JP2001503208A - ユーザが設定したパルスを用いる電気穿孔法 - Google Patents
ユーザが設定したパルスを用いる電気穿孔法Info
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.電気穿孔用パルス波形を生成するための装置であって、前記装置は、 第1の出力電圧を提供する第1の電力供給部と、 第2の出力電圧を提供する第2の電力供給部と、 電磁気的に結合された一次巻線および二次巻線を有する変圧器とを含み、前記 二次巻線は1対の出力端子間に配置され、さらに、 前記第1の電力供給部および前記一次巻線に結合されて、第1のゲーティング 信号に応答して前記第1の電力供給部から前記一次巻線に前記第1の出力電圧を 印加する第1のスイッチと、 前記第2の電力供給部および前記二次巻線に結合されて、第2のゲーティング 信号に応答して前記第2の電力供給部から前記二次巻線に前記第2の出力電圧を 印加する第2のスイッチと、 ユーザ特定の出力パルス波形を受取って前記出力端子に前記特定された出力パ ルス波形を生成するよう前記第1および第2のゲーティング信号を提供するコン トローラとを含む、装置。 2.前記第1の電力供給部は第1の供給信号に比例する大きさを有する第1の出 力電圧を提供し、 前記第2の電力供給部は第2の供給信号に比例する大きさを有する第2の出力 電圧を提供し、 前記コントローラは前記第1および第2の供給信号を提供して前記出力端子に おいて前記特定された出力パルス波形を生成する、請求項1に記載の装置。 3.前記第1および第2の電力供給部の各々は、 スイッチによってエネルギリザーバに選択的に結合される電源を含む、請求項 1に記載の装置。 4.各エネルギリザーバはキャパシタを含む、請求項3に記載の装置。 5.前記スイッチはトランジスタを含む、請求項3に記載の装置。 6.前記スイッチは絶縁ゲートバイポーラ接合トランジスタを含む、請求項3に 記載の装置。 7.前記変圧器は10μヘンリーより低い漏れインダクタンスを有する、請求項 1に記載の装置。 8.前記コントローラはマイクロプロセッサを含む、請求項1に記載の装置。 9.前記コントローラに結合されたユーザインターフェイスをさらに含む、請求 項1に記載の装置。 10.各々が前記出力端子のうち異なる一つに接続される1対の電極をさらに含 む、請求項1に記載の装置。 11.前記出力端子のうち一つと前記二次巻線との間に電気的に挿入されるダイ オードをさらに含む、請求項1に記載の装置。 12.前記第2のスイッチと第2の電力供給部とは直列に結合され、前記直列結 合は前記二次巻線と電気的に並列に取付けられる、請求項1に記載の装置。 13.前記第2のスイッチおよび第2の電力供給部とやはり直列に結合されたダ イオードをさらに含む、請求項12に記載の装置。 14.前記第1のスイッチおよび第1の電力供給部は直列に結合され、前記第2 の結合は前記一次巻線と電気的に並列に取付けられる、請求項1に記載の装置。 15.前記電極は板電極を含む、請求項10に記載の装置。 16.前記電極は針電極を含む、請求項10に記載の装置。 17.前記電極間の距離を感知しかつそれを表わす電子的ギャップ距離出力信号 を提供するギャップセンサをさらに含む、請求項10に記載の装置。 18.電気穿孔用パルスを発生する装置であって、 一次巻線および二次巻線を含む変圧器と、 前記一次巻線に結合されてゲーティング信号の受信に応答して前記一次巻線に わたって予め定められた第1の電圧を配置する第1の電気エネルギ源と、 前記二次巻線に結合され、ゲーティング信号の受信に応答して二次巻線にわた って予め定められた第2の電圧を配置する第2の電気エネルギ源と、 コントローラとを含み、前記コントローラは、 1または複数の出力パルスの出力パルスパターンを特定するユーザ入力を受 取るステップを含み、前記ユーザ入力は各パルスの持続時間を規定しかつ予め定 められた第1の出力レベルまたは予め定められた第2の出力レベルのいずれかを 特定し、さらに、 前記予め定められた第2の出力レベルの各パルスについて、前記特定された 持続時間の間、ゲーティング信号を前記電気エネルギ源のうち一方に印加するス テップと、 前記予め定められた第1の出力レベルの各パルスについて、前記特定の持続 時間の間、前記第2の電気エネルギ源にゲーティング信号を同時に印加しながら 、前記第1の電気エネルギ源にゲーティング信号を印加するステップとを含むス テップを行なうことによって、電気穿孔用パルスパターンを生成するようプログ ラミングされる、装置。 19.電気穿孔用パルスを発生する装置であって、 一次巻線および二次巻線を含む変圧器と、 前記一次巻線に結合されて第1のゲーティング信号に応答して一次巻線にわた って予め定められた第1の電圧を配置する第1の電気エネルギ源と、 前記二次巻線に結合されて第2のゲーティング信号に応答して二次巻線にわた って予め定められた第2の電圧を配置する第2の電気エネルギ源と、 前記第1および第2のエネルギ源に電気的に結合されたコントローラとを含み 、 前記コントローラは、 (a) 前記変圧器の前記二次巻線の端子に第1の電圧を印加することによ って前記二次巻線の端子に第1の電圧を生成するステップと、 (b) ステップ(a)の開始後予め定められた遅延の後に、前記二次巻線 の端子への前記第1の電圧の印加を維持しながら同時に前記一次巻線の端子に第 2の電圧を印加するステップと、 (c) ステップ(b)の開始後予め定められた遅延の後に、前記一次巻線 の端子への前記第2の電圧の印加を終了する一方で予め定められた時間期間、前 記二次巻線の端子への前記第1の電圧の印加を維持するステップとを含む方法ス テップを実行するようプログラミングされる、装置。 20.前記コントローラはさらに、前記ステップ(a)に先立って、 所望の電界を特定する入力を受信するステップと、 電極のギャップ距離を特定する入力を受信するステップと、 前記特定された電極のギャップ距離にわたって前記特定された電界を提供する ための前記第1の電圧を計算するステップとを含む方法ステップを実行するよう プログラミングされる、請求項19に記載の装置。 21.前記変圧器の予め定められた接触子に電気的に結合された1対の電極と、 前記電極間の電極ギャップ距離を感知してそれを表わす電極ギャップ距離出力 信号を提供するギャップヤンサとをさらに含み、 前記コントローラはさらに、 所望の電界の入力を受信するステップと、 前記電極ギャップ距離出力信号を受信するステップと、 前記電極にわたって前記入力された所望の電界を提供する前記第1の電圧を計 算するステップとを実行するようプログラミングされる、請求項19に記載の装 置。 22.一次巻線および二次巻線を含む変圧器を含む電気穿孔用パルス装置を使用 して電気穿孔用パルスパターンを生成するための方法であって、前記方法は、 1または複数の出力パルスの出力パルスパターンを特定するユーザ入力を受信 するステップを含み、前記ユーザ入力は、各パルスの持続時間、および、予め定 められた第1の出力レベルまたは予め定められた第2の出力レベルのいずれかを 特定し、さらに、 前記予め定められた第2の出力レベルの各パルスについて、前記特定された持 続時間の間、前記変圧器の前記二次巻線の端子に予め定められた第1の電圧を印 加するステップと、 前記予め定められた第2のレベルの各パルスについて、前記変圧器の前記二次 巻線の端子に前記予め定められた第1の電圧を印加する一方で同時に前記特定さ れた持続時間の間、前記変圧器の前記一次巻線の端子に予め定められた第2の電 圧を印加するステップとを含む、方法。 23.前記電圧を印加するステップの前に、治療部位のまわりに電極を配置する ステップを含み、前記電極は前記二次巻線の端子にわたって結合され、さらに、 前記電圧を印加するステップの前に、前記治療部位に予め定められた移植薬剤 の分子を供給するステップと、 前記電圧を印加するステップの後に、前記治療部位から前記電極を取除くステ ップとをさらに含む、請求項22に記載の方法。 24.前記治療部位は生体内の組織の1領域を含む、請求項22に記載の方法。 25.前記治療部位は生体から取除かれた細胞を含む、請求項22に記載の方法 。 26.前記ユーザ入力はさらに、前記第1および第2の予め定められた電圧のう ち少なくとも一方を含む、請求項22に記載の方法。 27.変圧器の二次巻線の端子において段階式の電気穿孔用パルスパターンを生 成するための方法であって、前記変圧器はまた、前記二次巻線に電磁気的に結合 されて1対の一次巻線の端子を有する一次巻線を含み、前記方法は、 (a) 前記二次巻線の端子に第1の電圧を印加することによって前記二次巻 線の端子において第1の電圧を生成するステップと、 (b) ステップ(a)の開始後予め定められた遅延の後に、前記二次巻線の 端子への前記第1の電圧の印加を維持しながら同時に前記一次巻線の端子に第2 の電圧を印加するステップと、 (c) ステップ(b)の開始後予め定められた遅延の後に、前記一次巻線の 端子への前記第2の電圧の印加を終了し、かつ、予め定められた時間期間の間、 前記二次巻線の端子への前記第1の電圧の印加を維持するステップとを含む、方 法。 28.前記ステップ(c)の後に、前記変圧器への前記第1および第2の電圧の 印加を終了するステップをさらに含む、請求項27に記載の方法。 29.前記ステップ(a)の前に、生きた細胞の領域のまわりに電極を配置する ステップを含み、前記電極は前記二次巻線の端子に結合され、さらに、 前記ステップ(a)の前に、前記領域に予め定められた移植薬剤の分子を供給 するステップと、 前記ステップ(c)の後に、前記領域から前記電極を取除くステップとをさら に含む、請求項27に記載の方法。 30.前記領域は生体内の組織を含む、請求項29に記載の方法。 31.前記領域は、生体から取除かれた細胞を含む、請求項29に記載の方法。 32.(a) 予め定められた第1の持続時間の間、細胞のある領域に予め定め られた第1の大きさの電界を印加するステップと、 (b) 前記電界を前記予め定められた第1の大きさよりも大きい予め定めら れた第2の大きさに増大するステップと、 (c) 前記電界を前記予め定められた第2の大きさよりも小さい予め定めら れた第3の大きさに減じるステップとを含む、電気穿孔法。 33.(a) 細胞の領域に対して1対の電極を位置付けかつ細胞の前記領域に 予め定められた移植薬剤を供給するステップと、 (b) 予め定められた第1の時間期間の間、前記電極に予め定められた第1 の大きさの電圧を印加することによって、前記移植薬剤の分子を前記細胞の方に 移動させるステップと、 (c) 予め定められた第2の時間期間の間、前記電極に対して前記予め定め られた第1の大きさよりも大きい予め定められた第2の大きさの電圧を印加する ことによって、前記細胞のうち複数の細胞内に孔を開けるステップと、 (d) 前記予め定められた第2の大きさよりも小さい予め定められた第3の 大きさの電圧を印加することによって、前記孔の複数内に前記移植薬剤の分子を 移動させるステップとを含む、電気穿孔法。 34.前記予め定められた第2の大きさの電圧は、前記電極に300〜3000 V/cmの範囲の電界を結果として提供する、請求項33に記載の方法。 35.所望の電界に前記電極間に存在するギャップの測定値を乗じることによっ て、前記電圧の予め定められた第2の大きさを算出するステップをさらに含む、 請求項34に記載の方法。 36.前記電圧の予め定められた第1および第3の大きさは、加熱による前記細 胞への損傷を最小にするよう最小限に抑えられる、請求項33に記載の方法。 37.一次巻線を含む変圧器を含む電気穿孔用パルス装置において電気穿孔用パ ルスパターンを生成するための方法ステップを実行するためのデジタル処理装置 によって実行可能な、機械可読命令のプログラムを具体的に実装する、データ記 憶媒体を含む製造部品であって、前記方法ステップは、 1または複数の出力パルスの出力パルスパターンを特定するユーザ入力を受信 するステップを含み、前記ユーザ入力は、各パルスの持続時間、および、予め定 められた第1の出力レベルまたは予め定められた第2の出力レベルのいずれかを 特定し、さらに、 前記予め定められた第2の出力レベルの各パルスに対して、前記特定された持 続時間の間、前記変圧器の前記二次巻線の端子に予め定められた第1の電圧を印 加するステップと、 前記予め定められた第2のレベルの各パルスに対して、前記変圧器の前記二次 巻線の端子に前記予め定められた第1の電圧を印加する一方で同時に、前記特定 された持続時間の間、前記変圧器の前記一次巻線の端子に予め定められた第2の 電圧を印加するステップとを含む、製造部品。 38.変圧器の二次巻線の端子において段階式の電気穿孔用パルスパターンを生 成するための方法ステップを実行するデジタル処理装置によって実行可能な、機 械可読命令のプログラムを具体的に実装する、データ記憶媒体を含む製造部品で あって、前記変圧器はまた、前記二次巻線に電磁気的に結合されかつ1対の一次 巻線の端子を有する一次巻線を含み、前記方法ステップは、 (a) 前記二次巻線の端子に第1の電圧を印加することによって前記二次巻 線の端子において第1の電圧を生成するステップと、 (b) ステップ(a)の開始後予め定められた遅延の後に、前記二次巻線の 端子への前記第1の電圧の印加を維持しながら、同時に、前記一次巻線の端子に 第2の電圧を印加するステップと、 (c) ステップ(b)の開始後予め定められた遅延の後に、前記一次巻線の 端子への前記第2の電圧の印加を終了し、かつ、予め定められた時間期間の間、 前記二次巻線の端子への前記第1の電圧の印加を維持するステップとを含む、製 造部品。 39.電気穿孔のための方法ステップを実行するデジタル処理装置によって実行 可能な、機械可読命令のプログラムを具体的に実装する、データ記憶媒体を含む 製造部品であって、前記方法ステップは、 (a) 予め定められた第1の時間期間の間、細胞のある領域に予め定められ た第1の大きさの電界を印加するステップと、 (b) 前記電界を前記予め定められた第1の大きさよりも大きい予め定めら れた第2の大きさに増大するステップと、 (c) 前記電界を前記予め定められた第2の大きさよりも小さい予め定めら れた第3の大きさに減じるステップとを含む、製造部品。
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US08/709,615 US5869326A (en) | 1996-09-09 | 1996-09-09 | Electroporation employing user-configured pulsing scheme |
US08/709,615 | 1996-09-09 | ||
PCT/US1997/001088 WO1998010515A1 (en) | 1996-09-09 | 1997-01-27 | Electroporation employing user-configured pulses |
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AT (1) | ATE216155T1 (ja) |
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- 1997-01-27 CA CA002261703A patent/CA2261703A1/en not_active Abandoned
- 1997-01-27 WO PCT/US1997/001088 patent/WO1998010515A1/en active IP Right Grant
- 1997-01-27 KR KR10-1999-7001787A patent/KR100420731B1/ko not_active IP Right Cessation
- 1997-01-27 EP EP97903934A patent/EP0925647B1/en not_active Expired - Lifetime
- 1997-01-27 JP JP51260898A patent/JP3963955B2/ja not_active Expired - Fee Related
- 1997-01-27 ES ES97903934T patent/ES2175343T3/es not_active Expired - Lifetime
- 1997-01-27 DE DE69711893T patent/DE69711893T2/de not_active Expired - Fee Related
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Cited By (8)
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JP2011147399A (ja) * | 2010-01-22 | 2011-08-04 | Nepa Gene Co Ltd | エレクトロポレーション法による外来遺伝子導入法 |
JP2017131772A (ja) * | 2017-05-12 | 2017-08-03 | 俊幸 森泉 | エレクトロポレーター用電源 |
WO2020090312A1 (ja) * | 2018-10-29 | 2020-05-07 | 株式会社ベックス | 気泡噴出方法、気泡噴出用電源装置、および、気泡噴出装置 |
JPWO2020090312A1 (ja) * | 2018-10-29 | 2021-10-14 | 株式会社ベックス | 気泡噴出方法、気泡噴出用電源装置、および、気泡噴出装置 |
JP7121419B2 (ja) | 2018-10-29 | 2022-08-18 | 株式会社ベックス | 気泡噴出方法、気泡噴出用電源装置、および、気泡噴出装置 |
JPWO2020183984A1 (ja) * | 2019-03-14 | 2020-09-17 | ||
WO2020183984A1 (ja) * | 2019-03-14 | 2020-09-17 | 株式会社ベックス | エレクトロポレーションによるプロトプラストまたは藻類への物質導入方法、および、エレクトロポレーター用電源装置 |
JP7377561B2 (ja) | 2019-03-14 | 2023-11-10 | 株式会社ベックス | エレクトロポレーションによるプロトプラストまたは藻類への物質導入方法、および、エレクトロポレーター用電源装置 |
Also Published As
Publication number | Publication date |
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US5869326A (en) | 1999-02-09 |
EP0925647A1 (en) | 1999-06-30 |
DE69711893T2 (de) | 2002-11-14 |
DE69711893D1 (de) | 2002-05-16 |
KR100420731B1 (ko) | 2004-03-02 |
KR20000035966A (ko) | 2000-06-26 |
ES2175343T3 (es) | 2002-11-16 |
WO1998010515A1 (en) | 1998-03-12 |
EP0925647B1 (en) | 2002-04-10 |
US6096020A (en) | 2000-08-01 |
JP3963955B2 (ja) | 2007-08-22 |
CA2261703A1 (en) | 1998-03-12 |
ATE216155T1 (de) | 2002-04-15 |
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