AR070277A1 - Expresion y acumulacion de alto nivel de celobiohidrolasa en tejido vegetal - Google Patents

Expresion y acumulacion de alto nivel de celobiohidrolasa en tejido vegetal

Info

Publication number
AR070277A1
AR070277A1 ARP090100262A ARP090100262A AR070277A1 AR 070277 A1 AR070277 A1 AR 070277A1 AR P090100262 A ARP090100262 A AR P090100262A AR P090100262 A ARP090100262 A AR P090100262A AR 070277 A1 AR070277 A1 AR 070277A1
Authority
AR
Argentina
Prior art keywords
vacuoles
expression
plants
enzyme
accumulation
Prior art date
Application number
ARP090100262A
Other languages
English (en)
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of AR070277A1 publication Critical patent/AR070277A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)

Abstract

Se proporcionan polinucleotidos, composiciones y métodos para aumento de la expresion y/o acumulacion de una enzima celobiohidrolasa en las vacuolas de las células vegetales. El método implica direccionamiento de la enzima a las vacuolas, por el uso de un péptido senal de distribucion a las vacuolas de poliamina-oxidasa de cebada (BpAO). Las plantas transformadas con un constructo de expresion que codifica el péptido senal de distribucion a las vacuolas enlazado operativamente a la enzima celobiohidrolasa dirigen la expresion del polipéptido a las vacuolas de las células vegetales. Se proporcionan plantas, semillas, y tejidos de plantas transgénicas, así como partes de plantas. Los usos de las plantas transgénicas o material vegetal que expresa los constructos incluyen usos agronomicos e industriales, por ejemplo, alimentacion humana, piensos para animales, biocombustible, alcohol industrial, alimentaciones para fermentacion, y análogos.
ARP090100262A 2008-01-30 2009-01-28 Expresion y acumulacion de alto nivel de celobiohidrolasa en tejido vegetal AR070277A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2484408P 2008-01-30 2008-01-30
US4769208P 2008-04-24 2008-04-24

Publications (1)

Publication Number Publication Date
AR070277A1 true AR070277A1 (es) 2010-03-25

Family

ID=40386114

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP090100262A AR070277A1 (es) 2008-01-30 2009-01-28 Expresion y acumulacion de alto nivel de celobiohidrolasa en tejido vegetal
ARP090100280A AR070314A1 (es) 2008-01-30 2009-01-29 Uso de peptidos de transito a plastidos derivados de glaucocystophytas

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP090100280A AR070314A1 (es) 2008-01-30 2009-01-29 Uso de peptidos de transito a plastidos derivados de glaucocystophytas

Country Status (7)

Country Link
US (2) US20090205075A1 (es)
CN (2) CN101960011A (es)
AR (2) AR070277A1 (es)
AU (1) AU2009210590A1 (es)
BR (2) BRPI0907621A2 (es)
CL (2) CL2009000210A1 (es)
WO (2) WO2009099749A1 (es)

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US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
US10443068B2 (en) 2010-06-25 2019-10-15 Agrivida, Inc. Plants with engineered endogenous genes
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Also Published As

Publication number Publication date
CL2009000211A1 (es) 2009-06-05
US20090193541A1 (en) 2009-07-30
WO2009099749A1 (en) 2009-08-13
BRPI0906430A2 (pt) 2015-08-18
AR070314A1 (es) 2010-03-31
WO2009099783A2 (en) 2009-08-13
CL2009000210A1 (es) 2009-06-05
US20090205075A1 (en) 2009-08-13
CN101960011A (zh) 2011-01-26
CN101960013A (zh) 2011-01-26
BRPI0907621A2 (pt) 2019-01-15
AU2009210590A1 (en) 2009-08-13
US8168863B2 (en) 2012-05-01
WO2009099783A3 (en) 2009-10-01

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