CL2009000211A1 - Molécula de ácido nucleico que comprende una secuencia de direccionamiento a vacuola de almacenamiento de un polipéptido heterologo; y procedimiento para la acumulacion de dichas proteinas en una planta. - Google Patents

Molécula de ácido nucleico que comprende una secuencia de direccionamiento a vacuola de almacenamiento de un polipéptido heterologo; y procedimiento para la acumulacion de dichas proteinas en una planta.

Info

Publication number
CL2009000211A1
CL2009000211A1 CL2009000211A CL2009000211A CL2009000211A1 CL 2009000211 A1 CL2009000211 A1 CL 2009000211A1 CL 2009000211 A CL2009000211 A CL 2009000211A CL 2009000211 A CL2009000211 A CL 2009000211A CL 2009000211 A1 CL2009000211 A1 CL 2009000211A1
Authority
CL
Chile
Prior art keywords
accumulation
plant
nucleic acid
acid molecule
proteins
Prior art date
Application number
CL2009000211A
Other languages
English (en)
Inventor
Stacy Miles
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of CL2009000211A1 publication Critical patent/CL2009000211A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)

Abstract

Molécula de ácido nucleico que comprende una secuencia de direccionamiento a vacuola de almacenamiento de un polipéptido heterólogo; método para la acumulación de este en una planta.
CL2009000211A 2008-01-30 2009-01-30 Molécula de ácido nucleico que comprende una secuencia de direccionamiento a vacuola de almacenamiento de un polipéptido heterologo; y procedimiento para la acumulacion de dichas proteinas en una planta. CL2009000211A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2484408P 2008-01-30 2008-01-30
US4769208P 2008-04-24 2008-04-24

Publications (1)

Publication Number Publication Date
CL2009000211A1 true CL2009000211A1 (es) 2009-06-05

Family

ID=40386114

Family Applications (2)

Application Number Title Priority Date Filing Date
CL2009000210A CL2009000210A1 (es) 2008-01-30 2009-01-30 Acidos nucleicos que comprenden una secuencia que codifica un peptido de transito a plastidios de una glaucosafita enlazada a una segunda secuencia que codifica un peptido heterologo; metodo para la expresion estable de un polipeptido heterologo en una planta; polipeptido aislado codificado por el acido nucleico descrito anteriormente.
CL2009000211A CL2009000211A1 (es) 2008-01-30 2009-01-30 Molécula de ácido nucleico que comprende una secuencia de direccionamiento a vacuola de almacenamiento de un polipéptido heterologo; y procedimiento para la acumulacion de dichas proteinas en una planta.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CL2009000210A CL2009000210A1 (es) 2008-01-30 2009-01-30 Acidos nucleicos que comprenden una secuencia que codifica un peptido de transito a plastidios de una glaucosafita enlazada a una segunda secuencia que codifica un peptido heterologo; metodo para la expresion estable de un polipeptido heterologo en una planta; polipeptido aislado codificado por el acido nucleico descrito anteriormente.

Country Status (7)

Country Link
US (2) US20090205075A1 (es)
CN (2) CN101960011A (es)
AR (2) AR070277A1 (es)
AU (1) AU2009210590A1 (es)
BR (2) BRPI0907621A2 (es)
CL (2) CL2009000210A1 (es)
WO (2) WO2009099749A1 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008210495B2 (en) * 2007-01-30 2014-02-27 Bp Corporation North America, Inc. Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
EP2154252B1 (en) * 2008-07-16 2014-12-24 Sumitomo Chemical Company, Limited Methods for producing secreted proteins
US20130071884A1 (en) 2009-11-06 2013-03-21 Agrivida, Inc. Plants expressing cell wall degrading enzymes and expression vectors
US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
US10443068B2 (en) 2010-06-25 2019-10-15 Agrivida, Inc. Plants with engineered endogenous genes
EP2601300A1 (en) 2010-08-06 2013-06-12 Danisco US Inc. Production of isoprene under neutral ph conditions
AR082578A1 (es) 2010-08-06 2012-12-19 Danisco Us Inc SACARIFICACION Y FERMENTACION A pH NEUTRO
BR112013018695B1 (pt) 2011-01-26 2021-03-30 Novozymes A / S Célula hospedeira microbiana recombinante, processos para produzir um polipeptídeo, para produzir uma proteína, para degradar um material celulósico, e, construto de ácido nucleico ou vetor de expressão
CN106244622B (zh) 2011-03-07 2020-06-12 谷万达公司 在工程植物中表达多种蛋白的表达盒和表达载体及制备工程植物和生产可溶性糖的方法
CA2834393A1 (en) 2011-04-29 2012-11-01 Danisco Us Inc. Single ph process for starch liquefaction and saccharification for high-density glucose syrups
BR112014002745A8 (pt) 2011-08-05 2017-06-20 Danisco Us Inc produção de isoprenoides sob condições de ph neutro
CN102304544B (zh) * 2011-09-01 2013-03-20 华中农业大学 一种根癌农杆菌介导的大麦茎尖转化方法
CN103998605B (zh) * 2011-12-20 2021-08-03 诺维信股份有限公司 纤维二糖水解酶变体和编码它们的多核苷酸
AR092112A1 (es) 2012-08-16 2015-03-25 Danisco Us Inc METODO DE USAR a-AMILASA DE ASPERGILLUS CLAVATUS Y PULULANASA PARA LA SACARIFICACION
MX2015001823A (es) * 2012-08-16 2015-05-07 Danisco Inc Metodo para usar alfa-amilasa a partir de aspergillus clavatus e isoamilasa para sacarificacion.
CN104754954B (zh) * 2012-08-23 2018-08-28 特郎萨格以色列有限公司 转基因微藻和其用于口服递送蛋白质的用途
WO2014093125A1 (en) 2012-12-14 2014-06-19 Danisco Us Inc. Method of using alpha-amylase from aspergillus fumigatus and isoamylase for saccharification
WO2014099415A1 (en) 2012-12-20 2014-06-26 Danisco Us Inc. Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification
US20140342913A1 (en) * 2013-05-16 2014-11-20 Syngenta Participations Ag Genes providing tolerance to pds inhibitors
WO2015094809A1 (en) 2013-12-19 2015-06-25 Danisco Us Inc. Chimeric fungal alpha-amylases comprising carbohydrate binding module and the use thereof
WO2015121863A1 (en) 2014-02-12 2015-08-20 Transalgae Israel Ltd. Algal based edible vaccines
CN105669847B (zh) * 2015-07-22 2019-05-07 中国农业科学院作物科学研究所 大豆蛋白GmVPS9a1在调控植物贮藏蛋白分选中的应用
WO2017044372A1 (en) * 2015-09-11 2017-03-16 AgBiome, Inc. Chloroplast transit peptides and methods of their use

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5530188A (en) * 1990-03-02 1996-06-25 Amoco Corporation Beta-carotene biosynthesis in genetically engineered hosts
US5168064A (en) * 1990-04-20 1992-12-01 The Regents Of The University Of California Endo-1,4-β-glucanase gene and its use in plants
GB9009307D0 (en) 1990-04-25 1990-06-20 Ici Plc Dna,constructs,cells and plant derived therefrom
DK162790D0 (da) 1990-07-06 1990-07-06 Novo Nordisk As Plantecelle
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content
PL331767A1 (en) * 1996-09-12 1999-08-02 Novartis Ag Transgenous plants expressing cellutitic enzymes
US5981835A (en) * 1996-10-17 1999-11-09 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
US6818803B1 (en) * 1997-06-26 2004-11-16 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
US6972350B1 (en) * 1998-07-15 2005-12-06 The Horticulture And Food Research Institute Of New Zealand Pest-resistant plants comprising a construct encoding a vacuole targeting sequence and avidin or streptavidin
US6013860A (en) * 1998-07-24 2000-01-11 Calgene Llc Expression of enzymes involved in cellulose modification
US7049485B2 (en) * 2000-10-20 2006-05-23 Board Of Trustees Of Michigan State University Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
US20070192900A1 (en) * 2006-02-14 2007-08-16 Board Of Trustees Of Michigan State University Production of beta-glucosidase, hemicellulase and ligninase in E1 and FLC-cellulase-transgenic plants
US8093456B2 (en) * 2000-10-20 2012-01-10 Board Of Trustees Of Michigan State University Transgenic cover plants containing hemicellulase and cellulase which degrade lignin and cellulose to fermentable sugars
CN1821412A (zh) * 2001-08-27 2006-08-23 辛根塔参与股份公司 自加工的植物及植物部分
EP1463815A4 (en) * 2001-09-24 2005-04-20 Prodigene Inc METHOD FOR INCREASING THE EXPRESSION OF HETEROLOGOUS PROTEINS IN PLANTS
US8558058B2 (en) * 2001-12-06 2013-10-15 Applied Biotechnology Institute Monocotyledonous seed expressing exo-1,4B-glucanase
WO2003049538A2 (en) * 2001-12-06 2003-06-19 Prodigene, Inc. Methods for the cost-effective saccharification of lignocellulosic biomass
AU2004318207B2 (en) * 2004-03-08 2009-12-17 Syngenta Participations Ag Self-processing plants and plant parts
KR100769584B1 (ko) * 2004-07-30 2007-10-23 학교법인 포항공과대학교 셀룰로오스의 자가가수분해를 위한 셀룰라아제 발현형질전환 식물체 및 이를 이용한 수용성 당의 생산방법
GB0426512D0 (en) * 2004-12-03 2005-01-05 Gemstar Cambridge Ltd Manipulation of starch granule size and number (II)
EP1861506B1 (en) 2005-03-15 2015-04-15 BP Corporation North America Inc. Cellulases, nucleic acids encoding them and methods for making and using them
WO2007100897A2 (en) * 2006-02-27 2007-09-07 Edenspace System Corporation Energy crops for improved biofuel feedstocks
JP5296684B2 (ja) * 2006-08-11 2013-09-25 モンサント テクノロジー エルエルシー アントラニル酸合成酵素の葉緑体標的発現による高トリプトファントウモロコシの生産
US7838729B2 (en) * 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof

Also Published As

Publication number Publication date
US20090193541A1 (en) 2009-07-30
WO2009099749A1 (en) 2009-08-13
BRPI0906430A2 (pt) 2015-08-18
AR070277A1 (es) 2010-03-25
AR070314A1 (es) 2010-03-31
WO2009099783A2 (en) 2009-08-13
CL2009000210A1 (es) 2009-06-05
US20090205075A1 (en) 2009-08-13
CN101960011A (zh) 2011-01-26
CN101960013A (zh) 2011-01-26
BRPI0907621A2 (pt) 2019-01-15
AU2009210590A1 (en) 2009-08-13
US8168863B2 (en) 2012-05-01
WO2009099783A3 (en) 2009-10-01

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