CL2013001201A1 - Construcciones moleculas que comprenden un polinucleotido que codifica una acetilxiliano esterasa heteróloga; método para diseñar una planta que presenta una acetilación reducida en las paredes celulares;y método para producir biocombustible. - Google Patents

Construcciones moleculas que comprenden un polinucleotido que codifica una acetilxiliano esterasa heteróloga; método para diseñar una planta que presenta una acetilación reducida en las paredes celulares;y método para producir biocombustible.

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Publication number
CL2013001201A1
CL2013001201A1 CL2013001201A CL2013001201A CL2013001201A1 CL 2013001201 A1 CL2013001201 A1 CL 2013001201A1 CL 2013001201 A CL2013001201 A CL 2013001201A CL 2013001201 A CL2013001201 A CL 2013001201A CL 2013001201 A1 CL2013001201 A1 CL 2013001201A1
Authority
CL
Chile
Prior art keywords
acetylxyl
esterase
heterologous
plant
design
Prior art date
Application number
CL2013001201A
Other languages
English (en)
Inventor
Miron Abramson
Ziv Shani
Oded Shoseyov
Original Assignee
Futuragene Israel Ltd
Yissum Res Dev Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Futuragene Israel Ltd, Yissum Res Dev Co filed Critical Futuragene Israel Ltd
Publication of CL2013001201A1 publication Critical patent/CL2013001201A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01072—Acetylxylan esterase (3.1.1.72)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CL2013001201A 2010-11-03 2013-04-30 Construcciones moleculas que comprenden un polinucleotido que codifica una acetilxiliano esterasa heteróloga; método para diseñar una planta que presenta una acetilación reducida en las paredes celulares;y método para producir biocombustible. CL2013001201A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US40959010P 2010-11-03 2010-11-03

Publications (1)

Publication Number Publication Date
CL2013001201A1 true CL2013001201A1 (es) 2013-10-25

Family

ID=45390142

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2013001201A CL2013001201A1 (es) 2010-11-03 2013-04-30 Construcciones moleculas que comprenden un polinucleotido que codifica una acetilxiliano esterasa heteróloga; método para diseñar una planta que presenta una acetilación reducida en las paredes celulares;y método para producir biocombustible.

Country Status (8)

Country Link
US (1) US20130227724A1 (es)
CN (1) CN103237895A (es)
AR (1) AR083708A1 (es)
BR (1) BR112013010825A2 (es)
CA (1) CA2815927A1 (es)
CL (1) CL2013001201A1 (es)
UY (1) UY33705A (es)
WO (1) WO2012059922A2 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013137810A1 (en) * 2012-03-14 2013-09-19 Swetree Technologies Ab Genetically modified plants having improved saccharification properties
CN104245930A (zh) 2012-04-23 2014-12-24 诺维信公司 具有葡萄糖醛酸酯酶活性的多肽以及编码其的多核苷酸
CN105925591B (zh) * 2016-04-22 2019-12-24 浙江农林大学 一种毛竹木聚糖合成关键基因PeIRX10的克隆及其应用
CN108299548B (zh) * 2017-01-12 2020-06-09 中国科学院遗传与发育生物学研究所 Bs1-ct蛋白在调控植物细胞壁木聚糖去乙酰化反应中的应用
EP3354738A1 (en) * 2017-01-30 2018-08-01 Kws Saat Se Transgenic maize plant exhibiting increased yield and drought tolerance
WO2018175900A2 (en) * 2017-03-23 2018-09-27 Colorado State University Research Foundation Synthetic desalination genetic circuit in plants
CN113832153B (zh) * 2021-09-07 2022-07-01 中国热带农业科学院三亚研究院 高粱启动子、制备方法及应用

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
CA1192510A (en) 1981-05-27 1985-08-27 Lawrence E. Pelcher Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom
JPS6054684A (ja) 1983-09-05 1985-03-29 Teijin Ltd 新規ｄνａ及びハイブリツドｄνａ
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
GB8608850D0 (en) 1986-04-11 1986-05-14 Diatech Ltd Packaging system
JPS6314693A (ja) 1986-07-04 1988-01-21 Sumitomo Chem Co Ltd 植物ウイルスｒｎａベクタ−
ES2060646T3 (es) 1987-02-09 1994-12-01 Lubrizol Genetics Inc Virus rna hibrido.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
UA48104C2 (uk) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5942430A (en) * 1996-02-16 1999-08-24 Diversa Corporation Esterases
US7087426B2 (en) * 1996-09-11 2006-08-08 Agrigenesis Biosciences Ltd. Materials and methods for the modification of plant lignin content
US20040016025A1 (en) * 2001-09-26 2004-01-22 Paul Budworth Rice promoters for regulation of plant expression
EP1573010A4 (en) * 2002-12-12 2007-10-24 Univ Australian METHODS AND MEANS FOR MODULATING BIOSYNTHESIS OF CELLULOSE IN FIBER-PRODUCING PLANTS
EP1862540B1 (en) * 2003-05-29 2011-09-14 Genencor International, Inc. Novel trichoderma genes
US7238512B2 (en) * 2003-10-17 2007-07-03 E. I. Du Pont De Nemours And Company Method to produce para-hydroxybenzoic acid in the stem tissue of green plants by using a tissue-specific promoter
JP2009528033A (ja) 2006-02-27 2009-08-06 イーデンスペース システムズ コーポレイション 改善されたバイオ燃料原料のためのエネルギー作物
WO2009033071A2 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
CN101903518B (zh) 2007-09-28 2016-08-03 诺维信公司 具有乙酰木聚糖酯酶活性的多肽和编码该多肽的多核苷酸
ES2490608T3 (es) * 2007-12-06 2014-09-04 Novozymes A/S Polipéptidos con actividad de esterasa de acetilxilano y polinucleótidos que codifican los mismos
WO2009108941A2 (en) * 2008-02-29 2009-09-03 University Of Central Florida Research Foundation, Inc. Production and use of plant degrading materials
MY152105A (en) * 2008-03-21 2014-08-15 Danisco Us Inc Hemicellulase enriched compositions for enhancing hydrolysis of biomass
US20110165635A1 (en) 2008-04-21 2011-07-07 Chromatin, Inc. Methods and materials for processing a feedstock
US20100017916A1 (en) * 2008-05-30 2010-01-21 Edenspace Systems Corporation Systems for reducing biomass recalcitrance
US20120023627A1 (en) 2008-06-04 2012-01-26 Edenspace Systems Corporation Plant gene regulatory elements
WO2009155601A2 (en) * 2008-06-20 2009-12-23 Edenspace Systems Corporation Processing cellulosic biomass
BRPI0914835B1 (pt) 2008-07-29 2019-04-24 Novozymes A/S Célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo e para produzir uma proteína, construções de ácido nucléico, e, vetor de expressão.
CN102112604B (zh) 2008-07-31 2015-09-30 诺维信公司 具有乙酰木聚糖酯酶活性的多肽和编码该多肽的多核苷酸

Also Published As

Publication number Publication date
WO2012059922A2 (en) 2012-05-10
CN103237895A (zh) 2013-08-07
UY33705A (es) 2012-04-30
AR083708A1 (es) 2013-03-13
US20130227724A1 (en) 2013-08-29
BR112013010825A2 (pt) 2019-09-24
WO2012059922A3 (en) 2012-07-19
CA2815927A1 (en) 2012-05-10

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