mutation suppression
US20240017061A1 (en) * 2022-02-25 2024-01-18 Inovio Pharmaceuticals, Inc. Handheld Electroporation Devices, And Related Systems And Methods
WO2023215874A1 (en) * 2022-05-05 2023-11-09 Renbio, Inc. Device related to therapeutic dna delivery
WO2023233290A1 (en) 2022-05-31 2023-12-07 Janssen Sciences Ireland Unlimited Company Rnai agents targeting pd-l1
WO2024129459A1 (en) 2022-12-16 2024-06-20 University Of Rochester Repairmen! of barrier dysfunction in esophagus

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989006555A1 (en) * 1988-01-21 1989-07-27 Massachusetts Institute Of Technology Transport of molecules across tissue using electroporation
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US5702359A (en) 1995-06-06 1997-12-30 Genetronics, Inc. Needle electrodes for mediated delivery of drugs and genes
US5439440A (en) * 1993-04-01 1995-08-08 Genetronics, Inc. Electroporation system with voltage control feedback for clinical applications
US5869326A (en) 1996-09-09 1999-02-09 Genetronics, Inc. Electroporation employing user-configured pulsing scheme
US6055453A (en) 1997-08-01 2000-04-25 Genetronics, Inc. Apparatus for addressing needle array electrodes for electroporation therapy
US6241701B1 (en) * 1997-08-01 2001-06-05 Genetronics, Inc. Apparatus for electroporation mediated delivery of drugs and genes
US6216034B1 (en) * 1997-08-01 2001-04-10 Genetronics, Inc. Method of programming an array of needle electrodes for electroporation therapy of tissue
US6208893B1 (en) * 1998-01-27 2001-03-27 Genetronics, Inc. Electroporation apparatus with connective electrode template
US6120493A (en) * 1998-01-27 2000-09-19 Genetronics, Inc. Method for the introduction of therapeutic agents utilizing an electroporation apparatus
JP2002520101A (ja) * 1998-07-13 2002-07-09 ジェネトロニクス、インコーポレーテッド 電気的に補助される化粧用薬剤の局部送達法および装置
EP2428250A1 (en) * 1998-07-13 2012-03-14 Genetronics, Inc. Skin and muscle-targeted gene therapy by pulsed electrical field
US6972013B1 (en) * 1998-07-13 2005-12-06 Genetronics, Inc. Enhanced delivery of naked DNA to skin by non-invasive in vivo electroporation
US6192270B1 (en) 1998-08-14 2001-02-20 Genetronics, Inc. Apparatus and method for the delivery of drugs and genes into tissue
US6150148A (en) 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
US6148232A (en) * 1998-11-09 2000-11-14 Elecsys Ltd. Transdermal drug delivery and analyte extraction
US7171264B1 (en) * 1999-05-10 2007-01-30 Genetronics, Inc. Intradermal delivery of active agents by needle-free injection and electroporation
US6256533B1 (en) * 1999-06-09 2001-07-03 The Procter & Gamble Company Apparatus and method for using an intracutaneous microneedle array
US6300108B1 (en) * 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
JP2004532654A (ja) * 2000-05-22 2004-10-28 マーク・アンド・カンパニー・インコーポレイテッド 薬剤投与システム性能評価システム及び方法
CN1345610A (zh) * 2000-09-30 2002-04-24 杭州泰士生物科技有限公司 一种活体组织电穿孔用的波形及波形发生器
JP3543326B2 (ja) 2001-08-30 2004-07-14 ソニー株式会社 情報処理装置および方法、情報処理システム、情報配信装置、記録媒体、並びにプログラム
US8209006B2 (en) 2002-03-07 2012-06-26 Vgx Pharmaceuticals, Inc. Constant current electroporation device and methods of use
US7245963B2 (en) 2002-03-07 2007-07-17 Advisys, Inc. Electrode assembly for constant-current electroporation and use
US20080269153A1 (en) * 2002-05-28 2008-10-30 Ruxandra Draghia-Akli Increased stability of a dna formulation by including poly-l-glutamate
US7328064B2 (en) * 2002-07-04 2008-02-05 Inovio As Electroporation device and injection apparatus
US20050070841A1 (en) 2002-07-04 2005-03-31 Inovio As Electroporation device and injection apparatus
EP2364769B1 (en) 2003-05-30 2016-01-13 VGXI, Inc. Devices and methods for biomaterial production

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KR20090107014A (ko) 2009-10-12
EP2066399A1 (en) 2009-06-10
CA2666501C (en) 2017-03-21
US20080091135A1 (en) 2008-04-17
AU2007313203B2 (en) 2012-08-16
ES2703744T3 (es) 2019-03-12
JP5439183B2 (ja) 2014-03-12
EP2066399B1 (en) 2018-10-03
WO2008048632A8 (en) 2008-10-02
EP3524316A1 (en) 2019-08-14
WO2008048632A1 (en) 2008-04-24
PL2409727T3 (pl) 2019-03-29
JP2010506660A (ja) 2010-03-04
CN105457157A (zh) 2016-04-06
KR101421760B1 (ko) 2014-07-22
DK2066399T3 (en) 2019-01-21
AU2007313203A1 (en) 2008-04-24
EP2409727B1 (en) 2018-10-03
CA2666501A1 (en) 2008-04-24
EP2409727A1 (en) 2012-01-25
CN105457157B (zh) 2020-04-14
US9452285B2 (en) 2016-09-27
HUE042044T2 (hu) 2019-06-28
SG175627A1 (en) 2011-11-28

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