CL2011003104A1 - Metodo para la elaboracion de acidos grasos no saturados, basado en el cultivo de una celula que expresa un polipeptido heterologo con actividad esaturasa/enoil-coa reductasa celula huesped, complejo elongasa que comprende dicho polipeptido y uso de la misma. - Google Patents

Metodo para la elaboracion de acidos grasos no saturados, basado en el cultivo de una celula que expresa un polipeptido heterologo con actividad esaturasa/enoil-coa reductasa celula huesped, complejo elongasa que comprende dicho polipeptido y uso de la misma.

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Publication number
CL2011003104A1
CL2011003104A1 CL2011003104A CL2011003104A CL2011003104A1 CL 2011003104 A1 CL2011003104 A1 CL 2011003104A1 CL 2011003104 A CL2011003104 A CL 2011003104A CL 2011003104 A CL2011003104 A CL 2011003104A CL 2011003104 A1 CL2011003104 A1 CL 2011003104A1
Authority
CL
Chile
Prior art keywords
polypeptide
cell
enoyl
fatty acids
coa reductase
Prior art date
Application number
CL2011003104A
Other languages
English (en)
Inventor
Olga Sayanova
Jonathen A Napier
Frederic Beaudoin
Original Assignee
Rothamsted Res Ltda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42983387&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CL2011003104(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Rothamsted Res Ltda filed Critical Rothamsted Res Ltda
Publication of CL2011003104A1 publication Critical patent/CL2011003104A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Polinucleótido que comprende una secuencia de ácido nucleico que codifica un polipéptido con actividad deshidratasa/enoil-coa reductasa (necr); vector; célula huésped; método de elaboración; polipéptido; método para elaborar ácidos grasos no saturados y una composición de aceite, lípidos o ácidos grasos.
CL2011003104A 2009-06-08 2011-12-07 Metodo para la elaboracion de acidos grasos no saturados, basado en el cultivo de una celula que expresa un polipeptido heterologo con actividad esaturasa/enoil-coa reductasa celula huesped, complejo elongasa que comprende dicho polipeptido y uso de la misma. CL2011003104A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP09162204 2009-06-08

Publications (1)

Publication Number Publication Date
CL2011003104A1 true CL2011003104A1 (es) 2012-06-01

Family

ID=42983387

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2011003104A CL2011003104A1 (es) 2009-06-08 2011-12-07 Metodo para la elaboracion de acidos grasos no saturados, basado en el cultivo de una celula que expresa un polipeptido heterologo con actividad esaturasa/enoil-coa reductasa celula huesped, complejo elongasa que comprende dicho polipeptido y uso de la misma.

Country Status (10)

Country Link
US (1) US8993841B2 (es)
EP (1) EP2440662B1 (es)
JP (1) JP2012529277A (es)
CN (1) CN102597242B (es)
AU (1) AU2010257729B2 (es)
BR (1) BRPI1010614A2 (es)
CA (1) CA2763107A1 (es)
CL (1) CL2011003104A1 (es)
DE (1) DE112010002353T5 (es)
WO (1) WO2010142522A2 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
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US11952581B2 (en) 2003-08-01 2024-04-09 Basf Plant Science Gmbh Process for the production of polyunsaturated fatty acids in transgenic organisms
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JP4567047B2 (ja) 2004-02-27 2010-10-20 ビーエーエスエフ プラント サイエンス ゲーエムベーハー トランスジェニック植物における多価不飽和脂肪酸の製造方法
AR059376A1 (es) 2006-02-21 2008-03-26 Basf Plant Science Gmbh Procedimiento para la produccion de acidos grasos poliinsaturados
CA2768082C (en) 2009-07-17 2020-07-21 Basf Plant Science Company Gmbh Novel fatty acid desaturases and uses thereof
WO2015054138A1 (en) 2013-10-10 2015-04-16 William Marsh Rice University Improved fatty acid productivity
MX2022002950A (es) * 2019-09-10 2022-06-08 Perfect Day Inc Composiciones que comprenden subconjuntos de lípidos de leche y métodos para producir los mismos.
CN113773962B (zh) * 2021-08-16 2024-04-05 中山大学 一种定向灭活阿米巴体内细菌的方法及其应用

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Also Published As

Publication number Publication date
CN102597242B (zh) 2016-03-02
CA2763107A1 (en) 2010-12-16
DE112010002353T5 (de) 2012-08-09
EP2440662B1 (en) 2018-08-01
WO2010142522A3 (en) 2011-04-14
US8993841B2 (en) 2015-03-31
AU2010257729B2 (en) 2016-03-24
EP2440662A2 (en) 2012-04-18
CN102597242A (zh) 2012-07-18
WO2010142522A2 (en) 2010-12-16
BRPI1010614A2 (pt) 2016-10-25
AU2010257729A1 (en) 2012-01-12
US20120084889A1 (en) 2012-04-05
JP2012529277A (ja) 2012-11-22

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