AR070314A1 - Uso de peptidos de transito a plastidos derivados de glaucocystophytas - Google Patents

Uso de peptidos de transito a plastidos derivados de glaucocystophytas

Info

Publication number
AR070314A1
AR070314A1 ARP090100280A ARP090100280A AR070314A1 AR 070314 A1 AR070314 A1 AR 070314A1 AR P090100280 A ARP090100280 A AR P090100280A AR P090100280 A ARP090100280 A AR P090100280A AR 070314 A1 AR070314 A1 AR 070314A1
Authority
AR
Argentina
Prior art keywords
plastics
nucleotide sequence
transit peptides
polypeptide
transit
Prior art date
Application number
ARP090100280A
Other languages
English (en)
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of AR070314A1 publication Critical patent/AR070314A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)

Abstract

Se proporcionan composiciones y métodos para direccionamiento de polipéptidos a plásticos. Las composiciones comprenden péptidos de tránsito a plásticos así como secuencias de nucleotidos que codifican tales péptidos de tránsito y sus variantes. Adicionalmente estos constructor de DNA encuentran aplicacion en la expresion y el direccionamiento del polipéptido de interés a un plástido. También casetes de expresion, vectores, plantas transformadas, células de plantas transformadas, y semillas de plantas transformadas establemente en donde un polipéptido de interés está diseccionado a un plástido por dicho péptido de tránsito. Reivindicacion 1: Un ácido nucleico aislado que comprende una primera secuencia de nucleotidos que codifica un péptido de tránsito a las plásticos de una Glaucocistofita en donde dicha primera secuencia de nucleotidos está enlazada operativamente a una segunda secuencia de nucleotidos que codifica un polipéptido heterologo. Reivindicacion 3: El ácido nucleico aislado de la reivindicacion 1 en donde la Glaucosistofita es Cyanaphora paradoxa.
ARP090100280A 2008-01-30 2009-01-29 Uso de peptidos de transito a plastidos derivados de glaucocystophytas AR070314A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2484408P 2008-01-30 2008-01-30
US4769208P 2008-04-24 2008-04-24

Publications (1)

Publication Number Publication Date
AR070314A1 true AR070314A1 (es) 2010-03-31

Family

ID=40386114

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP090100262A AR070277A1 (es) 2008-01-30 2009-01-28 Expresion y acumulacion de alto nivel de celobiohidrolasa en tejido vegetal
ARP090100280A AR070314A1 (es) 2008-01-30 2009-01-29 Uso de peptidos de transito a plastidos derivados de glaucocystophytas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP090100262A AR070277A1 (es) 2008-01-30 2009-01-28 Expresion y acumulacion de alto nivel de celobiohidrolasa en tejido vegetal

Country Status (7)

Country Link
US (2) US20090205075A1 (es)
CN (2) CN101960011A (es)
AR (2) AR070277A1 (es)
AU (1) AU2009210590A1 (es)
BR (2) BRPI0907621A2 (es)
CL (2) CL2009000210A1 (es)
WO (2) WO2009099749A1 (es)

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Also Published As

Publication number Publication date
US20090193541A1 (en) 2009-07-30
US20090205075A1 (en) 2009-08-13
WO2009099749A1 (en) 2009-08-13
WO2009099783A3 (en) 2009-10-01
BRPI0906430A2 (pt) 2015-08-18
US8168863B2 (en) 2012-05-01
CN101960013A (zh) 2011-01-26
AR070277A1 (es) 2010-03-25
CN101960011A (zh) 2011-01-26
WO2009099783A2 (en) 2009-08-13
AU2009210590A1 (en) 2009-08-13
CL2009000211A1 (es) 2009-06-05
BRPI0907621A2 (pt) 2019-01-15
CL2009000210A1 (es) 2009-06-05

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